Task 1
For this task I've chosen 6 proteins from my previous work (pr10) . I've also decided to perform multiple alignment in 4 different services and compare results of any 2 of them.
Table 1. Main information about chosen proteins
Entry name Length Protein name Organism Phylum DNAK_THEVO
613
Chaperone protein DnaK
Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)
Archaea
DNAK_LACAC
614
Chaperone protein DnaK
Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)
Bacteria
DNAK_SALTY
638
Chaperone protein DnaK
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Bacteria
BIP1_ORYSJ
665
Heat shock 70 kDa protein BIP1
Oryza sativa subsp. japonica (Rice)
Eukaryota
DNAK_HALWD
641
Chaperone protein DnaK
Haloquadratum walsbyi (strain DSM 16790 / HBSQ001)
Archaea
HSP74_DROME
641
Major heat shock 70 kDa protein Bbb
Drosophila melanogaster (Fruit fly)
Eukaryota
Table 2. Description of used programs
Name Description[1]
TCoffee
"Consistency-based MSA tool that attempts to mitigate the pitfalls of progressive alignment methods. Suitable for small alignments."
Muscle
"Accurate MSA tool, especially good with proteins. Suitable for medium alignments."
Mafft
"MSA tool that uses Fast Fourier Transforms. Suitable for medium-large alignments."
PRANK (WebPRANK)
"The new phylogeny-aware multiple sequence alignment program which makes use of evolutionary information to help place insertions and deletions."
Alignments
{"seqs":[{"name":"UNIPROT|Q97BG8|DNAK_THEVO/1-613","start":1,"end":613,"id":"615680295","seq":"MS-------------------------------KIIGIDLGTSNSAAAVVISGKPTVIPSSEGVSIGGKAFPSYVAFTKDGQMLVGEPARRQALLNPEGTIFAAKRKMGTDYK---------------------------FKV-FDKEFTPQQISAFILQKIKKDAEAFLGEPVNEAVITVPAYFNDNQRQATKDAGTIAGFDVKRIINEPTAAALAYGVDKSG-KSEKILVFDLGGGTLDVTIIEISK----RPNVQVLSTSGDTQLGGTDMDEAIVNYIADDFQKKEGIDLRKDRGAYIRLRDAAEKAKIELSTTLSSDIDLPYITVTSSGPKHIKMTLTRAKLEELISPIVERVKAPIDKALEGAKLKKTDITKLLFVGGPTRIPYVRKYVEDYL-GIKAEGGVDPMEAVAIGAAIQGAVLKGE----IKDIVLLDVTPLTLSVETLGGIATPIIPANTTIPVRKSQVFTTAEDMQTTVTIHVVQGERPLAKDNVSLGMFNLTGIAPAPRGIPQIEVTFDIDSNGILNVTAVDKATGKKQGITITASTK-LSKDEIERMKKEAEQYAEQDRKMKEQIETLNNAESLAYSVEKTLNEA---GDKVDKETKDRILSEVKDLRKAIEEKN---MDNVKTLMEKISKDIQEVGTKMYQSASSTTQTGSGNQNSSKQENDKT-----------------------VDAEYK------EKS","order":1},{"name":"UNIPROT|Q84BU4|Q5FJP4|DNAK_LACAC/1-614","start":1,"end":614,"id":"774686013","seq":"MS-------------------------------KVIGIDLGTTNSAVAVLEGKEPKIITNPEG----NRTTPSVVAFK-DGEIQVGEVAKRQAITNP-NTIVSIKRHMGEA--------------------------DYKVKV-GDKSYTPQEISAFILQYIKKFSEDYLGEEVKDAVITVPAYFNDAQRQATKDAGKIAGLNVQRIINEPTASALAYGLDKDD-DDEKVLVYDLGGGTFDVSVLQLGD----G-VFQVLSTNGDTHLGGDDFDNRIMDWLIKNFKDENGVDLSKDKMAMQRLKDASEKAKKDLSGVSSTHISLPFISAGESGPLHLEADLTRAKFDELTNDLVEKTKIPFDNALKDAGLTVNDIDKVILNGGSTRIPAVQKAVKEWA-GKEPDHSINPDEAVALGAAIQGGVISGD----VKDIVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSKSQIFSTAADNQPAVDVHVLQGERPMAADDKTLGRFELTDIPPAPRGVPQIQVTFDIDKNGIVNVSAKDMGTGKEQKITIKSSSG-LSDEEIKRMQKDAEEHAEEDKKRKDEADLRNEVDQLIFTTEKTLKET---KGKVSDEDTKKVQEALDDLKKAQKDNN---LDEMKEKKDALSKAAQDLAVKLYQQNGGAQGAA-G------QAGP------------QGGNPNDGNNGGAQDGEFHKVD---PNK","order":2},{"name":"UNIPROT|Q56073|DNAK_SALTY/1-638","start":1,"end":638,"id":"60077389","seq":"MG-------------------------------KIIGIDLGTTNSCVAIMDGTQARVLENAEG----DRTTPSIIAYTQDGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPYKIIGADNG---DAWLDV-KGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAAALAYGLDKEV-GNRTIAVYDLGGGTFDISIIEIDEVDGEK-TFEVLATNGDTHLGGEDFDTRLINYLVDEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDINDVILVGGQTRMPMVQKKVAEFF-GKEPRKDVNPDEAVAIGAAVQGGVLTGD----VKDVLLLDVTPLSLGIETMGGVMTPLITKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRASDNKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSG-LNEEEIQKMVRDAEANAESDRKFEELVQTRNQGDHLLHSTRKQVEEA---GDKLPADDKTAIESALNALETALKGED---KAAIEAKMQELAQVSQKLMEIAQQQHAQQQ------------AGS------------ADASANNAKDDDVVDAEFEEVK---DKK","order":3},{"name":"UNIPROT|P11147|Q3KN45|Q8SXQ4|Q9VFB0|HSP7D_DROME/1-651","start":1,"end":651,"id":"729978399","seq":"MSKA-----------------------------PAVGIDLGTTYSCVGVFQHGKVEIIANDQG----NRTTPSYVAFT-DTERLIGDAAKNQVAMNPTQTIFDAKRLIGRKFDDAAVQSDMKHWPFEVVSADGKPKIEVTYKD-EKKTFFPEEISSMVLTKMKETAEAYLGKTVTNAVITVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKAVGERNVLIFDLGGGTFDVSILSIDD----G-IFEVKSTAGDTHLGGEDFDNRLVTHFVQEFKRKHKKDLTTNKRALRRLRTACERAKRTLSSSTQASIEIDSLFEG----TDFYTSITRARFEELNADLFRSTMDPVEKALRDAKLDKSVIHDIVLVGGSTRIPKVQRLLQDLFNGKELNKSINPDEAVAYGAAVQAAILHGDKSQEVQDLLLLDVTPLSLGIETAGGVMSVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQIEVTFDIDANGILNVTALERSTNKENKITITNDKGRLSKEDIERMVNEAEKYRNEDEKQKETIAAKNGLESYCFNMKATLDEDN-LKTKISDSDRTTILDKCNETIKWLDANQLADKEEYEHRQKELEGVCNPIITKLYQGAGFPPGGM--------PGGPGGMP---GAAGAA-----GAAGAGGAGPTIE------EVD","order":4},{"name":"UNIPROT|Q6Z7B0|O24182|BIP1_ORYSJ/1-665","start":1,"end":665,"id":"466209273","seq":"MDRVRGCAFLLGVLLAGSLFAFSVAKEETKKLGTVIGIDLGTTYSCVGVYKNGHVEIIANDQG----NRITPSWVAFT-DSERLIGEAAKNQAAVNPERTIFDVKRLIGRKFEDKEVQRDMKLVPYKIVNKDGKPYIQVKIKDGENKVFSPEEVSAMILGKMKETAEAYLGKKINDAVVTVPAYFNDAQRQATKDAGVIAGLNVARIINEPTAAAIAYGLDKKG-GEKNILVFDLGGGTFDVSILTIDN----G-VFEVLATNGDTHLGGEDFDQRIMEYFIKLIKKKYSKDISKDNRALGKLRREAERAKRALSNQHQVRVEIESLFDG----TDFSEPLTRARFEELNNDLFRKTMGPVKKAMDDAGLEKSQIHEIVLVGGSTRIPKVQQLLRDYFEGKEPNKGVNPDEAVAYGAAVQGSILSGEGGDETKDILLLDVAPLTLGIETVGGVMTKLIPRNTVIPTKKSQVFTTYQDQQTTVSIQVFEGERSMTKDCRLLGKFDLSGIPAAPRGTPQIEVTFEVDANGILNVKAEDKGTGKSEKITITNEKGRLSQEEIDRMVREAEEFAEEDKKVKERIDARNQLETYVYNMKNTVGDKDKLADKLESEEKEKVEEALKEALEWLDENQTAEKEEYEEKLKEVEAVCNPIISAVYQRTGGAPGGG--------ADGEGG-----------------------VDDEH-------DEL","order":5},{"name":"UNIPROT|Q18GZ4|DNAK_HALWD/1-641","start":1,"end":641,"id":"1392829072","seq":"MASN-----------------------------KILGIDLGTTNSAFAVMEGDDPEIIVNAEG----DRTTPSVVAMTDDEERLVGKPAKNQVIQNPDQTIRSIKRHMGEE--------------------------DYTVEL-GGEDYTPEQVSAMILQKIKRDAEEYLGDEIEKAVITVPAYFNDRQRQATKDAGEIAGFEVDRIVNEPTAASMAYGLDDES--NQTILVYDLGGGTFDVSVLDLGG----G-VYEVVATNGDNDLGGDDWDDAVIDWLAGEFEDNHGIDLRDDRQALQRLKDAAEEAKIELSSRKETTINLPFITATDSGPVHLEETLSRAKFESLTEDLIERTVGPTEQALEDAGYDDSDIDEVILVGGSTRMPQVREKVEDLL-GTEPKKNVNPDEAVALGAAIQGGVLAGD----VDDIVLLDVTPLSLGIEVKGGLFERLIDKNTTIPTEESKVFTTAAANQTSVNVRVFQGEREIAEENELLGEFQLAGIPPAPAGTPQIEVTFNIDENGIVNVEAEDQGSGNAESITIEGGAG-LSDEEIEQMQEEAEAHAEEDERRRERIEARNSAESAVQRAETLLEEN---EEDIDDDLKESIEDEVESVEAVLEDED-ATKEEIEDVTESLSSELQEIGKQMYDAQQAAAGAGAGAAGAGAGAGPGGMGDMGDMGDMGGAAGSGDADNEYVDADFEDVDDDTKDE","order":6}],"appSettings":{"globalColorScheme":"Clustal","webStartUrl":"http://www.jalview.org/services/launchApp","application":"Jalview","showSeqFeatures":"false","version":"2.10.1"},"seqGroups":[],"alignAnnotation":[],"svid":"1.0","seqFeatures":[]}
UNIPROT|Q97BG8|DNAK_THEVO/1-613 UNIPROT|Q84BU4|Q5FJP4|DNAK_LACAC/1-614 UNIPROT|Q56073|DNAK_SALTY/1-638 UNIPROT|P11147|Q3KN45|Q8SXQ4|Q9VFB0|HSP7D_DROME/1-651 UNIPROT|Q6Z7B0|O24182|BIP1_ORYSJ/1-665 UNIPROT|Q18GZ4|DNAK_HALWD/1-641 Conservation Quality Consensus
