FastQCFastQC Report
Tue 17 Dec 2019
SRR4240360_trim.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4240360_trim.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7915471
Sequences flagged as poor quality0
Sequence length32-36
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180440.2279586394795711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG10350.09.9852330
GATCGGA19950.08.43888830
AGATCGG23800.07.77414730
CAGATCG11850.06.32995930
TAGATCG19200.05.99038330
CGGAAGA7355.4187694E-95.896478730
ATCGGAA20350.05.569949630
GAGATCG12300.05.28525930
Produced by FastQC (version 0.11.5)