Для поиска выбрала β-тубулин, актин (белки цитоскелета) и гистон H3 (белок нуклеосомы). Все они впервые появляются у эукариот и несут базовые функции, поэтому должны быть консервативны и встречаться у подавляющего большинства эукариот. Использовала белки Saccharomyces cerevisiae, так как группа Aphelida родственна грибам.
Представлена только часть выдачи, поскольку по ней очевидно наличие белка (e-value - машинный ноль, выравнялась почти вся последовательность)
Query= sp|P02557|TBB_YEAST Tubulin beta chain OS=Saccharomyces cerevisiae
(strain ATCC 204508 / S288c) OX=559292 GN=TUB2 PE=1 SV=2
Length=457
Score E
Sequences producing significant alignments: (Bits) Value
unplaced-665 680 0.0
scaffold-26 631 0.0
scaffold-57 343 3e-105
unplaced-5 343 3e-105
scaffold-423 143 5e-42
scaffold-693 28.5 7.8
scaffold-17 28.5 8.0
> unplaced-665
Length=20473
Score = 680 bits (1754), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 339/451 (75%), Positives = 386/451 (86%), Gaps = 22/451 (5%)
Frame = -2
Query 1 MREIIHISTGQCGNQIGAAFWETICGEHGLDFNGTYHGHDDIQKERLN------------ 48
MREI+H+ TG CGN IGA FWE I EHG+D NG Y G D+Q ER+N
Sbjct 7236 MREIVHL*TG*CGN*IGAKFWEVISDEHGIDPNGRYEGDSDLQLERINGEFLNVLFCA** 7057
Query 49 ----------VYFNEASSGKWVPRSINVDLEPGTIDAVRNSAIGNLFRPDNYIFGQSSAG 98
VYFNEAS GK+VPR++ VDLEPGT+D+VR GNLFRPDN+IFGQS AG
Sbjct 7056 AFCITLLLIVVYFNEASGGKYVPRAVLVDLEPGTMDSVRAGPYGNLFRPDNFIFGQSGAG 6877
Query 99 NVWAKGHYTEGAELVDSVMDVIRREAEGCDSLQGFQIthslgggtgsgmgtllisKIREE 158
N WAKGHYTEGAELVDSV+DV+R+EAEGCD LQGFQITHSLGGGTG+GMGTLLISKIREE
Sbjct 6876 NNWAKGHYTEGAELVDSVLDVVRKEAEGCDCLQGFQITHSLGGGTGAGMGTLLISKIREE 6697
Query 159 FPDRMMATFSVLPSPKTSDTVVEPYNATLSVHQLVEHSDETFCIDNEALYDICQRTLKLN 218
+PDRMM TFSV+PSPK SDTVVEPYNATLSVHQLVE+SDETFCIDNEALYDIC RTLKL
Sbjct 6696 YPDRMMCTFSVVPSPKVSDTVVEPYNATLSVHQLVENSDETFCIDNEALYDICFRTLKLT 6517
Query 219 QPSYGDLNNLVSSVMSGVTTSLRYPGQLNSDLRKLAVNLVPFPRLHFFMVGYAPLTAIGS 278
P+YGDLN+LVS+VMSGVTTS+R+PGQLN+DLRKLAVN+VPFPRLHFFMVG+APLT+ GS
Sbjct 6516 TPTYGDLNHLVSAVMSGVTTSIRFPGQLNADLRKLAVNMVPFPRLHFFMVGFAPLTSRGS 6337
Query 279 QSFRSLTVPELTQQMFDAKNMMAAADPRNGRYLTVAAFFRGKVSVKEVEDEMHKVQSKNS 338
Q +R+L+V ELT QMFDAKNMMAA+DPR+GRYL VAA FRGK+S+KEV+++M VQ+KNS
Sbjct 6336 QQYRALSVAELTTQMFDAKNMMAASDPRHGRYLAVAAIFRGKMSMKEVDEQMLSVQTKNS 6157
Query 339 DYFVEWIPNNVQTAVCSVAPQGLDMAATFIANSTSIQELFKRVGDQFSAMFKRKAFLHWY 398
YFVEWIPNNV+TAVC + P+GL M+ATFI NST+IQELFKR+ DQFS MFKRKAFLHWY
Sbjct 6156 SYFVEWIPNNVKTAVCDIPPKGLKMSATFIGNSTAIQELFKRISDQFSVMFKRKAFLHWY 5977
Query 399 TSEGMDELEFSEAESNMNDLVSEYQQYQEAT 429
T EGMDE+EF+EAESNMNDLVSEYQQYQ+AT
