TBLASTN 2.8.1+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: X5.fasta 1,868 sequences; 23,962,143 total letters Query= KPYK2_YEAST P52489 Pyruvate kinase 2 (PK 2) (2.7.1.40) Length=506 Score E Sequences producing significant alignments: (Bits) Value scaffold-361 520 7e-166 scaffold-115 519 1e-165 scaffold-199 31.2 1.5 scaffold-212 30.8 1.8 scaffold-100 29.6 4.0 scaffold-287 29.3 5.0 scaffold-17 29.3 6.4 > scaffold-361 Length=455836 Score = 520 bits (1338), Expect = 7e-166, Method: Compositional matrix adjust. Identities = 267/492 (54%), Positives = 349/492 (71%), Gaps = 22/492 (4%) Frame = +3 Query 29 IGPKTNSCEAITALRKAGLNIIRLNFSHGSYEFHQSVIENAVKSEQQFPGRPLAIALDTK 88 +GPKTNS + + LR+AG+NI+R+NFSHGSYE+H SVI+NA S Q GRP+AIALDTK Sbjct 218580 VGPKTNSVDMMCKLRQAGMNIVRMNFSHGSYEYHGSVIKNARDSFQ*MSGRPVAIALDTK 218759 Query 89 GPEIRTGRTLNDQDLYIPVDHQMIFTTDASFANTSNDKIMYIDYANLTKVIVPGRFIYVD 148 GPEIRTGR D +L IP H+M+ TTD + AN + +YIDY L G++I+VD Sbjct 218760 GPEIRTGRIKGD*ELSIPHGHEMVLTTDPARANDGDMDCIYIDYEKLPHSTDVGKYIFVD 218939 Query 149 DGILSFKVLQIIDESNLRVQAVNSGYIASHKGVNLPNTDVDLPPLSAKDMKDLQFGVRNG 208 DG L F+V+ I ++RV A NS I+++KGVNLP T V+LP LS +D +DLQFG+ N Sbjct 218940 DGQLRFQVVGI-QGKDVRV*AQNSWKISNNKGVNLPMTTVELPALSERDKRDLQFGIEND 219116 Query 209 IHIVFASFIRTSEDVLSIRKAL----GSEGQDIKIISKIENQQGLDNFDEILEVTDGVMI 264 + ++FASFIR ED+L IR+ L G+DIKII+KIE+ +GLDNFDEIL+VTDG+M+ Sbjct 219117 VDMIFASFIRKKEDILEIRQILDNFMNGRGKDIKIIAKIESHEGLDNFDEILQVTDGIMV 219296 Query 265 ARGDLGIEILAPEVLAIQKKLIAKCNLAGKPVICATQMLDSMTHNPRPTRAEVSDVGNAV 324 ARGDLGIEI A +V QK +IAKCN GKP+ICATQML++MT+NPRPTRAEVSDV NAV Sbjct 219297 ARGDLGIEIAAEKVFIAQKMMIAKCNAVGKPIICATQMLETMTYNPRPTRAEVSDVANAV 219476 Query 325 LDGADCVMLSGETAKGDYPVNAVNIMAATALIAESTIAHLALYDDLRDATPKPtsttetv 384 LDGADCVMLSGETAKG YP+ V++M AES + + L+++LRD TPKPT+TTE + Sbjct 219477 LDGADCVMLSGETAKGQYPLETVDMMNRLCKEAESVLFYPQLFNELRDVTPKPTTTTEAI 219656 Query 385 aaaataaILEQDGKAIVVLSTTGNTARLLSKYRPSCPIILVTRHARTARIAHLYRGVFPF 444 A++A A E AI VL+TTG +ARL+SKYRP P+I VTR+A+ AR HLYRG +PF Sbjct 219657 ASSAVNAAEE*GAAAICVLTTTGESARLISKYRPHIPVITVTRNAKVARQCHLYRGCYPF 219836 Query 445 LYEPKRL-----------------DDWGEDVHRRLKFGVEMARSFGMVDNGDTVVSIQGF 