FastQCFastQC Report
Mon 16 Dec 2019
SRR4240360.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4240360.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8254632
Sequences flagged as poor quality0
Sequence length36
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA289950.35125733042975144No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN158800.19237683763491817No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN154040.18661037827004281No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN134140.16250270151352597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTCG40600.026.8876769
AGAGCTC42250.025.9303258
GCCGTCT38300.025.49621219
CGTATGC41700.025.03718614
TGCCGTC39500.024.8275818
TATGCCG41150.024.7746716
GTATGCC42400.024.52650815
GCTCGTA44250.024.18494211
AGCTCGT44750.023.83858310
CCGTCTT41300.023.68864420
CGGAAGA48250.023.6424774
GAAGAGC47300.023.5252866
ATGCCGT42400.023.44345517
TCGGAAG51050.023.098073
TCGTATG47350.022.58150113
GGAAGAG51450.022.4453685
AAGAGCT49550.022.1015117
CGTCTTC44300.022.01605421
CTCGTAT50350.021.14314312
GTCTTCT48050.020.39647522
Produced by FastQC (version 0.11.5)