RID: ANC144SP01N Job Title:POL1_CPMVS P03600 RNA1 polyprotein (B RNA Program: BLASTP Database: swissprot Non-redundant UniProtKB/SwissProt sequences Query #1: POL1_CPMVS P03600 RNA1 polyprotein (B RNA polyprotein) (Bottom component polyprotein) (Genome polyprotein B) (P1) (Protease cofactor) (32 kDa protein) (Putative helicase) (3.6.4.-) (58 kDa protein) (Membrane-binding protein) (NTP-binding protein) (NTB) (Viral genome-linked protein) (VPg) (Picornain 3C-like protease) (3C-like protease) (3.4.22.- {ECO:0000269|PubMed:16789216}) (24 kDa protein) (RNA-directed RNA polymerase {ECO:0000255|PROSITE-ProRule:PRU00539}) (2.7.7.48 {ECO:0000255|PROSITE-ProRule:PRU00539, ECO:0000269|PubMed:1431806}) (87 kDa protein) Query ID: lcl|Query_801107 Length: 325 Sequences producing significant alignments: Scientific Common Max Total Query E Per. Acc. Description Name Name Taxid Score Score cover Value Ident Len Accession RecName: Full=RNA1 polyprotein; AltName: Full=B RNA polyprotei... Cowpea mosai... NA 928299 673 673 100% 0.0 100.00 1866 P03600.1 RecName: Full=RNA1 polyprotein; AltName: Full=Genome polyprote... Red clover m... NA 12262 219 219 94% 7e-63 38.87 1864 P35930.1 RecName: Full=RNA1 polyprotein; AltName: Full=Genome polyprote... Bean-pod mot... NA 31715 169 169 94% 9e-46 28.89 1850 Q9YJU5.1 RecName: Full=RNA1 polyprotein; AltName: Full=Genome polyprote... Cowpea sever... NA 31716 147 147 77% 6e-38 30.83 1858 P36312.1 Alignments: >RecName: Full=RNA1 polyprotein; AltName: Full=B RNA polyprotein; AltName: Full=Bottom component polyprotein; AltName: Full=Genome polyprotein B; AltName: Full=P1; Contains: RecName: Full=Protease cofactor; AltName: Full=32 kDa protein; Contains: RecName: Full=Putative helicase; AltName: Full=58 kDa protein; AltName: Full=Membrane-binding protein; AltName: Full=NTP-binding protein; Short=NTB; Contains: RecName: Full=Viral genome-linked protein; AltName: Full=VPg; Contains: RecName: Full=Picornain 3C-like protease; Short=3C-like protease; AltName: Full=24 kDa protein; Contains: RecName: Full=RNA-directed RNA polymerase; AltName: Full=87 kDa protein [Cowpea mosaic virus strain SB] Sequence ID: P03600.1 Length: 1866 Range 1: 2 to 326 Score:673 bits(1737), Expect:0.0, Method:Compositional matrix adjust., Identities:325/325(100%), Positives:325/325(100%), Gaps:0/325(0%) Query 1 GLPEYEADSEALLSQLTIEFTPGMTVSSLLAQVTTNDFHSAIEFFAAEKAVDIEGVHYNA 60 GLPEYEADSEALLSQLTIEFTPGMTVSSLLAQVTTNDFHSAIEFFAAEKAVDIEGVHYNA Sbjct 2 GLPEYEADSEALLSQLTIEFTPGMTVSSLLAQVTTNDFHSAIEFFAAEKAVDIEGVHYNA 61 Query 61 YMQQIRKNPSLLRISVVAYAFHVSDMVAETMSYDVYEFLYKHYALFISNLVTRTLRFKEL 120 YMQQIRKNPSLLRISVVAYAFHVSDMVAETMSYDVYEFLYKHYALFISNLVTRTLRFKEL Sbjct 62 YMQQIRKNPSLLRISVVAYAFHVSDMVAETMSYDVYEFLYKHYALFISNLVTRTLRFKEL 121 Query 121 LLFCKQQFLEKMQASIVWAPELEQYLQVEGDAVAQGVSQLLYKMVTWVPTFVRGAVDWSV 180 LLFCKQQFLEKMQASIVWAPELEQYLQVEGDAVAQGVSQLLYKMVTWVPTFVRGAVDWSV Sbjct 122 LLFCKQQFLEKMQASIVWAPELEQYLQVEGDAVAQGVSQLLYKMVTWVPTFVRGAVDWSV 181 Query 