10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 710 M S - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - K I I G I D L G T S N S A A A V V I S G K P T V I P S S E G V S I G G K A F P S Y V A F T K D G Q M L V G E P A R R Q A L L N P E G T I F A A K R K M G T D Y K - - - - - - - - - - - - - - - - - - - - - - - - - - - F K V - F D K E F T P Q Q I S A F I L Q K I K K D A E A F L G E P V N E A V I T V P A Y F N D N Q R Q A T K D A G T I A G F D V K R I I N E P T A A A L A Y G V D K S G - K S E K I L V F D L G G G T L D V T I I E I S K - - - - R P N V Q V L S T S G D T Q L G G T D M D E A I V N Y I A D D F Q K K E G I D L R K D R G A Y I R L R D A A E K A K I E L S T T L S S D I D L P Y I T V T S S G P K H I K M T L T R A K L E E L I S P I V E R V K A P I D K A L E G A K L K K T D I T K L L F V G G P T R I P Y V R K Y V E D Y L - G I K A E G G V D P M E A V A I G A A I Q G A V L K G E - - - - I K D I V L L D V T P L T L S V E T L G G I A T P I I P A N T T I P V R K S Q V F T T A E D M Q T T V T I H V V Q G E R P L A K D N V S L G M F N L T G I A P A P R G I P Q I E V T F D I D S N G I L N V T A V D K A T G K K Q G I T I T A S T K - L S K D E I E R M K K E A E Q Y A E Q D R K M K E Q I E T L N N A E S L A Y S V E K T L N E A - - - G D K V D K E T K D R I L S E V K D L R K A I E E K N - - - M D N V K T L M E K I S K D I Q E V G T K M Y Q S A S S T T Q T G S G N Q N S S K Q E N D K T - - - - - - - - - - - - - - - - - - - - - - - V D A E Y K - - - - - - E K S M S - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - K V I G I D L G T T N S A V A V L E G K E P K I I T N P E G - - - - N R T T P S V V A F K - D G E I Q V G E V A K R Q A I T N P - N T I V S I K R H M G E A - - - - - - - - - - - - - - - - - - - - - - - - - - D Y K V K V - G D K S Y T P Q E I S A F I L Q Y I K K F S E D Y L G E E V K D A V I T V P A Y F N D A Q R Q A T K D A G K I A G L N V Q R I I N E P T A S A L A Y G L D K D D - D D E K V L V Y D L G G G T F D V S V L Q L G D - - - - G - V F Q V L S T N G D T H L G G D D F D N R I M D W L I K N F K D E N G V D L S K D K M A M Q R L K D A S E K A K K D L S G V S S T H I S L P F I S A G E S G P L H L E A D L T R A K F D E L T N D L V E K T K I P F D N A L K D A G L T V N D I D K V I L N G G S T R I P A V Q K A V K E W A - G K E P D H S I N P D E A V A L G A A I Q G G V I S G D - - - - V K D I V L L D V T P L S L G I E T M G G V F T K L I D R N T T I P T S K S Q I F S T A A D N Q P A V D V H V L Q G E R P M A A D D K T L G R F E L T D I P P A P R G V P Q I Q V T F D I D K N G I V N V S A K D M G T G K E Q K I T I K S S S G - L S D E E I K R M Q K D A E E H A E E D K K R K D E A D L R N E V D Q L I F T T E K T L K E T - - - K G K V S D E D T K K V Q E A L D D L K K A Q K D N N - - - L D E M K E K K D A L S K A A Q D L A V K L Y Q Q N G G A Q G A A - G - - - - - - Q A G P - - - - - - - - - - - - Q G G N P N D G N N G G A Q D G E F H K V D - - - P N K M G - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - K I I G I D L G T T N S C V A I M D G T Q A R V L E N A E G - - - - D R T T P S I I A Y T Q D G E T L V G Q P A K R Q A V T N P Q N T L F A I K R L I G R R F Q D E E V Q R D V S I M P Y K I I G A D N G - - - D A W L D V - K G Q K M A P P Q I S A E V L K K M K K T A E D Y L G E P V T E A V I T V P A Y F N D A Q R Q A T K D A G R I A G L E V K R I I N E P T A A A L A Y G L D K E V - G N R T I A V Y D L G G G T F D I S I I E I D E V D G E K - T F E V L A T N G D T H L G G E D F D T R L I N Y L V D E F K K D Q G I D L R N D P L A M Q R L K E A A E K A K I E L S S A Q Q T D V N L P Y I T A D A T G P K H M N I K V T R A K L E S L V E D L V N R S I E P L K V A L Q D A G L S V S D I N D V I L V G G Q T R M P M V Q K K V A E F F - G K E P R K D V N P D E A V A I G A A V Q G G V L T G D - - - - V K D V L L L D V T P L S L G I E T M G G V M T P L I T K N T T I P T K H S Q V F S T A E D N Q S A V T I H V L Q G E R K R A S D N K S L G Q F N L D G I N P A P R G M P Q I E V T F D I D A D G I L H V S A K D K N S G K E Q K I T I K A S S G - L N E E E I Q K M V R D A E A N A E S D R K F E E L V Q T R N Q G D H L L H S T R K Q V E E A - - - G D K L P A D D K T A I E S A L N A L E T A L K G E D - - - K A A I E A K M Q E L A Q V S Q K L M E I A Q Q Q H A Q Q Q - - - - - - - - - - - - A G S - - - - - - - - - - - - A D A S A N N A K D D D V V D A E F E E V K - - - D K K M S K A - - - - - - - - - - - - - - - - - - - - - - - - - - - - - P A V G I D L G T T Y S C V G V F Q H G K V E I I A N D Q G - - - - N R T T P S Y V A F T - D T E R L I G D A A K N Q V A M N P T Q T I F D A K R L I G R K F D D A A V Q S D M K H W P F E V V S A D G K P K I E V T Y K D - E K K T F F P E E I S S M V L T K M K E T A E A Y L G K T V T N A V I T V P A Y F N D S Q R Q A T K D A G T I A G L N V L R I I N E P T A A A I A Y G L D K K A V G E R N V L I F D L G G G T F D V S I L S I D D - - - - G - I F E V K S T A G D T H L G G E D F D N R L V T H F V Q E F K R K H K K D L T T N K R A L R R L R T A C E R A K R T L S S S T Q A S I E I D S L F E G - - - - T D F Y T S I T R A R F E E L N A D L F R S T M D P V E K A L R D A K L D K S V I H D I V L V G G S T R I P K V Q R L L Q D L F N G K E L N K S I N P D E A V A Y G A A V Q A A I L H G D K S Q E V Q D L L L L D V T P L S L G I E T A G G V M S V L I K R N T T I P T K Q T Q T F T T Y S D N Q P G V L I Q V Y E G E R A M T K D N N L L G K F E L S G I P P A P R G V P Q I E V T F D I D A N G I L N V T A L E R S T N K E N K I T I T N D K G R L S K E D I E R M V N E A E K Y R N E D E K Q K E T I A A K N G L E S Y C F N M K A T L D E D N - L K T K I S D S D R T T I L D K C N E T I K W L D A N Q L A D K E E Y E H R Q K E L E G V C N P I I T K L Y Q G A G F P P G G M - - - - - - - - P G G P G G M P - - - G A A G A A - - - - - G A A G A G G A G P T I E - - - - - - E V D M D R V R G C A F L L G V L L A G S L F A F S V A K E E T K K L G T V I G I D L G T T Y S C V G V Y K N G H V E I I A N D Q G - - - - N R I T P S W V A F T - D S E R L I G E A A K N Q A A V N P E R T I F D V K R L I G R K F E D K E V Q R D M K L V P Y K I V N K D G K P Y I Q V K I K D G E N K V F S P E E V S A M I L G K M K E T A E A Y L G K K I N D A V V T V P A Y F N D A Q R Q A T K D A G V I A G L N V A R I I N E P T A A A I A Y G L D K K G - G E K N I L V F D L G G G T F D V S I L T I D N - - - - G - V F E V L A T N G D T H L G G E D F D Q R I M E Y F I K L I K K K Y S K D I S K D N R A L G K L R R E A E R A K R A L S N Q H Q V R V E I E S L F D G - - - - T D F S E P L T R A R F E E L N N D L F R K T M G P V K K A M D D A G L E K S Q I H E I V L V G G S T R I P K V Q Q L L R D Y F E G K E P N K G V N P D E A V A Y G A A V Q G S I L S G E G G D E T K D I L L L D V A P L T L G I E T V G G V M T K L I P R N T V I P T K K S Q V F T T Y Q D Q Q T T V S I Q V F E G E R S M T K D C R L L G K F D L S G I P A A P R G T P Q I E V T F E V D A N G