FastQCFastQC Report
Вс 9 дек 2018
chr5.1_trimmomatic.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamechr5.1_trimmomatic.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24051
Sequences flagged as poor quality0
Sequence length50-51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCACCATTTCCATGTCTGACCACCGCTACTACTATGTCCTATCATAA10074.1869360941333No Hit
CCACCATTTCCATGTCTGACCACCGCTACTACTATGTCCTATCATAACATT9904.116252962454784No Hit
CTCTGCTTCAACAATGTGCAACTCATCCTTTGCACCAGCCCCTAAACTGAC8153.3886324892935846No Hit
CACATGTATATTTTTGTCTCCCCACCATTTCCATGTCTGACCACCGCTACT7403.0767951436530705No Hit
CCCACCATTTCCATGTCTGACCACCGCTACTACTATGTCCTATCATAACAT6012.4988565963993183No Hit
CACCATTTCCATGTCTGACCACCGCTACTACTATGTCCTATCATAACATTC6002.4946987651241113No Hit
CTCCTCTTCATCTTCTGACTCTGCATCTTCCTCCACAGCTACTAAGTGCTG5962.478067440023284No Hit
CTTCGTAATTCATTGCCTCTGCTTCAACAATGTGCAACTCATCCTTTGCAC5872.4406469585464223No Hit
CTTCTACAGAACTAGGTCCTTTTGGTGTTTTAGGAGTTTTTTCCTGTTTCT5002.078915637603426No Hit
CTCCTCCTCTTCATCTTCTGACTCTGCATCTTCCTCCACAGCTACTAAGTG4922.0456529874017715No Hit
GTTTTAGGAGTTTTTTCCTGTTTCTTGAAGGATTCTTGTCCTTTTGATCTT4421.8377614236414288No Hit
CCCCACCATTTCCATGTCTGACCACCGCTACTACTATGTCCTATCATAACA4061.6880794977339821No Hit
CTTCATCATCATCGTCATCATCTTCATCAGCAGCAAGTTTTACTTTTTTCT3611.5009770903496735No Hit
GTCCTTTTGGTGTTTTAGGAGTTTTTTCCTGTTTCTTGAAGGATTCTTGTC3541.4718722714232257No Hit
GGCGATTTAAAACATTGTCAGGTGAGGCAAATGCACAAGTAATAGAAAGCA3251.3512951644422269No Hit
ATTTTTGTCTCCCCACCATTTCCATGTCTGACCACCGCTACTACTATGTCC3131.3014011891397448No Hit
CTGCCTTCGTAATTCATTGCCTCTGCTTCAACAATGTGCAACTCATCCTTT3041.263980707662883No Hit
GTATATTTTTGTCTCCCCACCATTTCCATGTCTGACCACCGCTACTACTAT3011.2515072138372625No Hit
GTCCTTTTGATCTTGGTGTTGATGATGGTTTTGAGTCTTTTCCATTCTGAT2981.239033720011642No Hit
CCTGTTTCTTGAAGGATTCTTGTCCTTTTGATCTTGGTGTTGATGATGGTT2280.9479855307471623No Hit
GTCATCATCTTCATCAGCAGCAAGTTTTACTTTTTTCTGTGGAACCTTGCT2270.9438276994719554No Hit
CCCAAGGGAAACCGTTGGCTGTACAGACATTTTCAAAGTTGCCAGTGTTAC2170.902249386719887No Hit
CTTAAAGTGATAATCTTTGTCGGCCTTTAGTTCACAACCGAAAAGATAGTT2010.8357240863165774No Hit
CTACAGAACTAGGTCCTTTTGGTGTTTTAGGAGTTTTTTCCTGTTTCTTGA1880.7816722797388882No Hit
GTTTCTTGAAGGATTCTTGTCCTTTTGATCTTGGTGTTGATGATGGTTTTG1860.7733566171884745No Hit
