AatII	U65398	Type II	R	GACGTC	345	-	AAC44856.1	P70725	1498744	no	-
AbrI	X62690	Type II	R	CTCGAG	222	-	CAA44567.1	P26919	38661	no	-
AclI	HQ327695	Type II	R	AACGTT	323	-	ADO24175.1	E3VX94	308229523	no	-
Afa22MI	AB117613	Type II	R	CGATCG	259	-	BAC81825.1	Q762K1	34014718	no	-
AspBHII	AM406670	Type II	R	RGGWCCY	367	-	-	-	-	no	-
AvaII	BA000019	Type II	R	GGWCC	230	-	BAB72890.1	Q8YYB7	17130279	no	-
BamFI	FN597644	Type II	R	GGATCC	213	BAMF_3131	CBI44257.1	E1UQE5	307607886	no	-
BamHI	CP041693	Type II	R	GGATCC	213	FOG69_15995	QDP93514.1	-	-	no	-
BbrUII	CP000303	Type II	R	GTCGAC	695	Bbr_1120	ABE95798.1	C1J0P6	339479330	no	-
BceSII	AE017194	Type II	R	GGWCC	264	BCE_4604	AAS43505.1	Q72ZR4	42739579	no	-
BclI	CP025075	Type II	R	TGATCA	285	CWI35_18048	AUZ97187.1	E5LGB8	-	no	-
BfaIA	HQ446228	Type II	R	CTAG	205	-	ADQ20485.1	E5LG97	311977224	no	BfaI
BfaIB	HQ446228	Type II	R	CTAG	196	-	ADQ20486.1	E5LG98	311977225	no	BfaI
BglII	CP014840	Type II	R	AGATCT	223	A1D11_20260	AMR64577.1	-	100929147	no	-
BsaAI	HQ446231	Type II	R	YACGTR	260	-	ADQ20494.1	E5LGA6	311977236	no	-
Bsp98I	AJ245583	Type II	R	GGATCC	199	-	CAB52545.1	Q9RM91	5730143	no	-
BstFI	NEBC15050	Type II	R	AAGCTT	318	-	-	-	-	no	-
BstVI	L07643	Type II	R	CTCGAG	224	-	AAA22281.1	P43421	142619	no	-
BstZ1II	AY789018	Type II	R	AAGCTT	318	-	AAX14642.1	Q32WE8	60202513	no	-
BsuMIA	AL009126	Type II	R	CTCGAG	313	BSU_06090	CAB12428.1	O35025	-	no	BsuMI
BsuMIB	AL009126	Type II	R	CTCGAG	343	BSU_06100	CAB12429.1	O34885	-	no	BsuMI
BsuMIC	AL009126	Type II	R	CTCGAG	465	BSU_06110	CAB12430.1	O34303	-	no	BsuMI
CchI	CP000108	Type II	R	CTAG	197	Cag_1100	ABB28362.1	Q3ARL3	78171266	no	-
Cfr42I	EU262735	Type II	R	CCGCGG	216	-	ABY83632.1	B0LL99	166163614	no	-
Cfr9I	X17022	Type II	R	CCCGGG	330	-	CAA34888.1	Q60132	40465	no	-
ClaI	JF323047	Type II	R	ATCGAT	359	-	ADX97300.1	F1KC47	323650446	no	-
CphBI	CP001101	Type II	R	CCCGGG	257	Cphamn1_1063	ACE04003.1	B3EQH0	189495455	no	-
Csp231I	AY787793	Type II	R	AAGCTT	313	-	AAX19733.1	Q32WH3	60418613	no	-
Csp68KI	CP000806	Type II	R	GGWCC	233	cce_3474	ACB52822.1	B1WZS3	171699841	no	-
Csp68KII	CP000806	Type II	R	TTCGAA	219	cce_2281	ACB51631.1	B1WPR1	171698650	no	-