I L N V K A E D K G T G K S E K I T I T N E K G R L S Q E E I D R M V R E A E E F A E E D K K V K E R I D A R N Q L E T Y V Y N M K N T V G D K D K L A D K L E S E E K E K V E E A L K E A L E W L D E N Q T A E K E E Y E E K L K E V E A V C N P I I S A V Y Q R T G G A P G G G - - - - - - - - A D G E G G - - - - - - - - - - - - - - - - - - - - - - - V D D E H - - - - - - - D E L M A S N - - - - - - - - - - - - - - - - - - - - - - - - - - - - - K I L G I D L G T T N S A F A V M E G D D P E I I V N A E G - - - - D R T T P S V V A M T D D E E R L V G K P A K N Q V I Q N P D Q T I R S I K R H M G E E - - - - - - - - - - - - - - - - - - - - - - - - - - D Y T V E L - G G E D Y T P E Q V S A M I L Q K I K R D A E E Y L G D E I E K A V I T V P A Y F N D R Q R Q A T K D A G E I A G F E V D R I V N E P T A A S M A Y G L D D E S - - N Q T I L V Y D L G G G T F D V S V L D L G G - - - - G - V Y E V V A T N G D N D L G G D D W D D A V I D W L A G E F E D N H G I D L R D D R Q A L Q R L K D A A E E A K I E L S S R K E T T I N L P F I T A T D S G P V H L E E T L S R A K F E S L T E D L I E R T V G P T E Q A L E D A G Y D D S D I D E V I L V G G S T R M P Q V R E K V E D L L - G T E P K K N V N P D E A V A L G A A I Q G G V L A G D - - - - V D D I V L L D V T P L S L G I E V K G G L F E R L I D K N T T I P T E E S K V F T T A A A N Q T S V N V R V F Q G E R E I A E E N E L L G E F Q L A G I P P A P A G T P Q I E V T F N I D E N G I V N V E A E D Q G S G N A E S I T I E G G A G - L S D E E I E Q M Q E E A E A H A E E D E R R R E R I E A R N S A E S A V Q R A E T L L E E N - - - E E D I D D D L K E S I E D E V E S V E A V L E D E D - A T K E E I E D V T E S L S S E L Q E I G K Q M Y D A Q Q A A A G A G A G A A G A G A G A G P G G M G D M G D M G D M G G A A G S G D A D N E Y V D A D F E D V D D D T K D E * 5 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 4 7 9 * * * * * * 8 7 * 9 6 + 9 6 3 3 3 4 6 5 9 + 2 9 4 8 * - - - - 5 9 6 7 * * 6 9 * 8 7 - * 4 8 4 7 9 * 5 6 * 9 7 * 8 7 6 * * 1 4 * + 3 5 7 * * 5 9 * 5 3 - - - - - - - - - - - - - - - - - - - - - - - - - - 1 1 2 6 6 4 - 2 3 6 2 8 5 * 5 8 9 * 8 5 9 * 4 7 9 * 7 4 8 * 4 9 * * 6 3 9 5 5 * * 9 * * * * * * * * 4 * * * * * * * * * 3 * * * 8 7 * 2 * * 9 * * * * * 8 8 9 * * * 9 * 6 4 4 - 0 6 6 6 9 7 9 9 * * * * * * 8 * 9 8 9 + 5 + 5 3 - - - - 4 - 6 6 8 * 5 8 * 7 * * 9 4 * * * 6 * 8 * 6 4 9 8 6 8 8 7 3 4 8 6 6 5 4 4 5 * + 5 5 8 4 3 * 7 3 9 * 9 5 4 7 * 7 * * 5 4 * * 7 3 2 6 6 3 9 6 + 5 5 + 5 3 5 - - - - 5 5 8 3 3 2 9 8 * * 9 8 9 6 * 6 4 6 + 7 6 5 6 5 3 * 6 6 4 * 9 5 5 * 5 7 4 3 8 4 * 4 6 9 9 8 7 * * 6 * * 9 * 3 * 8 6 3 9 3 9 7 6 - * 5 7 5 5 4 5 9 8 * 5 * * * * 7 * * * 9 * + 8 9 + 3 * 9 - - - - 7 5 * 9 9 * * * * 8 * * 8 * 8 9 * 8 4 * * 9 7 5 3 + * 3 4 * * 8 * * 8 4 5 8 7 7 * 8 * 6 4 5 7 * 6 7 * 3 9 6 * 6 8 * * * 2 5 8 3 9 6 3 5 * * 5 * 7 * 5 5 * 6 7 * * 4 * 7 * * * 8 * * * 7 9 * 3 8 * * 9 5 * 4 * 3 9 6 7 8 7 6 3 7 4 * * * 5 7 4 4 5 - * 9 5 9 9 * 5 7 * 4 5 9 * * 3 4 4 7 6 * 7 9 3 7 9 3 7 4 6 5 * 4 7 9 4 5 7 5 5 6 7 4 6 9 3 9 3 - - - 3 4 6 9 4 3 6 4 6 5 4 9 5 6 3 8 5 4 6 4 3 5 7 6 4 5 7 - - - 6 4 4 6 7 2 4 4 5 3 9 4 4 3 5 9 3 9 7 2 3 7 8 7 4 3 6 5 5 5 0 2 1 - - - - - - - - 0 4 7 4 - - - - - - - - - - - - - - - - - - - - - - - - - 5 5 4 6 5 2 - - - - - - 3 3 2 M S + + R G C A F L L G V L L A G S L F A F S V A K E E T K K L G K I I G I D L G T T N S + V A V M E G G K P E I I A N + E G V S I G N R T T P S + V A F T + D G E R L V G E P A K + Q A + T N P E + T I F + I K R L + G R K F + D + E V Q R D M K + + P Y K I V + A D G K P + I D + + V K V G + + K + F T P E + I S A M I L Q K + K K T A E A Y L G E + V + + A V I T V P A Y F N D A Q R Q A T K D A G T I A G L N V K R I I N E P T A A A L A Y G L D K + G V G + + + I L V + D L G G G T F D V S I L E I D D V D G E G P V F E V L + T N G D T H L G G E D F D N R I + + Y L + + E F K K K H G I D L R K D + R A L Q R L + D A A E K A K I E L S S + + Q T D I + L P + I T A G + S G P + H + E E T L T R A K F E E L + + D L V E R T + G P V + K A L E D A G L D K S D I + + V I L V G G S T R I P K V Q K + V E D + F + G K E P N K + V N P D E A V A + G A A + Q G G V L S G D + + + E V K D I + L L D V T P L S L G I E T M G G V M T + L I + R N T T I P T K K S Q V F T T A + D N Q T + V T I H V + Q G E R P M A K D N K L L G K F + L + G I P P A P R G + P Q I E V T F D I D A N G I L N V + A + D K G T G K E Q K I T I T + S + G R L S + E E I E R M V + E A E + + A E E D + K R K E R I + A R N Q + E S L V + + + E K T L E E A + K L + D K + + D E D K + K I E + A L + + L E K A L + + N + + A + K E E + E E K M + E L S K V C Q + I + T K + Y Q Q A G G A + G + G + G + + + + + + Q A G P G G M + D M G + + + + + A G A + + N G A A D + G + V D A E F E + V D D D T + K K
TCoffee alignment (Download) {"seqs":[{"name":"UNIPROT|Q97BG8|DNAK_THEVO/1-613","start":1,"end":613,"id":"1008464957","seq":"-------------------------------MSKIIGIDLGTSNSAAAVVISGKPTVIPSSEGVSIGGKAFPSYVAFTKDGQMLVGEPARRQALLNPEGTIFAAKRKMG------------------------TDY-KFKVFD---KEFTPQQISAFILQKIKKDAEAFLGEPVNEAVITVPAYFNDNQRQATKDAGTIAGFDVKRIINEPTAAALAYGVDKSG-KSEKILVFDLGGGTLDVTIIEI---SKRPNVQVLSTSGDTQLGGTDMDEAIVNYIADDFQKKEGIDLRKDRGAYIRLRDAAEKAKIELSTTLSSDIDLPYITVTSSGPKHIKMTLTRAKLEELISPIVERVKAPIDKALEGAKLKKTDITKLLFVGGPTRIPYVRKYVEDYL-GIKAEGGVDPMEAVAIGAAIQGAVLKGE----IKDIVLLDVTPLTLSVETLGGIATPIIPANTTIPVRKSQVFTTAEDMQTTVTIHVVQGERPLAKDNVSLGMFNLTGIAPAPRGIPQIEVTFDIDSNGILNVTAVDKATGKKQGITITASTK-LSKDEIERMKKEAEQYAEQDRKMKEQIETLNNAESLAYSVEKTL---NEAGDKVDKETKDRILSEVKDLRKAIEEKN---MDNVKTLMEKISKDIQEVGTKMYQS----ASST------------------TQTGSGNQNSSKQE-NDKTVDAEYKEKS------","order":1},{"name":"UNIPROT|Q84BU4|Q5FJP4|DNAK_LACAC/1-614","start":1,"end":614,"id":"1738327743","seq":"-------------------------------MSKVIGIDLGTTNSAVAVLEGKEPKIITNPE----GNRTTPSVVAF-KDGEIQVGEVAKRQAITNP-NTIVSIKRHMG-----------------------EADY-KVKVGD---KSYTPQEISAFILQYIKKFSEDYLGEEVKDAVITVPAYFNDAQRQATKDAGKIAGLNVQRIINEPTASALAYGLDKDD-DDEKVLVYDLGGGTFDVSVLQL----GDGVFQVLSTNGDTHLGGDDFDNRIMDWLIKNFKDENGVDLSKDKMAMQRLKDASEKAKKDLSGVSSTHISLPFISAGESGPLHLEADLTRAKFDELTNDLVEKTKIPFDNALKDAGLTVNDIDKVILNGGSTRIPAVQKAVKEWA-GKEPDHSINPDEAVALGAAIQGGVISGD----VKDIVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSKSQIFSTAADNQPAVDVHVLQGERPMAADDKTLGRFELTDIPPAPRGVPQIQVTFDIDKNGIVNVSAKDMGTGKEQKITIKSSSG-LSDEEIKRMQKDAEEHAEEDKKRKDEADLRNEVDQLIFTTEKTL---KETKGKVSDEDTKKVQEALDDLKKAQKDNN---LDEMKEKKDALSKAAQDLAVKLYQQNGGAQGAA------------------GQAGPQGGNPNDGN-NGGAQDGEFHKVDPNK---","order":2},{"name":"UNIPROT|Q56073|DNAK_SALTY/1-638","start":1,"end":638,"id":"730144851","seq":"-------------------------------MGKIIGIDLGTTNSCVAIMDGTQARVLENAE----GDRTTPSIIAYTQDGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPYKIIGADNGDA-WLDVKG---QKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAAALAYGLDKEV-GNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEVLATNGDTHLGGEDFDTRLINYLVDEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDINDVILVGGQTRMPMVQKKVAEFF-GKEPRKDVNPDEAVAIGAAVQGGVLTGD----VKDVLLLDVTPLSLGIETMGGVMTPLITKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRASDNKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSG-LNEEEIQKMVRDAEANAESDRKFEELVQTRNQGDHLLHSTRKQV---EEAGDKLPADDKTAIESALNALETALKGED---KAAIEAKMQELAQVSQKL-MEIAQQ----QHAQ------------------QQAGSADASANNAK-DDDVVDAEFEEVKDKK---","order":3},{"name":"UNIPROT|P11147|Q3KN45|Q8SXQ4|Q9VFB0|HSP7D_DROME/1-651","start":1,"end":651,"id":"1642817950","seq":"-----------------------------MSKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQ----GNRTTPSYVAF-TDTERLIGDAAKNQVAMNPTQTIFDAKRLIGRKFDDAAVQSDMKHWPFEVVSADGKPKIEVTYKD-EKKTFFPEEISSMVLTKMKETAEAYLGKTVTNAVITVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKAVGERNVLIFDLGGGTFDVSILSI----DDGIFEVKSTAGDTHLGGEDFDNRLVTHFVQEFKRKHKKDLTTNKRALRRLRTACERAKRTLSSSTQASIEIDSLFEGT----DFYTSITRARFEELNADLFRSTMDPVEKALRDAKLDKSVIHDIVLVGGSTRIPKVQRLLQDLFNGKELNKSINPDEAVAYGAAVQAAILHGDKSQEVQDLLLLDVTPLSLGIETAGGVMSVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQIEVTFDIDANGILNVTALERSTNKENKITITNDKGRLSKEDIERMVNEAEKYRNEDEKQKETIAAKNGLESYCFNMKATL-DEDNLKTKISDSDRTTILDKCNETIKWLDANQLADKEEYEHRQKELEGVCNPIITKLYQGAGFPPGGMPG----------------GPGGMPGAAGAAGAAGAGGAGPTIEEVD------","order":4},{"name":"UNIPROT|Q6Z7B0|O24182|BIP1_ORYSJ/1-665","start":1,"end":665,"id":"45273986","seq":"MDRVRGCAFLLGVLLAGSLFAFSVAKEETKKLGTVIGIDLGTTYSCVGVYKNGHVEIIANDQ----GNRITPSWVAF-TDSERLIGEAAKNQAAVNPERTIFDVKRLIGRKFEDKEVQRDMKLVPYKIVNKDGKPYIQVKIKDGENKVFSPEEVSAMILGKMKETAEAYLGKKINDAVVTVPAYFNDAQRQATKDAGVIAGLNVARIINEPTAAAIAYGLDKKG-GEKNILVFDLGGGTFDVSILTI----DNGVFEVLATNGDTHLGGEDFDQRIMEYFIKLIKKKYSKDISKDNRALGKLRREAERAKRALSNQHQVRVEIESLFDGT----DFSEPLTRARFEELNNDLFRKTMGPVKKAMDDAGLEKSQIHEIVLVGGSTRIPKVQQLLRDYFEGKEPNKGVNPDEAVAYGAAVQGSILSGEGGDETKDILLLDVAPLTLGIETVGGVMTKLIPRNTVIPTKKSQVFTTYQDQQTTVSIQVFEGERSMTKDCRLLGKFDLSGIPAAPRGTPQIEVTFEVDANGILNVKAEDKGTGKSEKITITNEKGRLSQEEIDRMVREAEEFAEEDKKVKERIDARNQLETYVYNMKNTVGDKDKLADKLESEEKEKVEEALKEALEWLDENQTAEKEEYEEKLKEVEAVCNPIISAVYQR-------------------------------TGGAPGGGADGEGGVDDEHDEL-------","order":5},{"name":"UNIPROT|Q18GZ4|DNAK_HALWD/1-641","start":1,"end":641,"id":"52053077","seq":"-----------------------------MASNKILGIDLGTTNSAFAVMEGDDPEIIVNAE----GDRTTPSVVAMTDDEERLVGKPAKNQVIQNPDQTIRSIKRHMG-----------------------EEDY-TVELGG---EDYTPEQVSAMILQKIKRDAEEYLGDEIEKAVITVPAYFNDRQRQATKDAGEIAGFEVDRIVNEPTAASMAYGLDDES--NQTILVYDLGGGTFDVSVLDL----GGGVYEVVATNGDNDLGGDDWDDAVIDWLAGEFEDNHGIDLRDDRQALQRLKDAAEEAKIELSSRKETTINLPFITATDSGPVHLEETLSRAKFESLTEDLIERTVGPTEQALEDAGYDDSDIDEVILVGGSTRMPQVREKVEDLL-GTEPKKNVNPDEAVALGAAIQGGVLAGD----VDDIVLLDVTPLSLGIEVKGGLFERLIDKNTTIPTEESKVFTTAAANQTSVNVRVFQGEREIAEENELLGEFQLAGIPPAPAGTPQIEVTFNIDENGIVNVEAEDQGSGNAESITIEGGAG-LSDEEIEQMQEEAEAHAEEDERRRERIEARNSAESAVQRAETLL---EENEEDIDDDLKESIEDEVESVEAVLEDED-ATKEEIEDVTESLSSELQEIGKQMYDAQQAAAGAGAGAAGAGAGAGPGGMGDMGDMGDMGGAAGSGDADNEYVDADFEDVDDDTKDE","order":6}],"appSettings":{"globalColorScheme":"Clustal","webStartUrl":"http://www.jalview.org/services/launchApp","application":"Jalview","showSeqFeatures":"false","version":"2.10.1"},"seqGroups":[],"alignAnnotation":[],"svid":"1.0","seqFeatures":[]}
UNIPROT|Q97BG8|DNAK_THEVO/1-613 UNIPROT|Q84BU4|Q5FJP4|DNAK_LACAC/1-614 UNIPROT|Q56073|DNAK_SALTY/1-638 UNIPROT|P11147|Q3KN45|Q8SXQ4|Q9VFB0|HSP7D_DROME/1-651 UNIPROT|Q6Z7B0|O24182|BIP1_ORYSJ/1-665 UNIPROT|Q18GZ4|DNAK_HALWD/1-641 Conservation Quality Consensus
10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 710 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - M S K I I G I D L G T S N S A A A V V I S G K P T V I P S S E G V S I G G K A F P S Y V A F T K D G Q M L V G E P A R R Q A L L N P E G T I F A A K R K M G - - - - - - - - - - - - - - - - - - - - - - - - T D Y - K F K V F D - - - K E F T P Q Q I S A F I L Q K I K K D A E A F L G E P V N E A V I T V P A Y F N D N Q R Q A T K D A G T I A G F D V K R I I N E P T A A A L A Y G V D K S G - K S E K I L V F D L G G G T L D V T I I E I - - - S K R P N V Q V L S T S G D T Q L G G T D M D E A I V N Y I A D D F Q K K E G I D L R K D R G A Y I R L R D A A E K A K I E L S T T L S S D I D L P Y I T V T S S G P K H I K M T L T R A K L E E L I S P I V E R V K A P I D K A L E G A K L K K T D I T K L L F V G G P T R I P Y V R K Y V E D Y L - G I K A E G G V D P M E A V A I G A A I Q G A V L K G E - - - - I K D I V L L D V T P L T L S V E T L G G I A T P I I P A N T T I P V R K S Q V F T T A E D M Q T T V T I H V V Q G E R P L A K D N V S L G M F N L T G I A P A P R G I P Q I E V T F D I D S N G I L N V T A V D K A T G K K Q G I T I T A S T K - L S K D E I E R M K K E A E Q Y A E Q D R K M K E Q I E T L N N A E S L A Y S V E K T L - - - N E A G D K V D K E T K D R I L S E V K D L R K A I E E K N - - - M D N V K T L M E K I S K D I Q E V G T K M Y Q S - - - - A S S T - - - - - - - - - - - - - - - - - - T Q T G S G N Q N S S K Q E - N D K T V D A E Y K E K S - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - M S K V I G I D L G T T N S A V A V L E G K E P K I I T N P E - - - - G N R T T P S V V A F - K D G E I Q V G E V A K R Q A I T N P - N T I V S I K R H M G - - - - - - - - - - - - - - - - - - - - - - - E A D Y - K V K V G D - - - K S Y T P Q E I S A F I L Q Y I K K F S E D Y L G E E V K D A V I T V P A Y F N D A Q R Q A T K D A G K I A G L N V Q R I I N E P T A S A L A Y G L D K D D - D D E K V L V Y D L G G G T F D V S V L Q L - - - - G D G V F Q V L S T N G D T H L G G D D F D N R I M D W L I K N F K D E N G V D L S K D K M A M Q R L K D A S E K A K K D L S G V S S T H I S L P F I S A G E S G P L H L E A D L T R A K F D E L T N D L V E K T K I P F D N A L K D A G L T V N D I D K V I L N G G S T R I P A V Q K A V K E W A - G K E P D H S I N P D E A V A L G A A I Q G G V I S G D - - - - V K D I V L L D V T P L S L G I E T M G G V F T K L I D R N T T I P T S K S Q I F S T A A D N Q P A V D V H V L Q G E R P M A A D D K T L G R F E L T D I P P A P R G V P Q I Q V T F D I D K N G I V N V S A K D M G T G K E Q K I T I K S S S G - L S D E E I K R M Q K D A E E H A E E D K K R K D E A D L R N E V D Q L I F T T E K T L - - - K E T K G K V S D E D T K K V Q E A L D D L K K A Q K D N N - - - L D E M K E K K D A L S K A A Q D L A V K L Y Q Q N G G A Q G A A - - - - - - - - - - - - - - - - - - G Q A G P Q G G N P N D G N - N G G A Q D G E F H K V D P N K - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - M G K I I G I D L G T T N S C V A I M D G T Q A R V L E N A E - - - - G D R T T P S I I A Y T Q D G E T L V G Q P A K R Q A V T N P Q N T L F A I K R L I G R R F Q D E E V Q R D V S I M P Y K I I G A D N G D A - W L D V K G - - - Q K M A P P Q I S A E V L K K M K K T A E D Y L G E P V T E A V I T V P A Y F N D A Q R Q A T K D A G R I A G L E V K R I I N E P T A A A L A Y G L D K E V - G N R T I A V Y D L G G G T F D I S I I E I D E V D G E K T F E V L A T N G D T H L G G E D F D T R L I N Y L V D E F K K D Q G I D L R N D P L A M Q R L K E A A E K A K