BLASTP 2.2.10 [Oct-19-2004]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KPN2Jun2003_27 [1521 - 820] (REVERSE SENSE) 
         (234 letters)

Database: ep 
           4855 sequences; 1,534,022 total letters

Searching..........done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ULAD_ECOLI P39304 3-keto-L-gulonate-6-phosphate decarboxylase ul...    99   2e-22
SGBH_ECOLI P37678 3-keto-L-gulonate-6-phosphate decarboxylase sg...    81   8e-17

>ULAD_ECOLI P39304 3-keto-L-gulonate-6-phosphate decarboxylase ulaD
           (EC 4.1.1.85) (3- dehydro-L-gulonate-6-phosphate
           decarboxylase) (KGPDC) (L-ascorbate utilization protein
           D).
          Length = 216

 Score = 99.4 bits (246), Expect = 2e-22
 Identities = 66/209 (31%), Positives = 104/209 (49%), Gaps = 3/209 (1%)

Query: 26  LQLALDELTLPEALXXXXXXXXXXXXXEVGTPFLIREGVNAIKAIKEKYPHKEVLADAKI 85
           LQ+ALD  T+  A              EVGT   + EGV A++ +K  YPHK VLADAKI
Sbjct: 6   LQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGVRAVRDLKALYPHKIVLADAKI 65

Query: 86  MDGGHFESQMLFDAGADYVTVLGVTDVLTIQSCIRAAKEAGKQVVVDMICVDDLPARVRL 145
            D G   S+M F+A AD+VTV+   D+ T +  +  AKE    V +++        + + 
Sbjct: 66  ADAGKILSRMCFEANADWVTVICCADINTAKGALDVAKEFNGDVQIELTGYWTW-EQAQQ 124

Query: 146 LEEAGADMLAVHTGTDQQAAGRKPIDDLITMLK--ARRKARIAVAGGISSQTVKDYALLG 203
             +AG   +  H   D QAAG    +  IT +K  +    ++ V GG++ + +  +  + 
Sbjct: 125 WRDAGIGQVVYHRSRDAQAAGVAWGEADITAIKRLSDMGFKVTVTGGLALEDLPLFKGIP 184

Query: 204 PDVVIVGSAITHSADPADEARKISQVLLQ 232
             V I G +I  +A P + AR+  + + +
Sbjct: 185 IHVFIAGRSIRDAASPVEAARQFKRSIAE 213


>SGBH_ECOLI P37678 3-keto-L-gulonate-6-phosphate decarboxylase sgbH
           (EC 4.1.1.85) (3- dehydro-L-gulonate-6-phosphate
           decarboxylase) (KGPDC).
          Length = 220

 Score = 80.9 bits (198), Expect = 8e-17
 Identities = 55/203 (27%), Positives = 97/203 (47%), Gaps = 9/203 (4%)

Query: 26  LQLALDELTLPEALXXXXXXXXXXXXXEVGTPFLIREGVNAIKAIKEKYPHKEVLADAKI 85
           LQLALD  +L  A              E GT   + EG+ A+KA++E+ P K ++AD K+
Sbjct: 6   LQLALDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGLGAVKALREQCPDKIIVADWKV 65

Query: 86  MDGGHFESQMLFDAGADYVTVLGVTDVLTIQSCIRAAKEAGKQVVVDMI---CVDDLPAR 142
            D G   +Q  F AGA+++T++    + T++     A+  G ++ +++     +DD    
Sbjct: 66  ADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMAQRCGGEIQIELFGNWTLDD---- 121

Query: 143 VRLLEEAGADMLAVHTGTDQQAAGRKPIDDLITMLKARRK--ARIAVAGGISSQTVKDYA 200
            R     G      H G D QA+G++  +  +  +KA       +++ GGI+   +  + 
Sbjct: 122 ARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALSDIGLELSITGGITPADLPLFK 181

Query: 201 LLGPDVVIVGSAITHSADPADEA 223
            +     I G A+  +A+PA  A
Sbjct: 182 DIRVKAFIAGRALAGAANPAQVA 204

  Database: ep
    Posted date:  Oct 3, 2006 10:20 PM
  Number of letters in database: 1,534,022
  Number of sequences in database:  4855
  
Lambda     K      H
   0.320    0.134    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 604,737
Number of Sequences: 4855
Number of extensions: 22649
Number of successful extensions: 92
Number of sequences better than 1.0e-03: 3
Number of HSP's better than  0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 88
Number of HSP's gapped (non-prelim): 3
length of query: 234
length of database: 1,534,022
effective HSP length: 82
effective length of query: 152
effective length of database: 1,135,912
effective search space: 172658624
effective search space used: 172658624
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 85 (37.4 bits)