FastQCFastQC Report
Сб 8 дек 2018
chr5.1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamechr5.1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24156
Sequences flagged as poor quality0
Sequence length41-51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCACCATTTCCATGTCTGACCACCGCTACTACTATGTCCTATCATAA10074.168736545785726No Hit
CCACCATTTCCATGTCTGACCACCGCTACTACTATGTCCTATCATAACATT9904.098360655737705No Hit
CTCTGCTTCAACAATGTGCAACTCATCCTTTGCACCAGCCCCTAAACTGAC8153.3739029640668985No Hit
CACATGTATATTTTTGTCTCCCCACCATTTCCATGTCTGACCACCGCTACT7403.0634210962079815No Hit
CCCACCATTTCCATGTCTGACCACCGCTACTACTATGTCCTATCATAACAT6012.487994701109455No Hit
CACCATTTCCATGTCTGACCACCGCTACTACTATGTCCTATCATAACATTC6002.4838549428713366No Hit
CTCCTCTTCATCTTCTGACTCTGCATCTTCCTCCACAGCTACTAAGTGCTG5962.4672959099188607No Hit
CTTCGTAATTCATTGCCTCTGCTTCAACAATGTGCAACTCATCCTTTGCAC5872.4300380857757906No Hit
CTTCTACAGAACTAGGTCCTTTTGGTGTTTTAGGAGTTTTTTCCTGTTTCT5002.069879119059447No Hit
CTCCTCCTCTTCATCTTCTGACTCTGCATCTTCCTCCACAGCTACTAAGTG4922.0367610531544957No Hit
GTTTTAGGAGTTTTTTCCTGTTTCTTGAAGGATTCTTGTCCTTTTGATCTT4421.8297731412485512No Hit
CCCCACCATTTCCATGTCTGACCACCGCTACTACTATGTCCTATCATAACA4061.6807418446762707No Hit
CTTCATCATCATCGTCATCATCTTCATCAGCAGCAAGTTTTACTTTTTTCT3611.4944527239609207No Hit
GTCCTTTTGGTGTTTTAGGAGTTTTTTCCTGTTTCTTGAAGGATTCTTGTC3541.4654744162940885No Hit
GGCGATTTAAAACATTGTCAGGTGAGGCAAATGCACAAGTAATAGAAAGCA3251.3454214273886405No Hit
ATTTTTGTCTCCCCACCATTTCCATGTCTGACCACCGCTACTACTATGTCC3131.2957443285312138No Hit
CTGCCTTCGTAATTCATTGCCTCTGCTTCAACAATGTGCAACTCATCCTTT3041.2584865043881437No Hit
GTATATTTTTGTCTCCCCACCATTTCCATGTCTGACCACCGCTACTACTAT3011.2460672296737871No Hit
GTCCTTTTGATCTTGGTGTTGATGATGGTTTTGAGTCTTTTCCATTCTGAT2981.2336479549594304No Hit
CCTGTTTCTTGAAGGATTCTTGTCCTTTTGATCTTGGTGTTGATGATGGTT2280.9438648782911079No Hit
GTCATCATCTTCATCAGCAGCAAGTTTTACTTTTTTCTGTGGAACCTTGCT2270.9397251200529888No Hit
CCCAAGGGAAACCGTTGGCTGTACAGACATTTTCAAAGTTGCCAGTGTTAC2170.8983275376718No Hit
CTTAAAGTGATAATCTTTGTCGGCCTTTAGTTCACAACCGAAAAGATAGTT2010.8320914058618977No Hit
CTACAGAACTAGGTCCTTTTGGTGTTTTAGGAGTTTTTTCCTGTTTCTTGA1880.778274548766352No Hit
GTTTCTTGAAGGATTCTTGTCCTTTTGATCTTGGTGTTGATGATGGTTTTG1860.7699950322901143No Hit
