MAST - Motif Alignment and Search Tool
MAST version 4.3.0 (Release date: Sat Sep 26 01:51:56 PDT 2009)
For further information on how to interpret these results or to get
a copy of the MAST software please access http://meme.nbcr.net.
REFERENCE
If you use this program in your research, please cite:
Timothy L. Bailey and Michael Gribskov,
"Combining evidence using p-values: application to sequence homology
searches", Bioinformatics, 14(48-54), 1998.
DATABASE AND MOTIFS
DATABASE ./PF01812_seed.fasta (peptide)
Last updated on Sun May 12 01:13:38 2013
Database contains 24 sequences, 4372 residues
MOTIFS ./meme.txt (peptide)
MOTIF WIDTH BEST POSSIBLE MATCH
----- ----- -------------------
1 29 IDLIIVPGVAFDRNGNRLGYGKGYYDRFL
Random model letter frequencies (from non-redundant database):
A 0.073 C 0.018 D 0.052 E 0.062 F 0.040 G 0.069 H 0.022 I 0.056 K 0.058
L 0.092 M 0.023 N 0.046 P 0.051 Q 0.041 R 0.052 S 0.074 T 0.059 V 0.064
W 0.013 Y 0.033
SECTION I: HIGH-SCORING SEQUENCES
- Each of the following 24 sequences has E-value less than 10.
- The E-value of a sequence is the expected number of sequences
in a random database of the same size that would match the motifs as
well as the sequence does and is equal to the combined p-value of the
sequence times the number of sequences in the database.
- The combined p-value of a sequence measures the strength of the
match of the sequence to all the motifs and is calculated by
- finding the score of the single best match of each motif
to the sequence (best matches may overlap),
- calculating the sequence p-value of each score,
- forming the product of the p-values,
- taking the p-value of the product.
- The sequence p-value of a score is defined as the
probability of a random sequence of the same length containing
some match with as good or better a score.
- The score for the match of a position in a sequence to a motif
is computed by by summing the appropriate entry from each column of
the position-dependent scoring matrix that represents the motif.
- Sequences shorter than one or more of the motifs are skipped.
- The table is sorted by increasing E-value.
| Links | Sequence Name | Description | E-value | Length
|
|---|
|
| MTHFS_HUMAN/10-198
|
| 1.3e-23
| 189
|
|
| MTHFS_RABIT/10-198
|
| 1.5e-23
| 189
|
|
| YQGN_BACSU/2-179
|
| 1.6e-23
| 178
|
|
| Y858_HAEIN/6-187
|
| 2.7e-20
| 182
|
|
| Q9RTF0_DEIRA/10-175
|
| 5.3e-20
| 166
|
|
| O84655_CHLTR/8-178
|
| 9.8e-20
| 171
|
|
| Q9ZD82_RICPR/3-172
|
| 1e-19
| 170
|
|
| O67621_AQUAE/3-175
|
| 3.9e-19
| 173
|
|
| O68985_CHLTP/8-188
|
| 1.3e-18
| 181
|
|
| O83692_TREPA/4-207
|
| 2.3e-17
| 204
|
|
| P73038_SYNY3/3-179
|
| 2.3e-17
| 177
|
|
| O66124_STRMU/3-175
|
| 1.4e-16
| 173
|
|
| Q9Z5G2_MYCLE/6-186
|
| 1e-15
| 181
|
|
| O05575_MYCTU/6-186
|
| 1.2e-15
| 181
|
|
| Q9Z7E3_CHLPN/8-180
|
| 1.4e-15
| 173
|
|
| MTHFS_CAEEL/8-197
|
| 6.9e-15
| 190
|
|
| MTHFS_MYCPN/3-163
|
| 2.9e-14
| 161
|
|
| MTHFS_MYCGE/4-164
|
| 3.7e-14
| 161
|
|
| Q9RKA5_STRCO/12-197
|
| 5.1e-14
| 186
|
|
| O26306_METTH/134-322
|
| 2.9e-09
| 189
|
|
| Q9VN21_DROME/34-232
|
| 1.2e-07
| 199
|
|
| FTHC_YEAST/4-203
|
| 0.00036
| 200
|
|
| Q9YFU3_AERPE/7-205
|
| 0.0073
| 199
|
|
| Q9SRE0_ARATH/92-290
|
| 2.5
| 199
|
SECTION II: MOTIF DIAGRAMS
- The ordering and spacing of all non-overlapping motif occurrences
are shown for each high-scoring sequence listed in Section I.