GCCTCTGCTTCAACAATGTGCAACTCATCCTTTGCACCAGCCCCTAAACTG1830.7608831233628539No Hit
ATATTTTTGTCTCCCCACCATTTCCATGTCTGACCACCGCTACTACTATGT1730.7193048106107854No Hit
GGACTGCCTTCGTAATTCATTGCCTCTGCTTCAACAATGTGCAACTCATCC1720.7151469793355786No Hit
GTGTTTTAGGAGTTTTTTCCTGTTTCTTGAAGGATTCTTGTCCTTTTGATC1700.7068313167851649No Hit
GTGCATTTTTGGCTGGAGTATCTCGTATAGATTTCTTCACTGGCGCTTTTT1670.6943578229595443No Hit
ACCATTTCCATGTCTGACCACCGCTACTACTATGTCCTATCATAACATTCC1660.6901999916843374No Hit
CTCATTTTCATCATTATCCACCTTAAAGTGATAATCTTTGTCGGCCTTTAG1630.6777264978587169No Hit
CGTAATTCATTGCCTCTGCTTCAACAATGTGCAACTCATCCTTTGCACCAG1570.6527795102074758No Hit
CATGTATATTTTTGTCTCCCCACCATTTCCATGTCTGACCACCGCTACTAC1400.5820963785289593No Hit
GGCGCTTTTTCTTCAGCTTCCTCATCATCAAAATCATCATCATCATCATCT1380.5737807159785456No Hit
CCACCTTAAAGTGATAATCTTTGTCGGCCTTTAGTTCACAACCGAAAAGAT1350.561307222152925No Hit
CCGTTCTTAAAGATAACTGGTGCTCATTTTCATCATTATCCACCTTAAAGT1320.5488337283273045No Hit
TTCGTAATTCATTGCCTCTGCTTCAACAATGTGCAACTCATCCTTTGCACC1290.5363602345016839No Hit
CTCGTATAGATTTCTTCACTGGCGCTTTTTCTTCAGCTTCCTCATCATCAA1250.5197289094008565No Hit
TCGTAATTCATTGCCTCTGCTTCAACAATGTGCAACTCATCCTTTGCACCA1240.5155710781256497No Hit
GCCTTCGTAATTCATTGCCTCTGCTTCAACAATGTGCAACTCATCCTTTGC1220.507255415575236No Hit
CCTTAAAGTGATAATCTTTGTCGGCCTTTAGTTCACAACCGAAAAGATAGT1220.507255415575236No Hit
TACAGAACTAGGTCCTTTTGGTGTTTTAGGAGTTTTTTCCTGTTTCTTGAA1180.4906240904744085No Hit
GTAATTCATTGCCTCTGCTTCAACAATGTGCAACTCATCCTTTGCACCAGC1170.48646625919920167No Hit
GCTCATTTTCATCATTATCCACCTTAAAGTGATAATCTTTGTCGGCCTTTA1010.41994095879589205No Hit
GTCAGGTGAGGCAAATGCACAAGTAATAGAAAGCAAAGGGCAAGGTTCACT990.41162529624547833No Hit
CTTCATCTTCTGACTCTGCATCTTCCTCCACAGCTACTAAGTGCTGTCCAC990.41162529624547833No Hit
ATCGTCATCATCTTCATCAGCAGCAAGTTTTACTTTTTTCTGTGGAACCTT950.39499397114465096No Hit
GACCGTTCTTAAAGATAACTGGTGCTCATTTTCATCATTATCCACCTTAAA940.3908361398694441No Hit
GCGCTTTTTCTTCAGCTTCCTCATCATCAAAATCATCATCATCATCATCTT900.3742048147686167No Hit
TTTAGGAGTTTTTTCCTGTTTCTTGAAGGATTCTTGTCCTTTTGATCTTGG900.3742048147686167No Hit
TCTTCATCATCATCGTCATCATCTTCATCAGCAGCAAGTTTTACTTTTTTC890.37004698349340986No Hit
GTGAGGCAAATGCACAAGTAATAGAAAGCAAAGGGCAAGGTTCACTGAATC860.3575734896677893No Hit
TGCCTTCGTAATTCATTGCCTCTGCTTCAACAATGTGCAACTCATCCTTTG860.3575734896677893No Hit
