1: Development. 2007 Jul;134(14):2593-603. Epub 2007 Jun 6.

Erratum in:
    Development. 2008 Feb;135(4):786.

Oocyte-derived BMP15 and FGFs cooperate to promote glycolysis in cumulus cells.

Sugiura K, Su YQ, Diaz FJ, Pangas SA, Sharma S, Wigglesworth K, O'Brien MJ,
Matzuk MM, Shimasaki S, Eppig JJ.

The Jackson Laboratory, Bar Harbor, ME 04609, USA.

Mammalian oocytes are deficient in their ability to carry out glycolysis.
Therefore, the products of glycolysis that are necessary for oocyte development
are provided to oocytes by companion cumulus cells. Mouse oocytes secrete
paracrine factors that promote glycolysis in cumulus cells. The objective of this
study was to identify paracrine factors secreted by oocytes that promote
glycolysis and expression of mRNA encoding the glycolytic enzymes PFKP and LDHA. 
Candidates included growth differentiation factor 9 (GDF9), bone morphogenetic
protein 15 (BMP15) and fibroblast growth factors (FGFs). Bmp15-/- and Gdf9+/-
Bmp15-/- (double mutant, DM) cumulus cells exhibited reduced levels of both
glycolysis and Pfkp and Ldha mRNA, and mutant oocytes were deficient in promoting
glycolysis and expression of Pfkp and Ldha mRNA in cumulus cells of wild-type
(WT) mice. Alone, neither recombinant BMP15, GDF9 nor FGF8 promoted glycolysis
and expression of Pfkp and Ldha mRNA in WT cumulus cells. Co-treatment with BMP15
and FGF8 promoted glycolysis and increased expression of Pfkp and Ldha mRNA in WT
cumulus cells to the same levels as WT oocytes; however, the combinations of
BMP15/GDF9 or GDF9/FGF8 did not. Furthermore, SU5402, an FGF receptor-dependent
protein kinase inhibitor, inhibited Pfkp and Ldha expression in cumulus cells
promoted by paracrine oocyte factors. Therefore, oocyte-derived BMP15 and FGFs
cooperate to promote glycolysis in cumulus cells.


PMID: 17553902 [PubMed - indexed for MEDLINE]

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