GCCTCTGCTTCAACAATGTGCAACTCATCCTTTGCACCAGCCCCTAAACTG1830.7575757575757576No Hit
ATATTTTTGTCTCCCCACCATTTCCATGTCTGACCACCGCTACTACTATGT1730.7161781751945686No Hit
GGACTGCCTTCGTAATTCATTGCCTCTGCTTCAACAATGTGCAACTCATCC1720.7120384169564498No Hit
GTGTTTTAGGAGTTTTTTCCTGTTTCTTGAAGGATTCTTGTCCTTTTGATC1700.703758900480212No Hit
GTGCATTTTTGGCTGGAGTATCTCGTATAGATTTCTTCACTGGCGCTTTTT1670.6913396257658553No Hit
ACCATTTCCATGTCTGACCACCGCTACTACTATGTCCTATCATAACATTCC1660.6871998675277364No Hit
CTCATTTTCATCATTATCCACCTTAAAGTGATAATCTTTGTCGGCCTTTAG1630.6747805928133798No Hit
CGTAATTCATTGCCTCTGCTTCAACAATGTGCAACTCATCCTTTGCACCAG1570.6499420433846663No Hit
CATGTATATTTTTGTCTCCCCACCATTTCCATGTCTGACCACCGCTACTAC1400.5795661533366452No Hit
GGCGCTTTTTCTTCAGCTTCCTCATCATCAAAATCATCATCATCATCATCT1380.5712866368604074No Hit
CCACCTTAAAGTGATAATCTTTGTCGGCCTTTAGTTCACAACCGAAAAGAT1350.5588673621460507No Hit
CCGTTCTTAAAGATAACTGGTGCTCATTTTCATCATTATCCACCTTAAAGT1320.546448087431694No Hit
TTCGTAATTCATTGCCTCTGCTTCAACAATGTGCAACTCATCCTTTGCACC1290.5340288127173373No Hit
CTCGTATAGATTTCTTCACTGGCGCTTTTTCTTCAGCTTCCTCATCATCAA1250.5174697797648617No Hit
TCGTAATTCATTGCCTCTGCTTCAACAATGTGCAACTCATCCTTTGCACCA1240.5133300215267428No Hit
GCCTTCGTAATTCATTGCCTCTGCTTCAACAATGTGCAACTCATCCTTTGC1220.5050505050505051No Hit
CCTTAAAGTGATAATCTTTGTCGGCCTTTAGTTCACAACCGAAAAGATAGT1220.5050505050505051No Hit
TACAGAACTAGGTCCTTTTGGTGTTTTAGGAGTTTTTTCCTGTTTCTTGAA1180.4884914720980295No Hit
GTAATTCATTGCCTCTGCTTCAACAATGTGCAACTCATCCTTTGCACCAGC1170.48435171385991055No Hit
GCTCATTTTCATCATTATCCACCTTAAAGTGATAATCTTTGTCGGCCTTTA1010.41811558205000826No Hit
GTCAGGTGAGGCAAATGCACAAGTAATAGAAAGCAAAGGGCAAGGTTCACT990.4098360655737705No Hit
CTTCATCTTCTGACTCTGCATCTTCCTCCACAGCTACTAAGTGCTGTCCAC990.4098360655737705No Hit
ATCGTCATCATCTTCATCAGCAGCAAGTTTTACTTTTTTCTGTGGAACCTT950.39327703262129493No Hit
GACCGTTCTTAAAGATAACTGGTGCTCATTTTCATCATTATCCACCTTAAA940.389137274383176No Hit
GCGCTTTTTCTTCAGCTTCCTCATCATCAAAATCATCATCATCATCATCTT900.37257824143070045No Hit
TTTAGGAGTTTTTTCCTGTTTCTTGAAGGATTCTTGTCCTTTTGATCTTGG900.37257824143070045No Hit
TCTTCATCATCATCGTCATCATCTTCATCAGCAGCAAGTTTTACTTTTTTC890.3684384831925816No Hit
GTGAGGCAAATGCACAAGTAATAGAAAGCAAAGGGCAAGGTTCACTGAATC860.3560192084782249No Hit
TGCCTTCGTAATTCATTGCCTCTGCTTCAACAATGTGCAACTCATCCTTTG860.3560192084782249No Hit