GTCAGTCATCCGGAAGCAATTCTTCACATAATTGATGAATTTGGCTTCCAC830.3450999958421687No Hit
CTTTTGATCTTGGTGTTGATGATGGTTTTGAGTCTTTTCCATTCTGATTTG820.34094216456696186No Hit
TGTTTTAGGAGTTTTTTCCTGTTTCTTGAAGGATTCTTGTCCTTTTGATCT780.3243108394661345No Hit
ATCCACCTTAAAGTGATAATCTTTGTCGGCCTTTAGTTCACAACCGAAAAG770.3201530081909276No Hit
CCGCCATTTCCATGTCTGACCACCGCTACTACTATGTCCTATCATAACATT750.3118373456405139No Hit
CTTCAACAATGTGCAACTCATCCTTTGCACCAGCCCCTAAACTGACCGTTC740.30767951436530705No Hit
CCCATCTTTGGGCTTAATTTACATGTAGTGCCCAGGACTGTTCAATGCGCT730.3035216830901002No Hit
TGTTTCTTGAAGGATTCTTGTCCTTTTGATCTTGGTGTTGATGATGGTTTT710.29520602053968653No Hit
CCTAAACTGACCGTTCTTAAAGATAACTGGTGCTCATTTTCATCATTATCC700.2910481892644797No Hit
GTGTTATTTCAAAGCCCCCAAGGGAAACCGTTGGCTGTACAGACATTTTCA700.2910481892644797No Hit
CGTCATCATCTTCATCAGCAGCAAGTTTTACTTTTTTCTGTGGAACCTTGC690.2868903579892728No Hit
GGCAAATCAGAAAAGGGGATTGAGATTAAAATAGAAGACTTCAGTCTGGAT670.27857469543885904No Hit
TATATTTTTGTCTCCCCACCATTTCCATGTCTGACCACCGCTACTACTATG640.2661012016132385No Hit
GCGATTTAAAACATTGTCAGGTGAGGCAAATGCACAAGTAATAGAAAGCAA640.2661012016132385No Hit
CTGTTTCTTGAAGGATTCTTGTCCTTTTGATCTTGGTGTTGATGATGGTTT630.26194337033803167No Hit
TTCATCATCATCGTCATCATCTTCATCAGCAGCAAGTTTTACTTTTTTCTG600.24946987651241112No Hit
GGTGAGGCAAATGCACAAGTAATAGAAAGCAAAGGGCAAGGTTCACTGAAT590.24531204523720426No Hit
CTCATCCTTTGCACCAGCCCCTAAACTGACCGTTCTTAAAGATAACTGGTG580.2411542139619974No Hit
GCCAGATATCAACTGTTACAGAAATGAAATAAGACGGAAAATTTTTTAACA580.2411542139619974No Hit
CCCCAAGGGAAACCGTTGGCTGTACAGACATTTTCAAAGTTGCCAGTGTTA530.22036505758596317No Hit
GGAAAATTTTTTAACAAATTGTTTAAACTATTTTCTTAAAGAGACTTCCTC530.22036505758596317No Hit
CCTCCTCCTCTTCATCTTCTGACTCTGCATCTTCCTCCACAGCTACTAAGT530.22036505758596317No Hit
ACCGTTCTTAAAGATAACTGGTGCTCATTTTCATCATTATCCACCTTAAAG530.22036505758596317No Hit
GTCTCCCCACCATTTCCATGTCTGACCACCGCTACTACTATGTCCTATCAT520.2162072263107563No Hit
CTTGAAGGATTCTTGTCCTTTTGATCTTGGTGTTGATGATGGTTTTGAGTC510.21204939503554945No Hit
CCCAGGACTGTTCAATGCGCTTTTTCTATACTTGCTTGCATTTTTGCTTTA510.21204939503554945No Hit
CACCTTAAAGTGATAATCTTTGTCGGCCTTTAGTTCACAACCGAAAAGATA510.21204939503554945No Hit
CCTCCTCTTCATCTTCTGACTCTGCATCTTCCTCCACAGCTACTAAGTGCT510.21204939503554945No Hit