I E L S S A Q Q T D V N L P Y I T A D A T G P K H M N I K V T R A K L E S L V E D L V N R S I E P L K V A L Q D A G L S V S D I N D V I L V G G Q T R M P M V Q K K V A E F F - G K E P R K D V N P D E A V A I G A A V Q G G V L T G D - - - - V K D V L L L D V T P L S L G I E T M G G V M T P L I T K N T T I P T K H S Q V F S T A E D N Q S A V T I H V L Q G E R K R A S D N K S L G Q F N L D G I N P A P R G M P Q I E V T F D I D A D G I L H V S A K D K N S G K E Q K I T I K A S S G - L N E E E I Q K M V R D A E A N A E S D R K F E E L V Q T R N Q G D H L L H S T R K Q V - - - E E A G D K L P A D D K T A I E S A L N A L E T A L K G E D - - - K A A I E A K M Q E L A Q V S Q K L - M E I A Q Q - - - - Q H A Q - - - - - - - - - - - - - - - - - - Q Q A G S A D A S A N N A K - D D D V V D A E F E E V K D K K - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - M S K A P A V G I D L G T T Y S C V G V F Q H G K V E I I A N D Q - - - - G N R T T P S Y V A F - T D T E R L I G D A A K N Q V A M N P T Q T I F D A K R L I G R K F D D A A V Q S D M K H W P F E V V S A D G K P K I E V T Y K D - E K K T F F P E E I S S M V L T K M K E T A E A Y L G K T V T N A V I T V P A Y F N D S Q R Q A T K D A G T I A G L N V L R I I N E P T A A A I A Y G L D K K A V G E R N V L I F D L G G G T F D V S I L S I - - - - D D G I F E V K S T A G D T H L G G E D F D N R L V T H F V Q E F K R K H K K D L T T N K R A L R R L R T A C E R A K R T L S S S T Q A S I E I D S L F E G T - - - - D F Y T S I T R A R F E E L N A D L F R S T M D P V E K A L R D A K L D K S V I H D I V L V G G S T R I P K V Q R L L Q D L F N G K E L N K S I N P D E A V A Y G A A V Q A A I L H G D K S Q E V Q D L L L L D V T P L S L G I E T A G G V M S V L I K R N T T I P T K Q T Q T F T T Y S D N Q P G V L I Q V Y E G E R A M T K D N N L L G K F E L S G I P P A P R G V P Q I E V T F D I D A N G I L N V T A L E R S T N K E N K I T I T N D K G R L S K E D I E R M V N E A E K Y R N E D E K Q K E T I A A K N G L E S Y C F N M K A T L - D E D N L K T K I S D S D R T T I L D K C N E T I K W L D A N Q L A D K E E Y E H R Q K E L E G V C N P I I T K L Y Q G A G F P P G G M P G - - - - - - - - - - - - - - - - G P G G M P G A A G A A G A A G A G G A G P T I E E V D - - - - - - M D R V R G C A F L L G V L L A G S L F A F S V A K E E T K K L G T V I G I D L G T T Y S C V G V Y K N G H V E I I A N D Q - - - - G N R I T P S W V A F - T D S E R L I G E A A K N Q A A V N P E R T I F D V K R L I G R K F E D K E V Q R D M K L V P Y K I V N K D G K P Y I Q V K I K D G E N K V F S P E E V S A M I L G K M K E T A E A Y L G K K I N D A V V T V P A Y F N D A Q R Q A T K D A G V I A G L N V A R I I N E P T A A A I A Y G L D K K G - G E K N I L V F D L G G G T F D V S I L T I - - - - D N G V F E V L A T N G D T H L G G E D F D Q R I M E Y F I K L I K K K Y S K D I S K D N R A L G K L R R E A E R A K R A L S N Q H Q V R V E I E S L F D G T - - - - D F S E P L T R A R F E E L N N D L F R K T M G P V K K A M D D A G L E K S Q I H E I V L V G G S T R I P K V Q Q L L R D Y F E G K E P N K G V N P D E A V A Y G A A V Q G S I L S G E G G D E T K D I L L L D V A P L T L G I E T V G G V M T K L I P R N T V I P T K K S Q V F T T Y Q D Q Q T T V S I Q V F E G E R S M T K D C R L L G K F D L S G I P A A P R G T P Q I E V T F E V D A N G I L N V K A E D K G T G K S E K I T I T N E K G R L S Q E E I D R M V R E A E E F A E E D K K V K E R I D A R N Q L E T Y V Y N M K N T V G D K D K L A D K L E S E E K E K V E E A L K E A L E W L D E N Q T A E K E E Y E E K L K E V E A V C N P I I S A V Y Q R - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - T G G A P G G G A D G E G G V D D E H D E L - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - M A S N K I L G I D L G T T N S A F A V M E G D D P E I I V N A E - - - - G D R T T P S V V A M T D D E E R L V G K P A K N Q V I Q N P D Q T I R S I K R H M G - - - - - - - - - - - - - - - - - - - - - - - E E D Y - T V E L G G - - - E D Y T P E Q V S A M I L Q K I K R D A E E Y L G D E I E K A V I T V P A Y F N D R Q R Q A T K D A G E I A G F E V D R I V N E P T A A S M A Y G L D D E S - - N Q T I L V Y D L G G G T F D V S V L D L - - - - G G G V Y E V V A T N G D N D L G G D D W D D A V I D W L A G E F E D N H G I D L R D D R Q A L Q R L K D A A E E A K I E L S S R K E T T I N L P F I T A T D S G P V H L E E T L S R A K F E S L T E D L I E R T V G P T E Q A L E D A G Y D D S D I D E V I L V G G S T R M P Q V R E K V E D L L - G T E P K K N V N P D E A V A L G A A I Q G G V L A G D - - - - V D D I V L L D V T P L S L G I E V K G G L F E R L I D K N T T I P T E E S K V F T T A A A N Q T S V N V R V F Q G E R E I A E E N E L L G E F Q L A G I P P A P A G T P Q I E V T F N I D E N G I V N V E A E D Q G S G N A E S I T I E G G A G - L S D E E I E Q M Q E E A E A H A E E D E R R R E R I E A R N S A E S A V Q R A E T L L - - - E E N E E D I D D D L K E S I E D E V E S V E A V L E D E D - A T K E E I E D V T E S L S S E L Q E I G K Q M Y D A Q Q A A A G A G A G A A G A G A G A G P G G M G D M G D M G D M G G A A G S G D A D N E Y V D A D F E D V D D D T K D E - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 3 7 4 7 9 * * * * * * 8 7 * 9 6 + 9 6 3 3 3 4 6 5 9 + 2 9 4 8 - - - - * 5 9 6 7 * * 6 9 * 8 - 5 * 4 8 4 7 9 * 5 6 * 9 7 * 8 7 6 * * 1 4 * + 3 5 7 * * 5 9 * - - - - - - - - - - - - - - - - - - - - - - - 0 3 6 4 - 4 7 5 6 4 5 - - - 6 2 8 5 * 5 8 9 * 8 5 9 * 4 7 9 * 7 4 8 * 4 9 * * 6 3 9 5 5 * * 9 * * * * * * * * 4 * * * * * * * * * 3 * * * 8 7 * 2 * * 9 * * * * * 8 8 9 * * * 9 * 6 4 4 - 0 6 6 6 9 7 9 9 * * * * * * 8 * 9 8 9 + 5 + - - - - 3 3 3 6 6 8 * 5 8 * 7 * * 9 4 * * * 6 * 8 * 6 4 9 8 6 8 8 7 3 4 8 6 6 5 4 4 5 * + 5 5 8 4 3 * 7 3 9 * 9 5 4 7 * 7 * * 5 4 * * 7 3 2 6 6 3 9 6 + 5 5 + 5 3 5 4 - - - - 5 8 3 3 2 9 8 * * 9 8 9 6 * 6 4 6 + 7 6 5 6 5 3 * 6 6 4 * 9 5 5 * 5 7 4 3 8 4 * 4 6 9 9 8 7 * * 6 * * 9 * 3 * 8 6 3 9 3 9 7 6 - * 5 7 5 5 4 5 9 8 * 5 * * * * 7 * * * 9 * + 8 9 + 3 * 9 - - - - 7 5 * 9 9 * * * * 8 * * 8 * 8 9 * 8 4 * * 9 7 5 3 + * 3 4 * * 8 * * 8 4 5 8 7 7 * 8 * 6 4 5 7 * 6 7 * 3 9 6 * 6 8 * * * 2 5 8 3 9 6 3 5 * * 5 * 7 * 5 5 * 6 7 * * 4 * 7 * * * 8 * * * 7 9 * 3 8 * * 9 5 * 4 * 3 9 6 7 8 7 6 3 7 4 * * * 5 7 4 4 5 - * 9 5 9 9 * 5 7 * 4 5 9 * * 3 4 4 7 6 * 7 9 3 7 9 3 7 4 6 5 * 4 7 9 4 5 7 5 5 6 7 4 6 9 - - - 5 5 5 3 4 6 9 4 3 6 4 6 5 4 9 5 6 3 8 5 4 6 4 3 5 7 6 4 5 7 - - - 6 4 4 6 7 2 4 4 5 3 9 4 4 3 5 9 3 9 1 3 3 8 6 7 4 - - - - 0 0 2 0 - - - - - - - - - - - - - - - - - - 0 0 1 3 0 5 5 6 7 6 7 3 6 3 - 5 4 3 5 5 5 4 6 5 5 6 5 1 - - - - - - M D R V R G C A F L L G V L L A G S L F A F S V A K E E T M + M + K I I G I D L G T T N S + V A V M E G G K P E I I A N + E G V S I G N R T T P S + V A F T + D G E R L V G E P A K + Q A + T N P E + T I F + I K R L + G R K F + D + E V Q R D M K + + P Y K I V + A D + K D Y I K V K V K D G E + K + F T P E + I S A M I L Q K + K K T A E A Y L G E + V + + A V I T V P A Y F N D A Q R Q A T K D A G T I A G L N V K R I I N E P T A A A L A Y G L D K + G V G + + + I L V + D L G G G T F D V S I L E I D E V + G D G V F E V L + T N G D T H L G G E D F D N R I + + Y L + + E F K K K H G I D L R K D + R A L Q R L + D A A E K A K I E L S S + + Q T D I + L P + I T A G T S G P K H + E E T L T R A K F E E L + + D L V E R T + G P V + K A L E D A G L D K S D I + + V I L V G G S T R I P K V Q K + V E D + F + G K E P N K + V N P D E A V A + G A A + Q G G V L S G D + + + E V K D I + L L D V T P L S L G I E T M G G V M T + L I + R N T T I P T K K S Q V F T T A + D N Q T + V T I H V + Q G E R P M A K D N K L L G K F + L + G I P P A P R G + P Q I E V T F D I D A N G I L N V + A + D K G T G K E Q K I T I T + S + G R L S + E E I E R M V + E A E + + A E E D + K R K E R I + A R N Q + E S L V + + + E K T L G D + + E + + D K + + D E D K + K I E + A L + + L E K A L + + N + + A + K E E + E E K M + E L S K V C Q + I + T K + Y Q Q + G + A + G A + + G A A G A G A G A G P G G M G D M G Q A G S + G G A + + + G A A + D G G V D A E F E E V D D + K K D E
Muscle alignment (Download) {"seqs":[{"name":"UNIPROT|Q97BG8|DNAK_THEVO/1-613","start":1,"end":613,"id":"970282275","seq":"--MSK-------------------------------IIGIDLGTSNSAAAVVISGKPTVIPSSEGVSIGGKAFPSYVAFTKDGQMLVGEPARRQALLNPEGTIFAAKRKMG--------------TDYKF----------KVFDKE-----FTPQQISAFILQKIKKDAEAFLGEPVNEAVITVPAYFNDNQRQATKDAGTIAGFDVKRIINEPTAAALAYGVDKSG-KSEKILVFDLGGGTLDVTIIEIS---KRPNVQVLSTSGDTQLGGTDMDEAIVNYIADDFQKKEGIDLRKDRGAYIRLRDAAEKAKIELSTTLSSDIDLPYITVTSSGPKHIKMTLTRAKLEELISPIVERVKAPIDKALEGAKLKKTDITKLLFVGGPTRIPYVRKYVEDYL-GIKAEGGVDPMEAVAIGAAIQGAVLKGE----IKDIVLLDVTPLTLSVETLGGIATPIIPANTTIPVRKSQVFTTAEDMQTTVTIHVVQGERPLAKDNVSLGMFNLTGIAPAPRGIPQIEVTFDIDSNGILNVTAVDKATGKKQGITITASTK-LSKDEIERMKKEAEQYAEQDRKMKEQIETLNNAESLAYSVEKTL---NEAGDKVDKETKDRILSEVKD-LRKAIEEKN--MDNVKTLMEKISKDIQEVGTKMYQS----ASS--------TTQTGSGNQNSSKQ-------------------ENDKTVDAEYKEKS------","order":1},{"name":"UNIPROT|Q84BU4|Q5FJP4|DNAK_LACAC/1-614","start":1,"end":614,"id":"1456055067","seq":"--MSK-------------------------------VIGIDLGTTNSAVAVLEGKEPKIITNPE----GNRTTPSVVAF-KDGEIQVGEVAKRQAITNP-NTIVSIKRHMGE-------------ADYKV----------KVGDKS-----YTPQEISAFILQYIKKFSEDYLGEEVKDAVITVPAYFNDAQRQATKDAGKIAGLNVQRIINEPTASALAYGLDKDD-DDEKVLVYDLGGGTFDVSVLQL----GDGVFQVLSTNGDTHLGGDDFDNRIMDWLIKNFKDENGVDLSKDKMAMQRLKDASEKAKKDLSGVSSTHISLPFISAGESGPLHLEADLTRAKFDELTNDLVEKTKIPFDNALKDAGLTVNDIDKVILNGGSTRIPAVQKAVKEWA-GKEPDHSINPDEAVALGAAIQGGVISGD----VKDIVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSKSQIFSTAADNQPAVDVHVLQGERPMAADDKTLGRFELTDIPPAPRGVPQIQVTFDIDKNGIVNVSAKDMGTGKEQKITIKSSSG-LSDEEIKRMQKDAEEHAEEDKKRKDEADLRNEVDQLIFTTEKTL---KETKGKVSDEDTKKVQEALDD-LKKAQKDNN--LDEMKEKKDALSKAAQDLAVKLYQQNGGAQGA--------AGQAGPQGGNPNDG-------------------NNGGAQDGEFHKVDPNK---","order":2},{"name":"UNIPROT|Q56073|DNAK_SALTY/1-638","start":1,"end":638,"id":"871787989","seq":"--MGK-------------------------------IIGIDLGTTNSCVAIMDGTQARVLENAE----GDRTTPSIIAYTQDGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPYKIIGADNGDAWLDVKGQK-----MAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAAALAYGLDKEV-GNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEVLATNGDTHLGGEDFDTRLINYLVDEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDINDVILVGGQTRMPMVQKKVAEFF-GKEPRKDVNPDEAVAIGAAVQGGVLTGD----VKDVLLLDVTPLSLGIETMGGVMTPLITKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRASDNKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSG-LNEEEIQKMVRDAEANAESDRKFEELVQTRNQGDHLLHSTRKQV---EEAGDKLPADDKTAIESALNA-LETALKGED--KAAIEAKMQELAQVSQKL-MEIAQQ----QHA--------QQQAGSADASANNA-------------------KDDDVVDAEFEEVKDKK---","order":3},{"name":"UNIPROT|P11147|Q3KN45|Q8SXQ4|Q9VFB0|HSP7D_DROME/1-651","start":1,"end":651,"id":"1309251174","seq":"--MSK-----------------------------APAVGIDLGTTYSCVGVFQHGKVEIIANDQ----GNRTTPSYVAFT-DTERLIGDAAKNQVAMNPTQTIFDAKRLIGRKFDDAAVQSDMKHWPFEVVSADGKPKI-EVTYKD-EKKTFFPEEISSMVLTKMKETAEAYLGKTVTNAVITVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKAVGERNVLIFDLGGGTFDVSILSI----DDGIFEVKSTAGDTHLGGEDFDNRLVTHFVQEFKRKHKKDLTTNKRALRRLRTACERAKRTLSSSTQASIEIDSLFEGTD----FYTSITRARFEELNADLFRSTMDPVEKALRDAKLDKSVIHDIVLVGGSTRIPKVQRLLQDLFNGKELNKSINPDEAVAYGAAVQAAILHGDKSQEVQDLLLLDVTPLSLGIETAGGVMSVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQIEVTFDIDANGILNVTALERSTNKENKITITNDKGRLSKEDIERMVNEAEKYRNEDEKQKETIAAKNGLESYCFNMKATL-DEDNLKTKISDSDRTTILDKCNETIKWLDANQLADKEEYEHRQKELEGVCNPIITKLYQGAGFPPGGMPGGPGGMPGAAGAAGAAGAGG------------------------AGPTIEEVD------","order":4},{"name":"UNIPROT|Q6Z7B0|O24182|BIP1_ORYSJ/1-665","start":1,"end":665,"id":"1567014601","seq":"--MDRVRGCAFLLGVLLAGSLFAFSVAKEETKKLGTVIGIDLGTTYSCVGVYKNGHVEIIANDQ----GNRITPSWVAFT-DSERLIGEAAKNQAAVNPERTIFDVKRLIGRKFEDKEVQRDMKLVPYKIVNKDGKPYI-QVKIKDGENKVFSPEEVSAMILGKMKETAEAYLGKKINDAVVTVPAYFNDAQRQATKDAGVIAGLNVARIINEPTAAAIAYGLDKKG-GEKNILVFDLGGGTFDVSILTI----DNGVFEVLATNGDTHLGGEDFDQRIMEYFIKLIKKKYSKDISKDNRALGKLRREAERAKRALSNQHQVRVEIESLFDGTD----FSEPLTRARFEELNNDLFRKTMGPVKKAMDDAGLEKSQIHEIVLVGGSTRIPKVQQLLRDYFEGKEPNKGVNPDEAVAYGAAVQGSILSGEGGDETKDILLLDVAPLTLGIETVGGVMTKLIPRNTVIPTKKSQVFTTYQDQQTTVSIQVFEGERSMTKDCRLLGKFDLSGIPAAPRGTPQIEVTFEVDANGILNVKAEDKGTGKSEKITITNEKGRLSQEEIDRMVREAEEFAEEDKKVKERIDARNQLETYVYNMKNTVGDKDKLADKLESEEKEKVEEALKEALEWLDENQTAEKEEYEEKLKEVEAVCNPIISAVYQRTGGAPGG---------------GADGEGG------------------------VDDEHDEL-------","order":5},{"name":"UNIPROT|Q18GZ4|DNAK_HALWD/1-641","start":1,"end":641,"id":"1177292549","seq":"MASNK-------------------------------ILGIDLGTTNSAFAVMEGDDPEIIVNAE----GDRTTPSVVAMTDDEERLVGKPAKNQVIQNPDQTIRSIKRHMGE-------------EDYTV----------ELGGED-----YTPEQVSAMILQKIKRDAEEYLGDEIEKAVITVPAYFNDRQRQATKDAGEIAGFEVDRIVNEPTAASMAYGLD-DE-SNQTILVYDLGGGTFDVSVLDL----GGGVYEVVATNGDNDLGGDDWDDAVIDWLAGEFEDNHGIDLRDDRQALQRLKDAAEEAKIELSSRKETTINLPFITATDSGPVHLEETLSRAKFESLTEDLIERTVGPTEQALEDAGYDDSDIDEVILVGGSTRMPQVREKVEDLL-GTEPKKNVNPDEAVALGAAIQGGVLAGD----VDDIVLLDVTPLSLGIEVKGGLFERLIDKNTTIPTEESKVFTTAAANQTSVNVRVFQGEREIAEENELLGEFQLAGIPPAPAGTPQIEVTFNIDENGIVNVEAEDQGSGNAESITIEGGAG-LSDEEIEQMQEEAEAHAEEDERRRERIEARNSAESAVQRAETLL---EENEEDIDDDLKESIEDEVES-VEAVLEDEDATKEEIEDVTESLSSELQEIGKQMYDAQQAAAGA--------GAGAAGAGAGAGPGGMGDMGDMGDMGGAAGSGDADNEYVDADFEDVDDDTKDE","order":6}],"appSettings":{"globalColorScheme":"Clustal","webStartUrl":"http://www.jalview.org/services/launchApp","application":"Jalview","showSeqFeatures":"false","version":"2.10.1"},"seqGroups":[],"alignAnnotation":[],"svid":"1.0","seqFeatures":[]}
UNIPROT|Q97BG8|DNAK_THEVO/1-613 UNIPROT|Q84BU4|Q5FJP4|DNAK_LACAC/1-614 UNIPROT|Q56073|DNAK_SALTY/1-638 UNIPROT|P11147|Q3KN45|Q8SXQ4|Q9VFB0|HSP7D_DROME/1-651 UNIPROT|Q6Z7B0|O24182|BIP1_ORYSJ/1-665 UNIPROT|Q18GZ4|DNAK_HALWD/1-641 Conservation Quality Consensus
