Так как у меня мало опыта в программировании, сначала я хотел считать длины вот так:
In [ ]:
import pandas as pd
from tqdm import tqdm
with open("orca_genomic.fna", "r") as f:
file = f.readlines()
name = None
sequences = dict()
for line in tqdm(file):
line = line.rstrip()
if line[0] == '>':
name = line[1:]
sequences[name] = ''
else:
sequences[name] = sequences[name] + line
df = pd.DataFrame.from_dict(sequences, orient='index', columns=['Sequence'])
df["length"] = df["Sequence"].apply(len)
print(df)
Но с ужасом обнаружил, что это займет, как минимум, сутки. Поэтому я погуглил и сделал так:
In [75]:
from Bio import SeqIO
records = list(SeqIO.parse("orca_genomic.fna", "fasta"))
records
#это заняло 6 секунд
Out[75]:
[SeqRecord(seq=Seq('ggccataagcgagaggtgcgtggtgtcacgtgagctacggtagggggacgtgat...tag'), id='NC_064559.1', name='NC_064559.1', description='NC_064559.1 Orcinus orca chromosome 1, mOrcOrc1.1, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('aaaccctaaccctaaccctaaccctaaccctaaccctaaccctaaccctaaccc...CAA'), id='NC_064560.1', name='NC_064560.1', description='NC_064560.1 Orcinus orca chromosome 2, mOrcOrc1.1, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('GTCCTTCATGTCCAGTCATGGCCCCAAGATCCTTCCCACCGACTTCTGAGCCAA...gtt'), id='NC_064561.1', name='NC_064561.1', description='NC_064561.1 Orcinus orca chromosome 3, mOrcOrc1.1, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('ccctcacccgtcccctaatcctaacccccgtacgctgacactcacccgcgcccc...ggt'), id='NC_064562.1', name='NC_064562.1', description='NC_064562.1 Orcinus orca chromosome 4, mOrcOrc1.1, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('gacaagatctacaccttgctcaatgaactggcctcaacacaccctatacaccgc...ggg'), id='NC_064563.1', name='NC_064563.1', description='NC_064563.1 Orcinus orca chromosome 5, mOrcOrc1.1, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('gctaaattagggagttccagggcaagggtctgaattgactgctctttccattct...CTA'), id='NC_064564.1', name='NC_064564.1', description='NC_064564.1 Orcinus orca chromosome 6, mOrcOrc1.1, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('agggaatgggtacgccttagggtacgacaacaggtcaggcatagggtacgtgtc...tag'), id='NC_064565.1', name='NC_064565.1', description='NC_064565.1 Orcinus orca chromosome 7, mOrcOrc1.1, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('aaccctaaccctaaccctaaccctgaaccctaaccctaaccctaacctaaccct...ttc'), id='NC_064566.1', name='NC_064566.1', description='NC_064566.1 Orcinus orca chromosome 8, mOrcOrc1.1, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('cctaaccctaaccctaaccctaaccctaaccctaaccctaaccctaaccctaac...cct'), id='NC_064567.1', name='NC_064567.1', description='NC_064567.1 Orcinus orca chromosome 9, mOrcOrc1.1, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('tcccctaaccctaaccctagccccagccctaaccctaacccttccccgaagcct...agg'), id='NC_064568.1', name='NC_064568.1', description='NC_064568.1 Orcinus orca chromosome 10, mOrcOrc1.1, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('ccctcacctggaccctaaaccgtccccgtcccctaaccctaaccctagcccgaa...aGT'), id='NC_064569.1', name='NC_064569.1', description='NC_064569.1 Orcinus orca chromosome 11, mOrcOrc1.1, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('cctaaccctaaccctaacctaacctaaccctaaaccctaacccgaaccctaacc...gct'), id='NC_064570.1', name='NC_064570.1', description='NC_064570.1 Orcinus orca chromosome 12, mOrcOrc1.1, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('taacccgtacgctgacactcacccgcgccctcacacgtacgctccccctaaagc...tta'), id='NC_064571.1', name='NC_064571.1', description='NC_064571.1 Orcinus orca chromosome 13, mOrcOrc1.1, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('tagggcagggcatcagggcacttcgccgcacgtggagcaagtttgttctcatct...gtt'), id='NC_064572.1', name='NC_064572.1', description='NC_064572.1 Orcinus orca chromosome 14, mOrcOrc1.1, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('tacgctccccctaaagcgttgtcgtaccctcacctggaccctaagccgtccccg...tta'), id='NC_064573.1', name='NC_064573.1', description='NC_064573.1 Orcinus orca chromosome 15, mOrcOrc1.1, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('agaagcctgaccctcacccgtcccctaatcctaacccgtacgctgacactcacc...agg'), id='NC_064574.1', name='NC_064574.1', description='NC_064574.1 