TBLASTN 2.2.28+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: X5.fasta 1,868 sequences; 23,962,143 total letters Query= ACT_YEAST P60010 Actin Length=375 Score E Sequences producing significant alignments: (Bits) Value scaffold-444 721 0.0 scaffold-17 721 0.0 scaffold-514 717 0.0 scaffold-693 376 3e-116 scaffold-170 374 1e-115 scaffold-96 356 2e-109 scaffold-423 356 2e-109 unplaced-1003 215 5e-69 unplaced-948 215 5e-69 unplaced-987 234 3e-67 unplaced-1039 191 8e-60 unplaced-925 191 8e-60 scaffold-277 188 9e-52 scaffold-199 180 5e-49 scaffold-105 62.8 1e-10 scaffold-115 45.4 4e-05 scaffold-361 45.1 5e-05 scaffold-157 30.4 1.7 scaffold-287 29.3 4.1 > scaffold-444 Length=851481 Score = 721 bits (1861), Expect = 0.0, Method: Compositional matrix adjust. Identities = 333/375 (89%), Positives = 363/375 (97%), Gaps = 0/375 (0%) Frame = -2 Query 1 MDSEVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGIMVGMGQKDSYVGDEAQS 60 M+ EVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQG+MVGMGQKDSYVGDEAQS Sbjct 165923 MEDEVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQS 165744 Query 61 KRGILTLRYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPMNPKSNREKMT 120 KRGILTL+YPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVL TEAP+NPK+NREKMT Sbjct 165743 KRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLCTEAPLNPKANREKMT 165564 Query 121 QIMFETFNVPAFYVSIQAVLSLYSSGRTTGIVLDSGDGVTHVVPIYAGFSLPHAILRIDL 180 QIMFETFNVPAFYV+IQAVLSLY+SGRTTGIVLDSGDGV+H VPIY G++LPHAILR+DL Sbjct 165563 QIMFETFNVPAFYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDL 165384 Query 181 AGRDLTDYLMKILSERGYSFSTTAEREIVRDIKEKLCYVALDFEQEMQTAAQSSSIEKSY 240 AGRDLTDYLMKIL+ERGYSF+TTAEREIVRDIKEKLCYVALDFE EMQTA+ SS++EKSY Sbjct 165383 AGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFE*EMQTASTSSALEKSY 165204 Query 241 ELPDGQVITIGNERFRAPEALFHPSVLGLESAGIDQTTYNSIMKCDVDVRKELYGNIVMS 300 ELPDGQVITIGNERFR PEALF PS LG+E+AG+ +TTYNSIMKCDVD+RK+LY NIV+S Sbjct 165203 ELPDGQVITIGNERFRCPEALFQPSFLGMEAAGVHETTYNSIMKCDVDIRKDLYANIVLS 165024 Query 301 GGTTMFPGIAERMQKEITALAPSSMKVKIIAPPERKYSVWIGGSILASLTTFQQMWISKQ 360 GGTTM+PGIA+R+QKEITALAPSSMK+K++APPERKYSVWIGGSILASL+TFQQMWISKQ Sbjct 165023 GGTTMYPGIADRVQKEITALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWISKQ 164844 Query 361 EYDESGPSIVHHKCF 375 EYDESGPSIVH KCF Sbjct 164843 EYDESGPSIVHRKCF 164799 > scaffold-17 Length=2125590 Score = 721 bits (1861), Expect = 0.0, Method: Compositional matrix adjust. Identities = 333/375 (89%), Positives = 363/375 (97%), Gaps = 0/375 (0%) Frame = -3 Query 1 MDSEVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGIMVGMGQKDSYVGDEAQS 60 M+ EVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQG+MVGMGQKDSYVGDEAQS Sbjct 893374 MEDEVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQS 893195 Query 61 KRGILTLRYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPMNPKSNREKMT 120 KRGILTL+YPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVL TEAP+NPK+NREKMT Sbjct 893194 KRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLCTEAPLNPKANREKMT 893015 Query 121 QIMFETFNVPAFYVSIQAVLSLYSSGRTTGIVLDSGDGVTHVVPIYAGFSLPHAILRIDL 180 QIMFETFNVPAFYV+IQAVLSLY+SGRTTGIVLDSGDGV+H VPIY G++LPHAILR+DL Sbjct 893014 QIMFETFNVPAFYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDL 892835 Query 181 AGRDLTDYLMKILSERGYSFSTTAEREIVRDIKEKLCYVALDFEQEMQTAAQSSSIEKSY 240 AGRDLTDYLMKIL+ERGYSF+TTAEREIVRDIKEKLCYVALDFE EMQTA+ SS++EKSY Sbjct 892834 AGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFE*EMQTASTSSALEKSY 892655 Query 241 ELPDGQVITIGNERFRAPEALFHPSVLGLESAGIDQTTYNSIMKCDVDVRKELYGNIVMS 300 ELPDGQVITIGNERFR PEALF PS LG+E+AG+ +TTYNSIMKCDVD+RK+LY NIV+S Sbjct 892654 ELPDGQVITIGNERFRCPEALFQPSFLGMEAAGVHETTYNSIMKCDVDIRKDLYANIVLS 892475 Query 301 GGTTMFPGIAERMQKEITALAPSSMKVKIIAPPERKYSVWIGGSILASLTTFQQMWISKQ 360 GGTTM+PGIA+R+QKEITALAPSSMK+K++APPERKYSVWIGGSILASL+TFQQMWISKQ Sbjct 892474 GGTTMYPGIADRVQKEITALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWISKQ 892295 Query 361 EYDESGPSIVHHKCF 375 EYDESGPSIVH KCF Sbjct 892294 EYDESGPSIVHRKCF 892250 Score = 67.0 bits (162), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 56/239 (23%), Positives = 106/239 (44%), Gaps = 24/239 (10%) Frame = +1 Query 3 SEVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGIMVGMGQKDSYVGDEAQSKR 62 S+ + +VID GS +AG+A D P+ VF ++V + + G D Y G + Sbjct 1426510 SQESPIVIDFGSWQLRAGYADTD-PQLVFDNLVSKTS*GIHIGGGD*CD-YSGARSA--- 1426674 Query 63 GILTLRYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPMNPKSNREK 118 + ++ + ++E H F N L+ E+ P++ TE N R Sbjct 1426675 ------FDFS*SLIVHPPNLELTLDHVFANLLKAQREKTLA*RPIVFTETLCNLN*CRRI 1426836 Query 119 MTQIMFETFNVPAFYVSIQAVLSLYSSGRTTG---------IVLDSGDGVTHVVPIYAGF 169 ++++MFE ++V + A+ S Y +G +G ++L SG +H++P+ G Sbjct 1426837 VSELMFECYSVGRLAYGVDALFSFYHNGGFSGDDKKKKRNALILSSGHNASHIIPVIDGG 1427016 Query 170 SLPHAILRIDLAGRDLTDYLMKILSERGYSFSTTAEREIVRDIKEKLCYVALDFEQEMQ 228 R+D+ GR T+++ K L + + ++ + C VA D++ +M Sbjct 1427017 VDYSHCKRLDIGGRSSTEFMQKHLLVKYPELNQKLSLMTTD*LRVEFCRVASDYKGDMN 1427193 Score = 58.2 bits (139), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 35/133 (26%), Positives = 71/133 (53%), Gaps = 7/133 (5%) Frame = +1 Query 248 ITIGNERFRAPEALFHPSVLGLESAGIDQ--TTYNSIMKCDVDVRKELYGNIVMSGGTTM 305 I + ++R++ PE LF P ++G ESAG+ + +T + + D+ + ++ N+ ++GG TM Sbjct 1428313 IKLSSDRYKVPEILFQPHLMGSESAGLSEMCSTLAKVYESDLS-KLQMMSNVFVTGGNTM 1428489 Query 306 FPGIAERMQKEITALAPSSMKVKIIAPPERKYSVWIGGS--ILASLTTFQ-QMWISKQEY 362 +PG R+ ++ + VK+ + W G S L L+ +++I+++ Y Sbjct 1428490 YPGFD*RLFNDLR*NVLCDVPVKVYRAQDSILDAWRGASKWALDHLSNKN*KVFITRK*Y 1428669 Query 363 DESGPSIVH-HKC 374 +E G + H+C Sbjct 1428670 EECGADYLQEHQC 1428708 > scaffold-514 Length=59281 Score = 717 bits (1851), Expect = 0.0, Method: Compositional matrix adjust. Identities = 332/375 (89%), Positives = 362/375 (97%), Gaps = 0/375 (0%) Frame = -2 Query 1 MDSEVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGIMVGMGQKDSYVGDEAQS 60 M+ EVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQG+MVGMGQKDSYVGDEAQS Sbjct 10368 MEDEVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQS 10189 Query 61 KRGILTLRYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPMNPKSNREKMT 120 KRGILTL+YPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVL TEAP+NPK+NREKMT Sbjct 10188 KRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLCTEAPLNPKANREKMT 10009 Query 121 QIMFETFNVPAFYVSIQAVLSLYSSGRTTGIVLDSGDGVTHVVPIYAGFSLPHAILRIDL 180 QIMFETFNVPAFYV+I AVLSLY+SGRTTGIVLDSGDGV+H VPIY G++LPHAILR+DL Sbjct 10008 QIMFETFNVPAFYVAI*AVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDL 9829 Query 181 AGRDLTDYLMKILSERGYSFSTTAEREIVRDIKEKLCYVALDFEQEMQTAAQSSSIEKSY 240 AGRDLTDYLMKIL+ERGYSF+TTAEREIVRDIKEKLCYVALDFE EMQTA+ SS++EKSY Sbjct 9828 AGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFE*EMQTASTSSALEKSY 9649 Query 241 ELPDGQVITIGNERFRAPEALFHPSVLGLESAGIDQTTYNSIMKCDVDVRKELYGNIVMS 300 ELPDGQVITIGNERFR PEALF PS LG+E+AG+ +TTYNSIMKCDVD+RK+LY NIV+S Sbjct 9648 ELPDGQVITIGNERFRCPEALFQPSFLGMEAAGVHETTYNSIMKCDVDIRKDLYANIVLS 9469 Query 301 GGTTMFPGIAERMQKEITALAPSSMKVKIIAPPERKYSVWIGGSILASLTTFQQMWISKQ 360 GGTTM+PGIA+R+QKEITALAPSSMK+K++APPERKYSVWIGGSILASL+TFQQMWISKQ Sbjct 9468 GGTTMYPGIADRVQKEITALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWISKQ 9289 Query 361 EYDESGPSIVHHKCF 375 EYDESGPSIVH KCF Sbjct 9288 EYDESGPSIVHRKCF 9244 > scaffold-693 Length=1268102 Score = 376 bits (965), Expect = 3e-116, Method: Compositional matrix adjust. Identities = 173/381 (45%), Positives = 261/381 (69%), Gaps = 15/381 (4%) Frame = -1 Query 5 VAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGIMVGMGQKDSYVGDEAQSKR 62 + +V DNG+G K GF+G + P A+FPS+VGRP R + + + KD VGDEA + R Sbjct 747830 MKVVVCDNGTGFVKCGFSGTNFPEAIFPSVVGRPIIRAEESVGDIQVKDIMVGDEAAALR 747651 Query 63 GILTLRYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPMNPKSNREKMTQI 122 IL + YP+E+GIV NW+DM +W HTFY L++ P+E +LLTE P+NP NREKM + Sbjct 747650 SIL*MSYPMENGIVRNWEDMLHVWDHTFYERLKIDPKECKILLTEPPLNPLKNREKMVET 747471 Query 123 MFETFNVPAFYVSIQAVLSLYSSGRTTGIVLDSGDGVTHVVPIYAGFSLPHAILRIDLAG 182 MFE + Y++IQAV++LY+ G TG+V+DSGDGVTH+VP++ GF+LPH R+D+AG Sbjct 747470 MFEKYQFDGVYIAIQAVMTLYAQGLMTGVVVDSGDGVTHIVPVFDGFALPHLTRRLDVAG 747291 Query 183 RDLTDYLMKILSERGYSFSTTAEREIVRDIKEKLCYVALDFEQEMQTAAQSSSIEKSYEL 242 RD+T YL+K+L RGY+F+ TA+ E VR IKEKLCYV D + E + A +++++ + Y L Sbjct 747290 RDVTRYLIKLLLLRGYAFNRTADFETVRQIKEKLCYVGYDLDLEKRLANETTTLVE*YTL 747111 Query 243 PDGQVITIGNERFRAPEALFHPSVLGLESAGIDQTTYNSIMKCDVDVRKELYGNIVMSGG 302 PDG+VI +G+ERF APEA+F P ++ +ES G+ + +N+I D+D+R ELY +IV+SGG Sbjct 747110 PDGRVIKVGSERFEAPEAMFQPHLVDVESPGVAELLFNTIQAADIDIRSELYKHIVLSGG 746931 Query 303 TTMFPGIAERMQKEITAL-----------APSSMKVKIIAPPERKYSVWIGGSILASL-- 349 ++M+PG+ R++KE+ L K++I PP R++ V++GG++L + Sbjct 746930 SSMYPGLPSRLEKEVKQLYL*RVLKGDVSRLEKFKIRIEDPPRRRHMVFLGGAVLGEIMK 746751 Query 350 TTFQQMWISKQEYDESGPSIV 370 + W+S+++Y E G ++ Sbjct 746750 NRTEGFWMSRKDYMERGVKVL 746688 > scaffold-170 Length=449377 Score = 374 bits (961), Expect = 1e-115, Method: Compositional matrix adjust. Identities = 173/381 (45%), Positives = 261/381 (69%), Gaps = 15/381 (4%) Frame = -3 Query 5 VAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGIMVGMGQKDSYVGDEAQSKR 