REM -------------------------------------------------------------------- 1SVY REM 1SVY REM STRIDE: Knowledge-based secondary structure assignment 1SVY REM Please cite: D.Frishman & P.Argos, Proteins XX, XXX-XXX, 1995 1SVY REM 1SVY REM Residue accessible surface area calculation 1SVY REM Please cite: F.Eisenhaber & P.Argos, J.Comp.Chem. 14, 1272-1280, 1993 1SVY REM F.Eisenhaber et al., J.Comp.Chem., 1994, submitted 1SVY REM 1SVY REM ------------------------ General information ----------------------- 1SVY REM 1SVY HDR ACTIN-BINDING PROTEIN 10-AUG-98 1SVY 1SVY CMP MOL_ID: 1; 1SVY CMP MOLECULE: SEVERIN; 1SVY CMP CHAIN: A; 1SVY CMP FRAGMENT: DOMAIN 2; 1SVY CMP ENGINEERED: YES 1SVY SRC MOL_ID: 1; 1SVY SRC ORGANISM_SCIENTIFIC: DICTYOSTELIUM DISCOIDEUM; 1SVY SRC ORGANISM_COMMON: SLIME MOLD; 1SVY SRC EXPRESSION_SYSTEM: ESCHERICHIA COLI 1SVY AUT Y.A.PUIUS,E.V.FEDOROV,L.EICHINGER,M.SULLIVAN,M.SCHLEICHER, 1SVY AUT S.C.ALMO 1SVY REM 1SVY REM -------------------- Secondary structure summary ------------------- 1SVY REM 1SVY CHN /webclu/data/stride/pdb/pdb1svy.ent A 1SVY REM 1SVY REM . . . . . 1SVY SEQ 1 EYKPRLLHISGDKNAKVAEVPLATSSLNSGDCFLLDAGLTIYQFNGSKSS 50 1SVY STR EEEEEETTTTTEEEEE GGG BTTTEEEEETTTEEEEEETTTT 1SVY REM 1SVY REM . . . . . 1SVY SEQ 51 PQEKNKAAEVARAIDAERKGLPKVEVFETDSDIPAEFWKLLGGKGAIAAK 100 1SVY STR HHHHHHHHHHHHHHHHHHTTT EEEEETTTTTT HHHHHHH B 1SVY REM 1SVY REM 1SVY SEQ 101 H 101 1SVY STR 1SVY REM 1SVY REM 1SVY REM 1SVY LOC AlphaHelix PRO 208 A ARG 225 A 1SVY LOC AlphaHelix ALA 243 A LEU 249 A 1SVY LOC 310Helix THR 181 A SER 183 A 1SVY LOC Strand ARG 162 A SER 167 A 1SVY LOC Strand LYS 173 A VAL 177 A 1SVY LOC Strand CYS 189 A ASP 193 A 1SVY LOC Strand THR 197 A ASN 202 A 1SVY LOC Strand LYS 230 A PHE 234 A 1SVY LOC TurnII' GLY 168 A ASN 171 A 1SVY LOC TurnIV ASP 169 A ALA 172 A 1SVY LOC TurnI ASN 185 A ASP 188 A 1SVY LOC TurnIV ALA 194 A THR 197 A 1SVY LOC TurnVIII GLY 195 A ILE 198 A 1SVY LOC TurnI GLY 203 A SER 206 A 1SVY LOC TurnI' ARG 225 A LEU 228 A 1SVY LOC TurnIV PHE 234 A ASP 238 A 1SVY LOC TurnIV ASP 238 A ILE 241 A 1SVY REM 1SVY REM --------------- Detailed secondary structure assignment------------- 1SVY REM 1SVY REM |---Residue---| |--Structure--| |-Phi-| |-Psi-| |-Area-| 1SVY ASG GLU A 158 1 C Coil 360.00 137.37 166.4 1SVY ASG TYR A 159 2 C Coil -97.57 20.71 137.0 1SVY ASG LYS A 160 3 C Coil -86.01 119.08 157.5 1SVY ASG PRO A 161 4 C Coil -55.78 140.72 39.2 1SVY ASG ARG A 162 5 