Orcinus orca chromosome 16, mOrcOrc1.1, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('gacactcacccgcgccctcacacgtacgctccccctaaagcgttgtcgtaccct...ggg'), id='NC_064575.1', name='NC_064575.1', description='NC_064575.1 Orcinus orca chromosome 17, mOrcOrc1.1, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('ccgtacgctgacactaacccgtgccctcacacgtacactcaccctaaaccgttg...gta'), id='NC_064576.1', name='NC_064576.1', description='NC_064576.1 Orcinus orca chromosome 18, mOrcOrc1.1, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('TGCCACGACCTTCTAAGTGTCAGTAGGCACATGGAGCTAGCGGCTACTGCATTG...ggc'), id='NC_064577.1', name='NC_064577.1', description='NC_064577.1 Orcinus orca chromosome 19, mOrcOrc1.1, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('aaccctaaccctaaccctaaccctaaccctaaccctaaccctaaccctaaccct...ggt'), id='NC_064578.1', name='NC_064578.1', description='NC_064578.1 Orcinus orca chromosome 20, mOrcOrc1.1, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('AGGGCCCtgatcacccaggggaggaggctcccagcaggctccaactgcgaCCAG...tgt'), id='NC_064579.1', name='NC_064579.1', description='NC_064579.1 Orcinus orca chromosome 21, mOrcOrc1.1, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('aaccctaaccctaaccctaaccctaaccctaaccctaacctaaccctaacccta...cac'), id='NC_064580.1', name='NC_064580.1', description='NC_064580.1 Orcinus orca chromosome X, mOrcOrc1.1, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('cctgcagagttataaatgacagctatcgtccaaaaatatactgaagtaaggctg...att'), id='NW_026043728.1', name='NW_026043728.1', description='NW_026043728.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_202, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('ccaaacccttccccgaagcctgaccctcacccgtcccctaatcctaacccgtat...cta'), id='NW_026043729.1', name='NW_026043729.1', description='NW_026043729.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_180, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('tgggttaagtaccctccggggaaggatcgttgcttgggaccccggggctggtgt...agc'), id='NW_026043730.1', name='NW_026043730.1', description='NW_026043730.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_236, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('AAAAACGCAtagcgccctttttggccaagtgcatcattgtcgaccttctgcctc...aga'), id='NW_026043731.1', name='NW_026043731.1', description='NW_026043731.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_200, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('gcactacaatgaagtctcacttcccccgggtcaaaagggccatctgtaaaaagt...ttc'), id='NW_026043732.1', name='NW_026043732.1', description='NW_026043732.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_457, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('tactcgggtacaacattccagacgctttactaacactctcccgacttggagagt...ttc'), id='NW_026043733.1', name='NW_026043733.1', description='NW_026043733.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_342, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('gcgttcctgcacggaactgagcccgggtgcgtgagaccgtgagagggggcaaag...aac'), id='NW_026043734.1', name='NW_026043734.1', description='NW_026043734.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_172, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('ttaaaatcaaaaagacacagccaccccaaagtttgggatggctctgtttacaag...aag'), id='NW_026043735.1', name='NW_026043735.1', description='NW_026043735.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_72, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('aaacagaacatggtcccaaatttagaaaaccaacttatgcttgcttaaggggaa...caa'), id='NW_026043736.1', name='NW_026043736.1', description='NW_026043736.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_264, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('tgtaacttgccaacagccactcgggagaagtgtatggtgtttcctgaaacatct...gga'), id='NW_026043737.1', name='NW_026043737.1', description='NW_026043737.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_117, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('cctgcaattctgcccggctttcaagtcctcttggcagccttacttcagtatatt...ctt'), id='NW_026043738.1', name='NW_026043738.1', description='NW_026043738.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_363, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('tcgttgagcaaggagtagctcttgtctattacatatttggcttaaggaacttta...gtc'), id='NW_026043739.1', name='NW_026043739.1', description='NW_026043739.