62 + +V DNG+G K GF+G + P A+FPS+VGRP R + + + KD VGDEA + R Sbjct 175793 MKVVVCDNGTGFVKCGFSGTNFPEAIFPSVVGRPIIRAEESVGDIQVKDIMVGDEAAALR 175614 Query 63 GILTLRYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPMNPKSNREKMTQI 122 IL + YP+E+GIV NW+DM +W HTFY L++ P+E +LLTE P+NP NREKM + Sbjct 175613 SIL*MSYPMENGIVRNWEDMLHVWDHTFYERLKIDPKECKILLTEPPLNPLKNREKMVET 175434 Query 123 MFETFNVPAFYVSIQAVLSLYSSGRTTGIVLDSGDGVTHVVPIYAGFSLPHAILRIDLAG 182 MFE + Y++IQAV++LY+ G TG+V+DSGDGVTH+VP++ GF+LPH R+D+AG Sbjct 175433 MFEKYQFDGVYIAIQAVMTLYAQGLMTGVVVDSGDGVTHIVPVFDGFALPHLTRRLDVAG 175254 Query 183 RDLTDYLMKILSERGYSFSTTAEREIVRDIKEKLCYVALDFEQEMQTAAQSSSIEKSYEL 242 RD+T YL+K+L RGY+F+ TA+ E VR IKEKLCYV D + E + A +++++ + Y L Sbjct 175253 RDVTRYLIKLLLLRGYAFNRTADFETVRQIKEKLCYVGYDLDLEKRLANETTTLVE*YTL 175074 Query 243 PDGQVITIGNERFRAPEALFHPSVLGLESAGIDQTTYNSIMKCDVDVRKELYGNIVMSGG 302 PDG+VI +G+ERF APEA+F P ++ +ES G+ + +N+I D+D+R ELY +IV+SGG Sbjct 175073 PDGRVIKVGSERFEAPEAMFQPHLVDVESPGVAELLFNTI*AADIDIRSELYKHIVLSGG 174894 Query 303 TTMFPGIAERMQKEITAL-----------APSSMKVKIIAPPERKYSVWIGGSILASL-- 349 ++M+PG+ R++KE+ L K++I PP R++ V++GG++L + Sbjct 174893 SSMYPGLPSRLEKEVKQLYL*RVLKGDVSRLEKFKIRIEDPPRRRHMVFLGGAVLGEIMK 174714 Query 350 TTFQQMWISKQEYDESGPSIV 370 + W+S+++Y E G ++ Sbjct 174713 NRTEGFWMSRKDYMERGVKVL 174651 > scaffold-96 Length=364303 Score = 356 bits (914), Expect = 2e-109, Method: Compositional matrix adjust. Identities = 169/344 (49%), Positives = 239/344 (69%), Gaps = 8/344 (2%) Frame = +1 Query 35 VGRPRHQGIMVGMGQKDSYVGDEAQSKRGILTLRYPIEHGIVTNWDDMEKIWHHTFYNEL 94 +GRP+H IM G D +G +A + RG+ + YP+ HGI +W D E +W H Y ++ Sbjct 93892 IGRPKHTRIMAGSLS*DVLIGMDALNNRGLCRINYPL*HGITESWSDQELLWSHA-YQQM 94068 Query 95 RVAPEEHPVLLTEAPMNPKSNREKMTQIMFETFNVPAFYVSIQAVLSLYSSGRTTGIVLD 154 + E+HP+L+TEAP+NP+ NR+ M Q++FETFNVPA YVS+QAVL+LY+SGRTTG+VLD Sbjct 94069 GINCEDHPLLITEAPLNPRRNRDLMAQVLFETFNVPALYVSLQAVLALYASGRTTGVVLD 94248 Query 155 SGDGVTHVVPIYAGFSLPHAILRIDLAGRDLTDYLMKILSE----RGYSFSTTAEREIVR 210 GDG +H VPIY GF++P AI R+++AGRD+T+Y+ +L + F T+AE ++ R Sbjct 94249 IGDGTSHCVPIYEGFAIPQAIHRVNIAGRDVTNYMAHLLRRGDACKALDFWTSAEMDLCR 94428 Query 211 DIKEKLCYVALDFEQEMQTAAQSSSIE-KSYELPDGQVITIGNERFRAPEALFHPSVLGL 269 IKEK YVA F Q + I+ + Y LPDGQV+ IG+ER+ APE LF P ++G Sbjct 94429 TIKEKCSYVA-SFGQLGPQ*SHLHDIDYEEYVLPDGQVVKIGSERYLAPEILFKPEIVGS 94605 Query 270 ESAGIDQTTYNSIMKCDVDVRKELYGNIVMSGGTTMFPGIAERMQKEITALAPSSMKVKI 329 E G+ + NSI K D+D+R+ LY NI++SGGTT+ G R+ E+ ++ +K+KI Sbjct 94606 ECVGVHEMLVNSINKVDLDLRRLLYENIILSGGTTLLKGFGSRLLAEVKQVSMRDVKIKI 94785 Query 330 IAPPERKYSVWIGGSILASLTTFQQMWISKQEYDESGPSIVHHK 373 APPERKYS WIGGSILASL+TF+++WI+ +EY+E P ++H K Sbjct 94786 FAPPERKYSTWIGGSILASLSTFKKLWITAEEYEED-PEVIHKK 94914 > scaffold-423 Length=1446679 Score = 356 bits (914), Expect = 2e-109, Method: Compositional matrix adjust. Identities = 168/344 (49%), Positives = 241/344 (70%), Gaps = 8/344 (2%) Frame = -2 Query 35 VGRPRHQGIMVGMGQKDSYVGDEAQSKRGILTLRYPIEHGIVTNWDDMEKIWHHTFYNEL 94 +GRP+H IM G +D +G +A + RG+ + YP++HGI +W D E +W H Y ++ Sbjct 1309263 