TBLASTN 2.2.28+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: X5.fasta 1,868 sequences; 23,962,143 total letters Query= DNLI4_MOUSE Q8BTF7 DNA ligase 4 (6.5.1.1 {ECO:0000255|PROSITE-ProRule:PRU10135}) (DNA ligase IV) (Polydeoxyribonucleotide synthase [ATP] 4) Length=911 Score E Sequences producing significant alignments: (Bits) Value scaffold-243 285 3e-79 scaffold-100 276 3e-76 unplaced-1071 139 3e-33 scaffold-423 133 1e-31 scaffold-26 32.3 1.5 > scaffold-243 Length=448935 Score = 285 bits (728), Expect = 3e-79, Method: Compositional matrix adjust. Identities = 272/965 (28%), Positives = 445/965 (46%), Gaps = 128/965 (13%) Frame = +1 Query 49 FHDALHKNRKDVTDSFYPAMRLILPQLERERMAYGIKETMLAKLYIELLNLP----REGK 104 F DA R + + + +RLI+PQ R+ YG+KET L ++Y +L LP + K Sbjct 302302 FKDA----RNEAPEELFDIVRLIIPQ--RDPRTYGMKETRLVEIYSGMLALPHAEIKRLK 302463 Query 105 DAQ--KLLNYRTP--SGARTDAGDFAMIAYFVLKPRCLQKG-SLTIQQVNELLDLV---- 155 D + +L Y T A + DFA + ++ R G ++ I+ VN+ L+ + Sbjct 302464 DWK*PQLYQYTTDVYDDA*SSRIDFATVLQASIESRWTGVGETMNIRDVNDYLN*LN*LE 302643 Query 156 --ASNNSGKKKDLVKKS-----LLQLITQSSALEQKWLIRMIIKDLKLGISQQTIFSIFH 208 A + +G + ++ KK ++ S E KW+IR+IIKD+ +GI + ++ FH Sbjct 302644 KNAPSQNGFETNVPKKKK*EEIFYKIFNNCSPQE*KWIIRIIIKDMFIGIGEDSVLRTFH 302823 Query 209 NDAVELHNVTTDLEKVCRQLHDPSVGLSDISITLFSAFKPMLAAVADVERVEKDMKQQSF 268 DA E + TDL+KV + D D I LF+ F PM V + M + F Sbjct 302824 PDAPEEYKFCTDLQKVVDKCKDIHKRFKDAQI*LFT*FSPMKL*KVLPGDVVRKMDNEPF 303003 Query 269 YIETKLDGERMQMHKDGALYR--YFSRNGYNYTDQFGESPQEGS---LTPFIHNAFGTDV 323 YIE KLDG RM +H D + R YFSR+G +++ ++GES EG+ P + G V Sbjct 303004 YIEEKLDGFRMIVHLDV*MGRVTYFSRDGLDHSHEYGES*YEGASRRFWPCLTAKHG--V 303177 Query 324 QACILDGEMMAYNPTTQTFMQKGVK-FDIKRMVE--DSGLQTC----YSVFDVLMVNKKK 376 + ILD E++ +NP GV+ F R V D G+QT VFDVL +N Sbjct 303178 KNMILDTEILVFNPAND-----GVEAFGTIRQVAAYDDGIQTGKRLYLVVFDVLYLND*N 303342 Query 377 LGRETLRKRYEILSSTFTPIQGRIEIVQKTQAHTKKEVVDALNDAIDKREEGIMVKHPLS 436 L L KR L G +++++ +A T ++VV+ + + + K+ EGI+VK P S Sbjct 303343 LCMLPLSKRKSYLKDLIKEQHGIVKVLEY*EATTTEQVVNFMEEILLKKGEGIVVKDPKS 303522 Query 437 IYKPDKRGEGWLKIKPEYVSGLMDELDVLIVGGYWGKGSRGGMMSHFLCAVAETPPPGD- 495 Y PD R WLK+KP+Y+SGL + LD +I+G +G G + + + FL AV + D Sbjct 303523 TYIPDSRNGRWLKLKPDYISGLGEHLDAVIIGAKYGHGDKSNLYASFLVAVRDDRYESDT 303702 Query 496 ------RPSVFHTLCRVGSGYTMKELYDLGLKLAKYWKPFHKKSPPSSI----------- 538 + + TL VGSG++M +L ++ KL F PS + Sbjct 303703 