10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 710 720 - - M S K - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - I I G I D L G T S N S A A A V V I S G K P T V I P S S E G V S I G G K A F P S Y V A F T K D G Q M L V G E P A R R Q A L L N P E G T I F A A K R K M G - - - - - - - - - - - - - - T D Y K F - - - - - - - - - - K V F D K E - - - - - F T P Q Q I S A F I L Q K I K K D A E A F L G E P V N E A V I T V P A Y F N D N Q R Q A T K D A G T I A G F D V K R I I N E P T A A A L A Y G V D K S G - K S E K I L V F D L G G G T L D V T I I E I S - - - K R P N V Q V L S T S G D T Q L G G T D M D E A I V N Y I A D D F Q K K E G I D L R K D R G A Y I R L R D A A E K A K I E L S T T L S S D I D L P Y I T V T S S G P K H I K M T L T R A K L E E L I S P I V E R V K A P I D K A L E G A K L K K T D I T K L L F V G G P T R I P Y V R K Y V E D Y L - G I K A E G G V D P M E A V A I G A A I Q G A V L K G E - - - - I K D I V L L D V T P L T L S V E T L G G I A T P I I P A N T T I P V R K S Q V F T T A E D M Q T T V T I H V V Q G E R P L A K D N V S L G M F N L T G I A P A P R G I P Q I E V T F D I D S N G I L N V T A V D K A T G K K Q G I T I T A S T K - L S K D E I E R M K K E A E Q Y A E Q D R K M K E Q I E T L N N A E S L A Y S V E K T L - - - N E A G D K V D K E T K D R I L S E V K D - L R K A I E E K N - - M D N V K T L M E K I S K D I Q E V G T K M Y Q S - - - - A S S - - - - - - - - T T Q T G S G N Q N S S K Q - - - - - - - - - - - - - - - - - - - E N D K T V D A E Y K E K S - - - - - - - - M S K - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - V I G I D L G T T N S A V A V L E G K E P K I I T N P E - - - - G N R T T P S V V A F - K D G E I Q V G E V A K R Q A I T N P - N T I V S I K R H M G E - - - - - - - - - - - - - A D Y K V - - - - - - - - - - K V G D K S - - - - - Y T P Q E I S A F I L Q Y I K K F S E D Y L G E E V K D A V I T V P A Y F N D A Q R Q A T K D A G K I A G L N V Q R I I N E P T A S A L A Y G L D K D D - D D E K V L V Y D L G G G T F D V S V L Q L - - - - G D G V F Q V L S T N G D T H L G G D D F D N R I M D W L I K N F K D E N G V D L S K D K M A M Q R L K D A S E K A K K D L S G V S S T H I S L P F I S A G E S G P L H L E A D L T R A K F D E L T N D L V E K T K I P F D N A L K D A G L T V N D I D K V I L N G G S T R I P A V Q K A V K E W A - G K E P D H S I N P D E A V A L G A A I Q G G V I S G D - - - - V K D I V L L D V T P L S L G I E T M G G V F T K L I D R N T T I P T S K S Q I F S T A A D N Q P A V D V H V L Q G E R P M A A D D K T L G R F E L T D I P P A P R G V P Q I Q V T F D I D K N G I V N V S A K D M G T G K E Q K I T I K S S S G - L S D E E I K R M Q K D A E E H A E E D K K R K D E A D L R N E V D Q L I F T T E K T L - - - K E T K G K V S D E D T K K V Q E A L D D - L K K A Q K D N N - - L D E M K E K K D A L S K A A Q D L A V K L Y Q Q N G G A Q G A - - - - - - - - A G Q A G P Q G G N P N D G - - - - - - - - - - - - - - - - - - - N N G G A Q D G E F H K V D P N K - - - - - M G K - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - I I G I D L G T T N S C V A I M D G T Q A R V L E N A E - - - - G D R T T P S I I A Y T Q D G E T L V G Q P A K R Q A V T N P Q N T L F A I K R L I G R R F Q D E E V Q R D V S I M P Y K I I G A D N G D A W L D V K G Q K - - - - - M A P P Q I S A E V L K K M K K T A E D Y L G E P V T E A V I T V P A Y F N D A Q R Q A T K D A G R I A G L E V K R I I N E P T A A A L A Y G L D K E V - G N R T I A V Y D L G G G T F D I S I I E I D E V D G E K T F E V L A T N G D T H L G G E D F D T R L I N Y L V D E F K K D Q G I D L R N D P L A M Q R L K E A A E K A K I E L S S A Q Q T D V N L P Y I T A D A T G P K H M N I K V T R A K L E S L V E D L V N R S I E P L K V A L Q D A G L S V S D I N D V I L V G G Q T R M P M V Q K K V A E F F - G K E P R K D V N P D E A V A I G A A V Q G G V L T G D - - - - V K D V L L L D V T P L S L G I E T M G G V M T P L I T K N T T I P T K H S Q V F S T A E D N Q S A V T I H V L Q G E R K R A S D N K S L G Q F N L D G I N P A P R G M P Q I E V T F D I D A D G I L H V S A K D K N S G K E Q K I T I K A S S G - L N E E E I Q K M V R D A E A N A E S D R K F E E L V Q T R N Q G D H L L H S T R K Q V - - - E E A G D K L P A D D K T A I E S A L N A - L E T A L K G E D - - K A A I E A K M Q E L A Q V S Q K L - M E I A Q Q - - - - Q H A - - - - - - - - Q Q Q A G S A D A S A N N A - - - - - - - - - - - - - - - - - - - K D D D V V D A E F E E V K D K K - - - - - M S K - - - - - - - - - - - - - - - - - - - - - - - - - - - - - A P A V G I D L G T T Y S C V G V F Q H G K V E I I A N D Q - - - - G N R T T P S Y V A F T - D T E R L I G D A A K N Q V A M N P T Q T I F D A K R L I G R K F D D A A V Q S D M K H W P F E V V S A D G K P K I - E V T Y K D - E K K T F F P E E I S S M V L T K M K E T A E A Y L G K T V T N A V I T V P A Y F N D S Q R Q A T K D A G T I A G L N V L R I I N E P T A A A I A Y G L D K K A V G E R N V L I F D L G G G T F D V S I L S I - - - - D D G I F E V K S T A G D T H L G G E D F D N R L V T H F V Q E F K R K H K K D L T T N K R A L R R L R T A C E R A K R T L S S S T Q A S I E I D S L F E G T D - - - - F Y T S I T R A R F E E L N A D L F R S T M D P V E K A L R D A K L D K S V I H D I V L V G G S T R I P K V Q R L L Q D L F N G K E L N K S I N P D E A V A Y G A A V Q A A I L H G D K S Q E V Q D L L L L D V T P L S L G I E T A G G V M S V L I K R N T T I P T K Q T Q T F T T Y S D N Q P G V L I Q V Y E G E R A M T K D N N L L G K F E L S G I P P A P R G V P Q I E V T F D I D A N G I L N V T A L E R S T N K E N K I T I T N D K G R L S K E D I E R M V N E A E K Y R N E D E K Q K E T I A A K N G L E S Y C F N M K A T L - D E D N L K T K I S D S D R T T I L D K C N E T I K W L D A N Q L A D K E E Y E H R Q K E L E G V C N P I I T K L Y Q G A G F P P G G M P G G P G G M P G A A G A A G A A G A G G - - - - - - - - - - - - - - - - - - - - - - - - A G P T I E E V D - - - - - - - - M D R V R G C A F L L G V L L A G S L F A F S V A K E E T K K L G T V I G I D L G T T Y S C V G V Y K N G H V E I I A N D Q - - - - G N R I T P S W V A F T - D S E R L I G E A A K N Q A A V N P E R T I F D V K R L I G R K F E D K E V Q R D M K L V P Y K I V N K D G K P Y I - Q V K I K D G E N K V F S P E E V S A M I L G K M K E T A E A Y L G K K I N D A V V T V P A Y F N D A Q R Q A T K D A G V I A G L N V A R I I N E P T A A A I A Y G L D K K G - G E K N I L V F D L G G G T F D V S I L T I - - - - D N G V F E V L A T N G D T H L G G E D F D Q R I M E Y F I K L I K K K Y S K D I S K D N R A L G K L R R E A E R A K R A L S N Q H Q V R V E I E S L F D G T D - - - - F S E P L T R A R F E E L N N D L F R K T M G P V K K A M D D A G L E K S Q I H E I V L V G G S T R I P K V Q Q L L R D Y F E G K E P N K G V N P D E A V A Y G A A V Q G S I L S G E G G D E T K D I L L L D V A P L T L G I E T V G G V M T K L I P R N T V I P T K K S Q V F T T Y Q D Q Q T T V S I Q V F E G E R S M T K D C R L L G K F D L S G I P A A P R G T P Q I E V T F E V D A N G I L N V K A E D K G T G K S E K I T I T N E K G R L S Q E E I D R M V R E A E E F A E E D K K V K E R I D A R N Q L E T Y V Y N M K