Sbjct 5976 TGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 5884
> scaffold-26
Length=483268
Score = 631 bits (1628), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 308/381 (81%), Positives = 351/381 (92%), Gaps = 0/381 (0%)
Frame = -3
Query 49 VYFNEASSGKWVPRSINVDLEPGTIDAVRNSAIGNLFRPDNYIFGQSSAGNVWAKGHYTE 108
VY+NEAS GK+VPR++ VDLEPGT+D+VR GNLFRPDN+IFGQS AGN WAKGHYTE
Sbjct 109793 VYYNEASGGKYVPRAVLVDLEPGTMDSVRAGPYGNLFRPDNFIFGQSGAGNNWAKGHYTE 109614
Query 109 GAELVDSVMDVIRREAEGCDSLQGFQIthslgggtgsgmgtllisKIREEFPDRMMATFS 168
GAELVDSV+DV+R+EAEGCD LQGFQITHSLGGGTG+GMGTLLISKIREE+PDRMM TFS
Sbjct 109613 GAELVDSVLDVVRKEAEGCDCLQGFQITHSLGGGTGAGMGTLLISKIREEYPDRMMCTFS 109434
Query 169 VLPSPKTSDTVVEPYNATLSVHQLVEHSDETFCIDNEALYDICQRTLKLNQPSYGDLNNL 228
V+PSPK SDTVVEPYNATLSVHQLVE+SDETFCIDNEALYDIC RTLKL P+YGDLN+L
Sbjct 109433 VVPSPKVSDTVVEPYNATLSVHQLVENSDETFCIDNEALYDICFRTLKLTTPTYGDLNHL 109254
Query 229 VSSVMSGVTTSLRYPGQLNSDLRKLAVNLVPFPRLHFFMVGYAPLTAIGSQSFRSLTVPE 288
VS+VMSGVTTS+R+PGQLN+DLRKLAVN+VPFPRLHFFMVG+APLT+ GSQ +R+L+V E
Sbjct 109253 VSAVMSGVTTSIRFPGQLNADLRKLAVNMVPFPRLHFFMVGFAPLTSRGSQQYRALSVAE 109074
Query 289 LTQQMFDAKNMMAAADPRNGRYLTVAAFFRGKVSVKEVEDEMHKVQSKNSDYFVEWIPNN 348
LT QMFDAKNMMAA+DPR+GRYL VAA FRGK+S+KEV+++M VQ+KNS YFVEWIPNN
Sbjct 109073 LTTQMFDAKNMMAASDPRHGRYLAVAAIFRGKMSMKEVDEQMLSVQTKNSSYFVEWIPNN 108894
Query 349 VQTAVCSVAPQGLDMAATFIANSTSIQELFKRVGDQFSAMFKRKAFLHWYTSEGMDELEF 408
V+TAVC + P+GL M+ATFI NST+IQELFKR+ DQFS MFKRKAFLHWYT EGMDE+EF
Sbjct 108893 VKTAVCDIPPKGLKMSATFIGNSTAIQELFKRISDQFSVMFKRKAFLHWYTGEGMDEMEF 108714
Query 409 SEAESNMNDLVSEYQQYQEAT 429
+EAESNMNDLVSEYQQYQ+AT
Sbjct 108713 TEAESNMNDLVSEYQQYQDAT 108651
Ситуация аналогична предыдущей. Ген актина в геноме есть
Query= sp|P60010|ACT_YEAST Actin OS=Saccharomyces cerevisiae (strain ATCC
204508 / S288c) OX=559292 GN=ACT1 PE=1 SV=1
Length=375
Score E
Sequences producing significant alignments: (Bits) Value
scaffold-17 721 0.0
scaffold-444 721 0.0
scaffold-514 717 0.0
scaffold-693 376 6e-118
scaffold-170 374 2e-117
scaffold-96 356 4e-111
scaffold-423 356 4e-111
unplaced-1003 215 2e-70
unplaced-948 215 2e-70
unplaced-987 234 1e-68
unplaced-1039 191 4e-61
unplaced-925 191 4e-61
scaffold-277 188 6e-53
scaffold-199 180 4e-50
scaffold-105 62.8 1e-10
scaffold-115 45.4 4e-05
scaffold-361 45.1 5e-05
scaffold-157 30.4 1.7
scaffold-287 29.3 4.1
> scaffold-17
Length=2125590
Score = 721 bits (1861), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 333/375 (89%), Positives = 363/375 (97%), Gaps = 0/375 (0%)
Frame = -3
