Running MakePangenome.. Information about input data: Number of genomes: 5 Number of sequences: 5 Number of sequences per genome: 1 Total length of input (bp): 28833898 Minimum genome length (bp): 5678205 Median genome length (bp): 5705580 Average genome length (bp): 5766779 Maximum genome length (bp): 5942969 ============================ [2021-Nov-15 12:47:28 UTC+03:00] BeginIteration 1, AnchorJoinerFast Length of all major blocks of at least 2 fragments: 302741 Stable blocks (represented in all genomes once, but not minor): fragments: 45 blocks: 9 Block identity: min: 0.9487 median: 0.9997 avg: 0.9892 max: 1 Identity of joined blocks: 0.999658 Nucleotides in blocks: 1044240 The percentage of input length: 3.62% ============================ [2021-Nov-15 12:48:43 UTC+03:00] BeginIteration 2, AnchorJoinerFast Length of all major blocks of at least 2 fragments: 3376098 Stable blocks (represented in all genomes once, but not minor): fragments: 950 blocks: 190 Block identity: min: 0.9455 median: 0.9998 avg: 0.9982 max: 1 Identity of joined blocks: 0.999648 Nucleotides in blocks: 14797008 The percentage of input length: 51.31% Distance from previous pre-pangenome (bp): 13836715 The percentage of input length: 47.99% Distance is sufficient to carry on ============================ [2021-Nov-15 12:50:48 UTC+03:00] BeginIteration 3, AnchorJoinerFast Length of all major blocks of at least 2 fragments: 4464094 Stable blocks (represented in all genomes once, but not minor): fragments: 1520 blocks: 304 Block identity: min: 0.9455 median: 0.9998 avg: 0.9986 max: 1 Identity of joined blocks: 0.999628 Nucleotides in blocks: 19592110 The percentage of input length: 67.94% Distance from previous pre-pangenome (bp): 4762636 The percentage of input length: 16.52% Distance is sufficient to carry on ============================ [2021-Nov-15 12:52:55 UTC+03:00] BeginIteration 4, AnchorJoinerFast Length of all major blocks of at least 2 fragments: 5144024 Stable blocks (represented in all genomes once, but not minor): fragments: 1700 blocks: 340 Block identity: min: 0.9128 median: 0.9998 avg: 0.9984 max: 1 Identity of joined blocks: 0.999645 Nucleotides in blocks: 22842858 The percentage of input length: 79.22% Distance from previous pre-pangenome (bp): 2731211 The percentage of input length: 9.47% Distance is sufficient to carry on ============================ [2021-Nov-15 12:55:03 UTC+03:00] BeginIteration 5, AnchorJoiner Length of all major blocks of at least 2 fragments: 5368190 Stable blocks (represented in all genomes once, but not minor): fragments: 1760 blocks: 352 Block identity: min: 0.9128 median: 0.9998 avg: 0.9984 max: 1 Identity of joined blocks: 0.999649 Nucleotides in blocks: 23875419 The percentage of input length: 82.8% Distance from previous pre-pangenome (bp): 1444002 The percentage of input length: 5.01% Distance is sufficient to carry on ============================ [2021-Nov-15 12:59:51 UTC+03:00] BeginIteration 6, AnchorJoiner Length of all major blocks of at least 2 fragments: 5366629 Stable blocks (represented in all genomes once, but not minor): fragments: 1755 blocks: 351 Block identity: min: 0.9128 median: 0.9998 avg: 0.9984 max: 1 Identity of joined blocks: 0.999649 Nucleotides in blocks: 23874838 The percentage of input length: 82.8% Distance from previous pre-pangenome (bp): 13709 The percentage of input length: 0.05% Distance is too low => stopping this loop ============================ [2021-Nov-15 13:02:57 UTC+03:00] BeginIteration 7, AnchorBlastJoiner Length of all major blocks of at least 2 fragments: 5364158 Stable blocks (represented in all genomes once, but not minor): fragments: 1800 blocks: 360 Block identity: min: 0.9128 median: 0.9998 avg: 0.9985 max: 1 Identity of joined blocks: 0.99965 Nucleotides in blocks: 23858345 The percentage of input length: 82.74% Distance from previous pre-pangenome (bp): 1224110 The percentage of input length: 4.25% Distance is sufficient to carry on ============================ [2021-Nov-15 13:06:55 UTC+03:00] BeginIteration 8, AnchorBlastJoiner Length of all major blocks of at least 2 fragments: 5365294 Stable blocks (represented in all genomes