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_206, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('caggaaaaaacgcatacgccctttttggccaaccaagcaagcttgcaaaggaaa...agg'), id='NW_026043740.1', name='NW_026043740.1', description='NW_026043740.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_42, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('cgccttcagtgtgaaaggcctctacgcctctctgctttcgcagcccaggcgtag...ggg'), id='NW_026043741.1', name='NW_026043741.1', description='NW_026043741.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_104, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('atcacatggttgatcatactctgggttccacatgcatgttttagctgaaggaag...cac'), id='NW_026043742.1', name='NW_026043742.1', description='NW_026043742.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_121, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('taactctcatttcccaccaggaagaggaattaaccaaaggctcagcgtgccatg...cca'), id='NW_026043743.1', name='NW_026043743.1', description='NW_026043743.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_500, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('cccctgtactcacgattgattcactttcagagacatagcaatccataggtttta...ccc'), id='NW_026043744.1', name='NW_026043744.1', description='NW_026043744.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_41, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('acgcggagcaagtttgttctcatctcgcctctgaatgccgagtttaaccagcta...tta'), id='NW_026043745.1', name='NW_026043745.1', description='NW_026043745.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_311, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('taggtgacgggtgagggtcaggcttcggagaagggttagggttagggttcgggc...tag'), id='NW_026043746.1', name='NW_026043746.1', description='NW_026043746.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_188, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('gctgctgtgtagagatcctgtcagagtcagtgggagggagggggcggagctctc...ttt'), id='NW_026043747.1', name='NW_026043747.1', description='NW_026043747.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_181, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('ccctaaaccgtccccgtcccctaacccgaaccctagcccgaaccctaaccctaa...acc'), id='NW_026043748.1', name='NW_026043748.1', description='NW_026043748.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_301, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('gcagcataatgagtggattaagctatgatgattcttacggaaataagtcacaca...tct'), id='NW_026043749.1', name='NW_026043749.1', description='NW_026043749.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_64, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('acacggttgatcatactctgggttccacatgcatgttttagctgaaggaagaat...cgc'), id='NW_026043750.1', name='NW_026043750.1', description='NW_026043750.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_430, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('aaaaagcgcatacgaactttttggccaaccaagcaagcttgcaaaggaaatctg...aaa'), id='NW_026043751.1', name='NW_026043751.1', description='NW_026043751.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_40, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('ttgaaatatgtctcagttctcgtcccctggtactcgggtgcaacattccagatg...gat'), id='NW_026043752.1', name='NW_026043752.1', description='NW_026043752.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_483, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('cgtaccctcacctggaccctaaaccgtccccgtcccctaaccctaaccctagcc...gaa'), id='NW_026043753.1', name='NW_026043753.1', description='NW_026043753.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_393, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('TTTTTTCAGAttgcccctttgaccgcggggaagtgagacttcattgtagtgcag...taa'), id='NW_026043754.1', name='NW_026043754.1', description='NW_026043754.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_155, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('agcgtacgggttaggattaggggacgggtgagggtcaggcttcggggaagggtt...tta'), id='NW_026043755.1', name='NW_026043755.1', description='NW_026043755.