FastQCFastQC Report
Чт 27 фев 2020
chr8.1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamechr8.1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17763
Sequences flagged as poor quality0
Sequence length25-51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAATGCCAGCACCGCGGGGCTGCTGCTCAGGACGCATTCCTGCCCCAG1180.6643022012047515No Hit
GTCCATTTTAGCATCTGGATATCTGTTTGTCCAGGTGTTCAGAAGTCTCTT940.5291898890953104No Hit
CTTCGTTTGATGAAAGGTTCTTTGTCTTCTTCAGATGCTTTAACACCACTT770.4334853346844565No Hit
CCCGTTTCTTTCATTGGTAACATCAGATTGATAAACTGGCGAGTTAGCCGA710.3997072566570962No Hit
GTTTGGATAATCTGGGCTCATGCTGGTCATTTCGAGCAGAAAATTTGTTGC680.3828182176434161No Hit
CTGGTATTTTTTTTTTCAATGCAAGTTCTCCATAGAATTTACTAAATAATT600.3377807802736024No Hit
GGGGTATAGCTCAGGCCCAGTTTGAAAGCCATCTGCAGTGCAGGAACGTAG530.29837302257501547No Hit
CTGGGAGTCATTATCTGTCTCACTTTCTGGATCTCTGTAATTATCATGAAT520.2927433429037888No Hit
CTCGGAGTTCTACTGGTTTTAACTTTGGCATCATCTTATAAATTATGTCCA490.2758543038901086No Hit
CCTTGTTGTTCGAATCCACATTTCTGATGATTCCATAAAACTGCTCCATAA470.2645949445476552No Hit
CTCTGGGCCACGGCCTGTAACGGGGTGATGAGGCTGCTGTGCTTGATCTGA470.2645949445476552No Hit
GGGCTCTCTTACTGCTGATGTCATCTGGGTCTTAAGTTCACCCAGAAAAGC470.2645949445476552No Hit
CTCCGGTTGAGAAATCAAATTGACTCATTTCCTCACTCAGGGTACTGTCTG460.2589652648764285No Hit
GGGGTTTTTGCTAGTTCAGCTCTTACATCATTGCTCCAATCCTTAAAGAGC450.25333558520520183No Hit
CCCTGATGCACCACAGTACTAATGCAATTCCTGAGAACTGGCCCTTTTGCT440.24770590553397512No Hit
CTCCCTTGAAGTCTGGGGATCTTCTTCCATGGACTTAGTGAAGTTGCACAG440.24770590553397512No Hit
CTGGGTCTTCAGGAGAGTGTTTCAGACTCTTTGGACTAACTCCCTCGAAAT430.2420762258627484No Hit
GGGGGTATAGCTCAGGCCCAGTTTGAAAGCCATCTGCAGTGCAGGAACGTA420.2364465461915217No Hit
GTCGCAAGTTTCAGTCCTTGGTGTTTCTGGTTTGCTCGCTCCCTGAAGCTC420.2364465461915217No Hit
GTTTGATGAAAGGTTCTTTGTCTTCTTCAGATGCTTTAACACCACTTTATT420.2364465461915217No Hit
CCCATCTTCTTGGTGATTTGAGTATCAAAGGTAGATTCATTTAGCTTTGTA410.23081686652029498No Hit
CCCGCGATCACCTTCTCTGAATCCTCTGAACTGGATCCAGAAAGCTCTAAG390.21955750717784156No Hit
CTCTGAACTCCGGTTGAGAAATCAAATTGACTCATTTCCTCACTCAGGGTA390.21955750717784156No Hit
CTGGCAATAAAGGAACGAATTTATAAAAGAGTTCAATGGATTTGTGTCGAC380.21392782750661488No Hit
CTTGAAGTCTGGGGATCTTCTTCCATGGACTTAGTGAAGTTGCACAGAAGT370.20829814783538814No Hit
CGCAAGTTTCAGTCCTTGGTGTTTCTGGTTTGCTCGCTCCCTGAAGCTCTG370.20829814783538814No Hit
GGTTTGGATAATCTGGGCTCATGCTGGTCATTTCGAGCAGAAAATTTGTTG370.20829814783538814No Hit
CCATCTTCTTGGTGATTTGAGTATCAAAGGTAGATTCATTTAGCTTTGTAA350.19703878849293474No Hit
