FastQCFastQC Report
Сб 7 дек 2019
12_chr8_trimmed.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename12_chr8_trimmed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17398
Sequences flagged as poor quality0
Sequence length50-51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCATTTTAGCATCTGGATATCTGTTTGTCCAGGTGTTCAGAAGTCTCTT1190.6839866651339235No Hit
GTTTGGATAATCTGGGCTCATGCTGGTCATTTCGAGCAGAAAATTTGTTGC730.41958845844349923No Hit
CCCTGATGCACCACAGTACTAATGCAATTCCTGAGAACTGGCCCTTTTGCT680.39084952293367053No Hit
CTGGGAGTCATTATCTGTCTCACTTTCTGGATCTCTGTAATTATCATGAAT620.35636280032187606No Hit
CCCGTTTCTTTCATTGGTAACATCAGATTGATAAACTGGCGAGTTAGCCGA610.35061501321991034No Hit
CTTCGTTTGATGAAAGGTTCTTTGTCTTCTTCAGATGCTTTAACACCACTT570.32762386481204736No Hit
GGGCTCTCTTACTGCTGATGTCATCTGGGTCTTAAGTTCACCCAGAAAAGC570.32762386481204736No Hit
GTCGCAAGTTTCAGTCCTTGGTGTTTCTGGTTTGCTCGCTCCCTGAAGCTC530.30463271640418443No Hit
CTGTAATGCCAGCACCGCGGGGCTGCTGCTCAGGACGCATTCCTGCCCCAG500.28738935509828717No Hit
CTTCAGGAGAGTGTTTCAGACTCTTTGGACTAACTCCCTCGAAATATTTTA480.2758937808943557No Hit
GGGGTTTTTGCTAGTTCAGCTCTTACATCATTGCTCCAATCCTTAAAGAGC470.27014599379238996No Hit
GCCCATCTTCTTGGTGATTTGAGTATCAAAGGTAGATTCATTTAGCTTTGT460.2643982066904242No Hit
CTGGGTCTTCAGGAGAGTGTTTCAGACTCTTTGGACTAACTCCCTCGAAAT450.25865041958845847No Hit
CCCATCTTCTTGGTGATTTGAGTATCAAAGGTAGATTCATTTAGCTTTGTA440.2529026324864927No Hit
CTCGGAGTTCTACTGGTTTTAACTTTGGCATCATCTTATAAATTATGTCCA440.2529026324864927No Hit
CTCTGGGCCACGGCCTGTAACGGGGTGATGAGGCTGCTGTGCTTGATCTGA430.24715484538452695No Hit
CCCGCGATCACCTTCTCTGAATCCTCTGAACTGGATCCAGAAAGCTCTAAG420.24140705828256123No Hit
GGGGTATAGCTCAGGCCCAGTTTGAAAGCCATCTGCAGTGCAGGAACGTAG410.23565927118059546No Hit
CTTTAAATGTAGATCCAGCTTTGTGTCAGCAAGTAGACTAATATATGTTGT390.22416369697666397No Hit
CTGGTATTTTTTTTTTCAATGCAAGTTCTCCATAGAATTTACTAAATAATT390.22416369697666397No Hit
CCATCTTCTTGGTGATTTGAGTATCAAAGGTAGATTCATTTAGCTTTGTAA370.2126681227727325No Hit
CTCCCTTGAAGTCTGGGGATCTTCTTCCATGGACTTAGTGAAGTTGCACAG360.20692033567076676No Hit
CTCAATTAATCCTAACCTGGAGGTCATGGGCACAACGCTATAGGTCCTCAG350.201172548568801No Hit
GTCCCTCTGGGCCACGGCCTGTAACGGGGTGATGAGGCTGCTGTGCTTGAT340.19542476146683527No Hit
GCCCGTTTCTTTCATTGGTAACATCAGATTGATAAACTGGCGAGTTAGCCG340.19542476146683527No Hit
CTGATAAACACGGTCGTCACCAGTGTCTGTCTGGGTGAGGAACATCTGCTT340.19542476146683527No Hit
CTAGAAACTTTTTCATGCAGTCCACATAATTATTGAACCGCGGAGTTCCTG330.18967697436486952No Hit
CTTGGTGTTTCTGGTTTGCTCGCTCCCTGAAGCTCTGGTCTAACATGCCGT320.18392918726290378No Hit
CTGGCATCTGCGTGGCTTTGCCCAACATAAACATAACCATGCTATGTAAAA320.18392918726290378No Hit
