Proteomes

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During this task, I had to learn how to use 'Proteomes' UniProt section and compare proteome of Escherichia coli K12 with my organism's one.
All results are listed below.

Table 1.General information
Name: Escherichia coli K12 Streptomyces avermitilis MA-4680
Proteome ID: UP000000625 UP000000428
Amount of sequnces: 4306 7672
Amount of residues: 1356195 2610487

Results

Table 2. Comparing of Escherichia coli K12 and Streptomyces avermitilis MA-4680
Residues Streptomyces avermitilis MA-4680, % Escherichia coli K12, % Δ, %
A 13,4% 9,5% 3,9%
L 10,2% 10,7% -0,5%
G 9,5% 7,4% 2,1%
V 8,3% 7,1% 1,2%
R 8,0% 5,5% 2,5%
T 6,2% 5,4% 0,8%
P 6,1% 4,4% 1,7%
D 5,9% 5,2% 0,7%
E 5,6% 5,8% -0,2%
S 5,3% 5,8% -0,5%
I 3,1% 6,0% -2,9%
Q 2,9% 4,4% -1,5%
F 2,7% 3,9% -1,2%
H 2,3% 2,3% 0,0%
K 2,3% 4,4% -2,1%
Y 2,1% 2,8% -0,7%
N 1,8% 3,9% -2,1%
M 1,7% 2,8% -1,1%
W 1,5% 1,5% 0,0%
C 0,8% 1,2% -0,4%
Table 3. Table 2 analysis
Organism Escherichia coli K12 Streptomyces avermitilis MA-4680
Three most common residues Leu, Ala, Gly Ala, Leu, Gly
Three rarest residues Cys, Trp, His Cys, Trp, Met
The biggest difference in amount of residues, % Ile, 2.9 Ala, 3.9

The most common residues are the same, but there are more leucine residues, than alanine residues in E.coli.
The first positions in 'three rarest residues' category are the same, however, E.coli proteome has more metionine residues, than S.avermitilis proteome.
Despite the fact that the proteome of E.coli is almost 2 times smaller than the S.avermitilis proteome, it doesn't mean that it has to contain less amounts of every residue. For example, E. coli proteome has 3.9% more isoleucine residues, than S.avermitilis's one.

Used programms


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© Sophia Veselova, 2017.