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_339, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('ctaactgaaataagtcacacagaaaaagaaacatcataagatatcactaacaca...gta'), id='NW_026043756.1', name='NW_026043756.1', description='NW_026043756.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_290, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('gttttaagatacttactagtcaggtacattcttaggcgtttaatatggggtgtt...gga'), id='NW_026043757.1', name='NW_026043757.1', description='NW_026043757.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_123, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('ttttctgtgtgacttatttcagtgagaatcatcatacctgaatccagtctatgc...cag'), id='NW_026043758.1', name='NW_026043758.1', description='NW_026043758.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_86, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('tattacatatttggcttaaggaaatttatctgtgttcatttcaatctctggttt...agt'), id='NW_026043759.1', name='NW_026043759.1', description='NW_026043759.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_81, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('tgccctaggctctgttgctttgttttttagatgtttcaggaaacaccatacact...cct'), id='NW_026043760.1', name='NW_026043760.1', description='NW_026043760.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_173, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('agatgtcattgctgagtgatattgcattgtacgtaagtaccacaacttctttat...tac'), id='NW_026043761.1', name='NW_026043761.1', description='NW_026043761.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_384, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('taacctcctgtttggcccagtttgcaaattccacggaagatgaacaggaatagg...tag'), id='NW_026043762.1', name='NW_026043762.1', description='NW_026043762.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_177, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('gaagtcatattgcatggtacccattccacgggtctcaactctccaggtttaagg...gaa'), id='NW_026043763.1', name='NW_026043763.1', description='NW_026043763.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_90, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('attagggcctgaagcaatcctgcggtgttgcgggcagctcacaagaaagcgggt...acc'), id='NW_026043764.1', name='NW_026043764.1', description='NW_026043764.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_63, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('tgtgagtaaacgtgagctgagccccaggccagttgctgctgaactattcccacc...aaa'), id='NW_026043765.1', name='NW_026043765.1', description='NW_026043765.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_247, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('accataagtggaagtgccctaggctctgttgctttgttttttagatgtttcagg...ata'), id='NW_026043766.1', name='NW_026043766.1', description='NW_026043766.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_82, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('gaggtagactggaattgcatttaaagcataggaaaagaggcagaacgtccacaa...taa'), id='NW_026043767.1', name='NW_026043767.1', description='NW_026043767.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_503, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('cagctagtctctcattggtcctccctgttcctgttcatcttccgcagaaattgc...tcc'), id='NW_026043768.1', name='NW_026043768.1', description='NW_026043768.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_282, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('aaacaccatacacttctcccgagtggctgttggcaagttacatcccgcccatca...tct'), id='NW_026043769.1', name='NW_026043769.1', description='NW_026043769.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_165, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('gacactttgaagtcatattgcatggtacccattccacgggtctcaactctccag...att'), id='NW_026043770.1', name='NW_026043770.1', description='NW_026043770.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_309, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('gcaagtgccctgatacggttttcctctgtggaactggtgggttctgtgtttttt...ttc'), id='NW_026043771.1', name='NW_026043771.1', description='NW_026043771.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_210, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('TAGTAGTAGTGTTTGCAGCAgtagtagaagtggcagcagcaacagtagtagtag...gcg'), id='NW_026043772.1', name='NW_026043772.1', description='NW_026043772.