Query 1 MDSEVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGIMVGMGQKDSYVGDEAQS 60
M+ EVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQG+MVGMGQKDSYVGDEAQS
Sbjct 893374 MEDEVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQS 893195
Query 61 KRGILTLRYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPMNPKSNREKMT 120
KRGILTL+YPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVL TEAP+NPK+NREKMT
Sbjct 893194 KRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLCTEAPLNPKANREKMT 893015
Query 121 QIMFETFNVPAFYVSIQAVLSLYSSGRTTGIVLDSGDGVTHVVPIYAGFSLPHAILRIDL 180
QIMFETFNVPAFYV+IQAVLSLY+SGRTTGIVLDSGDGV+H VPIY G++LPHAILR+DL
Sbjct 893014 QIMFETFNVPAFYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDL 892835
Query 181 AGRDLTDYLMKILSERGYSFSTTAEREIVRDIKEKLCYVALDFEQEMQTAAQSSSIEKSY 240
AGRDLTDYLMKIL+ERGYSF+TTAEREIVRDIKEKLCYVALDFE EMQTA+ SS++EKSY
Sbjct 892834 AGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFE*EMQTASTSSALEKSY 892655
Query 241 ELPDGQVITIGNERFRAPEALFHPSVLGLESAGIDQTTYNSIMKCDVDVRKELYGNIVMS 300
ELPDGQVITIGNERFR PEALF PS LG+E+AG+ +TTYNSIMKCDVD+RK+LY NIV+S
Sbjct 892654 ELPDGQVITIGNERFRCPEALFQPSFLGMEAAGVHETTYNSIMKCDVDIRKDLYANIVLS 892475
Query 301 GGTTMFPGIAERMQKEITALAPSSMKVKIIAPPERKYSVWIGGSILASLTTFQQMWISKQ 360
GGTTM+PGIA+R+QKEITALAPSSMK+K++APPERKYSVWIGGSILASL+TFQQMWISKQ
Sbjct 892474 GGTTMYPGIADRVQKEITALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWISKQ 892295
Query 361 EYDESGPSIVHHKCF 375
EYDESGPSIVH KCF
Sbjct 892294 EYDESGPSIVHRKCF 892250
Score = 67.0 bits (162), Expect = 6e-12, Method: Compositional matrix adjust.
Identities = 56/239 (23%), Positives = 106/239 (44%), Gaps = 24/239 (10%)
Frame = +1
Query 3 SEVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGIMVGMGQKDSYVGDEAQSKR 62
S+ + +VID GS +AG+A D P+ VF ++V + + G D Y G +
Sbjct 1426510 SQESPIVIDFGSWQLRAGYADTD-PQLVFDNLVSKTS*GIHIGGGD*CD-YSGARSA--- 1426674
Query 63 GILTLRYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPMNPKSNREK 118
+ ++ + ++E H F N L+ E+ P++ TE N R
Sbjct 1426675 ------FDFS*SLIVHPPNLELTLDHVFANLLKAQREKTLA*RPIVFTETLCNLN*CRRI 1426836
Query 119 MTQIMFETFNVPAFYVSIQAVLSLYSSGRTTG---------IVLDSGDGVTHVVPIYAGF 169
++++MFE ++V + A+ S Y +G +G ++L SG +H++P+ G
Sbjct 1426837 VSELMFECYSVGRLAYGVDALFSFYHNGGFSGDDKKKKRNALILSSGHNASHIIPVIDGG 1427016
Query 170 SLPHAILRIDLAGRDLTDYLMKILSERGYSFSTTAEREIVRDIKEKLCYVALDFEQEMQ 228
R+D+ GR T+++ K L + + ++ + C VA D++ +M
Sbjct 1427017 VDYSHCKRLDIGGRSSTEFMQKHLLVKYPELNQKLSLMTTD*LRVEFCRVASDYKGDMN 1427193
Score = 58.2 bits (139), Expect = 4e-09, Method: Compositional matrix adjust.
