FastQCFastQC Report
Пн 26 дек 2016
SRR4240380_out.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4240380_out.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5119144
Sequences flagged as poor quality0
Sequence length1-36
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN152990.29885855916536047No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN125030.2442400526337997No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN101940.19913485535863026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTCG2550.013.5024699
TCGGAAG5950.013.0422813
CGGAAGA5250.011.3764364
AGGCTCG1150.002097182410.42255710
AGTGGCC1250.00405966079.62403522
CGGAAGG1400.00101369959.5988684
GGAAGAG6450.09.4969815
GATCGGA11300.09.3862821
GCCGTCT2201.0030259E-58.8772619
AGAGCTC4054.7293724E-118.4984078
TGCCGTC2352.3258783E-58.30387118
AGATCGG9750.07.728661530
ATCGGAA12750.07.13411862
CCGTCTT4656.3082553E-97.109753620
CGTATGC4151.7090952E-56.141131414
TCGTATG6901.4468242E-85.646456713
GAAGAGC5303.8475882E-65.64046766
GTATGCC4004.2226847E-45.62359215
TATGCCG4400.00150593825.114304516
GCTCGTA4706.633726E-45.101882511
Produced by FastQC (version 0.11.2)