FastQCFastQC Report
Вт 9 дек 2014
Ath_tae_CTTGTA_L003_R2_002.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameAth_tae_CTTGTA_L003_R2_002.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCCG3755.847869E-815.2011365
GGCCCGG3851.9435181E-512.3350346
CGCCGTA5300.011.20212546-47
GCCCGGG4509.5191586E-510.5559487
GGGCGCC2157.3632196E-49.93961942-43
GAGCGTC7351.9757317E-79.6942379
GCGGCCG3305.1487223E-69.35693526-27
CCCGGGG5204.0836452E-49.1349548
ATGGCCC6959.912301E-68.8854444
GTACTGG7101.2996901E-58.6968541
CGCGGCC3000.0016930617.916267424-25
AGCGTCG7351.0004442E-107.755389710-11
GGTCGCC5052.3171797E-67.52305542-43
GTATGGC8004.8562433E-47.1255322
GTCGCCG7903.6798156E-96.914078744-45
TCGGGGG6251.0766016E-66.839013638-39
CCGGGGG6950.00710392466.8347869
TCGGAAG15551.371809E-76.415353
AGAGCGT12404.7933754E-56.129268
TGGTCGC6601.8210103E-56.11604542-43
Produced by FastQC (version 0.11.2)