FastQCFastQC Report
Ον 29 δεκ 2014
output.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenameoutput.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3758903
Sequences flagged as poor quality0
Sequence length50-101
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGGC3450.002539852467.6805953
GGACGAC3650.00474526442.680226
GAGCACG4950.029.5741429
TGCCGTC8700.023.35193348-49
GCACGCG2800.023.07109810-11
ACGCGTC2900.022.27925912-13
CACGCGT3100.022.23134812-13
TATGCCG9450.021.65320646-47
CAAAGCG9250.021.4118679
AGAGCAC15550.020.9478768
CGTATGC10450.019.92432844-45
GCCGTCT8250.019.60002748-49
CAGGGTG4300.001796635318.1279541
ATGGGGA13355.6306645E-417.4904944
ATGCCGT9400.017.3707346-47
GACGCCA4653.5519985E-416.7382134
GCGTTGT4754.394889E-416.3858284
GTATGCC10600.016.33623744-45
CGTCTGA12650.015.820641516-17
ATGACGT9903.875114E-415.7475141
Produced by FastQC (version 0.11.2)