FastQCFastQC Report
Пт 13 мар 2015
out.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameout.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2324119
Sequences flagged as poor quality0
Sequence length50-101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5450.023.23343748-49
TATGCCG6000.021.85253346-47
AGAGCAC10200.020.1798118
GCCGTCT5050.019.50177448-49
GAGCACA11150.018.8800099
CGTATGC6900.018.6315544-45
CACACGT7300.018.26360912-13
ATGCCGT5700.018.07352446-47
GCACACG5750.017.89861510-11
TCGGAAG11000.017.8428063
GTATGCC5900.017.43155344-45
GATCGGA12900.016.3124451
CGTCTGA8500.015.95455316-17
AGTCACC8500.015.13094628-29
CACGTCT9150.014.82404214-15
CGGAAGA14250.014.4292694
CCGTCTT9450.013.91572450-51
ATCGGAA14850.013.8462672
CCAGTCA9650.013.80930126-27
AGCACAC9350.013.75896210-11
Produced by FastQC (version 0.11.2)