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_491, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('ggtgagcaaatgaagaccctttgaagtcatattgcatggtacccattccacggg...cgg'), id='NW_026043773.1', name='NW_026043773.1', description='NW_026043773.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_378, whole genome shotgun sequence', dbxrefs=[]), 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description='NW_026044122.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_434, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('agttccttaagccaaatatggaatagacaagagctactccttgctcaacgaaat...tag'), id='NW_026044123.1', name='NW_026044123.1', description='NW_026044123.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_216, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('tgtactcacgcttgattcactttcgagacatagaaatccataggttttaagatt...tta'), id='NW_026044124.1', name='NW_026044124.1', description='NW_026044124.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_154, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('tgacgctggccagaagctcaaacatttagggcaggtcatcagggcacttcgccg...cca'), id='NW_026044125.1', name='NW_026044125.1', description='NW_026044125.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_276, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('AACCTATTTGATTTAGAATCTAAACCCTAGAAGCATACACAGGGcttaaagaaa...GAG'), id='NW_026044126.1', name='NW_026044126.1', description='NW_026044126.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_437, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('Ggcttggctgcttcagtgtgtgagtgtaggcctgtggctcttagaaacgggata...AAC'), id='NW_026044127.1', name='NW_026044127.1', description='NW_026044127.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_390, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('gaagtgagacttcattgtagtgcagatttcctttgcaagcttgcttggttggcc...tcc'), id='NW_026044128.1', name='NW_026044128.1', description='NW_026044128.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_203, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('ctattcctgttcatcttctgcagaaattgcaaactgggccaaacaggaggttaa...tcc'), id='NW_026044129.1', name='NW_026044129.1', description='NW_026044129.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_57, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('ggtaccatgcaatatgacttcaaagggtcttcatttgctcaccaaaaactctcc...aac'), id='NW_026044130.1', name='NW_026044130.1', description='NW_026044130.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_89, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('tttcttgagtgagtcgtactgctataaggcaaaactccctgctggaatctcggt...aat'), id='NW_026044131.1', name='NW_026044131.1', description='NW_026044131.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_115, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('gcaacagagcctagggcacttccacttatggtcctatagcttagggaaattaaa...tag'), id='NW_026044132.1', name='NW_026044132.1', description='NW_026044132.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_268, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('caacgctttagggggagcgtacgtgtgagggcgcgggtgagtgtcagcgtacgg...ggt'), id='NW_026044133.1', name='NW_026044133.1', description='NW_026044133.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_291, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('gaacgacagcagttttcaaggacggcccgctcagtgcatcggacctaacaccat...atg'), id='NW_026044134.1', name='NW_026044134.1', description='NW_026044134.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_376, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('cgctattcctgttcatcttccgcagaaattgcaaactgtgccaaacaggaggtt...cac'), id='NW_026044135.1', name='NW_026044135.1', description='NW_026044135.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_300, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('tcccgacttggagagtcagtgcctttaacgtcctgtttggcccagtttgcaatt...ttt'), id='NW_026044136.1', name='NW_026044136.1', description='NW_026044136.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_219, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('atactctgggttccacatgcatgttttagctgaaggaagaatcccttaaacctg...tca'), id='NW_026044137.1', name='NW_026044137.1', description='NW_026044137.