TBLASTN 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= sp|P04391|OTC1_ECOLI Ornithine carbamoyltransferase chain I OS=Escherichia coli (strain K12) GN=argI PE=1 SV=3 (334 letters) Database: xc 460 sequences; 5,103,728 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AE012332 AE008922 Xanthomonas campestris pv. campestris str. ATC... 125 5e-30 AE012388 AE008922 Xanthomonas campestris pv. campestris str. ATC... 65 8e-12 >AE012332 AE008922 Xanthomonas campestris pv. campestris str. ATCC 33913, section 240 of 460 of the complete genome. Length = 11470 Score = 125 bits (315), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 110/363 (30%), Positives = 161/363 (44%), Gaps = 35/363 (9%) Frame = -1 Query: 7 KHFLKLLDFTPAELNSXXXXXXXXXXXXXSGKEEAKLTGKNIALIFEKDSTRTRCSFEVA 66 KHFL D++ AEL++ K ++L GK+IAL+F S RTR SFE+ Sbjct: 7069 KHFLNTQDWSRAELDALLTQAALFK----RNKLGSELKGKSIALVFFNPSMRTRTSFELG 6902 Query: 67 AYDQGARVTYLGPSGS------QIG------HKESIKDTARVLGRMYDGIQYRGYGQ--- 111 A+ G L P +G +E I + ARVLGR D I R + + Sbjct: 6901 AFQLGGHAVVLQPGKDAWPIEFNLGTVMDGDTEEHIAEVARVLGRYVDLIGVRAFPKFVD 6722 Query: 112 -------EIVETLAEYASVPVWNGLTNEFHPTQLLADLLTMQEH-----LPGKAFNEMTL 159 +++++ A+Y+ VPV N + HP Q LA L +QEH L GK + +T Sbjct: 6721 WSKDREDQVLKSFAKYSPVPVIN-METITHPCQELAHALALQEHFGTPDLRGKKY-VLTW 6548 Query: 160 VYAGDARNN-MGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQN----GGNIT 214 Y N + NS L A G+D+ L+ P P+ L A QN GG++ Sbjct: 6547 TYHPKPLNTAVANSALTIATRMGMDVTLLCPT---PDYILDERYMDWAAQNVAESGGSLQ 6377 Query: 215 LTEDVAKGVEGADFIYTDVWVSMGEAKEKWAERIALLREYQ---VNSKMMQLTGNPEVKF 271 ++ D+ GAD +Y W ++ W + +YQ V+ + M LT N F Sbjct: 6376 VSHDIDSAYAGADVVYAKSWGAL-PFFGNWEPEKPIRDQYQHFIVDERKMALTNN--GVF 6206 Query: 272 LHCLPAFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVAT 331 HCLP L ++ TD V +S I D+AENR+H KA+M A Sbjct: 6205 SHCLP------------------LRRNVKATDAVMDSPNCIAIDEAENRLHVQKAIMAAL 6080 Query: 332 LSK 334 + + Sbjct: 6079 VGQ 6071 >AE012388 AE008922 Xanthomonas campestris pv. campestris str. ATCC 33913, section 296 of 460 of the complete genome. Length = 10043 Score = 65.5 bits (158), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 78/296 (26%), Positives = 125/296 (42%), Gaps = 10/296 (3%) Frame = -2 Query: 43 LTGKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRM-Y 101 L G + +F + STRTR SF +AA GA V S S E+ +DT + L M Sbjct: 1801 LAGTAVCTLFFEPSTRTRSSFHLAAQRLGADVLNFDASTSSTRKGETARDTLKNLEAMGV 1622 Query: 102 DGIQYRGYGQEIVETLAEYA---SVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMT 158 G R VE LAE A + + G HPTQ L D+LT+++ G F+++ Sbjct: 1621 RGFVVRHPEDGAVERLAEAAGEGTALINAGDGRSAHPTQGLLDMLTLRQA-KGTDFSKLK 1445 Query: 159 LVYAGDARNN-MGNSMLEAAALTGL-DLRLVAPQACWPEAALVTECRALAQQNGGNITLT 216 +V GD +++ + S L A G ++R+ P + P+ ++ C + Sbjct: 1444 VVIVGDVKHSRVARSDLHALRTLGAGEIRVCGPASLLPDDDMLDGC-----------VVG 1298 Query: 217 EDVAKGVEGADFIYTDVWVSMGEAKEKWAE-RIALLREYQVNSKMMQ---LTGNPEVKFL 272 ED +EGAD + + +E+ E + L +Y + + + + L Sbjct: 1297 EDFDAMLEGADALMM-----LRLQRERMEEGLVPSLEQYHADYGLTRERLARAGRDAAVL 1133 Query: 273 HCLPAFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVM 328 H P ++ G+E+TDEV + A S V Q N + AV+ Sbjct: 1132 HPGP------------------INRGVEITDEVADGAQSCVLRQVANGVAVRMAVL 1019 Database: xc Posted date: Nov 4, 2009 3:14 PM Number of letters in database: 5,103,728 Number of sequences in database: 460 Lambda K H 0.317 0.132 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 460 Number of Hits to DB: 2,014,450 Number of extensions: 27781 Number of successful extensions: 213 Number of sequences better than 1.0e-03: 2 Number of HSP's gapped: 208 Number of HSP's successfully gapped: 2 Length of query: 334 Length of database: 1,701,242 Length adjustment: 88 Effective length of query: 246 Effective length of database: 1,660,762 Effective search space: 408547452 Effective search space used: 408547452 Neighboring words threshold: 13 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 46 (22.3 bits)