AatI	NEBC13653	Type II	R	AGGCCT	274	-	-	-	-	no	-
AatII	U65398	Type II	R	GACGTC	345	-	AAC44856.1	P70725	1498744	no	-
AbrI	X62690	Type II	R	CTCGAG	222	-	CAA44567.1	P26919	38661	no	-
AccII	NEBC14933	Type II	R	CGCG	354	-	-	-	-	no	-
Alw26I	AF458984	Type II	R	GTCTC	473	-	AAM09643.1	Q8RNY2	20068991	no	-
ApaI	HQ327698	Type II	R	GGGCCC	355	-	ADO24182.1	E3VXA1	308229533	no	-
ApaI	HQ327698	Type II	R	GGGCCC	355	-	ADO24182.1	E3VXA1	308229533	no	-
AplI	AP011615	Type II	R	CTGCAG	324	NIES39_K04640	BAI92109.1	D4ZX34	291569837	no	-
AspBHII	AM406670	Type II	R	RGGWCCY	367	-	-	-	-	no	-
AvaII	BA000019	Type II	R	GGWCC	230	-	BAB72890.1	Q8YYB7	17130279	no	-
BalI	D82028	Type II	R	TGGCCA	260	-	BAA11515.1	P71102	1620877	no	-
BbrUII	CP000303	Type II	R	GTCGAC	695	Bbr_1120	ABE95798.1	C1J0P6	339479330	no	-
BbrUIII	CP000303	Type II	R	CTGCAG	315	Bbr_1118	ABE95796.1	C1J0P8	339479328	no	-
BceSII	AE017194	Type II	R	GGWCC	264	BCE_4604	AAS43505.1	Q72ZR4	42739579	no	-
BcoDI	NZ_ABIY02000022	Type II	R	GTCTC	471	BACCOP_00224	ZP_03008383.1	-	189459598	no	-
BfaIA	HQ446228	Type II	R	CTAG	205	-	ADQ20485.1	E5LG97	311977224	no	BfaI
BfaIB	HQ446228	Type II	R	CTAG	196	-	ADQ20486.1	E5LG98	311977225	no	BfaI
BfuAII	NEBC7814	Type II	R	GCATGC	251	-	-	-	-	no	-
BfuAII	NEBC7814	Type II	R	GCATGC	251	-	-	-	-	no	-
BhaII	BA000004	Type II	R	GGCC	317	-	BAB07228.1	Q9K764	-	no	-
BsaHI	EU386360	Type II	R	GRCGYC	370	-	ABY86225.1	B0LX59	166237007	no	-
BsaJI	HQ446236	Type II	R	CCNNGG	307	-	ADQ20508.1	E5LGC0	311977255	no	-
BsmAI	AY364324	Type II	R	GTCTC	465	-	AAQ72363.1	Q6UQ64	34451615	no	-
BsmBI	AY452068	Type II	R	CGTCTC	530	-	AAS09914.1	Q69B20	41584551	no	-
BspRI	X15758	Type II	R	GGCC	304	-	CBE66553.1	C9IY17	258593907	no	-
BssHII	NEBC14846	Type II	R	GCGCGC	417	-	-	-	-	no	-
BssHII	NEBC14846	Type II	R	GCGCGC	417	-	-	-	-	no	-
BstFI	NEBC15050	Type II	R	AAGCTT	318	-	-	-	-	no	-
BstVI	L07643	Type II	R	CTCGAG	224	-	AAA22281.1	P43421	142619	no	-
BstZ1II	AY789018	Type II	R	AAGCTT	318	-	AAX14642.1	Q32WE8	60202513	no	-
BsuBI	L01541	Type II	R	CTGCAG	316	-	AAA18170.1	P33562	143055	no	-
BsuMIA	AL009126	Type II	R	CTCGAG	313	BSU_06090	CAB12428.1	O35025	-	no	BsuMI
BsuMIB	AL009126	Type II	R	CTCGAG	343	BSU_06100	CAB12429.1	O34885	-	no	BsuMI
BsuMIC	AL009126	Type II	R	CTCGAG	465	BSU_06110	CAB12430.1	O34303	-	no	BsuMI
BsuRI	X02988	Type II	R	GGCC	576	-	CAA26730.1	P06529	40246	no	-
CbeI	CP001393	Type II	R	GGCC	326	Athe_2438	ACM61506.1	B9MNH5	222457244	no	-
Cce743II	CP002160	Type II	R	CCWGG	857	Clocel_4006	ADL53669.1	D9SLN2	302579657	no	-
CchI	CP000108	Type II	R	CTAG	197	Cag_1100	ABB28362.1	Q3ARL3	78171266	no	-
Cfr42I	EU262735	Type II	R	CCGCGG	216	-	ABY83632.1	B0LL99	166163614	no	-
CpaAI	CP009267	Type II	R	CGCG	296	CPAST_c05920	AJA46692.1	-	-	no	-