GDDD*FSEA*WSTLSHVGSGFSMDQLAEMYHKLQPLEMRFDPDRIPSWLVAIPNPRLKRT 303882 Query 539 ----------LCGTEKPEVYI--EPQNSVIVQIKAAEIVPSDMYKTGSTLRFPRIEKIRD 586 L GT+ +V P +SVIV++K EI SD G LRFPR+ +IR Sbjct 303883 ASTNLTGKLMLIGTDNIDVLFAPSPSSSVIVELKCMEITTSDT-ACGYGLRFPRLVQIRS 304059 Query 587 DKEWHECMTLGDL-----------------EQLRGKASGKLATKHLHVGDDDEPREKRRK 629 DK + ++ L +L + AT + G P +KR++ Sbjct 304060 DKPLKDSLSYTQLAK*FE*TKGIKHHILRANELDDGSVFDTATAFMS-GSGSTPSKKRQR 304236 Query 630 PISKTKKAIRIIEHLKAPNLSNVNKVSNVFEDVEFCVMSGLDGYPKADLENRIAEFGGYI 689 P + +R + + + N+F+ +E V +G D + K D+E + + I Sbjct 304237 PKQELP*DLRGV------*VDEEEIMGNIFDGIEVFVANGSDEHSKQDIERELLKHKAVI 304398 Query 690 VQNPGPDTYCVIAGSENVRVKNIISSDKNDVVKPEWLLECFKTKTCVPWQPRFMIHMCPS 749 V NP T VIAG ++++V+ ++ + KP ++ +C + +P I++ Sbjct 304399 V*NPRISTQLVIAGKQSIKVRQLVDKK*CSIAKPSYVFDCIEADILLPLSESNSIYISDD 304578 Query 750 TKQHFA--REYDCYGDSYFVDTDLDQ----LKEVFL--GIKPSEQQTPEEMAPVIADLEC 801 + + A ++D YGD+Y D L + LKE+ + G E P ++ D Sbjct 304579 SAKTIADLDQFDEYGDNYAKDLSLKRFEHLLKEMTIE*G*IYGELNVPLDIGS--DD*SQ 304752 Query 802 RYS------------WDHSPLSMFRHYTIYLDLYAVINDLSS-RIEATRLGITALELRFH 848 R + DH + FR Y D+Y +I D ++ + L + Sbjct 304753 RQALL*LYE*LEM*PGDH---NFFRPVNAYFDMYEIIGDKEGVKVSFQSWNVLKLGVELF 304923 Query 849 GAKVVSCLSEGVSHVIIG-EDQRRVTDFK-IFRRMLKKKFKILQESWVSDSVDKGELQEE 906 G V + + +H++I +D RV + + L+++ I+ W+ D + + L +E Sbjct 304924 GGAVCNLIDH*TTHIVISPQDLSRVD*LRNVI*GRLEQRISIVSA*WLKDCIKERSLIDE 305103 Query 907 NQYLL 911 +YL+ Sbjct 305104 *RYLI 305118 > scaffold-100 Length=762135 Score = 276 bits (705), Expect = 3e-76, Method: Compositional matrix adjust. Identities = 268/966 (28%), Positives = 436/966 (45%), Gaps = 130/966 (13%) Frame = -3 Query 49 FHDALHKNRKDVTDSFYPAMRLILPQLERERMAYGIKETMLAKLYIELLNLPREGKDAQK 108 F DA R + D + +RLI+PQ R+ YG+KET L ++Y +L LP + ++ Sbjct 133285 FKDA----RNEAPDELFDIVRLIIPQ--RDPRTYGMKETRLVEIYSGMLALPH--AEIKR 133130 Query 109 LLNYRTP----------SGARTDAGDFAMIAYFVLKPRCLQKG-SLTIQQVNELLDLV-- 155 L +++ P A + DFA + ++ R G ++ I+ VN+ LD + Sbjct 133129 LKDWK*PQLY*YTTDVYDDA*SSRIDFATVLLASIESRWTGVGETMNIRDVNDYLD*LKQ 132950 Query 156 ----ASNNSGKKKDLVKKS-----LLQLITQSSALEQKWLIRMIIKDLKLGISQQTIFSI 206 A + +G + + KK ++ S E KW+IR+IIKD+ +GI + ++ Sbjct 132949 LEKNAPSQNGFETNAPKKKK*EEIFYKIFNNCSPQE*KWIIRIIIKDMFIGIGEDSVLRT 132770 Query 207 FHNDAVELHNVTTDLEKVCRQLHDPSVGLSDISITLFSAFKPMLAAVADVERVEKDMKQQ 266 FH DA E + TDL+KV + D D I LF+ F PM V + M + Sbjct 132769 FHPDAPEEYKFCTDLQKVVDKCKDIHKRFKDAQI*LFT*FSPMKL*KVLPGDVVRKMDNE 