TCTTCATCTTCTGACTCTGCATCTTCCTCCACAGCTACTAAGTGCTGTCCA510.21204939503554945No Hit
CTCTTCATCTTCTGACTCTGCATCTTCCTCCACAGCTACTAAGTGCTGTCC500.2078915637603426No Hit
CTGGTGCTCATTTTCATCATTATCCACCTTAAAGTGATAATCTTTGTCGGC490.20373373248513574No Hit
GGTGTTTTAGGAGTTTTTTCCTGTTTCTTGAAGGATTCTTGTCCTTTTGAT480.1995759012099289No Hit
CAGCTAAGGAGATACAAAGGCGATTTAAAACATTGTCAGGTGAGGCAAATG460.1912602386595152No Hit
TATTTTTGTCTCCCCACCATTTCCATGTCTGACCACCGCTACTACTATGTC450.18710240738430836No Hit
GTCGATTTAAAACATTGTCAGGTGAGGCAAATGCACAAGTAATAGAAAGCA430.17878674483389465No Hit
CCCCATCTTTGGGCTTAATTTACATGTAGTGCCCAGGACTGTTCAATGCGC430.17878674483389465No Hit
CTCTTGGTCAGTCATCCGGAAGCAATTCTTCACATAATTGATGAATTTGGC430.17878674483389465No Hit
CTCTCCTTTTCCTTTCTCCCCATCTTTGGGCTTAATTTACATGTAGTGCCC420.1746289135586878No Hit
TCCACCTTAAAGTGATAATCTTTGTCGGCCTTTAGTTCACAACCGAAAAGA420.1746289135586878No Hit
TCCTCCTCTTCATCTTCTGACTCTGCATCTTCCTCCACAGCTACTAAGTGC410.17047108228348093No Hit
CTGGCGCTTTTTCTTCAGCTTCCTCATCATCAAAATCATCATCATCATCAT410.17047108228348093No Hit
GCGCTTTTTCTATACTTGCTTGCATTTTTGCTTTAATGTCTTCTACAGAAC400.1663132510082741No Hit
CGGAAAATTTTTTAACAAATTGTTTAAACTATTTTCTTAAAGAGACTTCCT390.16215541973306724No Hit
CTCCTTTTCCTTTCTCCCCATCTTTGGGCTTAATTTACATGTAGTGCCCAG380.15799758845786038No Hit
ATCATCTTCATCATCATCCTCTTCATCATCATCGTCATCATCTTCATCAGC370.15383975718265352No Hit
GACTGCCTTCGTAATTCATTGCCTCTGCTTCAACAATGTGCAACTCATCCT350.14552409463223984No Hit
CGCCCCACCATTTCCATGTCTGACCACCGCTACTACTATGTCCTATCATAA340.14136626335703298No Hit
CAGGACTGTTCAATGCGCTTTTTCTATACTTGCTTGCATTTTTGCTTTAAT340.14136626335703298No Hit
GTGCAACTCATCCTTTGCACCAGCCCCTAAACTGACCGTTCTTAAAGATAA330.13720843208182612No Hit
TGGCGCTTTTTCTTCAGCTTCCTCATCATCAAAATCATCATCATCATCATC330.13720843208182612No Hit
AGCAGCTAAGGAGATACAAAGGCGATTTAAAACATTGTCAGGTGAGGCAAA330.13720843208182612No Hit
AGGTGAGGCAAATGCACAAGTAATAGAAAGCAAAGGGCAAGGTTCACTGAA320.13305060080661926No Hit
GTGGTGTTATTTCAAAGCCCCCAAGGGAAACCGTTGGCTGTACAGACATTT320.13305060080661926No Hit
CAGGTGAGGCAAATGCACAAGTAATAGAAAGCAAAGGGCAAGGTTCACTGA310.12889276953141243No Hit
GCCCAGGACTGTTCAATGCGCTTTTTCTATACTTGCTTGCATTTTTGCTTT310.12889276953141243No Hit
CTAAACTGACCGTTCTTAAAGATAACTGGTGCTCATTTTCATCATTATCCA310.12889276953141243No Hit
TTCATCTTCTGACTCTGCATCTTCCTCCACAGCTACTAAGTGCTGTCCACT310.12889276953141243No Hit
