TBLASTN 2.9.0+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: X5.fasta
           1,868 sequences; 23,962,143 total letters



Query= sp|Q8SRB9|RS4_ENCCU 40S ribosomal protein S4 OS=Encephalitozoon
cuniculi (strain GB-M1) OX=284813 GN=RPS4 PE=1 SV=1

Length=268
                                                                      Score        E
Sequences producing significant alignments:                          (Bits)     Value

scaffold-497                                                          115        4e-29
scaffold-157                                                          113        3e-28


>scaffold-497
Length=70081

 Score = 115 bits (289),  Expect = 4e-29, Method: Compositional matrix adjust.
 Identities = 62/178 (35%), Positives = 111/178 (62%), Gaps = 3/178 (2%)
 Frame = +2

Query  80     SHFPVGLFDVVSIEKTGEHFRVLYNVHKKFHLHRISSEEASYRLTKVTK-KYENQGIPYI  138
              S+   G  DV+SIEKTGEHFR++Y+V  +F +HRI+ EEA ++L KV K +   +GIPY+
Sbjct  32141  SYLLSGCVDVISIEKTGEHFRLVYDVKGRFTIHRITDEEAKFKLCKVRKVQLGAKGIPYV  32320

Query  139    VSSCGLSIRFCDPAIDLGYTIKISNETSKVIEYIVPGPDKVIFVSRGKARGRVGVI-NTI  197
              V+  G +IR+ DP I    T+K+  ET K+++++      +  ++ G+  GR+GVI +  
Sbjct  32321  VTHDGRTIRYPDPLIKANDTVKVDLETGKIVDFVKFDTGNMCAITGGRNMGRMGVIVHRE  32500

Query  198    STQGKETVYGMTDFAGNTFSCTSKSCILIGESSENIWITLPKERGIRQSELEKINAQL  255
                 G   +  + D   +TF+    +  +IG+ ++  W++LPK++G++ + LE+ + ++
Sbjct  32501  RHLGGFDIVHIKDALDHTFATRLSNVFVIGKGNKA-WVSLPKQKGVKLTILEERDRRI  32671


 Score = 92.0 bits (227),  Expect = 5e-21, Method: Compositional matrix adjust.
 Identities = 45/92 (49%), Positives = 60/92 (65%), Gaps = 0/92 (0%)
 Frame = +1

Query  1      MPTGHRDHLKRKTAPKSWMLDKQGGTFALMPASGPHKKTECIPLGYLISRFLRYASNFKE  60
              MP G R HLKR  AP  WMLDK  GT+A  P+SGPHK  EC+PL   +   L+YA N +E
Sbjct  31252  MPRGPRKHLKRLNAPSHWMLDKMTGTWAPRPSSGPHKLRECLPLIIFLRNRLKYALNKRE  31431

Query  61     LSIILAEKNIKVNGRVRTNSHFPVGLFDVVSI  92
              +  I+  + IKV+G+VRT+++FP G    + I
Sbjct  31432  VVSIVQ*RLIKVDGKVRTDANFPAGYMGRILI  31527


>scaffold-157
Length=706690

 Score = 113 bits (282),  Expect = 3e-28, Method: Compositional matrix adjust.
 Identities = 60/171 (35%), Positives = 108/171 (63%), Gaps = 3/171 (2%)
 Frame = +3

Query  88      DVVSIEKTGEHFRVLYNVHKKFHLHRISSEEASYRLTKVTK-KYENQGIPYIVSSCGLSI  146
               DV+SIEKTGEHFR++Y+V  +F +HRI+ EEA ++L KV K +   +GIPY+V+  G +I
Sbjct  364080  DVISIEKTGEHFRLVYDVKGRFTIHRITDEEAKFKLCKVRKVQLGAKGIPYVVTHDGRTI  364259

Query  147     RFCDPAIDLGYTIKISNETSKVIEYIVPGPDKVIFVSRGKARGRVGVI-NTISTQGKETV  205
               R+ DP I    T+K+  ET K+++++      +  ++ G+  GR+GVI +     G   +
Sbjct  364260  RYPDPLIKANDTVKVDLETGKIVDFVKFDTGNMCAITGGRNMGRMGVIVHRERHLGGFDI  364439

Query  206     YGMTDFAGNTFSCTSKSCILIGESSENIWITLPKERGIRQSELEKINAQLG  256
                 + D   +TF+    +  +IG+ ++  W++LPK++G++ + LE+ + ++ 
Sbjct  364440  VHVKDALDHTFATRLSNVFVIGKGNKA-WVSLPKQKGVKLTILEERDRRIA  364589


 Score = 95.1 bits (235),  Expect = 5e-22, Method: Compositional matrix adjust.
 Identities = 45/92 (49%), Positives = 61/92 (66%), Gaps = 0/92 (0%)
 Frame = +2

Query  1       MPTGHRDHLKRKTAPKSWMLDKQGGTFALMPASGPHKKTECIPLGYLISRFLRYASNFKE  60
               MP G R HLKR  AP  WMLDK  GT+A  P+SGPHK  EC+PL   +   L+YA N +E
Sbjct  363182  MPRGPRKHLKRLNAPSHWMLDKMTGTWAPRPSSGPHKLRECLPLIIFLRNRLKYALNKRE  363361

Query  61      LSIILAEKNIKVNGRVRTNSHFPVGLFDVVSI  92
               +  I+ ++ IKV+G+VRT+++FP G    + I
Sbjct  363362  VVSIVQQRLIKVDGKVRTDANFPAGYMGRILI  363457



Lambda      K        H        a         alpha
   0.318    0.135    0.395    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 1342985116


  Database: X5.fasta
    Posted date:  Nov 10, 2021  1:45 PM
  Number of letters in database: 23,962,143
  Number of sequences in database:  1,868



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40