GTCAGTCATCCGGAAGCAATTCTTCACATAATTGATGAATTTGGCTTCCAC830.3435999337638682No Hit
CTTTTGATCTTGGTGTTGATGATGGTTTTGAGTCTTTTCCATTCTGATTTG820.3394601755257493No Hit
TGTTTTAGGAGTTTTTTCCTGTTTCTTGAAGGATTCTTGTCCTTTTGATCT780.32290114257327374No Hit
ATCCACCTTAAAGTGATAATCTTTGTCGGCCTTTAGTTCACAACCGAAAAG770.31876138433515483No Hit
CCGCCATTTCCATGTCTGACCACCGCTACTACTATGTCCTATCATAACATT750.31048186785891707No Hit
CTTCAACAATGTGCAACTCATCCTTTGCACCAGCCCCTAAACTGACCGTTC740.30634210962079816No Hit
CCCATCTTTGGGCTTAATTTACATGTAGTGCCCAGGACTGTTCAATGCGCT730.30220235138267926No Hit
TGTTTCTTGAAGGATTCTTGTCCTTTTGATCTTGGTGTTGATGATGGTTTT710.2939228349064415No Hit
CCTAAACTGACCGTTCTTAAAGATAACTGGTGCTCATTTTCATCATTATCC700.2897830766683226No Hit
GTGTTATTTCAAAGCCCCCAAGGGAAACCGTTGGCTGTACAGACATTTTCA700.2897830766683226No Hit
CGTCATCATCTTCATCAGCAGCAAGTTTTACTTTTTTCTGTGGAACCTTGC690.2856433184302037No Hit
GGCAAATCAGAAAAGGGGATTGAGATTAAAATAGAAGACTTCAGTCTGGAT670.2773638019539659No Hit
TATATTTTTGTCTCCCCACCATTTCCATGTCTGACCACCGCTACTACTATG640.2649445272396092No Hit
GCGATTTAAAACATTGTCAGGTGAGGCAAATGCACAAGTAATAGAAAGCAA640.2649445272396092No Hit
CTGTTTCTTGAAGGATTCTTGTCCTTTTGATCTTGGTGTTGATGATGGTTT630.26080476900149036No Hit
TTCATCATCATCGTCATCATCTTCATCAGCAGCAAGTTTTACTTTTTTCTG600.24838549428713363No Hit
GGTGAGGCAAATGCACAAGTAATAGAAAGCAAAGGGCAAGGTTCACTGAAT590.24424573604901476No Hit
CTCATCCTTTGCACCAGCCCCTAAACTGACCGTTCTTAAAGATAACTGGTG580.24010597781089585No Hit
GCCAGATATCAACTGTTACAGAAATGAAATAAGACGGAAAATTTTTTAACA580.24010597781089585No Hit
CCCCAAGGGAAACCGTTGGCTGTACAGACATTTTCAAAGTTGCCAGTGTTA530.2194071866203014No Hit
GGAAAATTTTTTAACAAATTGTTTAAACTATTTTCTTAAAGAGACTTCCTC530.2194071866203014No Hit
CCTCCTCCTCTTCATCTTCTGACTCTGCATCTTCCTCCACAGCTACTAAGT530.2194071866203014No Hit
ACCGTTCTTAAAGATAACTGGTGCTCATTTTCATCATTATCCACCTTAAAG530.2194071866203014No Hit
GTCTCCCCACCATTTCCATGTCTGACCACCGCTACTACTATGTCCTATCAT520.2152674283821825No Hit
CTTGAAGGATTCTTGTCCTTTTGATCTTGGTGTTGATGATGGTTTTGAGTC510.2111276701440636No Hit
CCCAGGACTGTTCAATGCGCTTTTTCTATACTTGCTTGCATTTTTGCTTTA510.2111276701440636No Hit
CACCTTAAAGTGATAATCTTTGTCGGCCTTTAGTTCACAACCGAAAAGATA510.2111276701440636No Hit
CCTCCTCTTCATCTTCTGACTCTGCATCTTCCTCCACAGCTACTAAGTGCT510.2111276701440636No Hit
TCTTCATCTTCTGACTCTGCATCTTCCTCCACAGCTACTAAGTGCTGTCCA510.2111276701440636No Hit
