FastQCFastQC Report
Ñá 29 íîÿ 2014
Ath_tae_CTTGTA_L003_R1_004.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameAth_tae_CTTGTA_L003_R1_004.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC10000.018.50523844-45
TGCCGTC9200.018.051648-49
TATGCCG10250.017.35972846-47
TCGGAAG18100.015.7294513
GAGCACA18550.014.6025719
AGAGCAC18750.014.4462678
ATGCCGT9450.013.5571246-47
GTATGCC10350.013.2949844-45
GATCGGA23950.013.0830011
CGTCTGA14050.013.01905516-17
CGGAAGA22800.012.903214
CACACGT13650.012.88249912-13
GGCCCGG3701.2923332E-512.8347326
GCACACG10400.012.79521410-11
GCCGTCT9700.012.71853448-49
CACGTCT14900.012.280214-15
ATCGGAA25500.011.9162992
AGCACAC16200.011.73458710-11
CTCGTAT15550.011.59663842-43
AGTCACC16300.011.51198428-29