Identities = 35/133 (26%), Positives = 71/133 (53%), Gaps = 7/133 (5%)
Frame = +1
Query 248 ITIGNERFRAPEALFHPSVLGLESAGIDQ--TTYNSIMKCDVDVRKELYGNIVMSGGTTM 305
I + ++R++ PE LF P ++G ESAG+ + +T + + D+ + ++ N+ ++GG TM
Sbjct 1428313 IKLSSDRYKVPEILFQPHLMGSESAGLSEMCSTLAKVYESDLS-KLQMMSNVFVTGGNTM 1428489
Query 306 FPGIAERMQKEITALAPSSMKVKIIAPPERKYSVWIGGS--ILASLTTFQ-QMWISKQEY 362
+PG R+ ++ + VK+ + W G S L L+ +++I+++ Y
Sbjct 1428490 YPGFD*RLFNDLR*NVLCDVPVKVYRAQDSILDAWRGASKWALDHLSNKN*KVFITRK*Y 1428669
Query 363 DESGPSIVH-HKC 374
+E G + H+C
Sbjct 1428670 EECGADYLQEHQC 1428708
> scaffold-444
Length=851481
Score = 721 bits (1861), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 333/375 (89%), Positives = 363/375 (97%), Gaps = 0/375 (0%)
Frame = -2
Query 1 MDSEVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGIMVGMGQKDSYVGDEAQS 60
M+ EVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQG+MVGMGQKDSYVGDEAQS
Sbjct 165923 MEDEVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQS 165744
Query 61 KRGILTLRYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPMNPKSNREKMT 120
KRGILTL+YPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVL TEAP+NPK+NREKMT
Sbjct 165743 KRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLCTEAPLNPKANREKMT 165564
Query 121 QIMFETFNVPAFYVSIQAVLSLYSSGRTTGIVLDSGDGVTHVVPIYAGFSLPHAILRIDL 180
QIMFETFNVPAFYV+IQAVLSLY+SGRTTGIVLDSGDGV+H VPIY G++LPHAILR+DL
Sbjct 165563 QIMFETFNVPAFYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDL 165384
Query 181 AGRDLTDYLMKILSERGYSFSTTAEREIVRDIKEKLCYVALDFEQEMQTAAQSSSIEKSY 240
AGRDLTDYLMKIL+ERGYSF+TTAEREIVRDIKEKLCYVALDFE EMQTA+ SS++EKSY
Sbjct 165383 AGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFE*EMQTASTSSALEKSY 165204
Query 241 ELPDGQVITIGNERFRAPEALFHPSVLGLESAGIDQTTYNSIMKCDVDVRKELYGNIVMS 300
ELPDGQVITIGNERFR PEALF PS LG+E+AG+ +TTYNSIMKCDVD+RK+LY NIV+S
Sbjct 165203 ELPDGQVITIGNERFRCPEALFQPSFLGMEAAGVHETTYNSIMKCDVDIRKDLYANIVLS 165024
Query 301 GGTTMFPGIAERMQKEITALAPSSMKVKIIAPPERKYSVWIGGSILASLTTFQQMWISKQ 360
GGTTM+PGIA+R+QKEITALAPSSMK+K++APPERKYSVWIGGSILASL+TFQQMWISKQ
Sbjct 165023 GGTTMYPGIADRVQKEITALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWISKQ 164844
Query 361 EYDESGPSIVHHKCF 375
EYDESGPSIVH KCF
Sbjct 164843 EYDESGPSIVHRKCF 164799
> scaffold-514
Length=59281
Score = 717 bits (1851), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 332/375 (89%), Positives = 362/375 (97%), Gaps = 0/375 (0%)
Frame = -2
Query 1 MDSEVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGIMVGMGQKDSYVGDEAQS 60
M+ EVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQG+MVGMGQKDSYVGDEAQS
Sbjct 10368 MEDEVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQS 10189
Query 61 KRGILTLRYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPMNPKSNREKMT 120
