FastQCFastQC Report
Пн 19 дек 2016
SRR.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4981466
Sequences flagged as poor quality0
Sequence length30-36
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA173060.3474077711260099Illumina Single End Adapter 1 (100% over 33bp)
GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGAT138010.27704695766266396Illumina Single End Adapter 1 (100% over 33bp)
GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTAGAT116710.2342884604652526Illumina Single End Adapter 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCTC87850.028.7113788
GAGCTCG88350.028.6831199
TCGGAAG93800.028.4480133
CGGAAGA92500.028.137184
GAAGAGC90700.028.0084236
GGAAGAG94150.027.6518845
GCTCGTA89050.027.44753511
GATCGGA97650.027.3602311
CGTATGC86950.027.35710514
AAGAGCT93100.027.2706157
AGCTCGT89950.027.2576510
GCCGTCT84650.027.08319719
ATCGGAA98950.026.9223162
TGCCGTC85150.026.91983218
ATGCCGT86250.026.76573617
GTATGCC88450.026.74005515
TATGCCG88000.026.68976416
TCGTATG91600.026.45344413
CTCGTAT92600.026.39395312
CCGTCTT87350.026.26897620
Produced by FastQC (version 0.11.2)