132590 Query 267 SFYIETKLDGERMQMHKDGALYR--YFSRNGYNYTDQFGESPQEGS---LTPFIHNAFGT 321 FYIE KLDG RM +H D + R YFSR+G +++ ++GES EG+ P + G Sbjct 132589 PFYIEEKLDGFRMIVHLDVQMGRVTYFSRDGLDHSHEYGES*YEGASRRFWPCLTAKQG- 132413 Query 322 DVQACILDGEMMAYNPTTQTFMQKGVK-FDIKRMVE--DSGLQTC----YSVFDVLMVNK 374 V+ ILD E++ +NP GV+ F R V D G+QT VFDVL +N Sbjct 132412 -VKNMILDTEILVFNPAND-----GVEAFGTIRQVAAYDDGIQTGKRLYLVVFDVLYLND 132251 Query 375 KKLGRETLRKRYEILSSTFTPIQGRIEIVQKTQAHTKKEVVDALNDAIDKREEGIMVKHP 434 L L KR + G +++++ +A T ++VV+ + + + K+ EGI+VK P Sbjct 132250 *NLCMLPLSKRKSYIKDLIEEQHGIVKVLEY*EATTTEQVVNFMEEILLKKGEGIVVKDP 132071 Query 435 LSIYKPDKRGEGWLKIKPEYVSGLMDELDVLIVGGYWGKGSRGGMMSHFLCAVAETPPPG 494 S Y PD R WLK+KP+Y+SGL + LD +I+G +G G + + + FL AV + Sbjct 132070 KSTYIPDSRNGRWLKLKPDYISGLGEHLDAVIIGAKYGHGDKSNLYASFLVAVRDDRYES 131891 Query 495 D-------RPSVFHTLCRVGSGYTMKELYDLGLKLAKYWKPFHKKSPPSSI--------- 538 D + TL VGSG+TM +L +L KL F PS + Sbjct 131890 DTGDDD*FSAD*WSTLSHVGSGFTMDQLAELYHKLQPLEMRFDPDRIPSWLVAIPNPQLR 131711 Query 539 ------------LCGTEKPEVYIEPQ--NSVIVQIKAAEIVPSDMYKTGSTLRFPRIEKI 584 L GT+ +V P SVIV++K EI SD G LRFPR+ I Sbjct 131710 RTASTNLTGKLMLIGTDNIDVLFAPLP*QSVIVELKCMEITTSDT-ACGYGLRFPRLV*I 131534 Query 585 RDDKEWHECMTLGDL----EQLRGKASGKLATKHLHVG------------DDDEPREKRR 628 R DK + ++ L E +G L L G P +KR+ Sbjct 131533 RSDKPLKDSLSYSQLAK*FE*TKGIKHHILRANELDDGSVIDTATALMSCSGSTPSKKRQ 131354 Query 629 KPISKTKKAIRIIEHLKAPNLSNVNKVSNVFEDVEFCVMSGLDGYPKADLENRIAEFGGY 688 +P + L+ + + N F+ +E V +G + + K +E + + Sbjct 131353 RP------K*ELP*DLRGV*VDEEEIMGNFFDGIEVFVANGSNEHSKQKIERELLKQKAV 131192 Query 689 IVQNPGPDTYCVIAGSENVRVKNIISSDKNDVVKPEWLLECFKTKTCVPWQPRFMIHMCP 748 IV NP T VIAG ++++V+ ++ + KP ++ +C + +P I + Sbjct 131191 IV*NPRVSTKLVIAGKQSIKVRQLVDKK*CSIAKPSYVFDCIEADILLPLTESNSIFISD 131012 Query 749 STKQHFA--REYDCYGDSYFVDTDLDQLKEVF--LGIKP----SEQQTPEEMAPVIADLE 800 + + A ++D YGD+Y D + + + + + I+ E P ++ AD Sbjct 131011 DSAKTIADLDQFDKYGDNYAKDLSMKRFEHLLKEMTIEE**IYGELNGPLDIGS--ADQL 130838 Query 801 CRYS------------WDHSPLSMFRHYTIYLDLYAVINDLSS-RIEATRLGITALELRF 847 R + DH + FR Y D+Y +I D + ++ + L + Sbjct 130837 HRQALL*LYE*LEM*PGDH---NFFRPVNAYFDMYKIIEDKEAVKVSPQLWTVLKLGVEL 130667 Query 848 HGAKVVSCLSEGVSHVIIG-EDQRRVTDFK-IFRRMLKKKFKILQESWVSDSVDKGELQE 905 G V + +H+++ +D RV + I + L+ + I+ W+ D + + L + Sbjct 130666 FGGAVCDSIDH*TTHIVVSPQDLSRVD*LRNINQGRLE*RISIVSS*WLKDCIKERSLID 130487 Query 906 ENQYLL 