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_272, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('ccagaggcgtaactaggcagggcattccttggttgaggcagttcacttgaccca...gca'), id='NW_026044138.1', name='NW_026044138.1', description='NW_026044138.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_193, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('gctcttgtctattacatatttggcttaaggaactttatctgtgctcatttcaat...tga'), id='NW_026044139.1', name='NW_026044139.1', description='NW_026044139.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_48, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('aaaacgcatacgccctttttggacaaccaagcaagcttgcaaaggaaatctgca...aac'), id='NW_026044140.1', name='NW_026044140.1', description='NW_026044140.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_161, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('gagactagctggaggtgtctggacgggaaaatttaactctcatttcccaccagg...tga'), id='NW_026044141.1', name='NW_026044141.1', description='NW_026044141.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_418, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('caccccaaagtttgggatggctctgtttacaaaaaactcgtttacggtacaagt...aaa'), id='NW_026044142.1', name='NW_026044142.1', description='NW_026044142.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_327, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('ttaaatgcatttccagtctacctcctgaaatcggtttcctgcaattctgcccca...cta'), id='NW_026044143.1', name='NW_026044143.1', description='NW_026044143.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_465, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('cactaatacacggaatgtaagcttggctacacaggaactggattacaaaacaca...aaa'), id='NW_026044144.1', name='NW_026044144.1', description='NW_026044144.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_248, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('ggaagatgaacaggaatagggagaaccaatgagagactagctggaggtgtctgg...aaa'), id='NW_026044145.1', name='NW_026044145.1', description='NW_026044145.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_128, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('aattcagaaaggcaggacaggccatggagaactgggagccttgttatgctgatg...tga'), id='NW_026044146.1', name='NW_026044146.1', description='NW_026044146.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_462, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('aagcctgaccctcacccgtcccctaatcctaacccgtacgctgacactcacccg...gta'), id='NW_026044147.1', name='NW_026044147.1', description='NW_026044147.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_360, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('ctccgaaacagaatcaagcgtgtgtacaggggcataaacgcagcagtgatagga...aag'), id='NW_026044148.1', name='NW_026044148.1', description='NW_026044148.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_145, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('ttgactctccaagttgggagagtgttagtaaagcatctggaatgttgcacccga...tac'), id='NW_026044149.1', name='NW_026044149.1', description='NW_026044149.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_218, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('cccaccaaacccacactttcacgaacagacttctggcacgtctcacatgccttt...ggt'), id='NW_026044150.1', name='NW_026044150.1', description='NW_026044150.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_510, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('ccagttgtaaacaattctgaagttggtcagtggtggggaatcatctcccattca...tta'), id='NW_026044151.1', name='NW_026044151.1', description='NW_026044151.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_446, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('tgtagtgcagatttccgttgcaatcttgcttggttggccaaaaagtgcgtatgc...ttg'), id='NW_026044152.1', name='NW_026044152.1', description='NW_026044152.1 Orcinus orca unplaced genomic scaffold, mOrcOrc1.1 scaffold_267, whole genome shotgun sequence', dbxrefs=[]), SeqRecord(seq=Seq('TTGATGTAGCTTAAATTTTTATAAAGCAAGACACTGAAAATGTCTAGATGGACA...CCG'), id='NC_064558.1', name='NC_064558.1', description='NC_064558.1 Orcinus orca mitochondrion, complete genome', dbxrefs=[])]
In [31]:
lengths = []
for record in records:
lengths.append(len(record.seq))
lengths.sort(reverse=True)
lengths
Out[31]:
