[0.000000] Reading graph file ./Graph [0.000029] Graph has 286 nodes and 7053346 sequences [0.022580] Measuring median coverage depth... [0.022651] Median coverage depth = 37.524173 [0.022683] Reading read set file ./Sequences; [0.637471] 7053346 sequences found [4.566143] Done [7.334102] Removing contigs with coverage < 18.762086... [7.334164] Concatenation... [7.404918] Renumbering nodes [7.404941] Initial node count 286 [7.404944] Removed 234 null nodes [7.404946] Concatenation over! [7.404949] Concatenation... [7.404951] Renumbering nodes [7.404952] Initial node count 52 [7.404954] Removed 0 null nodes [7.404955] Concatenation over! [7.404957] Clipping short tips off graph, drastic [7.404961] Concatenation... [7.404964] Renumbering nodes [7.404965] Initial node count 52 [7.404967] Removed 4 null nodes [7.404968] Concatenation over! [7.404969] 48 nodes left [7.404971] WARNING: NO EXPECTED COVERAGE PROVIDED [7.404973] Velvet will be unable to resolve any repeats [7.404974] See manual for instructions on how to set the expected coverage parameter [7.404976] Concatenation... [7.404978] Renumbering nodes [7.404979] Initial node count 48 [7.404980] Removed 0 null nodes [7.404982] Concatenation over! [7.404983] Removing reference contigs with coverage < 18.762086... [7.404991] Concatenation... [7.404995] Renumbering nodes [7.404996] Initial node count 48 [7.404998] Removed 0 null nodes [7.405002] Concatenation over! [7.431189] Writing contigs into ./contigs.fa... [7.476606] Writing into stats file ./stats.txt... [7.476960] Writing into graph file ./LastGraph... [7.533508] Estimated Coverage cutoff = 18.762086 Final graph has 48 nodes and n50 of 65554, max 111962, total 654072, using 0/7053346 reads