GGGGGTATAGCTCAGGCCCAGTTTGAAAGCCATCTGCAGTGCAGGAACGTA320.18392918726290378No Hit
GTTCAGAAGTTCTCGGAGTTCTACTGGTTTTAACTTTGGCATCATCTTATA320.18392918726290378No Hit
CTCTCTCAGATGGGTCTCTAGGATATCTTTGTATGGGGACATCTTTAGAGC310.17818140016093803No Hit
CTCCTCTCCAGCAGCTGATTCTCTCCTGCCATGTTCTCTGTAAATGCATCG310.17818140016093803No Hit
CTCCGGTTGAGAAATCAAATTGACTCATTTCCTCACTCAGGGTACTGTCTG310.17818140016093803No Hit
CCGCGGAGTTCCTGGAGGAAATTCCCTGGACTGCATGGGGAAGTGAGCAAC310.17818140016093803No Hit
CTCTCAGATGGGTCTCTAGGATATCTTTGTATGGGGACATCTTTAGAGCTT300.1724336130589723No Hit
CATGGTTCAAAAAATTCTGCTTGTTTCTCAGGGAGAATCTCTCTGCAAAAT300.1724336130589723No Hit
GTGCTTTCCAATGTGCCAGAACCAGAAGGGCATTCCAAGGCTTCCCCACAT300.1724336130589723No Hit
CTTGTTTCTCAGGGAGAATCTCTCTGCAAAATTCCACCAGGTTAATGAAAG300.1724336130589723No Hit
CCTTGTTGTTCGAATCCACATTTCTGATGATTCCATAAAACTGCTCCATAA290.16668582595700657No Hit
GTTCCTATGCTGCTTCTACAATGTTACATCAACTATACTTAGCTTTACTCT290.16668582595700657No Hit
GTCCGCTTATAGAGCTGGTACATGGGTGGGGCTCCCTGTCCCCCTTCTGGC290.16668582595700657No Hit
GCCTTGTTTGCTTATAAAGCTGATGAACTCCTGAATTTCTGTTAAAGCCTG280.16093803885504082No Hit
CTGGCAATAAAGGAACGAATTTATAAAAGAGTTCAATGGATTTGTGTCGAC280.16093803885504082No Hit
CTCTGAACTCCGGTTGAGAAATCAAATTGACTCATTTCCTCACTCAGGGTA280.16093803885504082No Hit
GCCACCTCTTTGCCAAATTTCACAAATAAAGCAAAGCAAGAATACTTTTCT280.16093803885504082No Hit
GTGAGGAACATCTGCTTGCAGCGCTGAATGAGCTCAACGTACATGAAGTCA280.16093803885504082No Hit
CTCCTTCAGTAGTTTCATAGCAAGTGAGAAATTGTTCTGCTTCCGGGCACT280.16093803885504082No Hit
CTCGTTTTCTAAAAGACGTGACTGTTTCAGTACGATTAGCGCCCTTATACA280.16093803885504082No Hit
GTTCCCTACATGTTGTAGAAGGATGGGAAACGAATTCCACAACGGGGTTCA280.16093803885504082No Hit
GTCCTCCTAGAGATCCAAGCATTTGTACTACTCTAATTCTTATTTCTTCTA270.15519025175307508No Hit
GGGGTTCAGAAGTTCTCGGAGTTCTACTGGTTTTAACTTTGGCATCATCTT260.14944246465110933No Hit
CTTGTTGTTCGAATCCACATTTCTGATGATTCCATAAAACTGCTCCATAAA260.14944246465110933No Hit
GTCATAGGGAGGCAGGTCATTGGCCATCACGATGCCTAGCAATTGAATCCC260.14944246465110933No Hit
CTTTGAGATAATTCCAATGCATCTAGAAACTTTTTCATGCAGTCCACATAA260.14944246465110933No Hit
GGGGCTTTGAGTCTTTGTTCATTTTTAAAAGTAGCATGTTGGTAATGTCGT250.14369467754914358No Hit
GTAGAAGTATCCTTGAAGGAATAGCTTTCGCTGCTTATGATGAAGGGATAA250.14369467754914358No Hit
ATTTTTTCTAAGAAAATACATAAAAACTTTAGGATTTCTTCTCTACATTCA250.14369467754914358No Hit
CTGGTTTTTGACTCTTTATGCAGCTCCTTCAGTAGTTTCATAGCAAGTGAG250.14369467754914358No Hit
GTTTGATGAAAGGTTCTTTGTCTTCTTCAGATGCTTTAACACCACTTTATT250.14369467754914358No Hit
GCTCTGTCAACATCATCTTGCAGGAGGTAAAGCAGACTCAGCTCTTGACTG240.13794689044717784No Hit
CGCAAGTTTCAGTCCTTGGTGTTTCTGGTTTGCTCGCTCCCTGAAGCTCTG240.13794689044717784No Hit