Csp231I	AY787793	Type II	R	AAGCTT	313	-	AAX19733.1	Q32WH3	60418613	no	-
Csp68KI	CP000806	Type II	R	GGWCC	233	cce_3474	ACB52822.1	B1WZS3	171699841	no	-
Csp68KII	CP000806	Type II	R	TTCGAA	219	cce_2281	ACB51631.1	B1WPR1	171698650	no	-
Csp68KVI	CP000806	Type II	R	CGCG	387	cce_2550	ACB51898.1	B1WSB2	171698917	no	-
CviAII	JF411744	Type II	R	CATG	342	-	AAC97063.2	O41016	338221978	no	-
DsaV	U10528	Type II	R	CCNGG	119	-	AAA86045.1	P50184	794254	no	-
EaeI	AF039582	Type II	R	YGGCCR	315	-	AAB95338.1	O52511	2760956	no	-
EagI	EU371940	Type II	R	CGGCCG	301	-	ABY76238.1	B0LT74	165973117	no	-
EagI	EU371940	Type II	R	CGGCCG	301	-	ABY76238.1	B0LT74	165973117	no	-
Ecl18kI	Y16897	Type II	R	CCNGG	305	-	CAA76527.1	O87963	3421012	no	-
Eco128I	AY618907	Type II	R	CCWGG	365	-	AAT40240.1	Q6IVW7	48093756	no	-
Eco1524I	EU863203	Type II	R	AGGCCT	272	-	-	-	-	no	-
Eco29kI	AJ001708	Type II	R	CCGCGG	214	-	CAA04943.1	Q46944	2660512	no	-
Eco31I	AF458982	Type II	R	GGTCTC	579	-	AAM09638.2	Q8RNY7	56788324	no	-
Eco47I	X82105	Type II	R	GGWCC	230	-	CAA57630.1	P50194	558572	no	-
Eco47IA	GQ231553	Type II	R	GGWCC	133	-	-	-	-	no	Eco47I
Eco47IB	GQ231553	Type II	R	GGWCC	119	-	-	-	-	no	Eco47I
EcoGIII	AFST01000004	Type II	R	CTGCAG	317	C22711_2126	EGT68097.1	-	341918483	no	-
EcoHK31I	X82231	Type II	R	YGGCCR	318	-	CAA57707.1	O87741	3660495	no	-
EcoRII	NEBM64	Type II	R	CCWGG	402	-	CAA39344.1	P14633	41323	no	-
EcoRV	AF251289	Type II	R	GATATC	245	-	AAG18131.1	Q7BLB3	10504551	no	-
EcoVIII	AF158026	Type II	R	AAGCTT	307	-	AAF71176.1	Q9JP70	7960014	no	-
EsaWC1I	AY588130	Type II	R	GGCC	129	-	AAS99731.1	Q6PPG6	46518734	no	-
Esp3I	AF458983	Type II	R	CGTCTC	533	-	AAM09641.1	Q8RNY4	20068988	no	-
FnuDI	AF051374	Type II	R	GGCC	284	-	AAC05694.1	O68582	2961230	no	-
FseI	JF323049	Type II	R	GGCCGGCC	219	-	ADX97304.1	F1KC51	323650452	no	-
FspI	JF323052	Type II	R	TGCGCA	249	-	ADX97311.1	F1KC58	-	no	-
FssI	CP002158	Type II	R	GGWCC	234	FSU_1381	ADL26476.1	C9RP75	302327275	no	-
HaeII	AF019752	Type II	R	RGCGCY	352	-	AAB70830.1	O30869	2425088	no	-
HaeIII	AF051375	Type II	R	GGCC	318	-	AAC05697.1	O68584	2961234	no	-
HgiBI	X55137	Type II	R	GGWCC	274	-	CAA38928.1	P25257	43479	no	-
HgiCII	X55139	Type II	R	GGWCC	273	-	CAA38936.1	P25259	43465	no	-
HgiDI	X55140	Type II	R	GRCGYC	359	-	CAA38937.1	P24599	43481	no	-
HgiDII	X55141	Type II	R	GTCGAC	611	-	CAA38942.1	P25280	48774	no	-
HgiEI	X55142	Type II	R	GGWCC	274	-	CAA38945.1	P25260	43472	no	-
HgiGI	X55143	Type II	R	GRCGYC	335	-	-	-	-	no	-
HhaI	CP038817	Type II	R	GCGC	258	E5Q53_07170	QEN11223.1	A0A5C1Q	-	no	-
HinP1I	CS808160	Type II	R	GCGC	247	-	-	-	-	no	-
HindIII	L42023	Type II	R	AAGCTT	300	HI_1393	AAC23040.1	P43870	1574230	no	-
Hpy99I	AE001439	Type II	R	CGWCG	190	jhp_0755	AAD06326.1	Q9ZL26	4155311	no	-
