MAST version 4.1.1 (Release date: Tue Apr 21 15:00:06 PDT 2009)

For further information on how to interpret these results or to get a copy of the MAST software please access http://meme.nbcr.net.

If you use this program in your research, please cite:

Timothy L. Bailey and Michael Gribskov, "Combining evidence using p-values: application to sequence homology searches", Bioinformatics, 14(48-54), 1998.

	DATABASE ./seed.fasta (peptide)
	Last updated on Wed May 12 16:53:12 2010
	Database contains 75 sequences, 4650 residues

	MOTIFS ./memeout.txt (peptide)
	MOTIF WIDTH BEST POSSIBLE MATCH
	----- ----- -------------------
	  1    43   TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
	  2    50   RIHPGQKIPMDGTVTWGYSYVNESMITGEPVPVQKKVDSEVIGGTINMHG
	  3    50   CPCAMGLATPTSIMVGTGRAAENGILFRKGEYLQSLHDAQYIAFDKTGTL

	PAIRWISE MOTIF CORRELATIONS:
	MOTIF     1     2
	----- ----- -----
	   2   0.12
	   3   0.12  0.12
	No overly similar pairs (correlation > 0.60) found.

	Random model letter frequencies (from non-redundant database):
	A 0.073 C 0.018 D 0.052 E 0.062 F 0.040 G 0.069 H 0.022 I 0.056 K 0.058 
	L 0.092 M 0.023 N 0.046 P 0.051 Q 0.041 R 0.052 S 0.074 T 0.059 V 0.064 
	W 0.013 Y 0.033 

• Each of the following 68 sequences has E-value less than 10.
• The E-value of a sequence is the expected number of sequences in a random database of the same size that would match the motifs as well as the sequence does and is equal to the combined p-value of the sequence times the number of sequences in the database.
• The combined p-value of a sequence measures the strength of the match of the sequence to all the motifs and is calculated by
  • finding the score of the single best match of each motif to the sequence (best matches may overlap),
  • calculating the sequence p-value of each score,
  • forming the product of the p-values,
  • taking the p-value of the product.
• The sequence p-value of a score is defined as the probability of a random sequence of the same length containing some match with as good or better a score.
• The score for the match of a position in a sequence to a motif is computed by by summing the appropriate entry from each column of the position-dependent scoring matrix that represents the motif.
• Sequences shorter than one or more of the motifs are skipped.
• The table is sorted by increasing E-value.

• The ordering and spacing of all non-overlapping motif occurrences are shown for each high-scoring sequence listed in Section I.
• A motif occurrence is defined as a position in the sequence whose match to the motif has POSITION p-value less than 0.0001.
• The POSITION p-value of a match is the probability of a single random subsequence of the length of the motif scoring at least as well as the observed match.
• For each sequence, all motif occurrences are shown unless there are overlaps. In that case, a motif occurrence is shown only if its p-value is less than the product of the p-values of the other (lower-numbered) motif occurrences that it overlaps.
• The table also shows the E-value of each sequence.
• Spacers and motif occurences are indicated by
• Spacers and motif occurences are indicated by
• Spacers and motif occurences are indicated by

  • n

    occurrence of motif `n' with p-value less than 0.0001.

  • 1

    Sequences longer than 1000 are not shown to scale and are indicated by thicker lines.

• The positions and p-values of the non-overlapping motif occurrences are shown above the actual sequence for each of the high-scoring sequences from Section I.
• A motif occurrence is defined as a position in the sequence whose match to the motif has POSITION p-value less than 0.0001 as defined in Section II.
• For each sequence, the first line specifies the name of the sequence.
• The second (and possibly more) lines give a description of the sequence.
• Following the description line(s) is a line giving the length, combined p-value, and E-value of the sequence as defined in Section I.
• The next line reproduces the motif diagram from Section II.
• The entire sequence is printed on the following lines.
• Motif occurrences are indicated directly above their positions in the sequence on lines showing
  • the motif number of the occurrence,
  • the position p-value of the occurrence,
  • the best possible match to the motif, and
  • columns whose match to the motif has a positive score (indicated by a plus sign).