CTCCACACGACAAAGTACCACCTCCAGACAGAGTGTTTTCAACACTCCATG240.13794689044717784No Hit
CCTGAATACAAGAGACAAAGGGTGGAAAGAAAGAGAAGGTGGTATTAAGAA240.13794689044717784No Hit
CCGCGATCACCTTCTCTGAATCCTCTGAACTGGATCCAGAAAGCTCTAAGA240.13794689044717784No Hit
GTGGGAAACTGTCTATCTGCATCACCACGAGGTGCTCCAGAACTGGAGTAT230.1321991033452121No Hit
CGGGCACTGTCTATCATCTTCATTTTCATGGAAAACTTGCAACTCCTGATC230.1321991033452121No Hit
GTCAATATAAATTGACCATTCTTCTAGAGCATTCAGGCCTACTTCTGCCAA230.1321991033452121No Hit
GGGGTTTGGATAATCTGGGCTCATGCTGGTCATTTCGAGCAGAAAATTTGT230.1321991033452121No Hit
CTCAGATGGGTCTCTAGGATATCTTTGTATGGGGACATCTTTAGAGCTTTC230.1321991033452121No Hit
CTCCTTGTTGTTCGAATCCACATTTCTGATGATTCCATAAAACTGCTCCAT230.1321991033452121No Hit
GGCATCTGATAAACACGGTCGTCACCAGTGTCTGTCTGGGTGAGGAACATC230.1321991033452121No Hit
CGCGGAGTTCCTGGAGGAAATTCCCTGGACTGCATGGGGAAGTGAGCAACG230.1321991033452121No Hit
CTTATACATTAGCATGTAAGCTCCAACATCATGTTTTCCTGACATTTTTGT230.1321991033452121No Hit
GGCTGCATTACATGTCTGTCAATATAAATTGACCATTCTTCTAGAGCATTC230.1321991033452121No Hit
GGGCACACCACTTTAACAAGACTTCAAATAGAGACACGTAGTTGTCCAGAA230.1321991033452121No Hit
CCATGGTTCAAAAAATTCTGCTTGTTTCTCAGGGAGAATCTCTCTGCAAAA220.12645131624324635No Hit
CTGGCTTCTTTCCTAATTTCAATGTACTTTTTCTTTCTTTCCATAGGAACC220.12645131624324635No Hit
CTTTCATCATCTCTATGTCTCATGACTTGAACGAAGTCCTTGCTCTTTAAC220.12645131624324635No Hit
CTTGAAGTCTGGGGATCTTCTTCCATGGACTTAGTGAAGTTGCACAGAAGT220.12645131624324635No Hit
GCTGGATGCAAGTTAGCCGCTGGATCTGAAGTTGGGATCATCCAAACACCA220.12645131624324635No Hit
GTCGACATTCTGTCTGGGGCCTCCCACAATGAGCTAAAAGCCACTTGACCA220.12645131624324635No Hit
GTGGGCACACCACTTTAACAAGACTTCAAATAGAGACACGTAGTTGTCCAG220.12645131624324635No Hit
GGCTCTCTTACTGCTGATGTCATCTGGGTCTTAAGTTCACCCAGAAAAGCG220.12645131624324635No Hit
GTAGATATTATTAAAGGCAAGTGATGCTCCCAGCCTCTTGAAAGCATTGGG220.12645131624324635No Hit
CTCCATAGTATTCTGATGTTGCTTCAATTGTTTCGCAACCAGTTCACACAG210.12070352914128062No Hit
CTTGCCACAAACTCCTTATTCTTATGACCAGTAGAAGTATCCTTGAAGGAA210.12070352914128062No Hit
CTCACCTAATCTTTGATGTTACTATAACCTTATCTTTGATTTAACCCATAC210.12070352914128062No Hit
CTCGTTTTTGCTCAGCAACGCCTTTTCTGGCATACATCAAACTGAGCTTCT210.12070352914128062No Hit
CACGTAGTCTTTGTATGTGGGCACCTTCCATTTGCCAGTTCTGACTTCCCC210.12070352914128062No Hit
GGTTTGGATAATCTGGGCTCATGCTGGTCATTTCGAGCAGAAAATTTGTTG210.12070352914128062No Hit
CCTACATGTTGTAGAAGGATGGGAAACGAATTCCACAACGGGGTTCAGAAG210.12070352914128062No Hit
GTAGTCTTTGTATGTGGGCACCTTCCATTTGCCAGTTCTGACTTCCCCTGA210.12070352914128062No Hit
GTCAGAGCTCAGGAGATGTCTGAAGAGATCCACGTAGTCTTTGTATGTGGG200.11495574203931487No Hit
GGTCCATTTTAGCATCTGGATATCTGTTTGTCCAGGTGTTCAGAAGTCTCT200.11495574203931487No Hit