181 DAILVSFRKHFEKMVQEYVPMAHRVCSWLSQLWDKIVQWISQASETMGWFLDGCRDLMTW 240 DAILVSFRKHFEKMVQEYVPMAHRVCSWLSQLWDKIVQWISQASETMGWFLDGCRDLMTW Sbjct 182 DAILVSFRKHFEKMVQEYVPMAHRVCSWLSQLWDKIVQWISQASETMGWFLDGCRDLMTW 241 Query 241 GIATLATCSALSLVEKLLVAMGFLVEPFGLSGIFLRTGVVAAACYNYGTNSKGFAEMMAL 300 GIATLATCSALSLVEKLLVAMGFLVEPFGLSGIFLRTGVVAAACYNYGTNSKGFAEMMAL Sbjct 242 GIATLATCSALSLVEKLLVAMGFLVEPFGLSGIFLRTGVVAAACYNYGTNSKGFAEMMAL 301 Query 301 LSLAANCVSTVIVGGFFPGEKDNAQ 325 LSLAANCVSTVIVGGFFPGEKDNAQ Sbjct 302 LSLAANCVSTVIVGGFFPGEKDNAQ 326 >RecName: Full=RNA1 polyprotein; AltName: Full=Genome polyprotein B; AltName: Full=P1; Contains: RecName: Full=Protease cofactor; AltName: Full=32 kDa protein; Contains: RecName: Full=Putative helicase; AltName: Full=58 kDa protein; AltName: Full=Membrane-binding protein; AltName: Full=NTP-binding protein; Short=NTB; Contains: RecName: Full=Viral genome-linked protein; AltName: Full=VPg; Contains: RecName: Full=Picornain 3C-like protease; Short=3C-like protease; AltName: Full=24 kDa protein; Contains: RecName: Full=RNA-directed RNA polymerase; AltName: Full=87 kDa protein [Red clover mottle virus] Sequence ID: P35930.1 Length: 1864 Range 1: 4 to 315 Score:219 bits(557), Expect:7e-63, Method:Compositional matrix adjust., Identities:124/319(39%), Positives:190/319(59%), Gaps:18/319(5%) Query 18 IEFTPGMTVSSLLAQVTTNDFHSAIEFFAAEKAVDIEGVHYNAYMQQIRKNPSLLRISVV 77 + F PG+ V+ ++ QV +N F ++ +A E + M +++ + LL +S+V Sbjct 4 LTFEPGLCVAGIIRQVRSNPFMHVVQAYARTTETYREDIEMTKSMLKLKADEPLLVMSIV 63 Query 78 AYAFHVSDMVAETMSYDVYEFLYKHYALFISNLVTR---TLRFKELLLFCKQQFLEKMQA 134 A A MV + + EFLY YA +S +VT + + + L C++ L K++ Sbjct 64 AAAMDFQTMVMAPIEMEASEFLYGFYAERMSYIVTNRGMSELHEYIQLQCQRHLLVKVEI 123 Query 135 SIVWAPELEQYLQVEGDAVAQG-----VSQLLYKMVTWVPTFVRGAVDWSVDAILVSFRK 189 QYL E + AQG V +L+ + TWVP V+G + WSVDA+L SF++ Sbjct 124 D-------GQYLVQEHEYEAQGFNIKRVKELITDVATWVPKKVKGMIGWSVDAVLDSFQE 176 Query 190 HFEKMVQEYVPMAHRVCSWLSQLWDKIVQWISQASETMGWFLDGCRDLMTWGIATLATCS 249 +F K++ E +PMA +VCSW++ +WD+I WI A M FL GC +L+TWG+ATLA C Sbjct 177 YFYKVITERIPMAMKVCSWVATVWDQIKTWIEDAMTAMSSFLQGCNELLTWGLATLAACC 236 Query 250 ALSLVEKLLVAMGFLVEPFGLSGIFLRTGVVAAACYNYGTNSKGFAEMMALLSLAANCVS 309 AL+++E++L+ M FL E ++GIFLRTGVVAAACY++ + +KGF EMM++LS+A V+ Sbjct 237 ALNVLERILIFMEFLDESIDIAGIFLRTGVVAAACYHFSSTAKGFTEMMSVLSVATTAVA 296 Query 310 TVIVGGFFPGEKD---NAQ 325 V+ +F G K NAQ Sbjct 297 AVVCANYFGGSKTKKVNAQ 315 >RecName: Full=RNA1 polyprotein; AltName: Full=Genome polyprotein B; AltName: Full=P1; Contains: RecName: Full=Protease cofactor; AltName: Full=32 kDa protein; Contains: RecName: Full=Putative helicase; AltName: Full=58 kDa protein; AltName: Full=Membrane-binding protein; AltName: Full=NTP-binding protein; Short=NTB; Contains: RecName: Full=Viral genome-linked protein; AltName: Full=VPg; Contains: RecName: Full=Picornain 3C-like protease; Short=3C-like protease; AltName: Full=24 kDa protein; Contains: RecName: Full=RNA-directed RNA polymerase; AltName: Full=87 kDa