IGRPKHTRIMAGSLSQDVLIGMDALNNRGLCRINYPLQHGITESWSD*ELLWSHA-YQQM 1309087 Query 95 RVAPEEHPVLLTEAPMNPKSNREKMTQIMFETFNVPAFYVSIQAVLSLYSSGRTTGIVLD 154 + E+HP+L+TEAP+NP+ NR+ M Q++FETFNVPA YVS+QAVL+LY+SGRTTG+VLD Sbjct 1309086 GINCEDHPLLITEAPLNPRRNRDLMAQVLFETFNVPALYVSLQAVLALYASGRTTGVVLD 1308907 Query 155 SGDGVTHVVPIYAGFSLPHAILRIDLAGRDLTDYLMKILSE----RGYSFSTTAEREIVR 210 GDG +H VPIY GF++P AI R+++AGRD+T+Y+ +L + F T+AE ++ R Sbjct 1308906 IGDGTSHCVPIYEGFAIPQAIHRVNIAGRDVTNYMAYLLRRGDACKAVDFWTSAEMDLCR 1308727 Query 211 DIKEKLCYVALDFEQEMQTAAQSSSIE-KSYELPDGQVITIGNERFRAPEALFHPSVLGL 269 IKEK Y+A F Q + I+ + Y LPDGQV+ IG+ER+ APE LF P ++G Sbjct 1308726 TIKEKCSYLA-SFGQLGP**SHLLDIDYEEYVLPDGQVVKIGSERYLAPEILFKPEIVGS 1308550 Query 270 ESAGIDQTTYNSIMKCDVDVRKELYGNIVMSGGTTMFPGIAERMQKEITALAPSSMKVKI 329 E G+ + NSI K D+D+R+ LY NI++SGGTT+ G R+ E+ ++ +K+KI Sbjct 1308549 ECVGVHEMLVNSINKVDLDLRRLLYENIILSGGTTLLKGFGSRLLAEVKQVSMRDVKIKI 1308370 Query 330 IAPPERKYSVWIGGSILASLTTFQQMWISKQEYDESGPSIVHHK 373 APPERKYS WIGGSILASL+TF+++WI+ +EY+E P ++H K Sbjct 1308369 FAPPERKYSTWIGGSILASLSTFKKLWITAEEYEED-PEVIHKK 1308241 > unplaced-1003 Length=332 Score = 215 bits (547), Expect = 5e-69, Method: Compositional matrix adjust. Identities = 98/110 (89%), Positives = 107/110 (97%), Gaps = 0/110 (0%) Frame = -2 Query 85 IWHHTFYNELRVAPEEHPVLLTEAPMNPKSNREKMTQIMFETFNVPAFYVSIQAVLSLYS 144 IWHHTFYNELRVAPEEHPVL TEAP+NPK+NREKMTQIMFETFNVPAFYV+IQAVLSLY+ Sbjct 331 IWHHTFYNELRVAPEEHPVLCTEAPLNPKANREKMTQIMFETFNVPAFYVAIQAVLSLYA 152 Query 145 SGRTTGIVLDSGDGVTHVVPIYAGFSLPHAILRIDLAGRDLTDYLMKILS 194 SGRTTGIVLDSGDGV+H VPIY G++LPHAILR+DLAGRDLTDYLMKIL+ Sbjct 151 SGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILT 2 > unplaced-948 Length=332 Score = 215 bits (547), Expect = 5e-69, Method: Compositional matrix adjust. Identities = 98/110 (89%), Positives = 107/110 (97%), Gaps = 0/110 (0%) Frame = +2 Query 85 IWHHTFYNELRVAPEEHPVLLTEAPMNPKSNREKMTQIMFETFNVPAFYVSIQAVLSLYS 144 IWHHTFYNELRVAPEEHPVL TEAP+NPK+NREKMTQIMFETFNVPAFYV+IQAVLSLY+ Sbjct 2 IWHHTFYNELRVAPEEHPVLCTEAPLNPKANREKMTQIMFETFNVPAFYVAIQAVLSLYA 181 Query 145 SGRTTGIVLDSGDGVTHVVPIYAGFSLPHAILRIDLAGRDLTDYLMKILS 194 SGRTTGIVLDSGDGV+H VPIY G++LPHAILR+DLAGRDLTDYLMKIL+ Sbjct 182 SGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILT 331 > unplaced-987 Length=26594 Score = 234 bits (596), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 143/422 (34%), Positives = 215/422 (51%), Gaps = 62/422 (15%) Frame = -3 Query 3 SEVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVG------------RPRHQGIMVGMGQK 50 S + +V+DNG+G K G+AG++ P+ + PS + + + G+ Sbjct 19275 SRLPPVVMDNGTGYTKLGYAGNNDPQFIIPSAIAVKESGGGAQASAAGKTGNVKKGIEDL 19096 Query 51 DSYVGDEAQSKRGILTLRYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPM 110 D Y+GDEA + + +L YPI HG + NWD ME+ W + + +R PE+H LLTE P+ Sbjct 19095 DFYIGDEAFANQKTYSL*YPIRHGQIENWDWMERYWE*SIFKYMRCEPEDHAFLLTEPPL 18916 Query 111 NPKSNREKMTQIMFETFNVPAFYVSIQAVL--------SLYSSGRTTGIVLDSGDGVTHV 162 N NRE +I FE+FNVP Y+++ AVL S S TG V+DSGDGVTHV Sbjct 18915 NAPENREYTAEIFFESFNVPGLYIAV*AVLALAASWTSSKVSDQTLTGCVIDSGDGVTHV 18736 Query 