- A motif occurrence is defined as a position in the sequence whose
match to the motif has POSITION p-value less than 0.0001.
- The POSITION p-value of a match is the probability of
a single random subsequence of the length of the motif
scoring at least as well as the observed match.
- For each sequence, all motif occurrences are shown unless there
are overlaps. In that case, a motif occurrence is shown only if its
p-value is less than the product of the p-values of the other
(lower-numbered) motif occurrences that it overlaps.
- The table also shows the E-value of each sequence.
- Spacers and motif occurences are indicated by
- Spacers and motif occurences are indicated by
- Spacers and motif occurences are indicated by
- occurrence of motif `n' with p-value less than 0.0001.
- Sequences longer than 1000 are not shown to scale and are indicated by thicker lines.
| Links | Name | Expect | Motifs
|
|---|
|
| MTHFS_HUMAN/10-198
| 1.3e-23
|
|
|
| MTHFS_RABIT/10-198
| 1.5e-23
|
|
|
| YQGN_BACSU/2-179
| 1.6e-23
|
|
|
| Y858_HAEIN/6-187
| 2.7e-20
|
|
|
| Q9RTF0_DEIRA/10-175
| 5.3e-20
|
|
|
| O84655_CHLTR/8-178
| 9.8e-20
|
|
|
| Q9ZD82_RICPR/3-172
| 1e-19
|
|
|
| O67621_AQUAE/3-175
| 3.9e-19
|
|
|
| O68985_CHLTP/8-188
| 1.3e-18
|
|
|
| O83692_TREPA/4-207
| 2.3e-17
|
|
|
| P73038_SYNY3/3-179
| 2.3e-17
|
|
|
| O66124_STRMU/3-175
| 1.4e-16
|
|
|
| Q9Z5G2_MYCLE/6-186
| 1e-15
|
|
|
| O05575_MYCTU/6-186
| 1.2e-15
|
|
|
| Q9Z7E3_CHLPN/8-180
| 1.4e-15
|
|
|
| MTHFS_CAEEL/8-197
| 6.9e-15
|
|
|
| MTHFS_MYCPN/3-163
| 2.9e-14
|
|
|
| MTHFS_MYCGE/4-164
| 3.7e-14
|
|
|
| Q9RKA5_STRCO/12-197
| 5.1e-14
|
|
|
| O26306_METTH/134-322
| 2.9e-09
|
|
|
| Q9VN21_DROME/34-232
| 1.2e-07
|
|
|
| FTHC_YEAST/4-203
| 0.00036
|
|
|
| Q9YFU3_AERPE/7-205
| 0.0073
|
|
|
| Q9SRE0_ARATH/92-290
| 2.5
|
|
| SCALE
|
| | |
| |
| |
| |
| |
| |
| |
| |
| 1 |
25 |
50 |
75 |
100 |
125 |
150 |
175 |
|
|---|
SECTION III: ANNOTATED SEQUENCES
- The positions and p-values of the non-overlapping motif occurrences
are shown above the actual sequence for each of the high-scoring
sequences from Section I.
- A motif occurrence is defined as a position in the sequence whose
match to the motif has POSITION p-value less than 0.0001 as
defined in Section II.
- For each sequence, the first line specifies the name of the sequence.
- The second (and possibly more) lines give a description of the
sequence.
- Following the description line(s) is a line giving the length,
combined p-value, and E-value of the sequence as defined in Section I.
- The next line reproduces the motif diagram from Section II.
- The entire sequence is printed on the following lines.
- Motif occurrences are indicated directly above their positions in the
sequence on lines showing
- the motif number of the occurrence,
- the position p-value of the occurrence,
- the best possible match to the motif, and
- columns whose match to the motif has a positive score (indicated
by a plus sign).