     [1]
     5.9e-30
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
     ++++++ +++++++++++++++  +++++++++++++++++++
1    TYRVDGLSCTNCAGKFEKNVKNISGVTDAKVNFGAGKITVYGETSISQIEKAGAFENLKISPEK

     [1]
     1.4e-17
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
     ++ ++ + +  +++++ +++ ++++++++ + ++ + + ++++
1    TLEVPTMNCPVCPITVTKSLEKVNGVTDVEVKFEERLAKVTFDDEMTNTKALIDATTNAGYP

     [1]
     6.9e-16
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
      + +++  +++++++ + +++ + ++  +  +++++ ++++++
1    ELIIEGAGCASCVGKIESALKSVSGVENAEMNFAQRTVSVTGTASPSALVKAVERAGYS

     [1]
     8.8e-14
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
      +++ ++++ ++ +++   +++  ++++++    +++++++
1    VYQVSGMSCGHCEGSVSSEIAEIAGVSSVKAVASTGEVTVVSAAPLDDEAVRAAVDEAGFE

     [1]
     5.4e-14
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
     ++ + ++ + +++ + +++++ +++++++++  + ++   +
1    TFKVGGMDCGSCAAKIETALSRLPGVADVKVSVARERLNLSLAENKTPVEKIEDTLRKLGFK

     [1]
     1.3e-12
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
     ++ + ++ +  ++++++  ++ + ++   + ++ ++   ++++
1    QFTIKGMDCPDCARTVERGVARLAGVKTCHLNFTTEQLHVTGDVDPETVIARVRELGYE

     [1]
     9.5e-13
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
     ++++ ++ +  +++++   ++ + ++  +++ +  + +++++
1    TYRVSGMKCDGCAKTVSDKLSSVIGVDEVNVDLTKNQVVVSGKTFKWLLKRSLKDTKYS

     [1]
     2.0e-13
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
     ++ + ++ +  ++++++  +++ ++   ++++++++ +    +
1    QLNINGMKCGGCVNTVENILKNSDGIENVSVNLLTESAYIEVNKTFQNIDQVLENLNQSGFP

     [1]
     4.8e-13
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
     ++ ++++ +++++ +  +   +++++ +++  ++ ++   + +
1    DLPIEGMHCASCVLSLNKTFEKVEGVESVDADLASNKLHLTVNPKKLPFDEIETLVKNLGFE

     [1]
     3.3e-13
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
      ++++ + + +++ ++ +++++++ + + +   +++  +++ +
1    RLSVPEMDCPSCAQKVITSVKKLDAVQDIDSQVATGVLTVSYDADQVTLNEIANQIQNAGYS

     [1]
     3.4e-12
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
     +++++++++ ++  +++ +   +++   +++ +   +++   +
1    TVSVEGMTCGACTSAVEGAFNGVEGAGEVSVSLMMSRAVIHHDPTLLPPGKVAEIIEDCGFD

     [1]
     2.9e-12
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
     ++ ++++ + ++    + +++ +  ++++ + ++ + +++  +
1    TFIIDGMHCKSCMSNIENALSTLQYVSSVVVSLENRSAVVKYNASLVTPETLKKAIEAISPGQYR

     [1]
     3.6e-11
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
      + ++ + + +++++  + +++ +  +  + +++   ++++ +
1    VFKVEDMACNACVNTITKTLSNNENISNISCNLESKDVSVTYDEEKVDVEEIISALDNIGFS

     [1]
     4.5e-11
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
      +  ++  +++++ + ++ +++ +++ + ++++++   +
1    KLMLEGLDCANCAMKIEKGVGNIEGVNSCSVNFATKTMVLETAQNKESEVVTEAKQLVTKLEPHIKVQ

     [1]
     5.4e-11
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
     ++ + + ++  ++  +  +++ ++++ ++++ + +  + +  +
1    TLNVFGTTCRGCAQHVQENLMTLEGVHSVSVDLDAQLAEVDVDATDAATEFRIEKKMVSMGYT

     [1]
     5.3e-10
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
     ++ ++ +++  ++ ++++ +  ++  + +    +++ +++
1    QFHIDDMTCGGCASTVKKTILTLDANATVRTDPATRLVDVETSLSAEQIAAALQKAGFP

     [1]
     2.5e-11
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
     ++ ++++ + ++  +++++  +++ ++ + +  +   ++ +
1    TVYIEGMMCGHCEARVKKAFEALPQVTEAEVSHEKKQAVLTLSGELSDKEIKNTVKEAGYK

     [1]
     3.2e-10
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
     + ++ ++++++++   + ++ + ++    ++++++       +
1    DIRISGMTCSSCVHLIESSLIKRPGILQTSVALATSSGRFKYDTEITGPRDIIEAIKGLGFG

     [1]
     2.0e-10
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
        + ++++++++  ++ ++ + +++ ++ +     ++ +  +
1    EITVNGMTCTSCATHVKDALEKIPGVNAAVVSYPESRAQVMADTAVSHNQLLAAIAALGYQ