E Strand -142.75 156.28 80.6 1SVY ASG LEU A 163 6 E Strand -116.57 126.81 0.0 1SVY ASG LEU A 164 7 E Strand -108.56 140.15 12.6 1SVY ASG HIS A 165 8 E Strand -112.91 120.73 8.4 1SVY ASG ILE A 166 9 E Strand -105.92 113.92 0.0 1SVY ASG SER A 167 10 E Strand -135.47 179.41 40.5 1SVY ASG GLY A 168 11 T Turn 118.47 -7.04 36.2 1SVY ASG ASP A 169 12 T Turn 58.63 -105.09 92.9 1SVY ASG LYS A 170 13 T Turn -81.15 -14.13 143.0 1SVY ASG ASN A 171 14 T Turn -118.22 72.49 134.5 1SVY ASG ALA A 172 15 T Turn -61.05 132.38 19.6 1SVY ASG LYS A 173 16 E Strand -134.27 151.60 146.7 1SVY ASG VAL A 174 17 E Strand -122.29 142.62 53.4 1SVY ASG ALA A 175 18 E Strand -157.12 145.51 57.7 1SVY ASG GLU A 176 19 E Strand -69.28 144.38 124.1 1SVY ASG VAL A 177 20 E Strand -136.04 167.51 28.1 1SVY ASG PRO A 178 21 C Coil -59.41 134.69 97.0 1SVY ASG LEU A 179 22 C Coil -73.95 94.73 62.7 1SVY ASG ALA A 180 23 C Coil -164.21 151.14 24.9 1SVY ASG THR A 181 24 G 310Helix -60.41 -34.38 14.2 1SVY ASG SER A 182 25 G 310Helix -69.01 -4.91 109.9 1SVY ASG SER A 183 26 G 310Helix -87.79 -15.77 21.3 1SVY ASG LEU A 184 27 C Coil -78.70 160.25 2.9 1SVY ASG ASN A 185 28 B Bridge -155.20 150.40 2.0 1SVY ASG SER A 186 29 T Turn -68.47 -12.76 16.8 1SVY ASG GLY A 187 30 T Turn -94.99 -9.54 16.9 1SVY ASG ASP A 188 31 T Turn -119.29 -169.66 20.8 1SVY ASG CYS A 189 32 E Strand -122.97 151.89 0.0 1SVY ASG PHE A 190 33 E Strand -139.57 152.44 0.0 1SVY ASG LEU A 191 34 E Strand -123.71 114.34 0.0 1SVY ASG LEU A 192 35 E Strand -93.33 102.68 0.0 1SVY ASG ASP A 193 36 E Strand -88.59 103.56 0.4 1SVY ASG ALA A 194 37 T Turn -101.12 10.87 21.2 1SVY ASG GLY A 195 38 T Turn 99.77 -83.92 0.3 1SVY ASG LEU A 196 39 T Turn -84.39 -14.26 96.9 1SVY ASG THR A 197 40 E Strand -135.77 135.32 33.0 1SVY ASG ILE A 198 41 E Strand -117.59 122.08 0.0 1SVY ASG TYR A 199 42 E Strand -102.87 122.00 17.4 1SVY ASG GLN A 200 43 E Strand -104.81 117.16 8.6 1SVY ASG PHE A 201 44 E Strand -104.84 118.99 0.8 1SVY ASG ASN A 202 45 E Strand -98.38 114.22 27.7 1SVY ASG GLY A 203 46 T Turn -58.93 148.74 1.7 1SVY ASG SER A 204 47 T Turn -61.89 -28.27 50.3 1SVY ASG LYS A 205 48 T Turn -102.86 11.55 156.8 1SVY ASG SER A 206 49 T Turn -68.33 156.41 11.2 1SVY ASG SER A 207 50 C Coil -92.35 162.35 6.6 1SVY ASG PRO A 208 51 H AlphaHelix -60.34 -38.00 86.1 1SVY ASG GLN A 209 52 H AlphaHelix -62.90 -39.24 65.6 1SVY ASG GLU A 210 53 H AlphaHelix -67.96 -42.11 3.0 1SVY ASG LYS A 211 54 H