KRGILTL+YPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVL TEAP+NPK+NREKMT
Sbjct 10188 KRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLCTEAPLNPKANREKMT 10009
Query 121 QIMFETFNVPAFYVSIQAVLSLYSSGRTTGIVLDSGDGVTHVVPIYAGFSLPHAILRIDL 180
QIMFETFNVPAFYV+I AVLSLY+SGRTTGIVLDSGDGV+H VPIY G++LPHAILR+DL
Sbjct 10008 QIMFETFNVPAFYVAI*AVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDL 9829
Query 181 AGRDLTDYLMKILSERGYSFSTTAEREIVRDIKEKLCYVALDFEQEMQTAAQSSSIEKSY 240
AGRDLTDYLMKIL+ERGYSF+TTAEREIVRDIKEKLCYVALDFE EMQTA+ SS++EKSY
Sbjct 9828 AGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFE*EMQTASTSSALEKSY 9649
Query 241 ELPDGQVITIGNERFRAPEALFHPSVLGLESAGIDQTTYNSIMKCDVDVRKELYGNIVMS 300
ELPDGQVITIGNERFR PEALF PS LG+E+AG+ +TTYNSIMKCDVD+RK+LY NIV+S
Sbjct 9648 ELPDGQVITIGNERFRCPEALFQPSFLGMEAAGVHETTYNSIMKCDVDIRKDLYANIVLS 9469
Query 301 GGTTMFPGIAERMQKEITALAPSSMKVKIIAPPERKYSVWIGGSILASLTTFQQMWISKQ 360
GGTTM+PGIA+R+QKEITALAPSSMK+K++APPERKYSVWIGGSILASL+TFQQMWISKQ
Sbjct 9468 GGTTMYPGIADRVQKEITALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWISKQ 9289
Query 361 EYDESGPSIVHHKCF 375
EYDESGPSIVH KCF
Sbjct 9288 EYDESGPSIVHRKCF 9244
В этом случае выравниваний с нулевыми e-value нет, но есть несколько убедительных находок с выравниванием полной последовательности белка и низкими e-value
Query= sp|P61830|H3_YEAST Histone H3 OS=Saccharomyces cerevisiae (strain
ATCC 204508 / S288c) OX=559292 GN=HHT1 PE=1 SV=2
Length=136
Score E
Sequences producing significant alignments: (Bits) Value
scaffold-126 232 9e-72
scaffold-104 232 9e-72
unplaced-984 174 1e-57
scaffold-153 153 1e-48
scaffold-70 58.5 2e-11
unplaced-368 57.0 1e-10
unplaced-13 29.3 0.36
scaffold-168 27.7 1.8
> scaffold-126
Length=92370
Score = 232 bits (591), Expect = 9e-72, Method: Compositional matrix adjust.
Identities = 119/136 (88%), Positives = 127/136 (93%), Gaps = 1/136 (1%)
Frame = -3
Query 1 MARTKQTARKSTGGKAPRKQLASKAARKSAPSTGGVKKPHRYKPGTVALREIRRFQKSTE 60
MARTK TARKSTGGKAPRKQLA+KA RKS P+TGGVKKPHRY+PGTVALREIRR+QKSTE
Sbjct 85096 MARTK*TARKSTGGKAPRKQLATKA-RKSTPATGGVKKPHRYRPGTVALREIRRYQKSTE 84920
Query 61 LLIRKLPFQRLVREIAQDFKTDLRFQSSAIGALQESVEAYLVSLFEDTNLAAIHAKRVTI 120
LLIRKLPFQRLVREIAQDFKTDLRF SSAIGAL E+ EAYLVSLFEDTNL AIHAKRVTI
Sbjct 84919 LLIRKLPFQRLVREIAQDFKTDLRF*SSAIGAL*EAAEAYLVSLFEDTNLCAIHAKRVTI 84740
Query 121 QKKDIKLARRLRGERS 136
KDI+LARR+RGER+
Sbjct 84739 FPKDIQLARRIRGERA 84692
Score = 57.0 bits (136), Expect = 2e-10, Method: Compositional matrix adjust.
Identities = 32/37 (86%), Positives = 34/37 (92%), Gaps = 1/37 (3%)
Frame = +2
Query 1 MARTKQTARKSTGGKAPRKQLASKAARKSAPSTGGVK 37
MARTK TARKSTGGKAPRKQLA+K ARKS P+TGGVK
Sbjct 92261 MARTK*TARKSTGGKAPRKQLATK-ARKSTPATGGVK 92368
> scaffold-104
Length=736462
Score = 232 bits (591), Expect = 9e-72, Method: Compositional matrix adjust.
