FastQCFastQC Report
Вс 21 дек 2014
Ath_tae_CTTGTA_L003_R2_002_cleaned.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameAth_tae_CTTGTA_L003_R2_002_cleaned.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3844518
Sequences flagged as poor quality0
Sequence length50-101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGGG2603.945297E-717.9532178
TGGCCCG3655.1879397E-815.3501055
GGCCCGG3353.2958815E-715.3266266
CGCGGCC2601.0004442E-1014.36294836-37
CGGCCGC2753.5779522E-912.72897338-39
GCCCGGG3701.5424528E-512.6157737
CCGGGGG4256.362667E-510.9831459
GCGGCCG3203.3671313E-810.94052636-37
GGCCGCG2603.820289E-610.77066938-39
TCGCGGC3854.3964974E-910.305881534-35
GCCGCGT3203.9659608E-710.20836840-41
GGGCCAT7553.5448465E-89.8942921
GCCATAC7603.9213774E-89.8299663
ATGGCCC5701.1361197E-59.8294534
TATCGCG4302.6348607E-78.68468632-33
CCGCGTC3305.966573E-47.77780540-41
ATCGCGG4851.6347476E-67.69972434-35
GTATGGC8057.7474106E-57.5403772
GGCCATA9307.4092422E-67.53102162
CGCGTCT4058.423592E-57.489932542-43