once, but not minor): fragments: 1805 blocks: 361 Block identity: min: 0.9128 median: 0.9998 avg: 0.9985 max: 1 Identity of joined blocks: 0.99965 Nucleotides in blocks: 23858980 The percentage of input length: 82.74% Distance from previous pre-pangenome (bp): 4767 The percentage of input length: 0.02% Distance is too low => stopping this loop ============================ [2021-Nov-15 13:15:56 UTC+03:00] StopInfo The pangenome has been constructed. Blocks' lengths: 5521290 major: 5504780 minor: 16510 ============================ All major blocks of at least 2 fragments: fragments: 12630 blocks: 1271 fragments / blocks: 9.937 Block identity: min: 0.8586 median: 0.9881 avg: 0.9681 max: 1 Identity of joined blocks: 0.997221 Block sizes: min: 2 median: 6 avg: 9.94 max: 73 Fragment lengths: min: 97 median: 198 avg: 2261.86 max: 117811 Fragment length spreading ((max - min) / avg) inside block: min: 0 median: 0 avg: 0.0017 max: 0.1122 GC content: min: 0.2678 median: 0.5018 avg: 0.4965 max: 0.7066 Nucleotides in blocks: 28567233 The percentage of input length: 99.07% Total length of blocks: 5368282 The percentage of total blocks' length: 97.22% ============================ Stable blocks (represented in all genomes once, but not minor): fragments: 1770 blocks: 354 fragments / blocks: 5 Block identity: min: 0.9128 median: 0.9998 avg: 0.9984 max: 1 Identity of joined blocks: 0.999607 Block sizes: 5 Fragment lengths: min: 100 median: 2496 avg: 13480.80 max: 117811 Fragment length spreading ((max - min) / avg) inside block: min: 0 median: 0 avg: 0.0002 max: 0.0095 GC content: min: 0.283 median: 0.4996 avg: 0.4822 max: 0.6346 Nucleotides in blocks: 23861022 The percentage of input length: 82.75% Total length of blocks: 4772645 The percentage of total blocks' length: 86.44% ============================ Partial blocks (represented once in subset of genomes): fragments: 505 blocks: 188 fragments / blocks: 2.6861 Block identity: min: 0.9177 median: 1 avg: 0.9948 max: 1 Identity of joined blocks: 0.996418 Block sizes: min: 2 median: 2 avg: 2.69 max: 4 Fragment lengths: min: 100 median: 405 avg: 1482.89 max: 19266 Fragment length spreading ((max - min) / avg) inside block: min: 0 median: 0 avg: 0.0001 max: 0.0065 GC content: min: 0.2765 median: 0.465 avg: 0.466 max: 0.6792 Nucleotides in blocks: 748857 The percentage of input length: 2.59% Total length of blocks: 269359 The percentage of total blocks' length: 4.87% ============================ Blocks with repeats (at least two copies in at least one genome): fragments: 10355 blocks: 729 fragments / blocks: 14.2043 Block identity: min: 0.8586 median: 0.9423 avg: 0.9466 max: 1 Identity of joined blocks: 0.96298 Block sizes: min: 2 median: 10 avg: 14.20 max: 73 Fragment lengths: min: 97 median: 173 avg: 382.17 max: 17057 Fragment length spreading ((max - min) / avg) inside block: min: 0 median: 0 avg: 0.0029 max: 0.1122 GC content: min: 0.2678 median: 0.5138 avg: 0.5114 max: 0.7066 Nucleotides in blocks: 3957354 The percentage of input length: 13.72% Total length of blocks: 326278 The percentage of total blocks' length: 5.9% ============================ Unique (blocks of 1 fragment but not minor): fragments: 91 blocks: 91 Fragment lengths: min: 100 median: 432 avg: 1499.98 max: 23656 GC content: min: 0.3597 median: 0.4603 avg: 0.4724 max: 0.6944 Nucleotides in blocks: 136498 The percentage of input length: 0.47% Total length of blocks: 136498 The percentage of total blocks' length: 2.47% ============================ Minor blocks (too short to say smth about): fragments: 4310 blocks: 552 fragments / blocks: 7.8079 Block identity: min: 0 median: 1 avg: 0.783 max: 1 Identity of joined blocks: 0.902766 Block sizes: min: 1 median: 5 avg: 7.81 max: 55 Fragment lengths: min: 1 median: 19 avg: 30.20 max: 99 Fragment length spreading ((max - min) / avg) inside block: min: 0 median: 0 avg: 0.0181 max: 2.3333 GC content: min: 0 median: 0.5 avg: 0.5063 max: 1 Nucleotides in blocks: 130167 The percentage of input length: 0.45% Total length of blocks: 16510 The percentage of total blocks' length: 0.29% The pangenome has been prepared for the next step: PostProcessing .. done