FastQCFastQC Report
Mon 16 Dec 2019
SRR4240360_trimadapt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4240360_trimadapt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8212773
Sequences flagged as poor quality0
Sequence length1-36
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA289950.35304762471822854No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN158800.19335734714693809No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN154040.1875614971946747No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN134140.16333094802449794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG10350.09.605412530
AGATCGG25950.08.70695630
GATCGGA20900.07.783768730
CGGAAGA7800.07.145284730
GAGCTCG3752.6378135E-56.3853879
ATCGGAA21250.05.883443430
CAGATCG12550.05.88118430
GAGATCG10351.4551915E-115.23931630
Produced by FastQC (version 0.11.5)