FastQCFastQC Report
Сб 7 дек 2019
12_rna_chr8_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename12_rna_chr8_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17507
Sequences flagged as poor quality0
Sequence length39-51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCATTTTAGCATCTGGATATCTGTTTGTCCAGGTGTTCAGAAGTCTCTT1190.6797281087564974No Hit
GTTTGGATAATCTGGGCTCATGCTGGTCATTTCGAGCAGAAAATTTGTTGC730.41697606671617066No Hit
CCCTGATGCACCACAGTACTAATGCAATTCCTGAGAACTGGCCCTTTTGCT680.38841606214656993No Hit
CTGGGAGTCATTATCTGTCTCACTTTCTGGATCTCTGTAATTATCATGAAT620.35414405666304904No Hit
CCCGTTTCTTTCATTGGTAACATCAGATTGATAAACTGGCGAGTTAGCCGA610.34843205574912894No Hit
CTTCGTTTGATGAAAGGTTCTTTGTCTTCTTCAGATGCTTTAACACCACTT570.3255840520934483No Hit
GGGCTCTCTTACTGCTGATGTCATCTGGGTCTTAAGTTCACCCAGAAAAGC570.3255840520934483No Hit
GTCGCAAGTTTCAGTCCTTGGTGTTTCTGGTTTGCTCGCTCCCTGAAGCTC530.30273604843776775No Hit
CTGTAATGCCAGCACCGCGGGGCTGCTGCTCAGGACGCATTCCTGCCCCAG500.28560004569600733No Hit
CTTCAGGAGAGTGTTTCAGACTCTTTGGACTAACTCCCTCGAAATATTTTA480.274176043868167No Hit
GGGGTTTTTGCTAGTTCAGCTCTTACATCATTGCTCCAATCCTTAAAGAGC470.26846404295424686No Hit
GCCCATCTTCTTGGTGATTTGAGTATCAAAGGTAGATTCATTTAGCTTTGT460.2627520420403267No Hit
CTGGGTCTTCAGGAGAGTGTTTCAGACTCTTTGGACTAACTCCCTCGAAAT450.2570400411264066No Hit
CCCATCTTCTTGGTGATTTGAGTATCAAAGGTAGATTCATTTAGCTTTGTA440.25132804021248645No Hit
CTCGGAGTTCTACTGGTTTTAACTTTGGCATCATCTTATAAATTATGTCCA440.25132804021248645No Hit
CTCTGGGCCACGGCCTGTAACGGGGTGATGAGGCTGCTGTGCTTGATCTGA430.2456160392985663No Hit
CCCGCGATCACCTTCTCTGAATCCTCTGAACTGGATCCAGAAAGCTCTAAG420.23990403838464613No Hit
GGGGTATAGCTCAGGCCCAGTTTGAAAGCCATCTGCAGTGCAGGAACGTAG410.234192037470726No Hit
CTTTAAATGTAGATCCAGCTTTGTGTCAGCAAGTAGACTAATATATGTTGT390.2227680356428857No Hit
CTGGTATTTTTTTTTTCAATGCAAGTTCTCCATAGAATTTACTAAATAATT390.2227680356428857No Hit
CCATCTTCTTGGTGATTTGAGTATCAAAGGTAGATTCATTTAGCTTTGTAA370.2113440338150454No Hit
CTCCCTTGAAGTCTGGGGATCTTCTTCCATGGACTTAGTGAAGTTGCACAG360.20563203290112525No Hit
CTCAATTAATCCTAACCTGGAGGTCATGGGCACAACGCTATAGGTCCTCAG350.19992003198720512No Hit
GTCCCTCTGGGCCACGGCCTGTAACGGGGTGATGAGGCTGCTGTGCTTGAT340.19420803107328496No Hit
GCCCGTTTCTTTCATTGGTAACATCAGATTGATAAACTGGCGAGTTAGCCG340.19420803107328496No Hit
CTGATAAACACGGTCGTCACCAGTGTCTGTCTGGGTGAGGAACATCTGCTT340.19420803107328496No Hit
CTAGAAACTTTTTCATGCAGTCCACATAATTATTGAACCGCGGAGTTCCTG330.18849603015936484No Hit
CTTGGTGTTTCTGGTTTGCTCGCTCCCTGAAGCTCTGGTCTAACATGCCGT320.18278402924544468No Hit
CTGGCATCTGCGTGGCTTTGCCCAACATAAACATAACCATGCTATGTAAAA320.18278402924544468No Hit