911 E +YL+ Sbjct 130486 EQRYLI 130469 > unplaced-1071 Length=86429 Score = 139 bits (349), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 154/622 (25%), Positives = 262/622 (42%), Gaps = 89/622 (14%) Frame = +1 Query 16 FADLCSTLERIQKGKDRAEKIRHFKEFLDSWRKFHDALHKNRKDVTDSFYPAMRLILPQL 75 F++L T E I+ R + F F + N KD+ Y + I P Sbjct 79483 FSELSQTFEEIEGTTKRLAILEILTTF------FVKVIRDNPKDLISVIYLCINRIGPTW 79644 Query 76 ERERMAYGIKETMLAKLYIELLNLPREGKDAQKLLNYRTPSGARTDAGDFAMIAYFVLK- 134 E + GI ET+L K P A+ + GD +A Sbjct 79645 EAAEL--GIGETVLIKAIASATGRPIPKIKAE-----------MHNLGDLGSVAQ*CRNN 79785 Query 135 -PRCLQKGSLTI----QQVNELLDLVASNNSGKKKDLVKKSLLQLITQSSALEQKWLIRM 189 P LT+ + + E+ + ++ KK D +K L+ E K++IRM Sbjct 79786 *PTMF*PKPLTV*TVFKTLTEISKITGHSSMQKKIDKIK----FLLVSCRDNETKFIIRM 79953 Query 190 IIKDLKLGISQQTIFSIFHNDAVELHNVTT---DLE-------KVCRQLHDPSVGLSDIS 239 + L++G+++ ++ + + +V N DLE +V +Q++ I Sbjct 79954 LEGKLRIGLAEASVLTALAHASVLSRNSELKR*DLEVELS*AVEVVKQVYSEVPMYERIV 80133 Query 240 ITLFSA----------------FKPMLA-AVADVERVEKDMKQQSFYIETKLDGERMQMH 282 L + KPMLA + V F E K DGER +H Sbjct 80134 PVLLESGHENLEDSCKLTPGIPLKPMLAHPTKAISEVLDRFDGVPFTCEYKYDGERA*IH 80313 Query 283 K-DGALYRYFSRNGYNYTDQFGESPQEGSLTPFIHNAFGTDVQACILDGEMMAYNPTTQT 341 + FSRN N ++++ + + ++ A V + ++D E +A++ + Sbjct 80314 QLSPGKVVIFSRNQENMSEKYPD------IVAAMNKAPKAGVSSFVIDSEAVAWDREKK* 80475 Query 342 FMQKGVKFDIKR---MVEDSGLQTCYSVFDVLMVNKKKLGRETLRKRYEILSSTFTPIQG 398 + V KR ED +Q C FD+L +N L R + +R +L +F I+G Sbjct 80476 ILPFQVLSTRKRKDVSAEDIKIQVCLFAFDLLYLNG*SLVRHSFAERRRMLYESFHEIEG 80655 Query 399 RIEIVQKTQAHTKKEVVDALNDAIDKREEGIMVK--HPLSIYKPDKRGEGWLKIKPEYVS 456 + +T +++ L++++ EG+MVK S Y+P KR WLK+K +Y+ Sbjct 80656 EFTFAKHQDGNTIEDI*MFLDESVAGNCEGLMVKTVDENSSYEPSKRSRNWLKVKKDYLD 80835 Query 457 GLMDELDVLIVGGYWGKGSRGGMMSHFLCAVAETPPPGDRPSVFHTLCRVGSGYTMKELY 516 G+ D LD+++VG Y GKG R G FL A + P + + T+ ++ +G++ +L Sbjct 80836 GVGDSLDLVVVGAYHGKGKRTGGFGGFLLACFD--PEREE---YQTISKIATGFSEDDL- 80997 Query 517 DLGLKLAKYWKPFHKKSPPSSILCGTE-KPEVYIEPQNSVIVQIKAAEIVPSDMYKT--- 572 K ++K + P S G KP+V++EP + ++K A++ S +Y Sbjct 80998 ---AKHTTFFKDYVISGPKSYYTFGEGVKPDVWLEPVQ--VWEVKTADLSISPVYTAAKG 81162 Query 573 ------GSTLRFPRIEKIRDDK 588 G +LRFPR +IRDDK Sbjct 81163 LVDPAKGISLRFPRFVRIRDDK 81228 > scaffold-423 Length=1446679 Score = 133 bits (335), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 129/490 (26%), Positives = 223/490 (46%), Gaps = 68/490 (14%) Frame = +1 