FastQCFastQC Report
Ρα 6 δεκ 2014
trim.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenametrim.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3858095
Sequences flagged as poor quality0
Sequence length50-101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCGG3250.024.5048966
TGGCCCG4100.019.421595
CCCGGGG3851.6370905E-1118.265338
CCGGGGG4253.6379788E-1217.6493249
GCCCGGG4701.8189894E-1115.948537
ATGGCCC7006.730261E-1112.0440264
GGGCCAT6303.0110277E-710.4147621
CGCGGCC3204.123869E-69.52159536-37
GTATGGC8602.5105692E-89.2642942
CGGCCGC3451.0758298E-58.82836138-39
CGGGGGA5653.7107384E-108.71262610-11
GCGGCCG3702.5830783E-58.23489236-37
TCGCGGC3551.4306566E-47.92262734-35
TATGGCC10001.9692976E-57.0256823
CCCCGAA6850.00707155146.8380015
GCCATAC8308.6826645E-46.7717423
ATCGCGG4502.9177667E-46.770911734-35
TATCGCG4553.3388482E-46.696593332-33
GGCCATA9606.410416E-46.3464952
CTAGTAC10854.2269114E-46.04916339