ID   YXIM_BACSU              Reviewed;         382 AA.
AC   P42304;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 2.
DT   13-NOV-2013, entry version 89.
DE   RecName: Full=Uncharacterized esterase YxiM;
DE            EC=3.1.-.-;
DE   Flags: Precursor;
GN   Name=yxiM; OrderedLocusNames=BSU39120; ORFNames=SS8D;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / BGSC1A1;
RX   PubMed=8969509;
RA   Yoshida K., Shindo K., Sano H., Seki S., Fujimura M., Yanai N.,
RA   Miwa Y., Fujita Y.;
RT   "Sequencing of a 65 kb region of the Bacillus subtilis genome
RT   containing the lic and cel loci, and creation of a 177 kb contig
RT   covering the gnt-sacXY region.";
RL   Microbiology 142:3113-3123(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA   Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA   Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA   Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA   Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA   Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA   Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA   Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA   Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA   Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA   Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA   Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA   Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA   Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA   Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA   Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA   Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA   Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA   Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA   Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA   Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA   Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION TO 83.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G.,
RA   Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus
RT   subtilis 168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 17-382.
RG   Northeast structural genomics consortium (NESG);
RT   "X-ray structure of the hypothetical protein YXIM_BACSU from Bacillus
RT   subtilis.";
RL   Submitted (DEC-2006) to the PDB data bank.
CC   -!- SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family.
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DR   EMBL; D83026; BAA11692.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB15948.2; -; Genomic_DNA.
DR   PIR; A70078; A70078.
DR   RefSeq; NP_391791.2; NC_000964.3.
DR   PDB; 2O14; X-ray; 2.10 A; A=17-382.
DR   PDBsum; 2O14; -.
DR   ProteinModelPortal; P42304; -.
DR   SMR; P42304; 29-382.
DR   STRING; 224308.BSU39120; -.
DR   PaxDb; P42304; -.
DR   EnsemblBacteria; CAB15948; CAB15948; BSU39120.
DR   GeneID; 937485; -.
DR   KEGG; bsu:BSU39120; -.
DR   PATRIC; 18979886; VBIBacSub10457_4105.
DR   GenoList; BSU39120; -.
DR   eggNOG; COG2755; -.
DR   HOGENOM; HOG000087899; -.
DR   OrthoDB; EOG689HPV; -.
DR   ProtClustDB; CLSK626751; -.
DR   BioCyc; BSUB:BSU39120-MONOMER; -.
DR   EvolutionaryTrace; P42304; -.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1110; -; 1.
DR   InterPro; IPR008979; Galactose-bd-like.
DR   InterPro; IPR013831; SGNH_hydro-type_esterase_dom.
DR   SUPFAM; SSF49785; SSF49785; 1.
DR   PROSITE; PS01098; LIPASE_GDSL_SER; FALSE_NEG.
PE   1: Evidence at protein level;
KW   3D-structure; Complete proteome; Hydrolase; Reference proteome;
KW   Signal.
FT   SIGNAL        1     25       Potential.
FT   CHAIN        26    382       Uncharacterized esterase YxiM.
FT                                /FTId=PRO_0000013744.
FT   ACT_SITE    186    186       Nucleophile (By similarity).
FT   ACT_SITE    354    354       By similarity.
FT   ACT_SITE    357    357       By similarity.
FT   CONFLICT     83     83       Q -> E (in Ref. 1; BAA11692).
FT   STRAND       30     38
FT   STRAND       44     47
FT   TURN         55     57
FT   STRAND       58     62
FT   HELIX        64     66
FT   STRAND       67     71
FT   STRAND       73     75
FT   HELIX        76     79
FT   STRAND       80     86
FT   STRAND       95     98
FT   STRAND      101    112
FT   STRAND      114    120
FT   STRAND      123    131
FT   STRAND      135    142
FT   STRAND      144    155
FT   STRAND      161    173
FT   STRAND      179    184
FT   TURN        186    188
FT   TURN        194    196
FT   HELIX       202    205
FT   HELIX       206    208
FT   TURN        212    214
FT   STRAND      216    219
FT   HELIX       227    232
FT   HELIX       235    240
FT   STRAND      248    252
FT   HELIX       256    258
FT   HELIX       260    262
FT   HELIX       266    281
FT   TURN        282    284
FT   STRAND      286    290
FT   HELIX       313    322
FT   STRAND      326    328
FT   HELIX       330    341
FT   HELIX       343    347
FT   STRAND      355    359
FT   HELIX       360    376
SQ   SEQUENCE   382 AA;  41836 MW;  B46ADC4054B3C97D CRC64;
     MKKWMAAVFV MMLMLCFGGI ENVKAAEPKV YQFDFGSGSM EPGYIGVRAS DRYDRSKGYG
     FQTPENMRDV AASGAGVKSD AVQFLAYGTK SNNTFNVDLP NGLYEVKVTL GNTARASVAA
     EGVFQVINMT GDGAEDTFQI PVTDGQLNLL VTEGKAGTAF TLSALKIKKL SDQPVTNRTI
     YVGGDSTVCN YYPLNSSKQA GWGQMLPHYI DKHTFQVRNM ASGGQIARGF RNDGQLEAIL
     KYIKPGDYFM LQLGINDTNP KHKESEAEFK EVMRDMIRQV KAKGADVILS TPQGRATDFT
     SEGIHSSVNR WYRASILALA EEEKTYLIDL NVLSSAYFTS IGPERTLGLY MDGDTLHPNR
     AGADALARLA VQELKRQGIA GF
//