ID   DNE1_CHLRE              Reviewed;         163 AA.
AC   P05725;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   31-OCT-2006, entry version 54.
DE   DNA endonuclease I-CreI (EC 3.1.-.-) (23S rRNA intron protein).
OS   Chlamydomonas reinhardtii.
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; Chlorophyceae;
OC   Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX   NCBI_TaxID=3055;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=85215526; PubMed=4000933;
RA   Rochaix J.-D., Rahire M., Michel F.;
RT   "The chloroplast ribosomal intron of Chlamydomonas reinhardtii codes
RT   for a polypeptide related to mitochondrial maturases.";
RL   Nucleic Acids Res. 13:975-984(1985).
RN   [2]
RP   CHARACTERIZATION.
RX   MEDLINE=93012993; PubMed=1398106; DOI=10.1016/0378-1119(92)90278-W;
RA   Thompson A.J., Yuan X., Kudlicki W., Herin D.L.;
RT   "Cleavage and recognition pattern of a double-strand-specific
RT   endonuclease (I-CreI) encoded by the chloroplast 23S rRNA intron of
RT   Chlamydomonas reinhardtii.";
RL   Gene 119:247-251(1992).
RN   [3]
RP   CHARACTERIZATION.
RX   MEDLINE=93173120; PubMed=8437585; DOI=10.1007/BF00277141;
RA   Duerrenberger F., Rochaix J.-D.;
RT   "Characterization of the cleavage site and the recognition sequence of
RT   the I-CreI DNA endonuclease encoded by the chloroplast ribosomal
RT   intron of Chlamydomonas reinhardtii.";
RL   Mol. Gen. Genet. 236:409-414(1993).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
RX   MEDLINE=97331323; PubMed=9187655;
RA   Heath P.J., Stephens K.M., Monnat R.J. Jr., Stoddard B.L.;
RT   "The structure of I-CreI, a group I intron-encoded homing
RT   endonuclease.";
RL   Nat. Struct. Biol. 4:468-476(1997).
CC   -!- FUNCTION: Endonuclease involved in group i intron homing.
CC       Recognizes and cleaves a 19-24 bp palindromic DNA site.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Plastid; chloroplast.
CC   -!- SIMILARITY: Belongs to the LAGLIDADG endonuclease family.
DR   EMBL; X01977; CAA26008.1; -; Genomic_DNA.
DR   PIR; A23091; A23091.
DR   PDB; 1AF5; X-ray; @=4-138.
DR   PDB; 1BP7; X-ray; A/B/C/D=2-153.
DR   PDB; 1G9Y; X-ray; A/B=2-153.
DR   PDB; 1G9Z; X-ray; A/B=2-153.
DR   PDB; 1MOW; X-ray; A/D/G/J=-.
DR   PDB; 1N3E; X-ray; A/B/G/H=1-163.
DR   PDB; 1N3F; X-ray; A/B/G/H=1-163.
DR   PDB; 1T9I; X-ray; A/B=1-163.
DR   PDB; 1T9J; X-ray; A/B=1-163.
DR   PDB; 1U0C; X-ray; A/B=1-163.
DR   PDB; 1U0D; X-ray; A/B=1-163.
DR   REBASE; 2540; I-CreI.
DR   LinkHub; P05725; -.
DR   InterPro; IPR001982; Endonuc_LAG/HNH.
DR   Pfam; PF00961; LAGLIDADG_1; 1.
KW   3D-structure; Chloroplast; Endonuclease; Hydrolase; Intron homing;
KW   Nuclease; Plastid.
FT   CHAIN         1    163       DNA endonuclease I-CreI.
FT                                /FTId=PRO_0000192783.
FT   ACT_SITE     20     20
FT   HELIX         7     20
FT   STRAND       21     29
FT   TURN         31     32
FT   TURN         34     35
FT   STRAND       36     48
FT   HELIX        49     51
FT   HELIX        52     62
FT   STRAND       66     70
FT   TURN         71     72
FT   STRAND       73     78
FT   HELIX        81     91
FT   HELIX        92     94
FT   STRAND       96     98
FT   HELIX        99    115
FT   TURN        116    117
FT   HELIX       119    135
FT   TURN        136    137
FT   HELIX       145    148
FT   TURN        149    150
SQ   SEQUENCE   163 AA;  18751 MW;  F3AD55D5CF2678D4 CRC64;
     MNTKYNKEFL LYLAGFVDGD GSIIAQIKPN QSYKFKHQLS LAFQVTQKTQ RRWFLDKLVD
     EIGVGYVRDR GSVSDYILSE IKPLHNFLTQ LQPFLKLKQK QANLVLKIIW RLPSAKESPD
     KFLEVCTWVD QIAALNDSKT RKTTSETVRA VLDSLSEKKK SSP
//