protein [Bean-pod mottle virus (strain Kentucky G7)] Sequence ID: Q9YJU5.1 Length: 1850 Range 1: 1 to 306 Score:169 bits(429), Expect:9e-46, Method:Compositional matrix adjust., Identities:91/315(29%), Positives:172/315(54%), Gaps:16/315(5%) Query 18 IEFTPGMTVSSLLAQVTTNDFHSAIE-FFAAEKAVDIEGVHYNAYMQQIRKNPSLLRISV 76 ++F PG +S ++ +N+ + ++ +FA D E + A ++Q NP LL +S Sbjct 1 MKFYPGQNISEIVYHFQSNETANRLDAYFACGCEEDTEVL---ARLKQC--NPRLLHLSY 55 Query 77 VAYAFHVSDMVAETMSYDVYEFLYKHYALFISNLVTRTLRFKELLLFCKQQFLEKMQASI 136 A+ + E M YD E ++ ++ F+ ++V+ + + +L + + F Q + Sbjct 56 AAFCLEMGSHSIEEMEYDDGELIFSYFQNFLLSIVSNSSKTTKLRAYIRSAFAYHFQHFV 115 Query 137 VWAPELEQYLQ-----VEGDAVAQGVSQLLYKMVTWVPTFVRGAVDWSVDAILVSFRKHF 191 E +QY V+ AQG++ L MV W+PT ++ V++ V +++ SF +HF Sbjct 116 ----EFDQYTNDSLNTVDTSVSAQGIADLALSMVRWIPTQIKKVVNFGVGSVIESFSEHF 171 Query 192 EKMVQEYVPMAHRVCSWLSQLWDKIVQWISQASETMGWFLDGCRDLMTWGIATLATCSAL 251 K++ +Y P+ + SW++ +W + +WI +A++ + WFL GC++L WG+ LA+ AL Sbjct 172 NKLLMQYCPIVFQAFSWVNNIWTMVKEWIEEAAKEISWFLQGCKELHAWGMCILASSCAL 231 Query 252 SLVEKLLVAMGFLVEPFGLSGIFLRTGVVAAACYNYGTNS-KGFAEMMALLSLAANCVST 310 LVEK L+++G + E F L G+F+R+ +V A C + T +E++ ++A + ++T Sbjct 232 GLVEKCLISLGMISESFDLVGLFVRSAIVGAFCVSIKTGKFVTNSELITCATIAVSTIAT 291 Query 311 VIVGGFFPGEKDNAQ 325 V+ F P E+ Q Sbjct 292 VMSQAFKPSEEIKGQ 306 >RecName: Full=RNA1 polyprotein; AltName: Full=Genome polyprotein B; AltName: Full=P1; Contains: RecName: Full=Protease cofactor; AltName: Full=32 kDa protein; Contains: RecName: Full=Putative helicase; AltName: Full=58 kDa protein; AltName: Full=Membrane-binding protein; AltName: Full=NTP-binding protein; Short=NTB; Contains: RecName: Full=Viral genome-linked protein; AltName: Full=VPg; Contains: RecName: Full=Picornain 3C-like protease; Short=3C-like protease; AltName: Full=24 kDa protein; Contains: RecName: Full=RNA-directed RNA polymerase; AltName: Full=87 kDa protein [Cowpea severe mosaic virus (strain DG)] Sequence ID: P36312.1 Length: 1858 Range 1: 46 to 298 Score:147 bits(370), Expect:6e-38, Method:Compositional matrix adjust., Identities:78/253(31%), Positives:136/253(53%), Gaps:2/253(0%) Query 66 RKNPSLLRISVVAYAFHVSDMVA-ETMSYDVYEFLYKHYALFISNLVTRTLRFKELLLFC 124 +++ LL +S A F S+ V E + D E +++ + + ++ + L + Sbjct 46 QRHRRLLTLSYGAMCFQFSNSVGDEGIEVDDDELMFEIFDALLRTKISNSKGMTHLYSWM 105 Query 125 KQQFLEKMQASIVWAPELEQYLQVEGDAVAQGVSQLLYKMVTWVPTFVRGAVDWSVDAIL 184 + +L + + L+ + AQG+SQ + + W+P+ V+ ++ + I+ Sbjct 106 RGVYLSTFKVEVQCDDYNSNLLEKDLAGEAQGLSQFVSGLADWIPSRVKTLAGYAAEGII 165 Query 185 VSFRKHFEKMVQEYVPMAHRVCSWLSQLWDKIVQWISQASETMGWFLDGCRDLMTWGIAT 244 +F+KHF+K++ EY PMA CSW++ +W I +W+ A + M W + GC +L++WG+ Sbjct 166 EAFKKHFDKLLVEYCPMAVAACSWITTVWTTIKEWVQSAMDAMSWIMAGCTELISWGMCV 225 Query 245 LATCSALSLVEKLLVAMGFLVEPFGLSGIFLRTGVVAAACYN-YGTNSKGFAEMMALLSL 303 +A ALSL+EK LVAMG + F L+GIF+R+ VV A C S+ AE++ L+SL Sbjct 226 IAGSCALSLLEKALVAMGLISSSFDLAGIFVRSAVVGAFCLTVVNKRSRNCAELLQLVSL 285 Query 304 AANCVSTVIVGGF 316 A VS+ F Sbjct 286 AVGAVSSATSSCF 298