LOCUS       KJ893891                 438 bp    DNA     linear   SYN 19-MAR-2015
DEFINITION  Synthetic construct Homo sapiens clone ccsbBroadEn_03285 AHSP gene,
            encodes complete protein.
ACCESSION   KJ893891
VERSION     KJ893891.1
KEYWORDS    ORFeome Collaboration; Gateway cloning system; full-length ORF
            without stop codon.
SOURCE      synthetic construct
  ORGANISM  synthetic construct
            other sequences; artificial sequences.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Yang,X., Boehm,J.S., Yang,X., Salehi-Ashtiani,K., Hao,T., Shen,Y.,
            Lubonja,R., Thomas,S.R., Alkan,O., Bhimdi,T., Green,T.M.,
            Johannessen,C.M., Silver,S.J., Nguyen,C., Murray,R.R.,
            Hieronymus,H., Balcha,D., Fan,C., Lin,C., Ghamsari,L., Vidal,M.,
            Hahn,W.C., Hill,D.E. and Root,D.E.
  TITLE     A public genome-scale lentiviral expression library of human ORFs
  JOURNAL   Nat. Methods 8 (8), 659-661 (2011)
   PUBMED   21706014
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  AUTHORS   Root,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2014) Broad Institute, 75 Ames St., Cambridge, MA
            02142, USA
COMMENT     This clone is distributed via members of the ORFeome Collaboration
            (http://www.orfeomecollaboration.org/), including the
            Dana-Farber/Harvard Cancer Center (DF/HCC) DNA Resource Core DNA
            Repository
            (http://www.dfhcc.harvard.edu/core-facilities/dnaresource/) and the
            DNASU Plasmid Repository at ASU Biodesign Institute
            (http://dnasu.asu.edu/DNASU/Home.jsp).  See also
            http://www.broadinstitute.org/rnai/public/ for additional
            information on this clone.
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="synthetic construct"
                     /mol_type="other DNA"
                     /db_xref="taxon:32630"
                     /clone="ccsbBroadEn_03285"
                     /focus
                     /note="derived from Homo sapiens; Vector:pDONR223; derived
                     from source materials corresponding to GenBank accession:
                     BC035842.1; indicated CDS derived from human cDNA clone"
     source          66..372
                     /organism="Homo sapiens"
                     /mol_type="other DNA"
                     /db_xref="taxon:9606"
     CDS             66..>372
                     /note="missing stop codon"
                     /codon_start=1
                     /transl_table=11
                     /product="AHSP"
                     /protein_id="AIC51476.1"
                     /translation="MALLKANKDLISAGLKEFSVLLNQQVFNDPLVSEEDMVTVVEDW
                     MNFYINYYRQQVTGEPQERDKALQELRQELNTLANPFLAKYRDFLKSHELPSHPPPSS
                     "
ORIGIN      
        1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
       61 ttggcatggc tcttcttaag gccaataagg atctcatttc cgcaggattg aaggagttca
      121 gcgttctgct gaatcagcag gtcttcaatg atcctctcgt ctctgaagaa gacatggtga
      181 ctgtggtgga ggactggatg aacttctaca tcaactatta caggcagcag gtgacagggg
      241 agccccaaga gcgagacaag gctctgcagg agcttcggca agagctgaac actctggcca
      301 accctttcct ggccaagtac agggacttcc tgaagtctca tgagctcccg agtcacccac
      361 cgccctcctc ctacccaact ttcttgtaca aagttggcat tataagaaag cattgcttat
      421 caatttgttg caacgaac
//