GTCCAGGGTTCTCATCGATCAATCCTTGAATCAGCACATCTTTTGCCAACT340.19140910882170806No Hit
CTGATAAACACGGTCGTCACCAGTGTCTGTCTGGGTGAGGAACATCTGCTT340.19140910882170806No Hit
GCCCATCTTCTTGGTGATTTGAGTATCAAAGGTAGATTCATTTAGCTTTGT330.18577942915048135No Hit
ATTTTTTTTTTCAATGCAAGTTCTCCATAGAATTTACTAAATAATTCTCCA330.18577942915048135No Hit
CTCAATTAATCCTAACCTGGAGGTCATGGGCACAACGCTATAGGTCCTCAG320.18014974947925463No Hit
CTTGTTGTTCGAATCCACATTTCTGATGATTCCATAAAACTGCTCCATAAA320.18014974947925463No Hit
CCGCGGAGTTCCTGGAGGAAATTCCCTGGACTGCATGGGGAAGTGAGCAAC320.18014974947925463No Hit
GTTCCTATGCTGCTTCTACAATGTTACATCAACTATACTTAGCTTTACTCT310.17452006980802792No Hit
CATGGTTCAAAAAATTCTGCTTGTTTCTCAGGGAGAATCTCTCTGCAAAAT310.17452006980802792No Hit
CGCGGAGTTCCTGGAGGAAATTCCCTGGACTGCATGGGGAAGTGAGCAACG310.17452006980802792No Hit
GGGGTTCAGAAGTTCTCGGAGTTCTACTGGTTTTAACTTTGGCATCATCTT300.1688903901368012No Hit
GCTTGTTTCTCAGGGAGAATCTCTCTGCAAAATTCCACCAGGTTAATGAAA300.1688903901368012No Hit
CTTTCATCATCTCTATGTCTCATGACTTGAACGAAGTCCTTGCTCTTTAAC300.1688903901368012No Hit
CCCTGTTGGAGTGGCCAAGCCTGTCCATGAAAGAATAGTGGCCACTATCTC300.1688903901368012No Hit
CTTCAGGAGAGTGTTTCAGACTCTTTGGACTAACTCCCTCGAAATATTTTA290.1632607104655745No Hit
GCCACCTCTTTGCCAAATTTCACAAATAAAGCAAAGCAAGAATACTTTTCT290.1632607104655745No Hit
GTGAGGAACATCTGCTTGCAGCGCTGAATGAGCTCAACGTACATGAAGTCA290.1632607104655745No Hit
CTCCACACGACAAAGTACCACCTCCAGACAGAGTGTTTTCAACACTCCATG290.1632607104655745No Hit
GCCAGAACCAGAAGGGCATTCCAAGGCTTCCCCACATTTCCTCCATTTACG280.15763103079434782No Hit
CTCGAGGCAGGAACACATCCAGGAAAATGACAGGTTTCATCTCTCTAAAGG280.15763103079434782No Hit
GTCCCTCTGGGCCACGGCCTGTAACGGGGTGATGAGGCTGCTGTGCTTGAT280.15763103079434782No Hit
CTCGTTTTTGCTCAGCAACGCCTTTTCTGGCATACATCAAACTGAGCTTCT280.15763103079434782No Hit
CTCAGATGGGTCTCTAGGATATCTTTGTATGGGGACATCTTTAGAGCTTTC280.15763103079434782No Hit
GGCATCTGATAAACACGGTCGTCACCAGTGTCTGTCTGGGTGAGGAACATC280.15763103079434782No Hit
CTGGTTTTTGACTCTTTATGCAGCTCCTTCAGTAGTTTCATAGCAAGTGAG270.1520013511231211No Hit
GTCGACATTCTGTCTGGGGCCTCCCACAATGAGCTAAAAGCCACTTGACCA270.1520013511231211No Hit
GTAGATATTATTAAAGGCAAGTGATGCTCCCAGCCTCTTGAAAGCATTGGG270.1520013511231211No Hit
CTCTCTCAGATGGGTCTCTAGGATATCTTTGTATGGGGACATCTTTAGAGC260.1463716714518944No Hit
GGGTATAGCTCAGGCCCAGTTTGAAAGCCATCTGCAGTGCAGGAACGTAGG260.1463716714518944No Hit
GGAGGACTGGCGGGGGGGGGCAGGGACTGCACATGGGAAGGAGCCACTTTT260.1463716714518944No Hit
CCATGGTTCAAAAAATTCTGCTTGTTTCTCAGGGAGAATCTCTCTGCAAAA250.14074199178066768No Hit