163 VPIYAGFSLPHAILRIDLAGRDLTDYLMKILSERGYSFSTTAEREIVRDIKEKLCYVALD 222 +P+ G+ + A+ I +AGRD+T ++ +L ER S E+ + IKE YV D Sbjct 18735 IPVAEGYVIGSALKHIPIAGRDITYFVQ*LLRERENSIPPEESLEVAKRIKEMYSYVCPD 18556 Query 223 FEQEMQT------------AAQSSSIEKSYELPDGQVITIGNERFRAPEALFHPSVLG-- 268 +E + S +K YE + +G ERF PE F P + Sbjct 18555 IVKEFKKFDAEPDKYFKKYVGHHSVTKKPYE------VDVGYERFLGPEIFFSPEIASAD 18394 Query 269 -LESAG--IDQTTYNSIMKCDVDVRKELYGNIVMSGGTTMFPGIAERMQKEI-------- 317 L G +D T NS +D R+ LY N+V+SGG+TM+ + R+Q++I Sbjct 18393 FLTPLGDVVD*TIQNS----PIDTRRGLYRNVVLSGGSTMYKDFSRRLQRDIKRLVDARQ 18226 Query 318 -------TALAPSSMKVKIIAPPERKYSVWIGGSILASLTTFQQMWISKQEYDESGPSIV 370 T + M+V +I+ +++Y+VW GGS+LA F +K +Y+E GPSI Sbjct 18225 KRSESLQTQIKAKPMEVNVISHKKQRYAVWFGGSLLADTPEFYSYCHTKADYEEYGPSIC 18046 Query 371 HH 372 + Sbjct 18045 RY 18040 > unplaced-1039 Length=270 Score = 191 bits (484), Expect = 8e-60, Method: Compositional matrix adjust. Identities = 86/90 (96%), Positives = 89/90 (99%), Gaps = 0/90 (0%) Frame = -1 Query 23 GDDAPRAVFPSIVGRPRHQGIMVGMGQKDSYVGDEAQSKRGILTLRYPIEHGIVTNWDDM 82 GDDAPRAVFPSIVGRPRHQG+MVGMGQKDSYVGDEAQSKRGILTL+YPIEHGIVTNWDDM Sbjct 270 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDM 91 Query 83 EKIWHHTFYNELRVAPEEHPVLLTEAPMNP 112 EKIWHHTFYNELRVAPEEHPVL TEAP+NP Sbjct 90 EKIWHHTFYNELRVAPEEHPVLCTEAPLNP 1 > unplaced-925 Length=270 Score = 191 bits (484), Expect = 8e-60, Method: Compositional matrix adjust. Identities = 86/90 (96%), Positives = 89/90 (99%), Gaps = 0/90 (0%) Frame = +1 Query 23 GDDAPRAVFPSIVGRPRHQGIMVGMGQKDSYVGDEAQSKRGILTLRYPIEHGIVTNWDDM 82 GDDAPRAVFPSIVGRPRHQG+MVGMGQKDSYVGDEAQSKRGILTL+YPIEHGIVTNWDDM Sbjct 1 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDM 180 Query 83 EKIWHHTFYNELRVAPEEHPVLLTEAPMNP 112 EKIWHHTFYNELRVAPEEHPVL TEAP+NP Sbjct 181 EKIWHHTFYNELRVAPEEHPVLCTEAPLNP 270 > scaffold-277 Length=563196 Score = 188 bits (478), Expect = 9e-52, Method: Compositional matrix adjust. Identities = 113/354 (32%), Positives = 175/354 (49%), Gaps = 59/354 (17%) Frame = -1 Query 75 IVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPMNPKSNREKMTQIMFETFNVPAFYV 134 +V W+ EK+W + +RVA E+PVL+ E N R+K+ ++ + + VPA Y Sbjct 452727 VVVEWELYEKLWEYAI-K*MRVAISEYPVLVVERSDNSADERQKVADLLIK*YQVPAIYF 452551 Query 135 SIQAVLSLYSSGRTTGIVLDSGDGVTHVVPIYAGFSLPHAILRIDLAGRDLT-------- 186 + AV++ +++GR++G+V+D G +T V P+Y G+ L + DLAG + Sbjct 452550 ARSAVMAAFAAGRSSGLVIDVGADLTTVTPVYEGYVLNKGAIHQDLAGNTINTRIYEHLT 452371 Query 187 ---------------------------DYLMKILSERGYSFSTTAEREIVRDIKEKLCYV 219 D +K L+ SF + IV D K+ +C V Sbjct 452370 AELQLNLTPRFKIEKKKALVVDVRTKPDLTLKKLNGVTESFEHHEKMRIVEDFKQSVCAV 452191 Query 220 ALDFEQEMQTAAQSSSIEKSYELPDGQVITIGNERFRAPEALFHPSVL------------ 267 + A + ++ SYE PDG T G ERF+ E LF P VL Sbjct 452190 SAARYDPQLLAMRPTT---SYEFPDGYNSTFGAERFKFGEMLFQPKVLAASHHHGDASGH 452020 Query 268 ----GLESAGIDQTTYNSIMKCDVDVRKELYGNIVMSGGTTMFPGIAERMQKEITALAPS 323 G+E G+ YNS+ CD DVR +L ++++ GG ++ AER+ E+ L P Sbjct 452019 HDNDGIEFQGVATLVYNSLNACDQDVRPQLLSHVIVVGGGSLLDQFAERLSVELGQLIPG 451840 Query 324 SMKVKIIAPP---ERKYSVWIGGSILASLTTFQQMWISKQEYDESGPSIVHHKC 