CTCTTCATCTTCTGACTCTGCATCTTCCTCCACAGCTACTAAGTGCTGTCC500.2069879119059447No Hit
CTGGTGCTCATTTTCATCATTATCCACCTTAAAGTGATAATCTTTGTCGGC490.20284815366782583No Hit
GGTGTTTTAGGAGTTTTTTCCTGTTTCTTGAAGGATTCTTGTCCTTTTGAT480.19870839542970692No Hit
CAGCTAAGGAGATACAAAGGCGATTTAAAACATTGTCAGGTGAGGCAAATG460.1904288789534691No Hit
TATTTTTGTCTCCCCACCATTTCCATGTCTGACCACCGCTACTACTATGTC450.18628912071535023No Hit
GTCGATTTAAAACATTGTCAGGTGAGGCAAATGCACAAGTAATAGAAAGCA430.17800960423911244No Hit
CCCCATCTTTGGGCTTAATTTACATGTAGTGCCCAGGACTGTTCAATGCGC430.17800960423911244No Hit
CTCTTGGTCAGTCATCCGGAAGCAATTCTTCACATAATTGATGAATTTGGC430.17800960423911244No Hit
CTCTCCTTTTCCTTTCTCCCCATCTTTGGGCTTAATTTACATGTAGTGCCC420.17386984600099353No Hit
TCCACCTTAAAGTGATAATCTTTGTCGGCCTTTAGTTCACAACCGAAAAGA420.17386984600099353No Hit
TCCTCCTCTTCATCTTCTGACTCTGCATCTTCCTCCACAGCTACTAAGTGC410.16973008776287465No Hit
CTGGCGCTTTTTCTTCAGCTTCCTCATCATCAAAATCATCATCATCATCAT410.16973008776287465No Hit
GCGCTTTTTCTATACTTGCTTGCATTTTTGCTTTAATGTCTTCTACAGAAC400.16559032952475575No Hit
CGGAAAATTTTTTAACAAATTGTTTAAACTATTTTCTTAAAGAGACTTCCT390.16145057128663687No Hit
CTCCTTTTCCTTTCTCCCCATCTTTGGGCTTAATTTACATGTAGTGCCCAG380.15731081304851796No Hit
ATCATCTTCATCATCATCCTCTTCATCATCATCGTCATCATCTTCATCAGC370.15317105481039908No Hit
GACTGCCTTCGTAATTCATTGCCTCTGCTTCAACAATGTGCAACTCATCCT350.1448915383341613No Hit
CGCCCCACCATTTCCATGTCTGACCACCGCTACTACTATGTCCTATCATAA340.1407517800960424No Hit
CAGGACTGTTCAATGCGCTTTTTCTATACTTGCTTGCATTTTTGCTTTAAT340.1407517800960424No Hit
GTGCAACTCATCCTTTGCACCAGCCCCTAAACTGACCGTTCTTAAAGATAA330.1366120218579235No Hit
TGGCGCTTTTTCTTCAGCTTCCTCATCATCAAAATCATCATCATCATCATC330.1366120218579235No Hit
AGCAGCTAAGGAGATACAAAGGCGATTTAAAACATTGTCAGGTGAGGCAAA330.1366120218579235No Hit
AGGTGAGGCAAATGCACAAGTAATAGAAAGCAAAGGGCAAGGTTCACTGAA320.1324722636198046No Hit
GTGGTGTTATTTCAAAGCCCCCAAGGGAAACCGTTGGCTGTACAGACATTT320.1324722636198046No Hit
CAGGTGAGGCAAATGCACAAGTAATAGAAAGCAAAGGGCAAGGTTCACTGA310.1283325053816857No Hit
GCCCAGGACTGTTCAATGCGCTTTTTCTATACTTGCTTGCATTTTTGCTTT310.1283325053816857No Hit
CTAAACTGACCGTTCTTAAAGATAACTGGTGCTCATTTTCATCATTATCCA310.1283325053816857No Hit
TTCATCTTCTGACTCTGCATCTTCCTCCACAGCTACTAAGTGCTGTCCACT310.1283325053816857No Hit