CTTTAGTACAAAATTAAAAATCTCCCTTGAAGTCTGGGGATCTTCTTCCAT250.14074199178066768No Hit
GTGCGATCTTCTGGCCCATTTTTTCTAAGAAAATACATAAAAACTTTAGGA250.14074199178066768No Hit
TGTAGATATTATTAAAGGCAAGTGATGCTCCCAGCCTCTTGAAAGCATTGG250.14074199178066768No Hit
GTTCAGAAGTTCTCGGAGTTCTACTGGTTTTAACTTTGGCATCATCTTATA250.14074199178066768No Hit
GTCATAGGGAGGCAGGTCATTGGCCATCACGATGCCTAGCAATTGAATCCC240.13511231210944097No Hit
CTCGTTTTCTAAAAGACGTGACTGTTTCAGTACGATTAGCGCCCTTATACA240.13511231210944097No Hit
CTGGAGAGTGCCCTGGGAGGCCTGGGTCTCCACAAACATCGGAGTGAGAAC240.13511231210944097No Hit
GTTTCTTTCATTGGTAACATCAGATTGATAAACTGGCGAGTTAGCCGAAAA240.13511231210944097No Hit
CCCATTTTTTCTAAGAAAATACATAAAAACTTTAGGATTTCTTCTCTACAT240.13511231210944097No Hit
CTTTAAATGTAGATCCAGCTTTGTGTCAGCAAGTAGACTAATATATGTTGT230.12948263243821426No Hit
GCCCGTTTCTTTCATTGGTAACATCAGATTGATAAACTGGCGAGTTAGCCG230.12948263243821426No Hit
GCTCTGTCAACATCATCTTGCAGGAGGTAAAGCAGACTCAGCTCTTGACTG230.12948263243821426No Hit
GTCTGTAATGCCAGCACCGCGGGGCTGCTGCTCAGGACGCATTCCTGCCCC230.12948263243821426No Hit
CGGGTCTGGAGAGTGCCCTGGGAGGCCTGGGTCTCCACAAACATCGGAGTG230.12948263243821426No Hit
GTGCTTTCCAATGTGCCAGAACCAGAAGGGCATTCCAAGGCTTCCCCACAT230.12948263243821426No Hit
GGCTCTCTTACTGCTGATGTCATCTGGGTCTTAAGTTCACCCAGAAAAGCG230.12948263243821426No Hit
CTTGGCATCTGATAAACACGGTCGTCACCAGTGTCTGTCTGGGTGAGGAAC230.12948263243821426No Hit
CTCACCTAATCTTTGATGTTACTATAACCTTATCTTTGATTTAACCCATAC220.12385295276698756No Hit
GGCTCACACAGCGTCTGCACCAGGACTCTCATCAGGTGTGTATTACACAAG220.12385295276698756No Hit
GTATAGCTCAGGCCCAGTTTGAAAGCCATCTGCAGTGCAGGAACGTAGGCT220.12385295276698756No Hit
CTTGGTGTTTCTGGTTTGCTCGCTCCCTGAAGCTCTGGTCTAACATGCCGT210.11822327309576085No Hit
CTCTCAGATGGGTCTCTAGGATATCTTTGTATGGGGACATCTTTAGAGCTT210.11822327309576085No Hit
GTCAGAGCTCAGGAGATGTCTGAAGAGATCCACGTAGTCTTTGTATGTGGG210.11822327309576085No Hit
CTCCTCTCCAGCAGCTGATTCTCTCCTGCCATGTTCTCTGTAAATGCATCG210.11822327309576085No Hit
GCCATTTTAGTTTCGAGAGGGGAATCTTTGGCCCACCATGAATCACACTTC210.11822327309576085No Hit
CTTGCCACAAACTCCTTATTCTTATGACCAGTAGAAGTATCCTTGAAGGAA210.11822327309576085No Hit
CACGTAGTCTTTGTATGTGGGCACCTTCCATTTGCCAGTTCTGACTTCCCC210.11822327309576085No Hit
CCTACATGTTGTAGAAGGATGGGAAACGAATTCCACAACGGGGTTCAGAAG210.11822327309576085No Hit
CCGTTTCTTTCATTGGTAACATCAGATTGATAAACTGGCGAGTTAGCCGAA210.11822327309576085No Hit
CTTTGAGATAATTCCAATGCATCTAGAAACTTTTTCATGCAGTCCACATAA210.11822327309576085No Hit
GGCGCTTCAGGTCGATCAGATTTTCAAAAATAAGCAAGTTCTTTTCTGGTT200.11259359342453415No Hit