MTHFS_HUMAN/10-198
LENGTH = 189 COMBINED P-VALUE = 5.32e-25 E-VALUE = 1.3e-23
DIAGRAM: 119-[1]-41
[1]
3.3e-27
IDLIIVPGVAFDRNGNRLGYGKGYYDRFL
++++++++++++++++++++++++++ ++
76 FQSNHMDMVRIESPEEISLLPKTSWNIPQPGEGDVREEALSTGGLDLIFMPGLGFDKHGNRLGRGKGYYDAYLKR
MTHFS_RABIT/10-198
LENGTH = 189 COMBINED P-VALUE = 6.12e-25 E-VALUE = 1.5e-23
DIAGRAM: 119-[1]-41
[1]
3.8e-27
IDLIIVPGVAFDRNGNRLGYGKGYYDRFL
+++++++++++++++++++++ +++++++
76 LQSNHMDMVKLASADEISSLPKTSWNIHQPSESDTREEALATGGLDLIFMPGLGFDRNGNRLGRGRGYYDTYLQR
YQGN_BACSU/2-179
LENGTH = 178 COMBINED P-VALUE = 6.55e-25 E-VALUE = 1.6e-23
DIAGRAM: 114-[1]-35
[1]
4.4e-27
IDLIIVPGVAFDRNGNRLGYGKGYYDRFL
++++++++++++ ++++++++++++++++
76 PDTKKMQFRTYQTDDQLETVYAGLLEPVIEKTKEVNPSQIDLMIVPGVCFDVNGFRVGFGGGYYDRYLSEYEGKT
Y858_HAEIN/6-187
LENGTH = 182 COMBINED P-VALUE = 1.14e-21 E-VALUE = 2.7e-20
DIAGRAM: 115-[1]-38
[1]
7.4e-24
IDLIIVPGVAFDRNGNRLGYGKGYYDRFL
+++ + + +++++++++++++++++++ +
76 PFTKHYLLFLRYLPDTPMKQNQFGIWEPKLNVQNVLPLNELDILFTPLVAFDKKGNRLGMGGGFYDRTLQNWQNK
Q9RTF0_DEIRA/10-175
LENGTH = 166 COMBINED P-VALUE = 2.21e-21 E-VALUE = 5.3e-20
DIAGRAM: 102-[1]-35
[1]
1.6e-23
IDLIIVPGVAFDRNGNRLGYGKGYYDRFL
+++++++++++++++ +++++++++++++
76 PYATATEVSRAGYLQPPTTAPQLPLGSVDAVLLPGLAFDRHGTRLGYGGGFYDRLLLGYAGLLVGVVWSALLLPE
O84655_CHLTR/8-178
LENGTH = 171 COMBINED P-VALUE = 4.07e-21 E-VALUE = 9.8e-20
DIAGRAM: 106-[1]-36
[1]
2.8e-23
IDLIIVPGVAFDRNGNRLGYGKGYYDRFL
+ + +++++ ++ + ++++++++++++++
76 VPIALPFTKIESLYSPKDLNRIEGEEIEAQQIAAALIPAIVFDQNKFRLGYGGGYYDRFLSKYPYIWTIGVGFKE
Q9ZD82_RICPR/3-172
LENGTH = 170 COMBINED P-VALUE = 4.37e-21 E-VALUE = 1e-19
DIAGRAM: 105-[1]-36
[1]
3.1e-23
IDLIIVPGVAFDRNGNRLGYGKGYYDRFL
++++++++++ + ++++++++++++ ++
76 EIRYCAYNFNDALVLGKCKTYEPINNNFIIPELVIIPGLAFSKDGYRLGYGKGYFDQYLNNHQNIFTVGVCLKEQ
O67621_AQUAE/3-175
LENGTH = 173 COMBINED P-VALUE = 1.62e-20 E-VALUE = 3.9e-19
DIAGRAM: 109-[1]-35
[1]
1.1e-22
IDLIIVPGVAFDRNGNRLGYGKGYYDRFL
++ + +++++++ +++++++++++++++
76 NEISLYRVHSPACLGVGAFGIMEPVEGERVNPEDVDFIAVPGVAFDLEGYRLGFGKGYYDRLLKRVKGLKVGVAY
O68985_CHLTP/8-188
LENGTH = 181 COMBINED P-VALUE = 5.53e-20 E-VALUE = 1.3e-18
DIAGRAM: 112-[1]-40
[1]
3.6e-22
IDLIIVPGVAFDRNGNRLGYGKGYYDRFL
+++++++ ++++++++++++++++++++