[212697510, 201926916, 185155944, 154893898, 149304827, 132499998, 120877333, 120755892, 114219206, 105981142, 105965762, 102994062, 93388644, 91537726, 90513360, 89590773, 87999559, 87542868, 78603316, 61273037, 59094136, 35416747, 9976000, 3172000, 2614540, 2544000, 2384740, 2150776, 2143944, 2059315, 1945816, 1933047, 1887635, 1833689, 1720993, 1692000, 1686000, 1659817, 1597000, 1596250, 1565731, 1500002, 1494000, 1485000, 1453898, 1412000, 1408474, 1408085, 1406000, 1387224, 1386055, 1360270, 1241919, 1207000, 1186330, 1183510, 1127000, 1120375, 1116818, 1116386, 1092000, 1069812, 1062300, 1060343, 1051474, 1033394, 986974, 979455, 971927, 959109, 924831, 917057, 914053, 894160, 879000, 876000, 867000, 834000, 826000, 825000, 783850, 773928, 755000, 753000, 750195, 744051, 735078, 727188, 706972, 704706, 696188, 692708, 687532, 673602, 670000, 668426, 666760, 658798, 654435, 644000, 631000, 615057, 613717, 612889, 610632, 597661, 592000, 582500, 556589, 545135, 539717, 537754, 531373, 529000, 524193, 523566, 515000, 503000, 486248, 482000, 474545, 471869, 471000, 465119, 463728, 428225, 425160, 425011, 421375, 415000, 413225, 413000, 412941, 406249, 401744, 396142, 389000, 387955, 385457, 376723, 370845, 366521, 365330, 359343, 358147, 357088, 355835, 352771, 352744, 350002, 348441, 343126, 340000, 336970, 333496, 331343, 330212, 326842, 324038, 321676, 317065, 315929, 312000, 310381, 305385, 304000, 303914, 303854, 301266, 297000, 296585, 292000, 287000, 286529, 283863, 277718, 274000, 273962, 271327, 268025, 266170, 256131, 256000, 254125, 250918, 245200, 239826, 236166, 235992, 232863, 232647, 232310, 232259, 228000, 227019, 224762, 223000, 221505, 220519, 219000, 217000, 214304, 213944, 210744, 209350, 208564, 208000, 207000, 206809, 206142, 205978, 203502, 199936, 199000, 196678, 196529, 195443, 195225, 191000, 180334, 177241, 175731, 174850, 174000, 172637, 171274, 170000, 170000, 166000, 165716, 165052, 163546, 160333, 159729, 159192, 158000, 156295, 156038, 156000, 156000, 155435, 153000, 152911, 152000, 150985, 149000, 149000, 147749, 146706, 146594, 146000, 145000, 143125, 142000, 138000, 137368, 136000, 134000, 133322, 132414, 131738, 130694, 130000, 128015, 128000, 127213, 127000, 125322, 125000, 125000, 123522, 123000, 123000, 122544, 121649, 121000, 121000, 119000, 118000, 118000, 117000, 116792, 116667, 114548, 114212, 114113, 114054, 113000, 111000, 110000, 109244, 109000, 108000, 107580, 104097, 103918, 103601, 102658, 102000, 102000, 101205, 100000, 99499, 98943, 97000, 97000, 94598, 94568, 94000, 93208, 93000, 92821, 91468, 91000, 90039, 90000, 90000, 89550, 89380, 89000, 89000, 88983, 88000, 85372, 85000, 84000, 83743, 83561, 83428, 82000, 81000, 80788, 80000, 80000, 79598, 78000, 78000, 77674, 76000, 74555, 74240, 72573, 72000, 71000, 71000, 70555, 70000, 70000, 69982, 69000, 69000, 68909, 68000, 68000, 67345, 67005, 66251, 66018, 66000, 63968, 63311, 63073, 63000, 61588, 61000, 60547, 60017, 60000, 59685, 59352, 59181, 59000, 59000, 58473, 58000, 58000, 57200, 57000, 57000, 56926, 56896, 56000, 56000, 56000, 55000, 54895, 54300, 54138, 53698, 53028, 53000, 53000, 53000, 52605, 52000, 52000, 52000, 51623, 51000, 50993, 50701, 50492, 50264, 49860, 47108, 46000, 46000, 45013, 44403, 42000, 41875, 41847, 41000, 39087, 39000, 38035, 37604, 37475, 37345, 36926, 34000, 33276, 33269, 33000, 33000, 32000, 31000, 30477, 30000, 28000, 28000, 28000, 27752, 24615, 23397, 22523, 21702, 21462, 21000, 21000, 21000, 20247, 20000, 19591, 19000, 16392, 15792, 15590]
In [39]:
import matplotlib.pyplot as plt
import numpy as np
In [94]:
x = np.arange(1, len(lengths) + 1)
y = np.array(lengths)
fig, ax = plt.subplots(figsize = (10, 5))
ax.plot(x, y, zorder=1)
plt.scatter(9, 114219206, c=['#f20f0f'])
plt.xlabel('scaffolds')
plt.ylabel('lenght')
plt.title("total")
plt.ylim([-10000000, 250000000])
plt.show()
In [92]:
without_chr = records[22:-2]
lengths_without_chr=[]
for record in without_chr:
lengths_without_chr.append(len(record.seq))
lengths_without_chr.sort(reverse=True)
x = np.arange(1, len(lengths_without_chr) + 1)
y = np.array(lengths_without_chr)
fig, ax = plt.subplots(figsize = (10, 5))
ax.plot(x, y)
plt.xlabel('scaffolds')
plt.ylabel('lenght')
plt.title("unplaced scaffolds")