TCATCATCAAAATCATCATCATCATCATCTTCATCATCATCCTCTTCATCA300.12419274714356682No Hit
TTCTACAGAACTAGGTCCTTTTGGTGTTTTAGGAGTTTTTTCCTGTTTCTT290.12005298890544792No Hit
GTGCTCATTTTCATCATTATCCACCTTAAAGTGATAATCTTTGTCGGCCTT280.11591323066732903No Hit
CATTTCCATGTCTGACCACCGCTACTACTATGTCCTATCATAACATTCCAT280.11591323066732903No Hit
GGAGTTTTTTCCTGTTTCTTGAAGGATTCTTGTCCTTTTGATCTTGGTGTT280.11591323066732903No Hit
CAGTCATCCGGAAGCAATTCTTCACATAATTGATGAATTTGGCTTCCACTT280.11591323066732903No Hit
CCATCTTTGGGCTTAATTTACATGTAGTGCCCAGGACTGTTCAATGCGCTT280.11591323066732903No Hit
TTCTTGAAGGATTCTTGTCCTTTTGATCTTGGTGTTGATGATGGTTTTGAG280.11591323066732903No Hit
GATGCATTCAAATATTTATTGAGCAGCTAAGGAGATACAAAGGCGATTTAA270.11177347242921014No Hit
GCAACTCATCCTTTGCACCAGCCCCTAAACTGACCGTTCTTAAAGATAACT270.11177347242921014No Hit
ACCTTAAAGTGATAATCTTTGTCGGCCTTTAGTTCACAACCGAAAAGATAG270.11177347242921014No Hit
TGCTTCAACAATGTGCAACTCATCCTTTGCACCAGCCCCTAAACTGACCGT260.10763371419109125No Hit
GCATTTTTGGCTGGAGTATCTCGTATAGATTTCTTCACTGGCGCTTTTTCT260.10763371419109125No Hit
CTTCCACTTTGGGAAGAGAACCACCTTTTTCTATACTTGCTTGCATTTTTG260.10763371419109125No Hit
GTCCACCTTAAAGTGATAATCTTTGTCGGCCTTTAGTTCACAACCGAAAAG260.10763371419109125No Hit
GCTTCAACAATGTGCAACTCATCCTTTGCACCAGCCCCTAAACTGACCGTT250.10349395595297235No Hit
CCATTTCCATGTCTGACCACCGCTACTACTATGTCCTATCATAACATTCCA250.10349395595297235No Hit
CTTAATTCATTGCCTCTGCTTCAACAATGTGCAACTCATCCTTTGCACCAG250.10349395595297235No Hit
GAGCAGCTAAGGAGATACAAAGGCGATTTAAAACATTGTCAGGTGAGGCAA250.10349395595297235No Hit
ATGTATATTTTTGTCTCCCCACCATTTCCATGTCTGACCACCGCTACTACT250.10349395595297235No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTGC206.84199E-445.0726171
CACATGT800.042.2555771
TCTACAG657.2759576E-1238.1383673
TTCTACA657.2759576E-1238.1383672
CTTCTAC657.2759576E-1238.1383671
GTGTTAC301.0438738E-437.87482545
CCTTGCT301.0438738E-437.87482545
ACATTCC250.001967725336.35983345
GATAGTT250.001967725336.35983345
ACTGCCT250.002050235436.058093
GTGCATT250.002050235436.058091
GACTGCC250.002050235436.058092
ATGTATA1000.033.8044624
CATGTAT1000.033.8044623
CTCCTCC707.185008E-1032.1947251
AGTGTTA352.714001E-432.19472544
ACCTTGC352.714001E-432.19472544
CCAGTGT352.747382E-432.12806742
AACCTTG352.747382E-432.12806743
GCCAGTG352.747382E-432.12806741
Produced by FastQC (version 0.11.2)