     [1]
     1.5e-11
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
      + ++++ +  ++ ++  ++ + + + ++ +++ ++ + + +
1    LFAIEGMRCGGCSAAVRNALDARDDVEAAAVNLVTETAAVRFKTTSMIVGSLDASIESAVAE

     [1]
     1.6e-10
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
      + + ++++  +   ++  ++   +++ +++ ++ +++  + +
1    AYTIKGMTCNGCRTGVEQKLAGIAGVSQATVSLENEEAILTMDDEVTLDHLKSALPEKYR

     [1]
     4.5e-11
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
     +++ +  +++++++ +       ++++++ + ++   + ++ +
1    TVQLENLSCQNCVKHVTQHFLSMEGVSDVVVDLEKQTAQVTTDKLYGASDYEAALAKTIYK

     [1]
     6.7e-10
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
      +    + ++++  ++ + +++ ++  +  + ++  ++++ ++
1    VVMSANMGCSHCRQRVTKVVSKMNGLLDYMVDFGKKEVTVRGTMVHTKKKRKQHKKKHEENKK

     [1]
     3.7e-10
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
     + +  ++++ +++++    + +++ ++ + + ++   +   ++
1    TASLGGMTCGSCVGTITRGIQELPFVTNVVVDLLGSGAKFEFEGRERLDGIKQVVEGLGYE

     [1]
     4.5e-08
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
      + +    +++++      ++++ ++ + ++  ++ ++ +  +
1    LFFVSNVHCSSCVAYITEVLSEVSGVHDIDVSILTHEVRVLHNSETKPSVLAKALIGAAFE

     [1]
     7.6e-09
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
      +  +   ++++   ++  +++++ + ++ +++ + ++  +
1    YLALPQIRCAACIAGVEGVLAKLPSVKAARVNLSAKRVRLVLAPGYDVSTALQALNDAGFD

     [1]
     7.9e-10
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
        +  +     +  +++ ++++ ++ +++++ +++ +++  +
1    VIAVRDMLSVLSVLEVEKRIGEVRGVKSVTVNYAAGNATVRYDETQLEVGDIKSAVRQHSYE

     [1]
     3.2e-08
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
     ++ + +  +  ++++    + +++++ ++++ ++        +
1    TLTIKGIHCGCCVKSLTQVLTELDGVQSADVQLEGKANITFDENRVNVAQLIEVIEDAGFD

     [1]
     4.0e-08
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
      ++++++ + +++ ++  ++++ + +       + + +  +
1    SVRVDGMFCHHCPERVLDSLKAFPDVECERTLSLNNNVLKVTYTPDPPSLTIRAIIQTIESAHD

     [1]
     4.1e-09
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
      + + +++++ ++ + +   ++ +  + +++ +   ++  + +
1    EVEVYGMTCAFCVDSLERKFANMESISQVDVSLKHKKVRLVTDETMPSIETIKQAVLDAGFT

     [1]
     3.5e-09
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
      +      +++++ + +  +++++++ +++  + + + +   +
1    RYNLSDVDCANCAAKMEEQISKLPGVNSASISFMAQKLTLDADEDKLDEILKQAEKIIKSIDEE

     [1]
     4.1e-07
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
        + +  +++++++ + +++ +    ++++ + +  +
1    LIAIQGAGCQSCVKKIKHALAPLTDADKVTVDLQAQTIAIDAAVDIPAALEAIRQLGYP

     [1]
     1.0e-08
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
     ++ ++ +++ ++ +   +++++ ++ ++ +    +  +
1    TFNVPDMTCGHCEKVITKAVKETDGNAKIHFDMKAKTVAIDSSFPSRLLQQAIEATGYE

     [1]
     1.8e-06
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
      +     ++  +  + +++ + + ++ +++   +   +  + +
1    VLFAVRGSCDMCKERIETAAKGVSGVRSAHWDREKQMIHLQLDPSETLADAVAKAIAAAGHD

     [1]
     4.7e-07
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
         +  ++ ++  + + +     ++ ++++ + + ++   ++
1    IMKLDELSCPSCLAKIEGAMTGTKGVISAKVLFNASKVKAEFDENIVKSDELVTKVEKLGYP

     [1]
     3.8e-06
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
      +   +  +++++ + + ++ +++    ++ ++         +
1    KLLLKGLHCANCATKIERAVQKLNIIEEANYNFNNSTLIINLEETHKDSIIKTIQEIVDRIEPG

     [1]
     2.0e-08
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
      + +  + ++++ ++++ +++   +    +  +  + ++ + +
1    VFKVAQMECANCERKVKNNIKFEKGLKNFTTDLKERTVTITYDAEKTNVEKLKEGFRKFKYE

