FastQCFastQC Report
Вс 25 фев 2018
F_without_adapters.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameF_without_adapters.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3869420
Sequences flagged as poor quality0
Sequence length1-100
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT458751.18557820035044No Hit
ATACGACTCACTATAGGGAGACGCGTGTTTTTTTTTTTTTTTTTTTTTTT75820.19594667934729237No Hit
CGGTAATACGACTCACTATAGGGAGACGCGTGTTTTTTTTTTTTTTTTTT64600.1669500855425361No Hit
CGACTCACTATAGGGAGACGCGTGTTTTTTTTTTTTTTTTTTTTTTTTTT61440.15878348693085784No Hit
GTTTGAAGGTGTTCGAAAACAATACTCTCTCGAGAAAAGTACATATATAA60070.15524290462136445No Hit
GTAACATTTTCATCCATTCTGTGTAGCATTTTCTGCCACCTTTCACTAGC59530.1538473466307612No Hit
GAAAAATGTAGCGATCAAGTCATGTCTGAGATTCTTCAAAACATACCAAC50050.1293475507957265No Hit
GCCGGTAATACGACTCACTATAGGGAGACGCGTGTTTTTTTTTTTTTTTT44710.11554703288864998No Hit
CAAGTTTGAAGGTGTTCGAAAACAATACTCTCTCGAGAAAAGTACATATA44140.11407394389856879No Hit
GTCGGTTTCTATTTTCGTAGTTGTAAAGGCTTTGTCTGTCGTTGTTATTA41630.10758718360891298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGTA10650.054.986541
CGTCTCT11100.052.0344894
CGCTTCC5700.051.7721373
CCGCTTC5700.051.7647672
CCGGTAA12900.045.030832
AGGCCGG3000.043.035593
CGGTAAT23850.041.567351
CGGGCGT1157.6397555E-1140.0963131
GGCCGGC758.4908515E-636.8886071
GAGGCCG3500.036.8823932
CGGGGAT2400.036.504351
GTCGGTT25050.035.3423771
GGAGGCC3800.035.189791
GCCCTGC2150.034.3149831
CCCGTGT1900.033.976351
CCGCAGT7250.033.858394
CGGGGGA5500.033.5350951
CATTGGG9000.033.3233687
CGTGCAT2100.032.9362561
TGTAGCG14300.032.588437
Produced by FastQC (version 0.11.2)