Identities = 119/136 (88%), Positives = 127/136 (93%), Gaps = 1/136 (1%)
Frame = +3
Query 1 MARTKQTARKSTGGKAPRKQLASKAARKSAPSTGGVKKPHRYKPGTVALREIRRFQKSTE 60
MARTK TARKSTGGKAPRKQLA+KA RKS P+TGGVKKPHRY+PGTVALREIRR+QKSTE
Sbjct 185496 MARTK*TARKSTGGKAPRKQLATKA-RKSTPATGGVKKPHRYRPGTVALREIRRYQKSTE 185672
Query 61 LLIRKLPFQRLVREIAQDFKTDLRFQSSAIGALQESVEAYLVSLFEDTNLAAIHAKRVTI 120
LLIRKLPFQRLVREIAQDFKTDLRF SSAIGAL E+ EAYLVSLFEDTNL AIHAKRVTI
Sbjct 185673 LLIRKLPFQRLVREIAQDFKTDLRF*SSAIGAL*EAAEAYLVSLFEDTNLCAIHAKRVTI 185852
Query 121 QKKDIKLARRLRGERS 136
KDI+LARR+RGER+
Sbjct 185853 FPKDIQLARRIRGERA 185900
Score = 232 bits (591), Expect = 9e-72, Method: Compositional matrix adjust.
Identities = 119/136 (88%), Positives = 127/136 (93%), Gaps = 1/136 (1%)
Frame = -2
Query 1 MARTKQTARKSTGGKAPRKQLASKAARKSAPSTGGVKKPHRYKPGTVALREIRRFQKSTE 60
MARTK TARKSTGGKAPRKQLA+KA RKS P+TGGVKKPHRY+PGTVALREIRR+QKSTE
Sbjct 178362 MARTK*TARKSTGGKAPRKQLATKA-RKSTPATGGVKKPHRYRPGTVALREIRRYQKSTE 178186
Query 61 LLIRKLPFQRLVREIAQDFKTDLRFQSSAIGALQESVEAYLVSLFEDTNLAAIHAKRVTI 120
LLIRKLPFQRLVREIAQDFKTDLRF SSAIGAL E+ EAYLVSLFEDTNL AIHAKRVTI
Sbjct 178185 LLIRKLPFQRLVREIAQDFKTDLRF*SSAIGAL*EAAEAYLVSLFEDTNLCAIHAKRVTI 178006
Query 121 QKKDIKLARRLRGERS 136
KDI+LARR+RGER+
Sbjct 178005 FPKDIQLARRIRGERA 177958
> unplaced-984
Length=389
Score = 174 bits (442), Expect = 1e-57, Method: Compositional matrix adjust.
Identities = 86/98 (88%), Positives = 92/98 (94%), Gaps = 0/98 (0%)
Frame = +2
Query 39 PHRYKPGTVALREIRRFQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAIGALQESVE 98
PHRY+PGTVALREIRR+QKSTELLIRKLPFQRLVREIAQDFKTDLRF SSAIGAL E+ E
Sbjct 2 PHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRF*SSAIGAL*EAAE 181
Query 99 AYLVSLFEDTNLAAIHAKRVTIQKKDIKLARRLRGERS 136
AYLVSLFEDTNL AIHAKRVTI KDI+LARR+RGER+
Sbjct 182 AYLVSLFEDTNLCAIHAKRVTIFPKDIQLARRIRGERA 295
> scaffold-153
Length=574
Score = 153 bits (387), Expect = 1e-48, Method: Compositional matrix adjust.
Identities = 78/89 (88%), Positives = 83/89 (93%), Gaps = 0/89 (0%)
Frame = -2
Query 48 ALREIRRFQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAIGALQESVEAYLVSLFED 107
ALREIRR+QKSTELLIRKLPFQRLVREIAQDFKTDLRF SSAIGAL E+ EAYLVSLFED
Sbjct 573 ALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRF*SSAIGAL*EAAEAYLVSLFED 394
Query 108 TNLAAIHAKRVTIQKKDIKLARRLRGERS 136
TNL AIHAKRVTI KDI+LARR+RGER+
Sbjct 393 TNLCAIHAKRVTIFPKDIQLARRIRGERA 307