FastQCFastQC Report
Вс 28 дек 2014
Trimmomatic_cleaned.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTrimmomatic_cleaned.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3841341
Sequences flagged as poor quality0
Sequence length50-101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCGG2853.012937E-413.1351666
CCCGGGG3004.632175E-412.4826348
GCGGCCG2451.8091505E-611.46274736-37
CGCGGCC2208.8046174E-510.63777636-37
GGCCGCG1800.002135594410.3999638-39
TGGCCCG3800.00334466069.8521445
CCGGGGG4450.00115014029.4671669
CGGCCGC2553.9036918E-49.17643538-39
GGGCCAT5701.1803175E-49.0368981
TCGCGGC3102.952085E-48.30411234-35
GTATGGC8006.9203226E-57.609282
CTAGTAC9901.3165642E-46.61957849
TATCGCG4700.00355720435.975106232-33
GGCCATA10550.00201244825.770072