TBLASTN 2.2.28+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: gt_genome.fasta
           1 sequences; 3,550,319 total letters



Query= TRMB_BACSU O34522 tRNA (guanine-N(7)-)-methyltransferase (2.1.1.33)
(BsTrmB) (tRNA (guanine(46)-N(7))-methyltransferase)
(tRNA(m7G46)-methyltransferase)

Length=213
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

  CP000557 CP000557.1 Geobacillus thermodenitrificans NG80-2, com...   265    3e-81


> CP000557 CP000557.1 Geobacillus thermodenitrificans NG80-2, complete 
genome.
Length=3550319

 Score =  265 bits (678),  Expect = 3e-81, Method: Compositional matrix adjust.
 Identities = 122/209 (58%), Positives = 155/209 (74%), Gaps = 0/209 (0%)
 Frame = -3

Query  1        MRMRHKPWADDFLAENADIAISNPADYKGKWNTVFGNDNPIHIEVGTGKGQFISGMAKQN  60
                MR+R+KPWA D +A      I +P   +G+W+ +FGND PIHIE+GTGKG+FI+ MAK +
Sbjct  2845866  MRLRNKPWAKDKIAAYPQYVIPDPEARRGRWHELFGNDRPIHIEIGTGKGKFITEMAKLH  2845687

Query  61       PDINYIGIELFKSVIVTAVQKVKDSEAQNVKLLNIDADTLTDVFEPGEVKRVYLNFSDPW  120
                PD+N+IGIEL+ SV+V+A+ K+ +SE  NV+LLN +A  LT  F  GE+ R+YLNFSDPW
Sbjct  2845686  PDVNFIGIELYPSVLVSALDKLIESELPNVRLLNANAKDLTSFFADGEIARIYLNFSDPW  2845507

Query  121      PKKRHEKRRLTYSHFLKKYEEVMGKGGSIHFKTDNRGLFEYSLKSFSEYGLLLTYVSLDL  180
                PKKRHEKRRLTY  FL  Y+ ++   G IH KTDN+  FEYSL S S+YG +L  V LDL
Sbjct  2845506  PKKRHEKRRLTYRDFLALYDRILSADGDIHLKTDNQSFFEYSLVSLSQYGFVLAAVQLDL  2845327

Query  181      HNSNLEGNIMTEYEEKFSALGQPIYRAEV  209
                H S++ GN+MTEYEEKFSA G  IYR E 
Sbjct  2845326  HRSDIVGNVMTEYEEKFSAKGNRIYRCEA  2845240



Lambda      K        H        a         alpha
   0.317    0.135    0.404    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 156203256


  Database: gt_genome.fasta
    Posted date:  Dec 15, 2013  2:51 AM
  Number of letters in database: 3,550,319
  Number of sequences in database:  1



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40