CCGGGCACTGTCTATCATCTTCATTTTCATGGAAAACTTGCAACTCCTGAT200.11495574203931487No Hit
CTCTCTTACTGCTGATGTCATCTGGGTCTTAAGTTCACCCAGAAAAGCGCG200.11495574203931487No Hit
GCTGCTTCTACAATGTTACATCAACTATACTTAGCTTTACTCTCCCAAAAT200.11495574203931487No Hit
CTCGAACATGGGGTTTGGATAATCTGGGCTCATGCTGGTCATTTCGAGCAG200.11495574203931487No Hit
CTGGAGAGTGCCCTGGGAGGCCTGGGTCTCCACAAACATCGGAGTGAGAAC200.11495574203931487No Hit
CTTTAGAATTAGGATCTTTACCGGAAAACTTTTCAAATATTAACCTATAAG200.11495574203931487No Hit
CTTGCTCTTTAACTGGAAGTACAGCTCTGTCATTCCCTCCACACGACAAAG200.11495574203931487No Hit
GCTGCATTACATGTCTGTCAATATAAATTGACCATTCTTCTAGAGCATTCA190.10920795493734912No Hit
GCCAGAACCAGAAGGGCATTCCAAGGCTTCCCCACATTTCCTCCATTTACG190.10920795493734912No Hit
CTTTAGTACAAAATTAAAAATCTCCCTTGAAGTCTGGGGATCTTCTTCCAT190.10920795493734912No Hit
CTCGAGGCAGGAACACATCCAGGAAAATGACAGGTTTCATCTCTCTAAAGG190.10920795493734912No Hit
GCCATTTTAGTTTCGAGAGGGGAATCTTTGGCCCACCATGAATCACACTTC190.10920795493734912No Hit
GTCCAGGGTTCTCATCGATCAATCCTTGAATCAGCACATCTTTTGCCAACT190.10920795493734912No Hit
CGGCGCTTCAGGTCGATCAGATTTTCAAAAATAAGCAAGTTCTTTTCTGGT190.10920795493734912No Hit
CCCATTTGCTGCTTCTCTGGCTTCTTTCCTAATTTCAATGTACTTTTTCTT190.10920795493734912No Hit
CTAACAACAACCATGGGATTTTTGAAGTTATCCCAAATTCAATGCAAAGCA190.10920795493734912No Hit
CTCCTCCATTGTTTTCAGAAGCAGCCAGCTGCAGCAAGGGGCTAAGCCAGT190.10920795493734912No Hit
CTGCATTATTTATCATCTCACTAGGATGAACTTCACCCAATAATCCTAGGA190.10920795493734912No Hit
GTTTTTGCTAGTTCAGCTCTTACATCATTGCTCCAATCCTTAAAGAGCAGT190.10920795493734912No Hit
TGTAGATATTATTAAAGGCAAGTGATGCTCCCAGCCTCTTGAAAGCATTGG190.10920795493734912No Hit
GTTTCTTTCATTGGTAACATCAGATTGATAAACTGGCGAGTTAGCCGAAAA190.10920795493734912No Hit
CTCCTAGAGATCCAAGCATTTGTACTACTCTAATTCTTATTTCTTCTAAGG190.10920795493734912No Hit
CTTGGCATCTGATAAACACGGTCGTCACCAGTGTCTGTCTGGGTGAGGAAC190.10920795493734912No Hit
GGGGTGATGAGGCTGCTGTGCTTGATCTGAATGTCAGGAAGGTCTCCGTGC180.10346016783538338No Hit
GTCAGAAGATTTTTGTTTATTTGTCCTCCTAGAGATCCAAGCATTTGTACT180.10346016783538338No Hit
TGGGTCTTCAGGAGAGTGTTTCAGACTCTTTGGACTAACTCCCTCGAAATA180.10346016783538338No Hit
TTCAGAAGTTCTCGGAGTTCTACTGGTTTTAACTTTGGCATCATCTTATAA180.10346016783538338No Hit
TGTCAATATAAATTGACCATTCTTCTAGAGCATTCAGGCCTACTTCTGCCA180.10346016783538338No Hit
CCCACATTTCCTCCATTTACGGAGACAGCTGTTTCACTAACTTGCAGCACT180.10346016783538338No Hit
ATTTTTTTTTTCAATGCAAGTTCTCCATAGAATTTACTAAATAATTCTCCA180.10346016783538338No Hit
CCGTTTCTTTCATTGGTAACATCAGATTGATAAACTGGCGAGTTAGCCGAA180.10346016783538338No Hit
CGGAGTTCTACTGGTTTTAACTTTGGCATCATCTTATAAATTATGTCCAAA180.10346016783538338No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)