Hpy99III	CP011330	Type II	R	GCGC	250	YH61_07285	AKE82403.1	-	816208592	no	-
Hpy99IV	AE001439	Type II	R	CCNNGG	290	jhp_0630	AAD06207.1	Q9ZLF0	4155181	no	-
HpyCH4I	AF459446	Type II	R	CATG	228	-	AAL59391.1	Q8VQ95	18092657	no	-
HsoI	CP000947	Type II	R	GCGC	247	HSM_0802	ACA32475.1	B0USN9	168827104	no	-
KasI	JF323050	Type II	R	GGCGCC	285	-	ADX97306.1	F1KC53	323650455	no	-
KasI	JF323050	Type II	R	GGCGCC	285	-	ADX97306.1	F1KC53	323650455	no	-
Kpn2kI	AF300473	Type II	R	CCNGG	305	-	AAG38100.1	Q71SG8	11559817	no	-
LlaCI	HQ646604	Type II	R	AAGCTT	332	-	AEV59860.1	G9BNL2	359755276	no	-
LlaMI	AF487827	Type II	R	CCNGG	271	-	AAM03111.1	Q8RMX1	19909095	no	-
McaTI	AE017282	Type II	R	GCGCGC	340	MCA1617	AAU92380.1	Q607Y4	53758089	no	-
McaTI	AE017282	Type II	R	GCGCGC	340	MCA1617	AAU92380.1	Q607Y4	53758089	no	-
MjaI	L77117	Type II	R	CTAG	222	MJ_0984	AAB98987.1	Q58391	1591646	no	-
MluI	JF323053	Type II	R	ACGCGT	276	-	AEN71951.1	G3EST6	345106999	no	-
MscI	JF432060	Type II	R	TGGCCA	261	-	ADZ31426.1	F1KM51	325564204	no	-
MthTI	X68366	Type II	R	GGCC	281	-	CAA48435.1	P29565	44640	no	-
MthZI	X68367	Type II	R	CTAG	202	-	CAA48446.1	P29566	809724	no	-
MunI	X76192	Type II	R	CAATTG	202	-	CAA53788.1	P43642	431930	no	-
MvaI	AF472612	Type II	R	CCWGG	246	-	AAM03024.2	Q8RNV5	119392963	no	-
NcoI	AF068761	Type II	R	CCATGG	288	-	AAC23515.1	O85489	3219719	no	-
NgoAI	AE004969	Type II	R	RGCGCY	350	NGO_1992	AAW90600.1	Q5F5D7	59719195	no	-
NgoAII	AE004969	Type II	R	GGCC	278	-	-	-	-	no	-
NgoAIII	AE004969	Type II	R	CCGCGG	213	-	-	-	-	no	-
NgoBI	U42459	Type II	R	RGCGCY	350	-	AAB03207.2	Q50973	5924405	no	-
NgoBV	U43735	Type II	R	GGNNCC	175	-	AAA86269.1	Q50975	1165243	no	-
NgoMI	CP003909	Type II	R	RGCGCY	350	NGFG_01893	EEZ48977.1	-	268584301	no	-
NgoMIII	CP003909	Type II	R	CCGCGG	213	NGFG_02013	EEZ48556.1	-	268583880	no	-
NgoMV	CP003909	Type II	R	GGNNCC	243	NGFG_01122	EEZ47955.1	-	268583279	no	-
NgoPII	X52661	Type II	R	GGCC	278	-	CAA36887.1	P24617	44887	no	-
NgoSII	U42460	Type II	R	GGCC	212	-	AAB03208.1	Q79CC3	1399075	no	-
NheI	AY462277	Type II	R	GCTAGC	328	-	AAR23810.1	Q6S994	38564763	no	-
NlaIV	U06074	Type II	R	GGNNCC	243	-	AAA53238.1	P50183	476228	no	-
Nno22aORF54440P	CP006850	Type II	R	GTCGAC	307	NONO_c54450	AHH20225.1	W5TLJ5	576087835	yes	-
NspHI	AF056037	Type II	R	RCATGY	244	-	AAC97193.1	Q9ZHP0	4033741	no	-
NspI	AF056036	Type II	R	RCATGY	244	-	AAC97191.1	Q9ZHP2	4033738	no	-
NspV	D14719	Type II	R	TTCGAA	220	-	BAA03538.1	P35677	442473	no	-
PaeR7I	X03274	Type II	R	CTCGAG	246	-	CAA27026.1	P05104	45387	no	-
Pam7686I	GQ468938	Type II	R	CCATGG	308	-	ADF47150.1	E7BLE5	294869158	no	-
PciI	JX481785	Type II	R	ACATGT	257	-	AFV09321.1	K4LNB0	409034609	no	-
PciI	JX481785	Type II	R	ACATGT	257	-	AFV09321.1	K4LNB0	409034609	no	-