374 +++KI A ER++ WIGGSIL+SL TFQ MW+++ EY++ G +IV KC Sbjct 451839 -LRIKIYASGNRVERRFGNWIGGSILSSLGTFQSMWVARSEYEDGGMTIVDKKC 451681 Score = 33.1 bits (74), Expect = 0.28, Method: Compositional matrix adjust. Identities = 13/33 (39%), Positives = 22/33 (67%), Gaps = 0/33 (0%) Frame = -2 Query 4 EVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVG 36 ++ ALV++ GS + G++G+D PR P+ VG Sbjct 452978 DIQALVVEVGSSSFRIGYSGEDTPRLAIPTAVG 452880 > scaffold-199 Length=1112851 Score = 180 bits (457), Expect = 5e-49, Method: Compositional matrix adjust. Identities = 112/357 (31%), Positives = 174/357 (49%), Gaps = 59/357 (17%) Frame = -2 Query 72 EHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPMNPKSNREKMTQIMFETFNVPA 131 E +V W+ EK+W + +RV E+PVL+ E N R+K+ ++ + + VPA Sbjct 75342 EFFVVVEWELYEKLWEYAI-K*MRVTISEYPVLVVERSDNSADERQKVADLLIK*YQVPA 75166 Query 132 FYVSIQAVLSLYSSGRTTGIVLDSGDGVTHVVPIYAGFSLPHAILRIDLAG--------- 182 Y + AV++ +++GR++G+V+D G +T + P+Y G+ L + DLAG Sbjct 75165 IYFARSAVMAAFAAGRSSGLVIDVGADLTTITPVYEGYVLTKGAVHQDLAGNAINTRIYE 74986 Query 183 --------------------------RDLTDYLMKILSERGYSFSTTAEREIVRDIKEKL 216 R D +K L+ SF + IV D K+ + Sbjct 74985 HLTSELQLNLTPRFKIEKKKALVVDVRTKPDLTLKKLNGVTESFEHHEKMRIVEDFKQSV 74806 Query 217 CYVALDFEQEMQTAAQSSSIEKSYELPDGQVITIGNERFRAPEALFHPSVL--------- 267 C V+ A + ++ SYE PDG T G ERF+ E LF P VL Sbjct 74805 CAVSAARYDPQLLAMRPTT---SYEFPDGYNSTFGAERFKFGEMLF*PKVLAASHHHGDA 74635 Query 268 -------GLESAGIDQTTYNSIMKCDVDVRKELYGNIVMSGGTTMFPGIAERMQKEITAL 320 G+E G+ YNS+ CD DVR L ++++ GG ++ AER+ E+ L Sbjct 74634 NGHHDNDGIEFQGVATLVYNSLNACD*DVRP*LLSHVIVVGGGSLLDQFAERLSVELGQL 74455 Query 321 APSSMKVKIIAPP---ERKYSVWIGGSILASLTTFQQMWISKQEYDESGPSIVHHKC 374 P +++KI A ER++ WIGGSIL+SL TFQ MW+++ EY++ G ++V KC Sbjct 74454 IPG-LRIKIYASGNRVERRFGNWIGGSILSSLGTFQSMWVARSEYEDGGMTVVDKKC 74287 Score = 34.7 bits (78), Expect = 0.075, Method: Compositional matrix adjust. Identities = 14/33 (42%), Positives = 23/33 (70%), Gaps = 0/33 (0%) Frame = -3 Query 4 EVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVG 36 ++ ALV++ GS + G++G+D PR V P+ VG Sbjct 75584 DIQALVVEVGSSSFRIGYSGEDTPRLVIPTAVG 75486 > scaffold-105 Length=655906 Score = 62.8 bits (151), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 36/133 (27%), Positives = 72/133 (54%), Gaps = 7/133 (5%) Frame = -3 Query 248 ITIGNERFRAPEALFHPSVLGLESAGIDQ--TTYNSIMKCDVDVRKELYGNIVMSGGTTM 305 I + ++R++ PE LF P ++G ESAG+ + +T + + D+D + ++ N+ ++GG TM Sbjct 585701 IKLSSDRYKVPEILFQPHLMGSESAGLSEMCSTLAKVYESDLD-KLQMMSNVFVTGGNTM 585525 Query 306 FPGIAERMQKEITALAPSSMKVKIIAPPERKYSVWIGGS--ILASLTTFQ-QMWISKQEY 362 + G +R+ ++ + VK+ + W G S L LT +++I+++ Y Sbjct 585524 YRGFDQRLFNDLRQNVLCDVPVKVYHAQDSILDAWRGASKWALDHLTNKN*KVFITRK*Y 585345 Query 363 DESGPSIVH-HKC 374 +E G + H+C Sbjct 585344 EECGADYLQEHQC 585306 > scaffold-115 Length=160031 Score = 45.4 bits (106), Expect = 4e-05, Method: Compositional matrix adjust. Identities = 58/290 (20%), Positives = 124/290 (43%), Gaps = 41/290 (14%) Frame = +2 Query 118 KMTQIMFETFNVPAFYVSIQAVLSLYSSGR-TTGIVLDSGDGVTHVVPIYAGFSLPHAIL 