     [1]
     2.2e-07
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
     ++ ++ + +++++ + + + ++++   ++  +        +
1    TLYVPTMNCASCVAKIERAFSALEQKKSAINARVNLADKQVIVEGEICAQLAIETIASVGYA

     [1]
     7.8e-08
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
     ++ ++  ++ ++    +  +  + +  ++ ++   +++ ++
1    TLTVENITCGACGWLIERELLRLKGIQKAVVNVTSRRVQVSWSPAQLKLSDILRVLANIGYK

     [1]
     7.7e-08
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
     ++++ ++++++++++ ++ +   + + +            +
1    TLDIIGMTCAACSNRIEKKLNRMNHVQAKVNLTTEKATIDYESDDYHLEDFVEQIQSLGYD

     [1]
     7.3e-08
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
      + ++++ ++ +    +    +++++ ++    ++  + +  +
1    FFAVDGMHCTTCEAFLEGRAEALDGVYSVEASYATDTVRVNYDPEAVDESALPEQLSGYGYE

     [1]
     7.0e-06
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
      + ++++   ++++ +  ++  +++    +   +     +  +
1    RLTVPGMGSDHCAGLVSQSVRRLPGIRELKTDIANTLLMVELEPSPGADAQAVKAAVEKAGYD

     [1]
     1.6e-06
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
        +    ++ ++++    ++++ + ++ ++  +  +++
1    SIIVQNLKCSGCANTITNKIKELKGITDIHVEVEESKVSFNYLDELDAVAVKEKLKNLGYP

     [1]
     8.3e-06
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
      +++    + +++ + + +++ ++ + + + +        +
1    RFQVFDVECGNCVYKIKRALATLPDVVDINITPDFDNSKAVVILRYKGNLDKREIEEILKEISEESPYH

     [1]
     4.3e-07
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
      + + +   ++     ++ ++  +++ ++  +  +  ++++ +
1    VLEVSGVHWASSKSVAEAVLSRQPGVLSVEANPVAQTATVTYDPRRTDLVRLRDWVRECGYH

       [1]
       7.5e-05
       TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
       ++  +   ++  + ++ + +++ +        ++ ++   +
1    KFDVTMTCGGCSGAVDRVLKRLKENESNIVSYTVLLDEKSANVTVDDESPALYEKILRTIKKTGKG

     [1]
     1.2e-06
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
     ++ +    +  ++++ +  +  +++ + ++   ++   +  +
1    TYTIHNLHCRGCAIRIEEQIQSLPGISFASIDELTHHLVIEGSAPDLIELNKIAGEILPGVYF

     [1]
     1.3e-05
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
                + ++ +++++ + +  ++ +   + ++++ +
1    VEMKVPLYSYGCEKKIKKALSHLRGIHSVQVDYQQQKVTVWGICNRDDVLAAVRKKRRA

     [1]
     3.8e-06
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
      + ++ + +++    +  +++  +++ +    +  +   +  +
1    EYLVPKMDCSAEEQMVRMALSSIDGVQKLIFDLPNRSLKVLHNQKDENITTKLEALGFG

     [1]
     3.2e-05
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
      +           ++    ++  +++++ +    +++ +++++
1    AVLQLSIHEERIRKKAFVTVSRCPGVTSITMDDKTGKMTVVGEVDVPVIVMKLRKLCNTELVSVE

     [1]
     3.2e-05
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
      ++++ + ++         ++ +++  + +  + ++  +
1    RFRIPAMDCAVEEADIRRVLAGVPGIRSLNFQLVARTLTIDAQAEALQAAVSAIGQAGYK

     [1]
     8.0e-05
     TFQVEGMTCQHCVRRVEKNIMNVPGVSKAHVNFETERADVQFD
      + ++  ++ ++  +  +++ +           + + +  + +
1    LLKVPDISCNHCKMRIQKALEEIGEKDFEVKVAEKEVIIDTENIEKVVKKLEEIDYP

                 [3]
                 7.2e-05
                 CPCAMGLATPTSIMVGTGRAAENGILFRKGEYLQSLHDAQYIAFDKTGTL
                      ++    ++  +     ++   + + +   ++ +  +    + +
1    TVRVPLHCAECAARVKEVLLEHKSIYAAKIDLGKNLCVVEGVIEEKKLFEYIYHRTRKYGFIEKVEKKEIIVEEK

CPU: kodomo-count
Time 0.072004 secs.

mast ./memeout.txt -d ./seed.fasta -ev 10.000000 -mt 0.000100