Query 144 TIQQVNELLDLVASNNSGKKKDLVKKSLLQLITQSSALEQKWLIRMIIKDLKLGISQQTI 203 + + E+ + ++ KK D +K L+ E K++IRM+ L++G+++ ++ Sbjct 749233 VFKTLTEISKITGHSSMQKKIDKIK----FLLVSCRDNETKFIIRMLEGKLRIGLAEASV 749400 Query 204 FSIFHNDAVELHNVTT---DLE-------KVCRQLHDPSVGLSDISITLFSA-------- 245 + + +V N DLE +V +Q++ I L + Sbjct 749401 LTALAHASVLSRNSELKR*DLEGELS*AVEVVKQVYSEVPMYERIVPVLLESGHESLEDS 749580 Query 246 --------FKPMLA-AVADVERVEKDMKQQSFYIETKLDGERMQMH--KDGALYRYFSRN 294 KPMLA + V F E K DGER +H G + FSRN Sbjct 749581 CKLTPGIPLKPMLAHPTKAISEVLDRFDGVPFTCEYKYDGERA*IHLLSPGKVV-IFSRN 749757 Query 295 GYNYTDQFGESPQEGSLTPFIHNAFGTDVQACILDGEMMAYNPTTQTFMQKGVKFDIKR- 353 N ++++ + + ++ A DV + ++D E +A++ + + V KR Sbjct 749758 *ENMSEKYPD------IVAAMNKAPKADVSSFVIDSEAVAWDREKK*ILPF*VLSTRKRK 749919 Query 354 --MVEDSGLQTCYSVFDVLMVNKKKLGRETLRKRYEILSSTFTPIQGRIEIVQKTQAHTK 411 ED +Q C FD+L +N L R + +R +L +F I+G + +T Sbjct 749920 DVSAEDIKIQVCLFAFDLLYLNG*SLVRHSFAERRRMLYESFNEIEGEFTFAKHQDGNTI 750099 Query 412 KEVVDALNDAIDKREEGIMVK--HPLSIYKPDKRGEGWLKIKPEYVSGLMDELDVLIVGG 469 +++ L++++ EG+MVK S Y+P KR WLK+K +Y+ G+ D LD+++VG Sbjct 750100 EDI*MFLDESVAGNCEGLMVKTVDENSSYEPSKRSRNWLKVKKDYLDGVGDSLDLVVVGA 750279 Query 470 YWGKGSRGGMMSHFLCAVAETPPPGDRPSVFHTLCRVGSGYTMKELYDLGLKLAKYWKPF 529 Y GKG R G FL A + P + + T+ ++ +G++ +L K ++K Sbjct 750280 YHGKGKRTGGFGGFLLACFD--PEREE---YQTISKIATGFSEDDL----AKHTTFFKD- 750429 Query 530 HKKSPPSSILCGTE--KPEVYIEPQNSVIVQIKAAEIVPSDMYKT---------GSTLRF 578 H S P S E KP+V++EP + ++K A++ S +Y G +LRF Sbjct 750430 HVISDPKSYYTFGEGVKPDVWLEPVQ--VWEVKTADLSISPVYTAAKGLVDPAKGISLRF 750603 Query 579 PRIEKIRDDK 588 PR +IRDDK Sbjct 750604 PRFVRIRDDK 750633 > scaffold-26 Length=483268 Score = 32.3 bits (72), Expect = 1.5, Method: Compositional matrix adjust. Identities = 23/73 (32%), Positives = 35/73 (48%), Gaps = 12/73 (16%) Frame = +3 Query 841 TALELRFHGAKVV----SCLSEGVSHVIIGEDQRRVTDFKIFRRMLKKKFKILQESWVSD 896 T L HGA V+ S +S G++H I V RM + K +Q WV D Sbjct 196452 TLFMLLAHGANVILPF*SAVSSGITHEICDRPLEHV-------RMEPNR-KYVQPQWVFD 196607 Query 897 SVDKGELQEENQY 909 S+++G++ E+ Y Sbjct 196608 SINQGKILSEDLY 196646 Lambda K H a alpha 0.319 0.136 0.402 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 6261154020 Database: X5.fasta Posted date: Feb 18, 2021 11:04 PM Number of letters in database: 23,962,143 Number of sequences in database: 1,868 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 13 Window for multiple hits: 40