UniProt

Tasks

1. This table represents the characteristics of the protein of Lentibacillus amyloliquefaciens

   UniProt ID	UniProt AC	Refseq ID	PDB ID	Length	Molecular weight	Name recommended by UniProt
   A0A0U3W9X5_9BACI	A0A0U3W9X5	WP_068446824.1	---	322	34162 Da	Fructose-1,6-bisphosphatase

   Comments.There is single chain in the protein. The structure is unknown (no PDB ID).
   * It is very strange, because I did pr 1 - 2 (Jmol), and I used the structure of protein 1NI9, which is given in the table.

2. This table represents the results of searching clusters Uniref 50, Uniref90, Uniref100 include my protein.

   The cluster ID	The number of proteins in the cluster	Comments
   UniRef50_A0A0U3W9X5	2016	Organisms: genus Bacillus and many other
   UniRef90_A0A0U3W9X5	1	Organism: only Lentibacillus amyloliquefaciens
   UniRef100_A0A0U3W9X5	1	Organism: only Lentibacillus amyloliquefaciens

3. This table represents the results of different searchings in the UniProt.

   Query text	Number of proteins	Number of proteins, block Reviewed
   name:"fructose 1 6 bisphosphatase"	16350	530
   name:"fructose 1 6 bisphosphatase" taxonomy:"Lentibacillus amyloliquefaciens [1472767]"	2	0
   name:"fructose 1 6 bisphosphatase" taxonomy:"Bacillaceae [186817]"	836	4
   name:"fructose 1 6 bisphosphatase" taxonomy:"Firmicutes [1239]"	3119	59
   name:cytochrome taxonomy:"Phaeophyceae [2870]"	2770	3
   name:cytochrome taxonomy:"Ciliophora [5878]"	1265	6
   name:trypsin	13241	310
   name:trypsin name:inhibitor	2962	209

4. UniProt and RefSeq compare.
   • Both of UniProt and RefSeq entries have some information about history of protein's study and links to articles.
   • The entry in UniProt contains many references to other databases (section DR), unlike RefSeq.
   • The entry in UniProt contains organism's taxonomy (section OC), unlike RefSeq.
   • The entry in RefSeq contains information about regions of linking sites, but there isn't specific ligands which are listed in the UniProt entry.
   • Both of UniProt and RefSeq entries have protein's amino acid sequence.

5. History of this protein's entry.

   This entry appeared on 16 Mar 2016. It was updated five times in 2016 and the last update was on 15 Mar 2017.
   In comparison with the first version, now there are magnified sections DR, KW, FT in the entry. It means that we learned more about it's structure, in particular, about it's link to magnesium ion. And now there is much more articles about this protein and more different entries in other databases.

6. Unusual elements in UniProt. (Help -> Alternative products / Help -> Non-standard -> Non-standard residue / etc.)

   Occurrence	Example
   Alternative products	P04150 (RP ALTERNATIVE SPLICING (ISOFORMS GR-P; GR-A ALPHA AND GR-A BETA; FUNCTION (ISOFORM BETA), AND TISSUE SPECIFICITY (ISOFORM BETA); RP FUNCTION (ISOFORM BETA), SUBCELLULAR LOCATION (ISOFORMS ALPHA AND | RP BETA), AND TISSUE SPECIFICITY (ISOFORM BETA)); P42166, P42167
   Glycosylation	monosaccharides: P54939 (RP PROTEIN SEQUENCE OF 1469-1487 AND 1882-1898, AND GLYCOSYLATION AT | RP THR-1486 AND THR-1889), P13671 polysaccharides: Q15293 (RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-53), P46976, Q9S8M0
   Non-standard residue	P24183 (selenocysteine): FT NON_STD 196 196 Selenocysteine Q8TTA5 (pyrrolysine): FT NON_STD 356 356 Pyrrolysine