plt.ylim([-1000000, 11000000])
plt.show()
In [70]:
lengths_without_chr
Out[70]:
[9976000, 3172000, 2614540, 2544000, 2384740, 2150776, 2143944, 2059315, 1945816, 1933047, 1887635, 1833689, 1720993, 1692000, 1686000, 1659817, 1597000, 1596250, 1565731, 1500002, 1494000, 1485000, 1453898, 1412000, 1408474, 1408085, 1406000, 1387224, 1386055, 1360270, 1241919, 1207000, 1186330, 1183510, 1127000, 1120375, 1116818, 1116386, 1092000, 1069812, 1062300, 1060343, 1051474, 1033394, 986974, 979455, 971927, 959109, 924831, 917057, 914053, 894160, 879000, 876000, 867000, 834000, 826000, 825000, 783850, 773928, 755000, 753000, 750195, 744051, 735078, 727188, 706972, 704706, 696188, 692708, 687532, 673602, 670000, 668426, 666760, 658798, 654435, 644000, 631000, 615057, 613717, 612889, 610632, 597661, 592000, 582500, 556589, 545135, 539717, 537754, 531373, 529000, 524193, 523566, 515000, 503000, 486248, 482000, 474545, 471869, 471000, 465119, 463728, 428225, 425160, 425011, 421375, 415000, 413225, 413000, 412941, 406249, 401744, 396142, 389000, 387955, 385457, 376723, 370845, 366521, 365330, 359343, 358147, 357088, 355835, 352771, 352744, 350002, 348441, 343126, 340000, 336970, 333496, 331343, 330212, 326842, 324038, 321676, 317065, 315929, 312000, 310381, 305385, 304000, 303914, 303854, 301266, 297000, 296585, 292000, 287000, 286529, 283863, 277718, 274000, 273962, 271327, 268025, 266170, 256131, 256000, 254125, 250918, 245200, 239826, 236166, 235992, 232863, 232647, 232310, 232259, 228000, 227019, 224762, 223000, 221505, 220519, 219000, 217000, 214304, 213944, 210744, 209350, 208564, 208000, 207000, 206809, 206142, 205978, 203502, 199936, 199000, 196678, 196529, 195443, 195225, 191000, 180334, 177241, 174850, 174000, 172637, 171274, 170000, 170000, 166000, 165716, 165052, 163546, 160333, 159729, 159192, 158000, 156295, 156038, 156000, 156000, 155435, 153000, 152911, 152000, 150985, 149000, 149000, 147749, 146706, 146594, 146000, 145000, 143125, 142000, 138000, 137368, 136000, 134000, 133322, 132414, 131738, 130694, 130000, 128015, 128000, 127213, 127000, 125322, 125000, 125000, 123522, 123000, 123000, 122544, 121649, 121000, 121000, 119000, 118000, 118000, 117000, 116792, 116667, 114548, 114212, 114113, 114054, 113000, 111000, 110000, 109244, 109000, 108000, 107580, 104097, 103918, 103601, 102658, 102000, 102000, 101205, 100000, 99499, 98943, 97000, 97000, 94598, 94568, 94000, 93208, 93000, 92821, 91468, 91000, 90039, 90000, 90000, 89550, 89380, 89000, 89000, 88983, 88000, 85372, 85000, 84000, 83743, 83561, 83428, 82000, 81000, 80788, 80000, 80000, 79598, 78000, 78000, 77674, 76000, 74555, 74240, 72573, 72000, 71000, 71000, 70555, 70000, 70000, 69982, 69000, 69000, 68909, 68000, 68000, 67345, 67005, 66251, 66018, 66000, 63968, 63311, 63073, 63000, 61588, 61000, 60547, 60017, 60000, 59685, 59352, 59181, 59000, 59000, 58473, 58000, 58000, 57200, 57000, 57000, 56926, 56896, 56000, 56000, 56000, 55000, 54895, 54300, 54138, 53698, 53028, 53000, 53000, 53000, 52605, 52000, 52000, 52000, 51623, 51000, 50993, 50701, 50492, 50264, 49860, 47108, 46000, 46000, 45013, 44403, 42000, 41875, 41847, 41000, 39087, 39000, 38035, 37604, 37475, 37345, 36926, 34000, 33276, 33269, 33000, 33000, 32000, 31000, 30477, 30000, 28000, 28000, 28000, 27752, 24615, 23397, 22523, 21702, 21462, 21000, 21000, 21000, 20247, 20000, 19591, 19000, 15792, 15590]
In [90]:
only_chromosomes = records[:22]
lengths_only_chr=[]
for record in only_chromosomes:
lengths_only_chr.append(len(record.seq))
lengths_only_chr.sort(reverse=True)
x = np.arange(1, len(lengths_only_chr) + 1)
y = np.array(lengths_only_chr)
y2 = []
fig, ax = plt.subplots(figsize = (10, 5))
ax.plot(x, y, zorder=1)
plt.scatter(9, 114219206, c=['#f20f0f'])
plt.xlabel('chromosomes')
plt.ylabel('lenght')
plt.title("only chromosomes")
plt.ylim([-10000000, 250000000])
plt.show()
In [73]:
lengths_only_chr
Out[73]:
[212697510, 201926916, 185155944, 154893898, 149304827, 132499998, 120877333, 120755892, 114219206, 105981142, 105965762, 102994062, 93388644, 91537726, 90513360, 89590773, 87999559, 87542868, 78603316, 61273037, 59094136, 35416747]
Я понимаю, что графики выглядят не очень, но как смог