AlphaHelix -62.35 -43.94 68.4 1SVY ASG ASN A 212 55 H AlphaHelix -63.02 -42.05 109.2 1SVY ASG LYS A 213 56 H AlphaHelix -63.41 -47.15 52.9 1SVY ASG ALA A 214 57 H AlphaHelix -57.15 -45.31 0.0 1SVY ASG ALA A 215 58 H AlphaHelix -62.90 -39.16 19.1 1SVY ASG GLU A 216 59 H AlphaHelix -64.80 -42.18 97.5 1SVY ASG VAL A 217 60 H AlphaHelix -66.42 -41.97 22.6 1SVY ASG ALA A 218 61 H AlphaHelix -60.34 -43.36 3.2 1SVY ASG ARG A 219 62 H AlphaHelix -65.83 -37.66 158.5 1SVY ASG ALA A 220 63 H AlphaHelix -65.74 -41.23 54.4 1SVY ASG ILE A 221 64 H AlphaHelix -64.86 -46.58 13.3 1SVY ASG ASP A 222 65 H AlphaHelix -57.61 -43.83 43.1 1SVY ASG ALA A 223 66 H AlphaHelix -61.62 -49.41 58.1 1SVY ASG GLU A 224 67 H AlphaHelix -57.95 -33.86 167.6 1SVY ASG ARG A 225 68 H AlphaHelix -92.66 27.47 46.3 1SVY ASG LYS A 226 69 T Turn 46.43 37.30 183.2 1SVY ASG GLY A 227 70 T Turn 76.71 0.30 42.8 1SVY ASG LEU A 228 71 T Turn -62.19 -41.69 106.7 1SVY ASG PRO A 229 72 C Coil -63.99 152.24 8.3 1SVY ASG LYS A 230 73 E Strand -100.63 138.52 146.5 1SVY ASG VAL A 231 74 E Strand -114.59 127.98 64.5 1SVY ASG GLU A 232 75 E Strand -116.14 134.47 88.8 1SVY ASG VAL A 233 76 E Strand -125.95 136.27 82.1 1SVY ASG PHE A 234 77 E Strand -155.12 360.00 13.6 1SVY ASG GLU A 236 78 T Turn 360.00 -36.68 45.4 1SVY ASG THR A 237 79 T Turn -88.79 3.30 116.6 1SVY ASG ASP A 238 80 T Turn -59.44 145.80 77.6 1SVY ASG SER A 239 81 T Turn -89.94 -7.37 128.6 1SVY ASG ASP A 240 82 T Turn -122.04 36.64 120.3 1SVY ASG ILE A 241 83 T Turn -66.80 132.29 23.4 1SVY ASG PRO A 242 84 C Coil -61.21 135.62 31.1 1SVY ASG ALA A 243 85 H AlphaHelix -56.73 -33.81 79.7 1SVY ASG GLU A 244 86 H AlphaHelix -61.40 -29.00 77.5 1SVY ASG PHE A 245 87 H AlphaHelix -62.22 -50.87 0.0 1SVY ASG TRP A 246 88 H AlphaHelix -66.13 -38.69 21.0 1SVY ASG LYS A 247 89 H AlphaHelix -58.62 -47.36 150.8 1SVY ASG LEU A 248 90 H AlphaHelix -65.07 -26.13 45.7 1SVY ASG LEU A 249 91 H AlphaHelix -85.30 1.73 6.5 1SVY ASG GLY A 250 92 C Coil 92.25 5.84 73.4 1SVY ASG GLY A 251 93 C Coil 123.74 140.91 32.4 1SVY ASG LYS A 252 94 C Coil -69.99 139.59 137.1 1SVY ASG GLY A 253 95 C Coil -164.17 178.70 33.9 1SVY ASG ALA A 254 96 C Coil -67.21 134.16 106.6 1SVY ASG ILE A 255 97 C Coil -92.40 138.33 33.8 1SVY ASG ALA A 256 98 B Bridge -66.18 145.19 52.0 1SVY ASG ALA A 257 99 C Coil -66.32 -30.38 53.5 1SVY ASG LYS A 258 100 C Coil -144.62 162.97 160.9 1SVY ASG HIS A 259 101 C Coil -80.63 360.00 167.0 1SVY