#1 merges all fasta-files in particular directory into file j.fasta cat /P/y19/term3/block2/pr9/to_join/* > j.fasta #2 splits file with several fasta-sequences into several files seqretsplit /P/y19/term3/block2/pr9/to_split/coding1.fasta -auto #4 translates nucleotide sequences to file p.fasta transeq /P/y19/term3/block2/pr9/to_split/coding1.fasta -out p.fasta #6 changes format from fasta to msf, applied to alignment file seqret /P/y19/term3/block2/pr9/alignment.fasta -osformat2 msf -out alignment.msf #10 shuffles nucleotide sequence in file 4.fasta shuffleseq 4.fasta -auto #11 writes 3 random sequences with length=100 into file makeseq.fasta makenucseq -amount 3 -auto #15 deletes ctrl+r symbol in the end of stdin, hence translates stdin from dos format to unix and outputs to stdout dos2unix