487 +YE R D W EDV R + ++ + GM+ GD +V++QG+ Sbjct 219837 VYEKARKQGSESAPASPNS*SMVHDQWQEDVDERFFWAMKEGKKLGMLKAGDVIVAVQGW 220016 Query 488 KGGVGHSNTLRI 499 +GG G ++ +R+ Sbjct 220017 RGGAGSTSVMRM 220052 > scaffold-115 Length=160031 Score = 519 bits (1337), Expect = 1e-165, Method: Compositional matrix adjust. Identities = 268/492 (54%), Positives = 349/492 (71%), Gaps = 22/492 (4%) Frame = -1 Query 29 IGPKTNSCEAITALRKAGLNIIRLNFSHGSYEFHQSVIENAVKSEQQFPGRPLAIALDTK 88 +GPKTNS + + LR+AG+NI+R+NFSHGSYE+H SVI+NA S Q GRP+AIALDTK Sbjct 32951 VGPKTNSVDMMCKLRQAGMNIVRMNFSHGSYEYHGSVIKNARDSFQ*MSGRPVAIALDTK 32772 Query 89 GPEIRTGRTLNDQDLYIPVDHQMIFTTDASFANTSNDKIMYIDYANLTKVIVPGRFIYVD 148 GPEIRTGR D +L IP H+M+ TTD + AN + +YIDY L G++I+VD Sbjct 32771 GPEIRTGRIKGD*ELSIPHGHEMVLTTDPARANDGDIDCIYIDYEKLPHSTDVGKYIFVD 32592 Query 149 DGILSFKVLQIIDESNLRVQAVNSGYIASHKGVNLPNTDVDLPPLSAKDMKDLQFGVRNG 208 DG L F+V+ I +RV A NS I+++KGVNLP T V+LP LS +D +DLQFG+ N Sbjct 32591 DGQLRFQVVSI-QGREVRV*AQNSWKISNNKGVNLPMTTVELPALSERDKRDLQFGIEND 32415 Query 209 IHIVFASFIRTSEDVLSIRKALGS----EGQDIKIISKIENQQGLDNFDEILEVTDGVMI 264 + ++FASFIR ED+L IR+ L + G+DIKII+KIE+ +GLDNFDEIL+VTDG+M+ Sbjct 32414 VDMIFASFIRKKEDILEIRQILDTFMNGRGKDIKIIAKIESHEGLDNFDEILQVTDGIMV 32235 Query 265 ARGDLGIEILAPEVLAIQKKLIAKCNLAGKPVICATQMLDSMTHNPRPTRAEVSDVGNAV 324 ARGDLGIEI A +V QK +IAKCN GKP+ICATQML++MT+NPRPTRAEVSDV NAV Sbjct 32234 ARGDLGIEIAAEKVFIAQKMMIAKCNAVGKPIICATQMLETMTYNPRPTRAEVSDVANAV 32055 Query 325 LDGADCVMLSGETAKGDYPVNAVNIMAATALIAESTIAHLALYDDLRDATPKPtsttetv 384 LDGADCVMLSGETAKG YP+ V++M AES + + L+++LRD TPKPTSTTE + Sbjct 32054 LDGADCVMLSGETAKGQYPLETVDMMNRLCKEAESVLFYPQLFNELRDVTPKPTSTTEAI 31875 Query 385 aaaataaILEQDGKAIVVLSTTGNTARLLSKYRPSCPIILVTRHARTARIAHLYRGVFPF 444 A++A A E AI VL+TTG +ARL+SKYRP P+I VTR+A+ AR HLYRG +PF Sbjct 31874 ASSAVNAAEE*GAAAICVLTTTGESARLISKYRPHIPVITVTRNAKVAR*CHLYRGCYPF 31695 Query 445 LYEPKRL-----------------DDWGEDVHRRLKFGVEMARSFGMVDNGDTVVSIQGF 487 +YE R D W EDV R + ++ + GM+ GD +V++QG+ Sbjct 31694 VYEKARKQGSESAPASPNS*SMVHDQWQEDVDERFFWAMKEGKKLGMLKAGDVIVAVQGW 31515 Query 488 KGGVGHSNTLRI 499 +GG G ++ +R+ Sbjct 31514 RGGAGSTSVMRM 31479 > scaffold-199 