GGGGGTATAGCTCAGGCCCAGTTTGAAAGCCATCTGCAGTGCAGGAACGTA320.18278402924544468No Hit
GTTCAGAAGTTCTCGGAGTTCTACTGGTTTTAACTTTGGCATCATCTTATA320.18278402924544468No Hit
CTCTCTCAGATGGGTCTCTAGGATATCTTTGTATGGGGACATCTTTAGAGC310.17707202833152452No Hit
CTCCTCTCCAGCAGCTGATTCTCTCCTGCCATGTTCTCTGTAAATGCATCG310.17707202833152452No Hit
CTCCGGTTGAGAAATCAAATTGACTCATTTCCTCACTCAGGGTACTGTCTG310.17707202833152452No Hit
CCGCGGAGTTCCTGGAGGAAATTCCCTGGACTGCATGGGGAAGTGAGCAAC310.17707202833152452No Hit
CTCTCAGATGGGTCTCTAGGATATCTTTGTATGGGGACATCTTTAGAGCTT300.1713600274176044No Hit
CATGGTTCAAAAAATTCTGCTTGTTTCTCAGGGAGAATCTCTCTGCAAAAT300.1713600274176044No Hit
GTGCTTTCCAATGTGCCAGAACCAGAAGGGCATTCCAAGGCTTCCCCACAT300.1713600274176044No Hit
CTTGTTTCTCAGGGAGAATCTCTCTGCAAAATTCCACCAGGTTAATGAAAG300.1713600274176044No Hit
CCTTGTTGTTCGAATCCACATTTCTGATGATTCCATAAAACTGCTCCATAA290.16564802650368424No Hit
GTTCCTATGCTGCTTCTACAATGTTACATCAACTATACTTAGCTTTACTCT290.16564802650368424No Hit
GTCCGCTTATAGAGCTGGTACATGGGTGGGGCTCCCTGTCCCCCTTCTGGC290.16564802650368424No Hit
GCCTTGTTTGCTTATAAAGCTGATGAACTCCTGAATTTCTGTTAAAGCCTG280.1599360255897641No Hit
CTGGCAATAAAGGAACGAATTTATAAAAGAGTTCAATGGATTTGTGTCGAC280.1599360255897641No Hit
CTCTGAACTCCGGTTGAGAAATCAAATTGACTCATTTCCTCACTCAGGGTA280.1599360255897641No Hit
GCCACCTCTTTGCCAAATTTCACAAATAAAGCAAAGCAAGAATACTTTTCT280.1599360255897641No Hit
GTGAGGAACATCTGCTTGCAGCGCTGAATGAGCTCAACGTACATGAAGTCA280.1599360255897641No Hit
CTCCTTCAGTAGTTTCATAGCAAGTGAGAAATTGTTCTGCTTCCGGGCACT280.1599360255897641No Hit
CTCGTTTTCTAAAAGACGTGACTGTTTCAGTACGATTAGCGCCCTTATACA280.1599360255897641No Hit
GTTCCCTACATGTTGTAGAAGGATGGGAAACGAATTCCACAACGGGGTTCA280.1599360255897641No Hit
GTCCTCCTAGAGATCCAAGCATTTGTACTACTCTAATTCTTATTTCTTCTA270.15422402467584395No Hit
GGGGTTCAGAAGTTCTCGGAGTTCTACTGGTTTTAACTTTGGCATCATCTT260.1485120237619238No Hit
CTTGTTGTTCGAATCCACATTTCTGATGATTCCATAAAACTGCTCCATAAA260.1485120237619238No Hit
GTCATAGGGAGGCAGGTCATTGGCCATCACGATGCCTAGCAATTGAATCCC260.1485120237619238No Hit
CTTTGAGATAATTCCAATGCATCTAGAAACTTTTTCATGCAGTCCACATAA260.1485120237619238No Hit
GGGGCTTTGAGTCTTTGTTCATTTTTAAAAGTAGCATGTTGGTAATGTCGT250.14280002284800367No Hit
GTAGAAGTATCCTTGAAGGAATAGCTTTCGCTGCTTATGATGAAGGGATAA250.14280002284800367No Hit
ATTTTTTCTAAGAAAATACATAAAAACTTTAGGATTTCTTCTCTACATTCA250.14280002284800367No Hit
CTGGTTTTTGACTCTTTATGCAGCTCCTTCAGTAGTTTCATAGCAAGTGAG250.14280002284800367No Hit
GTTTGATGAAAGGTTCTTTGTCTTCTTCAGATGCTTTAACACCACTTTATT250.14280002284800367No Hit
GCTCTGTCAACATCATCTTGCAGGAGGTAAAGCAGACTCAGCTCTTGACTG240.1370880219340835No Hit
CGCAAGTTTCAGTCCTTGGTGTTTCTGGTTTGCTCGCTCCCTGAAGCTCTG240.1370880219340835No Hit