N T V G D K D K L A D K L E S E E K E K V E E A L K E A L E W L D E N Q T A E K E E Y E E K L K E V E A V C N P I I S A V Y Q R T G G A P G G - - - - - - - - - - - - - - - G A D G E G G - - - - - - - - - - - - - - - - - - - - - - - - V D D E H D E L - - - - - - - M A S N K - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - I L G I D L G T T N S A F A V M E G D D P E I I V N A E - - - - G D R T T P S V V A M T D D E E R L V G K P A K N Q V I Q N P D Q T I R S I K R H M G E - - - - - - - - - - - - - E D Y T V - - - - - - - - - - E L G G E D - - - - - Y T P E Q V S A M I L Q K I K R D A E E Y L G D E I E K A V I T V P A Y F N D R Q R Q A T K D A G E I A G F E V D R I V N E P T A A S M A Y G L D - D E - S N Q T I L V Y D L G G G T F D V S V L D L - - - - G G G V Y E V V A T N G D N D L G G D D W D D A V I D W L A G E F E D N H G I D L R D D R Q A L Q R L K D A A E E A K I E L S S R K E T T I N L P F I T A T D S G P V H L E E T L S R A K F E S L T E D L I E R T V G P T E Q A L E D A G Y D D S D I D E V I L V G G S T R M P Q V R E K V E D L L - G T E P K K N V N P D E A V A L G A A I Q G G V L A G D - - - - V D D I V L L D V T P L S L G I E V K G G L F E R L I D K N T T I P T E E S K V F T T A A A N Q T S V N V R V F Q G E R E I A E E N E L L G E F Q L A G I P P A P A G T P Q I E V T F N I D E N G I V N V E A E D Q G S G N A E S I T I E G G A G - L S D E E I E Q M Q E E A E A H A E E D E R R R E R I E A R N S A E S A V Q R A E T L L - - - E E N E E D I D D D L K E S I E D E V E S - V E A V L E D E D A T K E E I E D V T E S L S S E L Q E I G K Q M Y D A Q Q A A A G A - - - - - - - - G A G A A G A G A G A G P G G M G D M G D M G D M G G A A G S G D A D N E Y V D A D F E D V D D D T K D E - - 6 5 9 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 7 9 * * * * * * 8 7 * 9 6 + 9 6 3 3 3 4 6 5 9 + 2 9 4 8 - - - - * 5 9 6 7 * * 6 9 * 8 3 - * 4 8 4 7 9 * 5 6 * 9 7 * 8 7 6 * * 1 4 * + 3 5 7 * * 5 9 * 2 - - - - - - - - - - - - - 2 6 9 5 7 - - - - - - - - - - 5 9 4 2 6 4 - - - - - 8 5 * 5 8 9 * 8 5 9 * 4 7 9 * 7 4 8 * 4 9 * * 6 3 9 5 5 * * 9 * * * * * * * * 4 * * * * * * * * * 3 * * * 8 7 * 2 * * 9 * * * * * 8 8 9 * * * 9 * 4 4 3 - 3 6 6 6 9 7 9 9 * * * * * * 8 * 9 8 9 + 5 + - - - - 3 3 3 6 6 8 * 5 8 * 7 * * 9 4 * * * 6 * 8 * 6 4 9 8 6 8 8 7 3 4 8 6 6 5 4 4 5 * + 5 5 8 4 3 * 7 3 9 * 9 5 4 7 * 7 * * 5 4 * * 7 3 2 6 6 3 9 6 + 5 5 + 5 3 5 4 6 - - - - 8 3 3 2 9 8 * * 9 8 9 6 * 6 4 6 + 7 6 5 6 5 3 * 6 6 4 * 9 5 5 * 5 7 4 3 8 4 * 4 6 9 9 8 7 * * 6 * * 9 * 3 * 8 6 3 9 3 9 7 6 - * 5 7 5 5 4 5 9 8 * 5 * * * * 7 * * * 9 * + 8 9 + 3 * 9 - - - - 7 5 * 9 9 * * * * 8 * * 8 * 8 9 * 8 4 * * 9 7 5 3 + * 3 4 * * 8 * * 8 4 5 8 7 7 * 8 * 6 4 5 7 * 6 7 * 3 9 6 * 6 8 * * * 2 5 8 3 9 6 3 5 * * 5 * 7 * 5 5 * 6 7 * * 4 * 7 * * * 8 * * * 7 9 * 3 8 * * 9 5 * 4 * 3 9 6 7 8 7 6 3 7 4 * * * 5 7 4 4 5 - * 9 5 9 9 * 5 7 * 4 5 9 * * 3 4 4 7 6 * 7 9 3 7 9 3 7 4 6 5 * 4 7 9 4 5 7 5 5 6 7 4 6 9 - - - 5 5 5 3 4 6 9 4 3 6 4 6 5 4 9 5 6 3 8 5 4 - 9 7 4 7 4 3 4 5 4 - - 6 4 4 6 7 2 4 4 5 3 9 4 4 3 5 9 3 9 1 3 3 8 6 7 4 - - - - 5 3 8 - - - - - - - - 0 0 0 2 3 1 0 5 6 5 6 4 2 6 - - - - - - - - - - - - - - - - - - - - - - - - 5 5 4 6 5 5 6 5 1 - - - - - - M A M S K V R G C A F L L G V L L A G S L F A F S V A K E E T K K L + + I I G I D L G T T N S + V A V M E G G K P E I I A N + E G V S I G N R T T P S + V A F T K D G E R L V G E P A K + Q A + T N P E + T I F + I K R L + G R K F + D + E V Q R D M K + + + Y K V V + A D G K P + I L + V + + K D G E + K + F T P E + I S A M I L Q K + K K T A E A Y L G E + V + + A V I T V P A Y F N D A Q R Q A T K D A G T I A G L N V K R I I N E P T A A A L A Y G L D K + G V G + + + I L V + D L G G G T F D V S I L E I + E V D G D G V F E V L + T N G D T H L G G E D F D N R I + + Y L + + E F K K K H G I D L R K D + R A L Q R L + D A A E K A K I E L S S + + Q T D I + L P + I T A G T S G P K H + E E T L T R A K F E E L + + D L V E R T + G P V + K A L E D A G L D K S D I + + V I L V G G S T R I P K V Q K + V E D + F + G K E P N K + V N P D E A V A + G A A + Q G G V L S G D + + + E V K D I + L L D V T P L S L G I E T M G G V M T + L I + R N T T I P T K K S Q V F T T A + D N Q T + V T I H V + Q G E R P M A K D N K L L G K F + L + G I P P A P R G + P Q I E V T F D I D A N G I L N V + A + D K G T G K E Q K I T I T + S + G R L S + E E I E R M V + E A E + + A E E D + K R K E R I + A R N Q + E S L V + + + E K T L G D + + E + + D K + + D E D K + K I E + A L + + + L E + A + E + + + A + K E E + E E K M + E L S K V C Q + I + T K + Y Q Q + G G A + G A M P G G P G G M + G Q A G S A G A N + N G G G M G D M G D M G D M G G A A G S G D + + D + + V D A E F E E V D D + K K D E
Mafft alignment (Download) webPRANK alignment
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Comparing of Muscle and Mafft alignments (noticed differences)
Lysine (K) #3 (sequence #3) Muscle: stands in the position with 3 more K (lysine #3, #3, #5), P (proline #5) and T (threonine #34)Mafft: stands in the position with 4 more K (lysine) and R (arginine #3)Glutamic acid (E) #73 (sequence #2) Muscle: stands in the position with N (asparagine #98), 2 G (glycine #99, #128), E (glutamic acid #77)Mafft: stands in the position with 3 R (arginine #75, #76 , #105), E (glutamic acid #77)Tyrosine (Y) #81 (sequence #1) Muscle: stands in the position with 3 more Y (tyrosine #76, #131, #80), A (alanine #101), K (lysine #102)Mafft: stands in the position with 4 more Y (tyrosine #76, #91, #121, #80), F (phenylalanine #92)
Task 2
Figure 1. Protein domain architectureFigure 2. Pfam data
Table 3. Description of 3 differents architectures with Bac_globin domain
Protein Amount of sequences Description
1
This protein consists of Bac_globin only as mine too, however, there is 5 Bac_globins, with deletions in the first, second and the fifth.
Bacterial-like globin (Bac_globin) - the family of heme binding proteins, which are found mainly in bacteria. According to the Pfam article , they can also be found in some protozoa and plants.
Molecular function: oxygen binding
Putative uncharacterized protein (Perkinsus marinus)
1
Bac_globin: Deletion on the right
Alpha Amylase: According to the Pfam article , enzymes containing this domain belong to family 13 (CAZY GH_13) of the glycosyl hydrolases (it is a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate part).
Molecular function: catalytic activity
Group 2 hemoglobin glbO (Mycobacterium xenopi)
1
Bac_globin:
Cytochrome P460: There is no Pfam article about this domain yet, so my description of it's function was based on NCBI article.[2] Molecular function: catalysation of the oxidation of hydroxylamine to nitrite
Hemoglobin-like protein HbN (Nitrospira moscoviensis)
References
Multiple Sequence Alignment "Cytochrome P460 Genes from the Methanotroph Methylococcus capsulatus Bath." J Bacteriol. 1998 Dec; 180(24): 6440–6445.
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