MAST version 4.3.0 (Release date: Sat Sep 26 01:51:56 PDT 2009)

For further information on how to interpret these results or to get a copy of the MAST software please access http://meme.nbcr.net.

If you use this program in your research, please cite:

Timothy L. Bailey and Michael Gribskov, "Combining evidence using p-values: application to sequence homology searches", Bioinformatics, 14(48-54), 1998.

	DATABASE ./seed.fasta (peptide)
	Last updated on Sun May 26 16:57:51 2013
	Database contains 10 sequences, 2022 residues

	MOTIFS ./meme.txt (peptide)
	MOTIF WIDTH BEST POSSIBLE MATCH
	----- ----- -------------------
	  1    29   HWENINQRMPLCRFGYFHMYNNYYVHWEM
	  2    41   CHEKDCYDGLFDMKHDSQYVTISYNYFHHHWKVMLWGHSDS
	  3    50   SGINSRMGAKIKVEQNYFENMKDPLMTFYTQQIGYWQVRDNMFDNVTWEE

	PAIRWISE MOTIF CORRELATIONS:
	MOTIF     1     2
	----- ----- -----
	   2   0.10
	   3   0.20  0.08
	No overly similar pairs (correlation > 0.60) found.

	Random model letter frequencies (from non-redundant database):
	A 0.073 C 0.018 D 0.052 E 0.062 F 0.040 G 0.069 H 0.022 I 0.056 K 0.058 
	L 0.092 M 0.023 N 0.046 P 0.051 Q 0.041 R 0.052 S 0.074 T 0.059 V 0.064 
	W 0.013 Y 0.033 

• Each of the following 10 sequences has E-value less than 10.
• The E-value of a sequence is the expected number of sequences in a random database of the same size that would match the motifs as well as the sequence does and is equal to the combined p-value of the sequence times the number of sequences in the database.
• The combined p-value of a sequence measures the strength of the match of the sequence to all the motifs and is calculated by
  • finding the score of the single best match of each motif to the sequence (best matches may overlap),
  • calculating the sequence p-value of each score,
  • forming the product of the p-values,
  • taking the p-value of the product.
• The sequence p-value of a score is defined as the probability of a random sequence of the same length containing some match with as good or better a score.
• The score for the match of a position in a sequence to a motif is computed by by summing the appropriate entry from each column of the position-dependent scoring matrix that represents the motif.
• Sequences shorter than one or more of the motifs are skipped.
• The table is sorted by increasing E-value.

• The ordering and spacing of all non-overlapping motif occurrences are shown for each high-scoring sequence listed in Section I.
• A motif occurrence is defined as a position in the sequence whose match to the motif has POSITION p-value less than 0.0001.
• The POSITION p-value of a match is the probability of a single random subsequence of the length of the motif scoring at least as well as the observed match.
• For each sequence, all motif occurrences are shown unless there are overlaps. In that case, a motif occurrence is shown only if its p-value is less than the product of the p-values of the other (lower-numbered) motif occurrences that it overlaps.
• The table also shows the E-value of each sequence.
• Spacers and motif occurences are indicated by
• Spacers and motif occurences are indicated by
• Spacers and motif occurences are indicated by

  • n

    occurrence of motif `n' with p-value less than 0.0001.

  • 1

    Sequences longer than 1000 are not shown to scale and are indicated by thicker lines.

• The positions and p-values of the non-overlapping motif occurrences are shown above the actual sequence for each of the high-scoring sequences from Section I.
• A motif occurrence is defined as a position in the sequence whose match to the motif has POSITION p-value less than 0.0001 as defined in Section II.
• For each sequence, the first line specifies the name of the sequence.
• The second (and possibly more) lines give a description of the sequence.
• Following the description line(s) is a line giving the length, combined p-value, and E-value of the sequence as defined in Section I.
• The next line reproduces the motif diagram from Section II.
• The entire sequence is printed on the following lines.
• Motif occurrences are indicated directly above their positions in the sequence on lines showing
  • the motif number of the occurrence,
  • the position p-value of the occurrence,
  • the best possible match to the motif, and
  • columns whose match to the motif has a positive score (indicated by a plus sign).

             [2]                                                     [1]
             1.8e-38                                                 1.7e-29
             CHEKDCYDGLFDMKHDSQYVTISYNYFHHHWKVMLWGHSDS               HWENINQRMPL
             +++++++++++++++++++++++++++++++++++++++++               +++++++++++
76   GKNIWVDHCSLSNCHDGLIDAIHGSTAITISNNYFTHHDKVMLLGHSDSFTQDKGMQVTVAFNHFGEGLVQRMPR

     
     
     CRFGYFHMYNNYYVHWEM
     ++++++++++++++++++
151  CRHGYFHVVNNDYTHWEMYAIGGSAAPTINSQGNRF

             [2]                                                     [1]
             2.3e-15                                                 6.8e-22
             CHEKDCYDGLFDMKHDSQYVTISYNYFHHHWKVMLWGHSDS               HWENINQRMPL
              + ++  ++++++  +++ +++++  ++++ ++++++  +                +++++++++++
76   SHDIWIDHISMSRATDGLIDAVAGSTNITISNCHFTDHEKVMLFGANDHAEEDRGMKITLAYNHFGKRLDQRMPR

     
     
     CRFGYFHMYNNYYVHWEM
     ++++ ++ +++++++++
151  CRFGFFHLVNNDYTHWERYAIGGSSGATIISQGNRF

                                                   [2]
                                                   1.8e-19
                                                   CHEKDCYDGLFDMKHDSQYVTISYNYFHH
                                                   + ++  +++ +++++++++++++ + +
76   APHYEEGDGWNAEWDAAVIDNSTRVWVDHVTISDGSFTDDKYTTKNGEKYVQHDGALDIKKGSDYVTISSSRFEL