CTCCACACGACAAAGTACCACCTCCAGACAGAGTGTTTTCAACACTCCATG240.1370880219340835No Hit
CCTGAATACAAGAGACAAAGGGTGGAAAGAAAGAGAAGGTGGTATTAAGAA240.1370880219340835No Hit
CCGCGATCACCTTCTCTGAATCCTCTGAACTGGATCCAGAAAGCTCTAAGA240.1370880219340835No Hit
GTGGGAAACTGTCTATCTGCATCACCACGAGGTGCTCCAGAACTGGAGTAT230.13137602102016335No Hit
CGGGCACTGTCTATCATCTTCATTTTCATGGAAAACTTGCAACTCCTGATC230.13137602102016335No Hit
GTCAATATAAATTGACCATTCTTCTAGAGCATTCAGGCCTACTTCTGCCAA230.13137602102016335No Hit
GGGGTTTGGATAATCTGGGCTCATGCTGGTCATTTCGAGCAGAAAATTTGT230.13137602102016335No Hit
CTCAGATGGGTCTCTAGGATATCTTTGTATGGGGACATCTTTAGAGCTTTC230.13137602102016335No Hit
CTCCTTGTTGTTCGAATCCACATTTCTGATGATTCCATAAAACTGCTCCAT230.13137602102016335No Hit
GGCATCTGATAAACACGGTCGTCACCAGTGTCTGTCTGGGTGAGGAACATC230.13137602102016335No Hit
CGCGGAGTTCCTGGAGGAAATTCCCTGGACTGCATGGGGAAGTGAGCAACG230.13137602102016335No Hit
CTTATACATTAGCATGTAAGCTCCAACATCATGTTTTCCTGACATTTTTGT230.13137602102016335No Hit
GGCTGCATTACATGTCTGTCAATATAAATTGACCATTCTTCTAGAGCATTC230.13137602102016335No Hit
GGGCACACCACTTTAACAAGACTTCAAATAGAGACACGTAGTTGTCCAGAA230.13137602102016335No Hit
CCATGGTTCAAAAAATTCTGCTTGTTTCTCAGGGAGAATCTCTCTGCAAAA220.12566402010624322No Hit
CTGGCTTCTTTCCTAATTTCAATGTACTTTTTCTTTCTTTCCATAGGAACC220.12566402010624322No Hit
CTTTCATCATCTCTATGTCTCATGACTTGAACGAAGTCCTTGCTCTTTAAC220.12566402010624322No Hit
CTTGAAGTCTGGGGATCTTCTTCCATGGACTTAGTGAAGTTGCACAGAAGT220.12566402010624322No Hit
GCTGGATGCAAGTTAGCCGCTGGATCTGAAGTTGGGATCATCCAAACACCA220.12566402010624322No Hit
GTCGACATTCTGTCTGGGGCCTCCCACAATGAGCTAAAAGCCACTTGACCA220.12566402010624322No Hit
GTGGGCACACCACTTTAACAAGACTTCAAATAGAGACACGTAGTTGTCCAG220.12566402010624322No Hit
GGCTCTCTTACTGCTGATGTCATCTGGGTCTTAAGTTCACCCAGAAAAGCG220.12566402010624322No Hit
GTAGATATTATTAAAGGCAAGTGATGCTCCCAGCCTCTTGAAAGCATTGGG220.12566402010624322No Hit
CTCCATAGTATTCTGATGTTGCTTCAATTGTTTCGCAACCAGTTCACACAG210.11995201919232307No Hit
CTTGCCACAAACTCCTTATTCTTATGACCAGTAGAAGTATCCTTGAAGGAA210.11995201919232307No Hit
CTCACCTAATCTTTGATGTTACTATAACCTTATCTTTGATTTAACCCATAC210.11995201919232307No Hit
CTCGTTTTTGCTCAGCAACGCCTTTTCTGGCATACATCAAACTGAGCTTCT210.11995201919232307No Hit
CACGTAGTCTTTGTATGTGGGCACCTTCCATTTGCCAGTTCTGACTTCCCC210.11995201919232307No Hit
GGTTTGGATAATCTGGGCTCATGCTGGTCATTTCGAGCAGAAAATTTGTTG210.11995201919232307No Hit
CCTACATGTTGTAGAAGGATGGGAAACGAATTCCACAACGGGGTTCAGAAG210.11995201919232307No Hit
GTAGTCTTTGTATGTGGGCACCTTCCATTTGCCAGTTCTGACTTCCCCTGA210.11995201919232307No Hit
GTCAGAGCTCAGGAGATGTCTGAAGAGATCCACGTAGTCTTTGTATGTGGG200.11424001827840292No Hit
GGTCCATTTTAGCATCTGGATATCTGTTTGTCCAGGTGTTCAGAAGTCTCT200.11424001827840292No Hit