Length=1112851 Score = 31.2 bits (69), Expect = 1.5, Method: Compositional matrix adjust. Identities = 19/48 (40%), Positives = 29/48 (60%), Gaps = 7/48 (15%) Frame = -3 Query 423 ILVTRHARTARIA-HLYRGVFPFLYEPKRLDDWG-----EDVHRRLKF 464 L+ R+ RT IA ++YR V PF++E + DWG D+++ LKF Sbjct 287558 FLMRRYGRTNLIAFNVYR-VLPFVWEIRNFIDWGLSDTCLDLYQWLKF 287418 > scaffold-212 Length=372105 Score = 30.8 bits (68), Expect = 1.8, Method: Compositional matrix adjust. Identities = 19/48 (40%), Positives = 29/48 (60%), Gaps = 7/48 (15%) Frame = +2 Query 423 ILVTRHARTARIA-HLYRGVFPFLYEPKRLDDWG-----EDVHRRLKF 464 L+ R+ RT IA ++YR V PF++E + DWG D+++ LKF Sbjct 260681 FLMRRYGRTNLIAFNVYR-VLPFVWEIRNFIDWGLSDTCLDLYQWLKF 260821 > scaffold-100 Length=762135 Score = 29.6 bits (65), Expect = 4.0, Method: Compositional matrix adjust. Identities = 16/57 (28%), Positives = 29/57 (51%), Gaps = 4/57 (7%) Frame = +1 Query 104 YIPVDHQMIFTTDASFANTSNDKIMYIDYANLTKVIVPGRFIY--VDDGILSFKVLQ 158 + P + +FT AS + ++ + +Y + V PG FIY ++G S++VL Sbjct 105901 FSPASAENVFTVGAS--DILDNPAFFSNYGSCVNVFSPGHFIYSSWNEGPKSYQVLS 106065 > scaffold-287 Length=687983 Score = 29.3 bits (64), Expect = 5.0, Method: Compositional matrix adjust. Identities = 24/65 (37%), Positives = 32/65 (49%), Gaps = 9/65 (14%) Frame = +2 Query 134 NLTKVIVPGRFIYVDDGILSF------KVLQIIDESNLRVQAVNSG--YIAS-HKGVNLP 184 NLT + +YVD GIL+F +L+II ES +R Y+ S K V L Sbjct 345143 NLTNTWSVCKRVYVDGGILAFWTGNGVNILKIIPESAVRFYVYERAKRYMVSVRKPVALI 345322 Query 185 NTDVD 189 + DVD Sbjct 345323 SADVD 345337 > scaffold-17 Length=2125590 Score = 29.3 bits (64), Expect = 6.4, Method: Compositional matrix adjust. Identities = 12/33 (36%), Positives = 17/33 (52%), Gaps = 0/33 (0%) Frame = -1 Query 77 PGRPLAIALDTKGPEIRTGRTLNDQDLYIPVDH 109 P +PL + T GPE R + + +LY P H Sbjct 210483 PSQPLPASCSTPGPEEREA*SQHHHELYNPKAH 210385 Lambda K H a alpha 0.320 0.137 0.391 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 3149925380 Database: X5.fasta Posted date: Nov 7, 2021 11:44 PM Number of letters in database: 23,962,143 Number of sequences in database: 1,868 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 13 Window for multiple hits: 40