PMID- 6286143
OWN - NLM
STAT- MEDLINE
DA  - 19821021
DCOM- 19821021
LR  - 20061115
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 29
IP  - 1
DP  - 1982 May
TI  - Cloning yeast telomeres on linear plasmid vectors.
PG  - 245-55
AB  - We have constructed a linear yeast plasmid by joining fragments from the termini 
      of Tetrahymena ribosomal DNA to a yeast vector. Structural features of the
      terminus region of the Tetrahymena rDNA plasmid maintained in the yeast linear
      plasmid include a set of specifically placed single-strand interruptions within
      the cluster of hexanucleotide (C4A2) repeat units. An artificially constructed
      hairpin terminus was unable to stabilize a linear plasmid in yeast. The fact that
      yeast can recognize and use DNA ends from the distantly related organism
      Tetrahymena suggests that the structural features required for telomere
      replication and resolution have been highly conserved in evolution. The linear
      plasmid was used as a vector to clone chromosomal telomeres from yeast. One
      Tetrahymena end was removed by restriction digestion, and yeast fragments that
      could function as an end on a linear plasmid were selected. Restriction mapping
      and hybridization analysis demonstrated that these fragments were yeast
      telomeres, and suggested that all yeast chromosomes might have a common telomere 
      sequence. Yeast telomeres appear to be similar in structure to the rDNA of
      Tetrahymena, in which specific nicks or gaps are present within a simple repeated
      sequence near the terminus of the DNA.
FAU - Szostak, J W
AU  - Szostak JW
FAU - Blackburn, E H
AU  - Blackburn EH
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - UNITED STATES
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (DNA, Fungal)
RN  - EC 3.1.21.- (DNA Restriction Enzymes)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Chromosomes/*metabolism
MH  - *Cloning, Molecular
MH  - *DNA Replication
MH  - DNA Restriction Enzymes
MH  - DNA, Fungal
MH  - Genetic Vectors
MH  - Nucleic Acid Conformation
MH  - Nucleic Acid Hybridization
MH  - *Plasmids
MH  - Repetitive Sequences, Nucleic Acid
MH  - Saccharomyces cerevisiae/*genetics
MH  - Tetrahymena
EDAT- 1982/05/01
MHDA- 1982/05/01 00:01
CRDT- 1982/05/01 00:00
AID - 0092-8674(82)90109-X [pii]
PST - ppublish
SO  - Cell. 1982 May;29(1):245-55.