CCGGGCACTGTCTATCATCTTCATTTTCATGGAAAACTTGCAACTCCTGAT200.11424001827840292No Hit
CTCTCTTACTGCTGATGTCATCTGGGTCTTAAGTTCACCCAGAAAAGCGCG200.11424001827840292No Hit
GCTGCTTCTACAATGTTACATCAACTATACTTAGCTTTACTCTCCCAAAAT200.11424001827840292No Hit
CTCGAACATGGGGTTTGGATAATCTGGGCTCATGCTGGTCATTTCGAGCAG200.11424001827840292No Hit
CTGGAGAGTGCCCTGGGAGGCCTGGGTCTCCACAAACATCGGAGTGAGAAC200.11424001827840292No Hit
CTTTAGAATTAGGATCTTTACCGGAAAACTTTTCAAATATTAACCTATAAG200.11424001827840292No Hit
CTTGCTCTTTAACTGGAAGTACAGCTCTGTCATTCCCTCCACACGACAAAG200.11424001827840292No Hit
GCTGCATTACATGTCTGTCAATATAAATTGACCATTCTTCTAGAGCATTCA190.10852801736448278No Hit
GCCAGAACCAGAAGGGCATTCCAAGGCTTCCCCACATTTCCTCCATTTACG190.10852801736448278No Hit
CTTTAGTACAAAATTAAAAATCTCCCTTGAAGTCTGGGGATCTTCTTCCAT190.10852801736448278No Hit
CTCGAGGCAGGAACACATCCAGGAAAATGACAGGTTTCATCTCTCTAAAGG190.10852801736448278No Hit
GCCATTTTAGTTTCGAGAGGGGAATCTTTGGCCCACCATGAATCACACTTC190.10852801736448278No Hit
GTCCAGGGTTCTCATCGATCAATCCTTGAATCAGCACATCTTTTGCCAACT190.10852801736448278No Hit
CGGCGCTTCAGGTCGATCAGATTTTCAAAAATAAGCAAGTTCTTTTCTGGT190.10852801736448278No Hit
CCCATTTGCTGCTTCTCTGGCTTCTTTCCTAATTTCAATGTACTTTTTCTT190.10852801736448278No Hit
CTAACAACAACCATGGGATTTTTGAAGTTATCCCAAATTCAATGCAAAGCA190.10852801736448278No Hit
CTCCTCCATTGTTTTCAGAAGCAGCCAGCTGCAGCAAGGGGCTAAGCCAGT190.10852801736448278No Hit
CTGCATTATTTATCATCTCACTAGGATGAACTTCACCCAATAATCCTAGGA190.10852801736448278No Hit
GTTTTTGCTAGTTCAGCTCTTACATCATTGCTCCAATCCTTAAAGAGCAGT190.10852801736448278No Hit
TGTAGATATTATTAAAGGCAAGTGATGCTCCCAGCCTCTTGAAAGCATTGG190.10852801736448278No Hit
GTTTCTTTCATTGGTAACATCAGATTGATAAACTGGCGAGTTAGCCGAAAA190.10852801736448278No Hit
CTCCTAGAGATCCAAGCATTTGTACTACTCTAATTCTTATTTCTTCTAAGG190.10852801736448278No Hit
CTTGGCATCTGATAAACACGGTCGTCACCAGTGTCTGTCTGGGTGAGGAAC190.10852801736448278No Hit
GGGGTGATGAGGCTGCTGTGCTTGATCTGAATGTCAGGAAGGTCTCCGTGC180.10281601645056263No Hit
GTCAGAAGATTTTTGTTTATTTGTCCTCCTAGAGATCCAAGCATTTGTACT180.10281601645056263No Hit
TGGGTCTTCAGGAGAGTGTTTCAGACTCTTTGGACTAACTCCCTCGAAATA180.10281601645056263No Hit
TTCAGAAGTTCTCGGAGTTCTACTGGTTTTAACTTTGGCATCATCTTATAA180.10281601645056263No Hit
TGTCAATATAAATTGACCATTCTTCTAGAGCATTCAGGCCTACTTCTGCCA180.10281601645056263No Hit
CCCACATTTCCTCCATTTACGGAGACAGCTGTTTCACTAACTTGCAGCACT180.10281601645056263No Hit
ATTTTTTTTTTCAATGCAAGTTCTCCATAGAATTTACTAAATAATTCTCCA180.10281601645056263No Hit
CCGTTTCTTTCATTGGTAACATCAGATTGATAAACTGGCGAGTTAGCCGAA180.10281601645056263No Hit
CGGAGTTCTACTGGTTTTAACTTTGGCATCATCTTATAAATTATGTCCAAA180.10281601645056263No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)