                                 [1]
                                 2.5e-12
     HWKVMLWGHSDS                HWENINQRMPLCRFGYFHMYNNYYVHWEM
     ++++ +++++++                 ++++  ++++ +++  + +++ + +  +
151  HDKTILIGHSDSNGSQDSGKLRVTFHNNVFDRVTERTPRVRFGSIHAYNNVYLGDVKNSVYPYLYSFGLGTSGTI

               [2]                                                     [1]
               4.1e-18                                                 5.8e-11
               CHEKDCYDGLFDMKHDSQYVTISYNYFHHHWKVMLWGHSDS               HWENINQRM
                + ++  +++++ +  ++ ++++++ ++++ +++++++ +                ++++  +++
76   RTATNIWIDHNSFSNSSDGLVDVTLSSTGVTISNNLFFNHHKVMLLGHDDAYSDDKSMKVTVAFNQFGPNCGQRM

     
     
     PLCRFGYFHMYNNYYVHWEM
     ++ + + +++ ++ ++ +
151  PRARYGLVHVANNNYDPWTIYAIGGSSNPTILSEGNSF

            [2]                                                     [1]
            7.7e-15                                                 1.3e-13
            CHEKDCYDGLFDMKHDSQYVTISYNYFHHHWKVMLWGHSDS               HWENINQRMPLC
            + ++   +++++   +++ +++++ +++++ ++ +++ ++                +   +  +++++
76   SDIWIDHCTLSKSFDGLVDVNWGSTGVTISNCKFTHHEKAVLLGASDTHFQDLKMHVTLAYNIFTNTVHERMPRC

     
     
     RFGYFHMYNNYYVHWEM
     +++ + +++++++ +++
151  RFGFFQIVNNFYDRWDKYAIGGSSNPTILSQGNKF

                                        [2]
                                        1.0e-09
                                        CHEKDCYDGLFDMKHDSQYVTISYNYFHHHWKVMLWGHSD
                                                  +++++ ++ +++++++ +   ++++ +++
76   PGGAKDGDAIRIDNSPNVWIDHNEIFAKNFECAGTPDNDTTFESAVDIKKASTNVTVSYNFIHGVKKVGLSGSSN

                 [1]
                 2.7e-15
     S           HWENINQRMPLCRFGYFHMYNNYYVHWEM
                  +  +++++++ ++++++ +++ +++++
151  TDTGRNLTYHHNIYSDVNSRLPLQRGGQVHAYNNLYDGIKSSGFNVRQKGIALIESNWF

                                                       [2]
                                                       6.8e-16
                                                       CHEKDCYDGLFDMKHDSQYVTISYN
                                                       +  +  +++ +++++++++++++++
76   IDVEPHYEKGDGWNAEWDAMNITNGAHHVWIDHVTISDGNFTDDMYTTKDGETYVQHDGALDIKRGSDYVTISNS

                                     [1]
                                     7.8e-08
     YFHHHWKVMLWGHSDS                HWENINQRMPLCRFGYFHMYNNYYVHWEM
         +++++++++ +                  + ++  ++++ + +  + +++ + +  +
151  LIDQHDKTMLIGHNDTNSAQDKGKLHVTLFNNVFNRVTERAPRVRYGSIHSFNNVFKGDAKDPVYRYQYSFGIGT

                                        [2]
                                        4.4e-10
                                        CHEKDCYDGLFDMKHDSQYVTISYNYFHHHWKVMLWGHSD
                                                  +++++ ++ +++++++ +   ++++ +++
76   PGGAKDGDMIRVDDSPNVWVDHNELFAANHECDGTPDNDTTFESAVDIKGASNTVTVSYNYIHGVKKVGLDGSSS

                 [1]
                 1.6e-11
     S           HWENINQRMPLCRFGYFHMYNNYYVHWEM
     +           ++  ++ ++++ +++ ++ +++ ++++
151  SDTGRNITYHHNYYNDVNARLPLQRGGLVHAYNNLYTNITGSGLNVRQNGQALIENNWF

                    [2]                                                        [
                    1.5e-06                                                    1
                    CHEKDCYDGLFDMKHDSQYVTISYNYFHHHWKVMLWGHSDS                  H
                          ++ ++ +   +++ ++++ ++  +  +  +++                     +
76   SKYWIDHCSFVGHSWSTTDGSEDKLLYIGEKADYATISNCFFGSHKYGLIFGHPADDNNAAFNGYPRLTLCHNRF

     1]
     .9e-06
     WENINQRMPLCRFGYFHMYNNYYVHWEM
         +    + + +++++++++  + +
151  DNMEVRAPGLMRYGYFHVYNNYINKFHLGFTLAQNANILSESNYF

                     [2]
                     3.0e-06
                     CHEKDCYDGLFDMKHDSQYVTISYNYFHHHWKVMLWGHSDS
                            + ++ +   ++++++++  + ++  +  +++
76   GKGYWVDHCSWPGHTWSDNDGSLDKLIYIGEKADYITISNCLFSNHKYGCIFGHPADDNNSAYNGYPRLTICHNY

     [1]
     3.7e-06
     HWENINQRMPLCRFGYFHMYNNYYVHWEM
     +    +    + + +++++++++  + +
151  YENIQVRAPGLMRYGYFHVFNNYVNKFQLAFTVAQNANVISERNVF

CPU: kodomo.fbb.msu.ru
Time 0.016001 secs.

mast ./meme.txt -d ./seed.fasta -ev 10.000000 -mt 0.000100