CGGGCACTGTCTATCATCTTCATTTTCATGGAAAACTTGCAACTCCTGATC200.11259359342453415No Hit
CTCCTTGTTGTTCGAATCCACATTTCTGATGATTCCATAAAACTGCTCCAT200.11259359342453415No Hit
GCTGGATGCAAGTTAGCCGCTGGATCTGAAGTTGGGATCATCCAAACACCA200.11259359342453415No Hit
CAGGGAAAGAAGTTCTGTGCCAACAGAATGATGCAAATCTGTGGACTGAGA200.11259359342453415No Hit
CCGCGATCACCTTCTCTGAATCCTCTGAACTGGATCCAGAAAGCTCTAAGA200.11259359342453415No Hit
GGCTGCATTACATGTCTGTCAATATAAATTGACCATTCTTCTAGAGCATTC200.11259359342453415No Hit
CTCATGACTTGAACGAAGTCCTTGCTCTTTAACTGGAAGTACAGCTCTGTC200.11259359342453415No Hit
GCCAGACTCTCCATGTATATCACCAAGGCTTCAAACACAAACTGTTCCACC190.10696391375330744No Hit
GGGGCTTTGAGTCTTTGTTCATTTTTAAAAGTAGCATGTTGGTAATGTCGT190.10696391375330744No Hit
CCGGGCACTGTCTATCATCTTCATTTTCATGGAAAACTTGCAACTCCTGAT190.10696391375330744No Hit
ATTTTTTCTAAGAAAATACATAAAAACTTTAGGATTTCTTCTCTACATTCA190.10696391375330744No Hit
GTCAGAAGATTTTTGTTTATTTGTCCTCCTAGAGATCCAAGCATTTGTACT190.10696391375330744No Hit
GTGGGCACACCACTTTAACAAGACTTCAAATAGAGACACGTAGTTGTCCAG190.10696391375330744No Hit
GTGGAGGACTGGCGGGGGGGGGCAGGGACTGCACATGGGAAGGAGCCACTT190.10696391375330744No Hit
GCATCTGATAAACACGGTCGTCACCAGTGTCTGTCTGGGTGAGGAACATCT190.10696391375330744No Hit
CTTGTTTCTCAGGGAGAATCTCTCTGCAAAATTCCACCAGGTTAATGAAAG190.10696391375330744No Hit
GCCTTGTTTGCTTATAAAGCTGATGAACTCCTGAATTTCTGTTAAAGCCTG180.10133423408208073No Hit
CCAGATTTTATTTAGGTCTGGGGGGTTCTCACTGTCTATACTGGCTGTAGA180.10133423408208073No Hit
GTGGGAAACTGTCTATCTGCATCACCACGAGGTGCTCCAGAACTGGAGTAT180.10133423408208073No Hit
CGGGCTCACACAGCGTCTGCACCAGGACTCTCATCAGGTGTGTATTACACA180.10133423408208073No Hit
CCTTGAAGTCTGGGGATCTTCTTCCATGGACTTAGTGAAGTTGCACAGAAG180.10133423408208073No Hit
CTCTCTTACTGCTGATGTCATCTGGGTCTTAAGTTCACCCAGAAAAGCGCG180.10133423408208073No Hit
CTAGAATCTTATAGTAGCCCATCTTCTTGGTGATTTGAGTATCAAAGGTAG180.10133423408208073No Hit
CTAGAAACTTTTTCATGCAGTCCACATAATTATTGAACCGCGGAGTTCCTG180.10133423408208073No Hit
CATCTTCTTGGTGATTTGAGTATCAAAGGTAGATTCATTTAGCTTTGTAAA180.10133423408208073No Hit
TGTCAATATAAATTGACCATTCTTCTAGAGCATTCAGGCCTACTTCTGCCA180.10133423408208073No Hit
CCCACATTTCCTCCATTTACGGAGACAGCTGTTTCACTAACTTGCAGCACT180.10133423408208073No Hit
GTCTGGGTGAGGAACATCTGCTTGCAGCGCTGAATGAGCTCAACGTACATG180.10133423408208073No Hit
GTCCGCTTATAGAGCTGGTACATGGGTGGGGCTCCCTGTCCCCCTTCTGGC180.10133423408208073No Hit
CTCCTTCAGTAGTTTCATAGCAAGTGAGAAATTGTTCTGCTTCCGGGCACT180.10133423408208073No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCACC250.002059411535.97295810
Produced by FastQC (version 0.11.2)