DdsI	CP001358	Type II	R	GGATCC	201	Ddes_2242	ACL50137.1	B8J492	-	no	-
Eco29kI	AJ001708	Type II	R	CCGCGG	214	-	CAA04943.1	Q46944	2660512	no	-
Eco47I	X82105	Type II	R	GGWCC	230	-	CAA57630.1	P50194	558572	no	-
Eco47IA	GQ231553	Type II	R	GGWCC	133	-	-	-	-	no	Eco47I
Eco47IB	GQ231553	Type II	R	GGWCC	119	-	-	-	-	no	Eco47I
Eco47II	X82105	Type II	R	GGNCC	239	-	CAA57628.1	P50195	558570	no	-
EcoO109I	AF157599	Type II	R	RGGNCCY	272	-	AAF06964.1	Q9RPJ3	6318607	no	-
EcoVIII	AF158026	Type II	R	AAGCTT	307	-	AAF71176.1	Q9JP70	7960014	no	-
FseI	JF323049	Type II	R	GGCCGGCC	219	-	ADX97304.1	F1KC51	323650452	no	-
FseI	JF323049	Type II	R	GGCCGGCC	219	-	ADX97304.1	F1KC51	323650452	no	-
FssI	CP002158	Type II	R	GGWCC	234	FSU_1381	ADL26476.1	C9RP75	302327275	no	-
HgiBI	X55137	Type II	R	GGWCC	274	-	CAA38928.1	P25257	43479	no	-
HgiCII	X55139	Type II	R	GGWCC	273	-	CAA38936.1	P25259	43465	no	-
HgiDII	X55141	Type II	R	GTCGAC	611	-	CAA38942.1	P25280	48774	no	-
HgiEI	X55142	Type II	R	GGWCC	274	-	CAA38945.1	P25260	43472	no	-
HincII	X54197	Type II	R	GTYRAC	258	-	CAA38115.1	E1B6R0	304942726	no	-
HindII	L42023	Type II	R	GTYRAC	258	HI_0512	AAC22170.1	P44413	1573497	no	-
HindIII	L42023	Type II	R	AAGCTT	300	HI_1393	AAC23040.1	P43870	1574230	no	-
LlaCI	HQ646604	Type II	R	AAGCTT	332	-	AEV59860.1	G9BNL2	359755276	no	-
LlaDI	AJ292520	Type II	R	AGTACT	251	-	CAC44540.1	Q93K18	15020665	no	-
MjaI	L77117	Type II	R	CTAG	222	MJ_0984	AAB98987.1	Q58391	1591646	no	-
MjaII	L77117	Type II	R	GGNCC	370	MJ_1449	AAB99461.1	Q58844	1592094	no	-
MluI	JF323053	Type II	R	ACGCGT	276	-	AEN71951.1	G3EST6	345106999	no	-
MthZI	X68367	Type II	R	CTAG	202	-	CAA48446.1	P29566	809724	no	-
NaeI	U09581	Type II	R	GCCGGC	317	-	AAC43324.1	P50187	775102	no	-
NgoAIII	AE004969	Type II	R	CCGCGG	213	-	-	-	-	no	-
NgoAIV	AE004969	Type II	R	GCCGGC	286	NGO_0874	AAW89575.1	Q5F8B2	59718170	no	-
NgoMIII	CP003909	Type II	R	CCGCGG	213	NGFG_02013	EEZ48556.1	-	268583880	no	-
NgoMIV	CP003909	Type II	R	GCCGGC	286	NGFG_00854	EEZ47687.1	-	268583011	no	-
NheI	AY462277	Type II	R	GCTAGC	328	-	AAR23810.1	Q6S994	38564763	no	-
NmeSI	AF123569	Type II	R	AGTACT	199	-	AAD39135.2	Q9XCX4	28212209	no	-
Nno22aORF54440P	CP006850	Type II	R	GTCGAC	307	NONO_c54450	AHH20225.1	W5TLJ5	576087835	yes	-