176 K+ ++ +E F V FYV +LSLY G+ +G++L G + I S+ ++ Sbjct 21809 KIIKLFYEIFQVDEFYV*STPLLSLYGYGQHLSGVLLYFGMSTCQLDCI-TDTSITYSK* 21985 Query 177 RIDLAGRDLTDYLMKILSERGYSFSTTAEREIVRDIKEKLCYV--------ALDFEQEMQ 228 ++ +L L+K L + E+V + + +C V A E++ Sbjct 21986 LSFMSCDNLRSLLVKAL*SDKLFKGNALDAELVSYVYDNICQVHPTGVIA*A***EKQDS 22165 Query 229 TAAQSSSIEKSYELPDGQVITIGNERFRAPEALFHPSVLGLESAGIDQTTYNSIMKCDVD 288 A++ I+ +Y+ + + + R E+LF + G+ + Y+++ CD++ Sbjct 22166 PASKK**IKFTYKDVE---YALNDAHLRVGESLFKGTN---GQTGLTKLIYDAVYSCDLE 22327 Query 289 VRKELYGNIVMSG----GTTMFPGIAERMQKEITALAPSSMKVKIIAPPERKY------- 337 R +L NI++ G G R++ E+ +S + + P K+ Sbjct 22328 KRVQLLDNIIIDGHLPSGRKFRESFRLRLELEMKKYLCNSEFLNELQPRYVKFGQFPSYL 22507 Query 338 -------------SVWIGGSILASLT-TFQQMWISKQEYDESGPSIVHHK 373 S +IG S+++ L + ++++ +Y++ GP ++H + Sbjct 22508 GEEVSNTQ*SLYISPYIGASVISKLVFNDNKNYLTRADYEQYGPKLLHDR 22657 > scaffold-361 Length=455836 Score = 45.1 bits (105), Expect = 5e-05, Method: Compositional matrix adjust. Identities = 60/292 (21%), Positives = 125/292 (43%), Gaps = 41/292 (14%) Frame = -1 Query 118 KMTQIMFETFNVPAFYVSIQAVLSLYSSGR-TTGIVLDSGDGVTHVVPIYAGFSLPHAIL 176 K+ ++ +ETF + FYV +LSLY G+ +G+++ G + I S+ ++ Sbjct 229618 KIIKLFYETFQLDEFYV*STPLLSLYGYGQHLSGVLMYFGMSAC*LDCI-TDTSITYSK* 229442 Query 177 RIDLAGRDLTDYLMKILSERGYSFSTTAEREIVRDIKEKLCYVALDF--------EQEMQ 228 L+ +L L+K L + E+V I + +C V E++ Sbjct 229441 MNSLSCDNLRSILVKAL*SDKLFKGNALDSELVSFIYDNICQVHPTGVIA*S***EKQDS 229262 Query 229 TAAQSSSIEKSYELPDGQVITIGNERFRAPEALFHPSVLGLESAGIDQTTYNSIMKCDVD 288 A++ I+ +Y+ + T+ + R E+LF V G G+ + Y+++ CD++ Sbjct 229261 PASKKQ*IKFTYKDVE---YTLNDAHLRIGESLF-KGVNG--QGGLTKLIYDAVYSCDLE 229100 Query 289 VRKELYGNIVMSG----GTTMFPGIAERMQKEITALAPSSMKVKIIAPPERKY------- 337 R +L NI++ G G R++ E+ +S + + P K+ Sbjct 229099 KRVQLLDNIIIDGHLPSGRKFRESFRLRLEMEMKKFLCNSEFLNEL*PRYVKFGQFPSYL 228920 Query 338 -------------SVWIGGSILASLT-TFQQMWISKQEYDESGPSIVHHKCF 375 S +IG +++ L + ++++ +Y++ GP +H + + Sbjct 228919 GEEVSNTQ*SLYISPYIGAGVISKLVFNDNKNYLTRADYEQYGPKQLHDRLY 228764 > scaffold-157 Length=706690 Score = 30.4 bits (67), Expect = 1.7, Method: Compositional matrix adjust. Identities = 10/19 (53%), Positives = 16/19 (84%), Gaps = 0/19 (0%) Frame = +3 Query 70 PIEHGIVTNWDDMEKIWHH 88 P EH I+TN DD+++I++H Sbjct 214734 PTEHSILTNTDDLQEIYYH 214790 > scaffold-287 Length=687983 Score = 29.3 bits (64), Expect = 4.1, Method: Compositional matrix adjust. Identities = 18/62 (29%), Positives = 29/62 (47%), Gaps = 8/62 (13%) Frame = -3 Query 151 IVLDSGDGVTHVVPIYAGFSLPHAILRIDLAGRDLTDYL-MKILSERGYSFSTTAEREIV 209 ++ D GDGV P Y GF + D+ R + + + SE GY+ + RE+ Sbjct 565008 VLCDDGDGVLIFTPFYGGFCM-------DVELRAHVKLIHVPLSSEDGYALNADKLREVY 564850 Query 210 RD 211 +D Sbjct 564849 KD 564844 Lambda K H a alpha 0.318 0.135 0.396 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 2145159775 Database: X5.fasta Posted date: Nov 8, 2020 10:15 PM Number of letters in database: 23,962,143 Number of sequences in database: 1,868 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 13 Window for multiple hits: 40