76 IEQGIMKTSIYHSAQKFRISVSAPPTPEPLVLSGEERFEVVFVPLTGFDRRGGRIGFGKGWYDRFFSRLSGHGIH
O83692_TREPA/4-207
LENGTH = 204 COMBINED P-VALUE = 9.63e-19 E-VALUE = 2.3e-17
DIAGRAM: 133-[1]-42
[1]
5.5e-21
IDLIIVPGVAFDRNGNR
+++++++++ ++ +
76 NDLHFHAVTYACTTRPSVSCFTTLCPRTRGIREPDAHSPRLYPPHPSPNTPAQRTLALPLLIVVPALAFSTNGAR
LGYGKGYYDRFL
++++++++++++
151 LGRGGGHYDRFLARIAATIPAGSYYTLGLCFDCQIMAVIPQEAHDQSVHAVLTE
P73038_SYNY3/3-179
LENGTH = 177 COMBINED P-VALUE = 9.73e-19 E-VALUE = 2.3e-17
DIAGRAM: 110-[1]-38
[1]
6.5e-21
IDLIIVPGVAFDRNGNRLGYGKGYYDRFL
+++ +++ ++ ++++++++++++++++++
76 KSLVWHPWQWGQPLTTGAYGIKVPADQTEIWSPNTVDLLLVPCLAGDRQGYRLGYGGGYYDRLFADPLWRAVPKV
O66124_STRMU/3-175
LENGTH = 173 COMBINED P-VALUE = 5.74e-18 E-VALUE = 1.4e-16
DIAGRAM: 110-[1]-34
[1]
4.0e-20
IDLIIVPGVAFDRNGNRLGYGKGYYDRFL
++++ ++++++ + ++++++++++++ ++
76 PQRKMIFVVYDEADLQITKFGLKEPRSEEALEKSAIDLIHVPGLAFNNEGYRIGFGAGYYDQYLADFQGDTVSTI
Q9Z5G2_MYCLE/6-186
LENGTH = 181 COMBINED P-VALUE = 4.35e-17 E-VALUE = 1e-15
DIAGRAM: 115-[1]-37
[1]
2.8e-19
IDLIIVPGVAFDRNGNRLGYGKGYYDRFL
+++++++++ + ++ +++++++++++ +
76 DDEIPLPLQWGEYRPGGLTSGPWGLLEPPELRLPESALAEANLVLVPALAVDHHGVRLGRGGGFYDRSLAGRDPH
O05575_MYCTU/6-186
LENGTH = 181 COMBINED P-VALUE = 4.83e-17 E-VALUE = 1.2e-15
DIAGRAM: 115-[1]-37
[1]
3.2e-19
IDLIIVPGVAFDRNGNRLGYGKGYYDRFL
++++++++ ++++ +++++ +++++ +
76 GGDLPLPLRWGEYRAGGLARARWGLLEPPEPWLPEAALAQASLVLVPALAVDRQGVRLGRGRGFYDRSLRCRDPH
Q9Z7E3_CHLPN/8-180
LENGTH = 173 COMBINED P-VALUE = 5.64e-17 E-VALUE = 1.4e-15
DIAGRAM: 106-[1]-38
[1]
3.9e-19
IDLIIVPGVAFDRNGNRLGYGKGYYDRFL
+ +++++++++ ++++++++++++++ +
76 YPVLIPSIDDLISVVHPKDPFSKQTPISSDKITHVLVPGLAFDQQGYRLGYGHGFYDRWLAQHPYPSIRTIGIGY
MTHFS_CAEEL/8-197
LENGTH = 190 COMBINED P-VALUE = 2.88e-16 E-VALUE = 6.9e-15
DIAGRAM: 119-[1]-42
[1]
1.8e-18
IDLIIVPGVAFDRNGNRLGYGKGYYDRFL
+++++ +++++ + + +++++++++++
76 KGSTAMDMVRVPDQTAFDNLPSTLWGIRQPEPKWKWQSYHETGPLDLILAPGVAFSPYGLRCGHGKGYYDRFFST
MTHFS_MYCPN/3-163
LENGTH = 161 COMBINED P-VALUE = 1.21e-15 E-VALUE = 2.9e-14
DIAGRAM: 96-[1]-36
[1]
9.1e-18
IDLIIVPGVAFDRNGNRLGYGKGYYDRFL
++ +++ +++ + +++++++++++++++
76 VISDTEIIFIDQETNTFEPNQIDCFLIPLVGFNKDNYRLGFGKGYYDRYLMQLTRQQPKIGIAYSFQKGDFLADP
MTHFS_MYCGE/4-164