TCATCATCAAAATCATCATCATCATCATCTTCATCATCATCCTCTTCATCA300.12473493825620556No Hit
TTCTACAGAACTAGGTCCTTTTGGTGTTTTAGGAGTTTTTTCCTGTTTCTT290.1205771069809987No Hit
GTGCTCATTTTCATCATTATCCACCTTAAAGTGATAATCTTTGTCGGCCTT280.11641927570579186No Hit
CATTTCCATGTCTGACCACCGCTACTACTATGTCCTATCATAACATTCCAT280.11641927570579186No Hit
GGAGTTTTTTCCTGTTTCTTGAAGGATTCTTGTCCTTTTGATCTTGGTGTT280.11641927570579186No Hit
CAGTCATCCGGAAGCAATTCTTCACATAATTGATGAATTTGGCTTCCACTT280.11641927570579186No Hit
CCATCTTTGGGCTTAATTTACATGTAGTGCCCAGGACTGTTCAATGCGCTT280.11641927570579186No Hit
TTCTTGAAGGATTCTTGTCCTTTTGATCTTGGTGTTGATGATGGTTTTGAG280.11641927570579186No Hit
GATGCATTCAAATATTTATTGAGCAGCTAAGGAGATACAAAGGCGATTTAA270.112261444430585No Hit
GCAACTCATCCTTTGCACCAGCCCCTAAACTGACCGTTCTTAAAGATAACT270.112261444430585No Hit
ACCTTAAAGTGATAATCTTTGTCGGCCTTTAGTTCACAACCGAAAAGATAG270.112261444430585No Hit
TGCTTCAACAATGTGCAACTCATCCTTTGCACCAGCCCCTAAACTGACCGT260.10810361315537816No Hit
GCATTTTTGGCTGGAGTATCTCGTATAGATTTCTTCACTGGCGCTTTTTCT260.10810361315537816No Hit
CTTCCACTTTGGGAAGAGAACCACCTTTTTCTATACTTGCTTGCATTTTTG260.10810361315537816No Hit
GTCCACCTTAAAGTGATAATCTTTGTCGGCCTTTAGTTCACAACCGAAAAG260.10810361315537816No Hit
GCTTCAACAATGTGCAACTCATCCTTTGCACCAGCCCCTAAACTGACCGTT250.1039457818801713No Hit
CCATTTCCATGTCTGACCACCGCTACTACTATGTCCTATCATAACATTCCA250.1039457818801713No Hit
CTTAATTCATTGCCTCTGCTTCAACAATGTGCAACTCATCCTTTGCACCAG250.1039457818801713No Hit
GAGCAGCTAAGGAGATACAAAGGCGATTTAAAACATTGTCAGGTGAGGCAA250.1039457818801713No Hit
ATGTATATTTTTGTCTCCCCACCATTTCCATGTCTGACCACCGCTACTACT250.1039457818801713No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTGC206.899411E-444.995841
CACATGT800.042.1835981
CTTCTAC601.4006218E-1037.4965361
GTGTTAC250.00202535436.14697345
ACATTCC250.00202535436.14697345
GATAGTT250.00202535436.14697345
ACTGCCT250.00206737835.9966743
GACTGCC250.00206737835.9966742
CATGTAT950.035.5230333
TCTACAG701.8189894E-1135.3538783
TTCTGAT457.978524E-735.1428945
TTTTCCA458.244897E-734.99676538
CATTCTG458.244897E-734.99676543
GAGTCTT458.244897E-734.99676533
GTCTTTT458.244897E-734.99676535
AGTCTTT458.244897E-734.99676534
ATTCTGA458.244897E-734.99676544
TCTTTTC458.244897E-734.99676536
CTCCTCC653.3105607E-1034.6121861
TTCTACA653.3105607E-1034.6121862
Produced by FastQC (version 0.11.2)