NspV	D14719	Type II	R	TTCGAA	220	-	BAA03538.1	P35677	442473	no	-
Pac25I	U88088	Type II	R	CCCGGG	333	-	AAD40333.1	Q9XAW7	5123479	no	-
PaeR7I	X03274	Type II	R	CTCGAG	246	-	CAA27026.1	P05104	45387	no	-
PciI	JX481785	Type II	R	ACATGT	257	-	AFV09321.1	K4LNB0	409034609	no	-
PmeII	CP027657	Type II	R	GGWCC	243	C7A17_16270	AVO54257.1	A0A2R3Q	-	no	-
PspPI	Y07554	Type II	R	GGNCC	32	-	-	-	-	no	-
PvuI	NEBC13909	Type II	R	CGATCG	260	-	-	-	-	no	-
R1.BsuMI	CP010052	Type II	R	CTCGAG	313	QU35_03530	AIY91901.1	-	728883506	no	-
R2.BsuMI	CP010052	Type II	R	CTCGAG	343	QU35_03535	AIY91902.1	-	728883507	no	-
R3.BsuMI	CP010052	Type II	R	CTCGAG	465	QU35_03540	AIY91903.1	-	728883508	no	-
RflFI	CC820644	Type II	R	GTCGAC	126	-	-	-	-	no	-
RrhJ1I	DJ340847	Type II	R	GCCGGC	308	-	-	-	-	no	-
RshI	CP000144	Type II	R	CGATCG	285	-	-	-	-	no	-
SacI	AF027867	Type II	R	GAGCTC	358	-	AAC97119.1	O31074	2605795	no	-
SalI	U01232	Type II	R	GTCGAC	315	-	AAA81886.1	Q53608	402237	no	-
Sau96I	X53096	Type II	R	GGNCC	261	-	CAA37259.1	P23736	46618	no	-
ScaI	NEBM97	Type II	R	AGTACT	227	-	-	-	-	no	-
SenpCI	AY079201	Type II	R	CCGCGG	214	-	AAL87018.1	Q8RLL0	19526585	no	-
SghWII	ABYA01000270	Type II	R	GCCGGC	401	-	-	-	-	no	-
Sgr13350I	AP009493	Type II	R	GAGCTC	397	SGR_3359	BAG20188.1	B1VM78	178465668	no	-
SinI	J03391	Type II	R	GGWCC	230	-	AAA27213.1	P09796	154351	no	-
SmaI	X16458	Type II	R	CCCGGG	247	-	CAA34478.1	P14229	47264	no	-
SnaBI	AY461517	Type II	R	TACGTA	223	-	AAS19439.1	Q6SA25	42541082	no	-
SonI	AE014300	Type II	R	ATCGAT	348	SO_A0003	AAN52942.1	Q8E8A6	24344699	no	-
SpeI	NEBC15359	Type II	R	ACTAGT	208	-	-	-	-	no	-
TdeIII	AE017226	Type II	R	GGNCC	258	TDE_0911	AAS11402.1	Q73P88	41816616	no	-
TliI	CP006670	Type II	R	CTCGAG	281	OCC_13281	EHR77447.1	H3ZRP1	374741014	no	-
TspMI	EF426476	Type II	R	CCCGGG	379	-	ABO38150.1	A4L311	133753294	no	-
XamI	U77781	Type II	R	GTCGAC	274	-	AAD13687.1	P96189	1688236	no	-
XcyI	M98768	Type II	R	CCCGGG	333	-	AAA27609.1	P30773	155372	no	-
XhoI	NEBC13645	Type II	R	CTCGAG	246	-	-	-	-	no	-
XmnI	NEBC13638	Type II	R	GAANNNNTTC	374	-	-	-	-	no	-
XorKI	AE013598	Type II	R	CGATCG	265	XOO0608	AAW73862.1	Q5H5A8	58424825	no	-