LENGTH = 161 COMBINED P-VALUE = 1.54e-15 E-VALUE = 3.7e-14
DIAGRAM: 96-[1]-36
[1]
1.2e-17
IDLIIVPGVAFDRNGNRLGYGKGYYDRFL
++ +++ +++ + +++++++++++++++
76 IVDQFNMKFIDQNNNEINPNDIDCFFIPLLAFNKANHRIGFGKGYYDRYLSLTSKKQLKIGIAYDFQYAEFTNDP
Q9RKA5_STRCO/12-197
LENGTH = 186 COMBINED P-VALUE = 2.12e-15 E-VALUE = 5.1e-14
DIAGRAM: 117-[1]-40
[1]
1.3e-17
IDLIIVPGVAFDRNGNRLGYGKGYYDRFL
+++++++++ + ++ +++++++ +++ +
76 PDNDLDWGEYTGEGSLARVRHGGRMELFEPAGERLGPEAVTRADVVLLPGVAVDGRGLRLGRGGGSYDRVLARLE
O26306_METTH/134-322
LENGTH = 189 COMBINED P-VALUE = 1.21e-10 E-VALUE = 2.9e-09
DIAGRAM: 11-[1]-77-[1]-43
[1]
6.7e-05
IDLIIVPGVAFDRNGNRLGYGKGYYDRFL
+++ + ++ +++ + + +
1 DGGERLVEYHIWDLLVEKGVSKRPHGRIPDFKGSFDAAKRLSRTVEWERAGVVFCSPDSAQSPVRRLVLEAGKDL
[1]
7.5e-13
IDLIIVPGVAFDRNGNRLGYGKGYYDRFL
+++++ ++ + ++++++ ++++ ++
76 IMPTPKIKDGYLLIGGDVPDAEAASTIGGAYMYGSPIREFPQVDLVVEGSVAVDLQGNRLGKGGGYGDREISELR
Q9VN21_DROME/34-232
LENGTH = 199 COMBINED P-VALUE = 5.19e-09 E-VALUE = 1.2e-07
DIAGRAM: 124-[1]-46
[1]
3.0e-11
IDLIIVPGVAFDRNGNRLGYGKGYYD
++++++ + + +++++++ ++ +
76 YLPSTRESSALCLKVDVLADATEEQKKEALRVQDIQKFRSEIGLDSGLKLDIVVIGSVVVSREGYRIGRGNGFAD
RFL
151 LDIGLLIELGAITPKTIIVTIVHDMQVVDSLPPNLFQKYDTPVDIIATP
FTHC_YEAST/4-203
LENGTH = 200 COMBINED P-VALUE = 1.48e-05 E-VALUE = 0.00036
DIAGRAM: 129-[1]-42
[1]
8.6e-08
IDLIIVPGVAFDRNGNRLGYG
+ + +++ +++++
76 THTSESKHFKLREDHHPHLIFHRMSSLKMVRDLKPQGPYQLKEPEPHIEESDILDVVLVPGVAFDIKTGARMGHG
KGYYDRFL
+++++ ++
151 AGYYDDFFQRYKILHEGQKPLLVGLCLMEQVASPIPLEKHDYSMDCIVCG
Q9YFU3_AERPE/7-205
LENGTH = 199 COMBINED P-VALUE = 3.04e-04 E-VALUE = 0.0073
DIAGRAM: 126-[1]-44
[1]
1.8e-06
IDLIIVPGVAFDRNGNRLGYGKGY
++ ++ ++ + + +++ + ++
76 MPTPRLRSGFLLLDPSTIPRNLYRVGSTIKGAFRLARPLASIQDLVQHVEKIDFVVEGSVAVNRWGERLGKGEGY
YDRFL
+ +
151 GDLEFAILVEAGILDREVAIATTVHDLQVLPHRLPQEEHDVSVDIISTP
Q9SRE0_ARATH/92-290
LENGTH = 199 COMBINED P-VALUE = 1.05e-01 E-VALUE = 2.5
DIAGRAM: 199
Debugging Information
CPU: kodomo.fbb.msu.ru
Time 0.004000 secs.
mast ./meme.txt -d ./PF01812_seed.fasta -ev 10.000000 -mt 0.000100
Button Help
Links to Entrez database at NCBI
Links to sequence scores (section I)
Links to motif diagrams (section II)
Links to sequence/motif annotated alignments (section III)
This information