FastQCFastQC Report
Вт 27 дек 2016
SRR4240379.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4240379.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7400155
Sequences flagged as poor quality0
Sequence length36
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA255460.34520898548746615Illumina Single End Adapter 1 (100% over 33bp)
GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGAT221730.29962885912524806Illumina Single End Adapter 1 (100% over 33bp)
GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTAGAT178660.24142737550767518Illumina Single End Adapter 1 (96% over 32bp)
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN80270.10847070095153413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTCG125050.028.9108169
AGAGCTC125950.028.7052148
CGGAAGA133550.028.3771824
TCGGAAG137250.028.273943
GAAGAGC130150.028.0295016
GGAAGAG135400.027.8141675
CGTATGC123750.027.5011114
AGCTCGT127400.027.50081310
GCTCGTA126900.027.4659611
TGCCGTC120950.027.36367618
AAGAGCT133350.027.347847
GCCGTCT121350.027.29530119
GATCGGA142950.027.147911
ATGCCGT122500.026.9501817
TCGTATG129400.026.8371313
TATGCCG125750.026.81311216
CTCGTAT130300.026.7692812
GTATGCC126450.026.76913815
ATCGGAA144550.026.753132
CCGTCTT124700.026.5944120
Produced by FastQC (version 0.11.2)