Nucleotide BLAST
Nucleotide BLAST
Task 1
Taxonomy: Eukaryota; Metazoa; Echinodermata; Eleutherozoa; Asterozoa;
Ophiuroidea; Ophiuridea; Ophiurida; Ophiurina; Gnathophiurina;
Ophiactidae; Ophiopholis; Ophiopholis aculeata
Function: Mitochondrial gene (cytochrome oxidase
subunit 1 (COI) gene, partial cds; mitochondrial)
Task 2
BLAST parameters:
| Algorithm | E-value | Max target sequences | Word size | Hits |
| megablast | 0.001 | 10000 | 28 | 287 |
| discontiguous megablast | 0.001 | 10000 | 11 | 6009 |
| blastn | 0.001 | 10000 | 15 | 4211 |
Results
Gorgonocephalus chilensis voucher SIO:BIC:E6110 cytochrome oxidase subunit I (COI) gene, partial cds; mitochondrial (link) - wasn't found by megablast
Task 3
Used algorithm: tblastn
- TBB_NEUCR - was found an ortholog;
- HSP71_YEAST - was found a homolog;
- EIF3G_SCHPO - slight similarity;
Task 4
Scaffold-499 was used to find (with the help of blastx) a gene which encodes bifunctional purine biosynthesis protein ADE17.
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© Sophia Veselova, 2017.