FastQCFastQC Report
Вс 25 фев 2018
F_cleaned.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameF_cleaned.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3444237
Sequences flagged as poor quality0
Sequence length32-100
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135970.3947753885693696No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62230.1806786234512898No Hit
GTAACATTTTCATCCATTCTGTGTAGCATTTTCTGCCACCTTTCACTAGC57130.16587128005418908No Hit
GTTTGAAGGTGTTCGAAAACAATACTCTCTCGAGAAAAGTACATATATAA56650.16447764773446194No Hit
GAAAAATGTAGCGATCAAGTCATGTCTGAGATTCTTCAAAACATACCAAC42810.12429458251566312No Hit
CAAGTTTGAAGGTGTTCGAAAACAATACTCTCTCGAGAAAAGTACATATA41510.12052016164973549No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37000.10742582464563269No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35470.10298362162650247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTCT9950.079.780894
GCCCCCG200.003711576361.5237351
CCCCGGG402.5037298E-761.5237351
GGGCGGG402.5037298E-761.5237351
CCGCAGT7350.061.00145794
CGGGGGA2300.058.848791
GGCCCAC501.7776983E-857.4221531
CGCTGGG601.2132659E-954.6877671
CGGGGGG900.054.6877631
TCGGAAG7300.051.35054494
CCCCAGG401.8004284E-551.269781
AGGCCGG1900.049.6507343
CGCGCAA250.0089736949.2189871
ACGTCTC7550.048.60479492-93
GCCGGTA10450.047.8845021
GCCCAGG704.7548383E-946.8752251
CCCCCCT355.753199E-446.8752251
GGGGGCG355.753199E-446.8752251
CGACGGG453.612117E-545.5731351
TACGTCT8550.043.71484492-93
Produced by FastQC (version 0.11.2)