TBLASTN 2.8.1+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: X5.fasta
           1,868 sequences; 23,962,143 total letters



Query= KPYK_ENCCU Q8SQP0 Pyruvate kinase (PK) (2.7.1.40)

Length=428
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

  scaffold-361                                                        269     4e-80
  scaffold-115                                                        268     1e-79


> scaffold-361
Length=455836

 Score = 269 bits (688),  Expect = 4e-80, Method: Compositional matrix adjust.
 Identities = 162/437 (37%), Positives = 244/437 (56%), Gaps = 45/437 (10%)
 Frame = +3

Query  12      IGPRTSSREKIKELIDAGMSIARLNFSHGSREAHLEVIRNIRDSRS-GAGRHVSIALDTR  70
               +GP+T+S + + +L  AGM+I R+NFSHGS E H  VI+N RDS    +GR V+IALDT+
Sbjct  218580  VGPKTNSVDMMCKLRQAGMNIVRMNFSHGSYEYHGSVIKNARDSFQ*MSGRPVAIALDTK  218759

Query  71      GPEVRLRTPEMK---DIKVEGGEVLRFSLLSSEKD------IWIPGVDL-KSLGVDNRVF  120
               GPE+R  T  +K   ++ +  G  +  +   +  +      I+I    L  S  V   +F
Sbjct  218760  GPEIR--TGRIKGD*ELSIPHGHEMVLTTDPARANDGDMDCIYIDYEKLPHSTDVGKYIF  218933

Query  121     IDDGAIELRVVNVEEDGFECEVLNSGMIKSNKSMNFPGTDIGDRALGDEDKNDIAFGLEN  180
               +DDG +  +VV ++         NS  I +NK +N P T +   AL + DK D+ FG+EN
Sbjct  218934  VDDGQLRFQVVGIQGKDVRV*AQNSWKISNNKGVNLPMTTVELPALSERDKRDLQFGIEN  219113

Query  181     GIDMVFASFVSCRADVEEIRRLV-------GSKVPVVSKIESCLGMRNLKEIALCSDGVM  233
                +DM+FASF+  + D+ EIR+++       G  + +++KIES  G+ N  EI   +DG+M
Sbjct  219114  DVDMIFASFIRKKEDILEIRQILDNFMNGRGKDIKIIAKIESHEGLDNFDEILQVTDGIM  219293

Query  234     IARGDLGVEIGLENMFSAQKRILYEVKREGRPVICATQMMESMTLKNAPNRSEISDVGNA  293
               +ARGDLG+EI  E +F AQK ++ +    G+P+ICATQM+E+MT    P R+E+SDV NA
Sbjct  219294  VARGDLGIEIAAEKVFIAQKMMIAKCNAVGKPIICATQMLETMTYNPRPTRAEVSDVANA  219473

Query  294     VLDGCDCVMLSAESAVGMFPVETVKFMRSICADAERY-----------DMESRKGAGACG  342
               VLDG DCVMLS E+A G +P+ETV  M  +C +AE             D+  +       
Sbjct  219474  VLDGADCVMLSGETAKGQYPLETVDMMNRLCKEAESVLFYPQLFNELRDVTPKPTTTTEA  219653

Query  343     VSSYV---------DGVVICSGTESQIEKIYLSKPETPIIVISESLWILRRFSIYRGIIP  393
               ++S             + + + T      I   +P  P+I ++ +  + R+  +YRG  P
Sbjct  219654  IASSAVNAAEE*GAAAICVLTTTGESARLISKYRPHIPVITVTRNAKVARQCHLYRGCYP  219833

Query  394     -VYGK----GSEDAEAT  405
                VY K    GSE A A+
Sbjct  219834  FVYEKARKQGSESAPAS  219884


> scaffold-115
Length=160031

 Score = 268 bits (684),  Expect = 1e-79, Method: Compositional matrix adjust.
 Identities = 162/437 (37%), Positives = 247/437 (57%), Gaps = 45/437 (10%)
 Frame = -1

Query  12     IGPRTSSREKIKELIDAGMSIARLNFSHGSREAHLEVIRNIRDSRS-GAGRHVSIALDTR  70
              +GP+T+S + + +L  AGM+I R+NFSHGS E H  VI+N RDS    +GR V+IALDT+
Sbjct  32951  VGPKTNSVDMMCKLRQAGMNIVRMNFSHGSYEYHGSVIKNARDSFQ*MSGRPVAIALDTK  32772

Query  71     GPEVRLRTPEMK---DIKVEGGEVLRFSL---LSSEKDIWIPGVDLKSL----GVDNRVF  120
              GPE+R  T  +K   ++ +  G  +  +     +++ DI    +D + L     V   +F
Sbjct  32771  GPEIR--TGRIKGD*ELSIPHGHEMVLTTDPARANDGDIDCIYIDYEKLPHSTDVGKYIF  32598

Query  121    IDDGAIELRVVNVEEDGFECEVLNSGMIKSNKSMNFPGTDIGDRALGDEDKNDIAFGLEN  180
              +DDG +  +VV+++         NS  I +NK +N P T +   AL + DK D+ FG+EN
Sbjct  32597  VDDGQLRFQVVSIQGREVRV*AQNSWKISNNKGVNLPMTTVELPALSERDKRDLQFGIEN  32418

Query  181    GIDMVFASFVSCRADVEEIRRLV-------GSKVPVVSKIESCLGMRNLKEIALCSDGVM  233
               +DM+FASF+  + D+ EIR+++       G  + +++KIES  G+ N  EI   +DG+M
Sbjct  32417  DVDMIFASFIRKKEDILEIRQILDTFMNGRGKDIKIIAKIESHEGLDNFDEILQVTDGIM  32238

Query  234    IARGDLGVEIGLENMFSAQKRILYEVKREGRPVICATQMMESMTLKNAPNRSEISDVGNA  293
              +ARGDLG+EI  E +F AQK ++ +    G+P+ICATQM+E+MT    P R+E+SDV NA
Sbjct  32237  VARGDLGIEIAAEKVFIAQKMMIAKCNAVGKPIICATQMLETMTYNPRPTRAEVSDVANA  32058

Query  294    VLDGCDCVMLSAESAVGMFPVETVKFMRSICADAERY-----------DMESRKGAGACG  342
              VLDG DCVMLS E+A G +P+ETV  M  +C +AE             D+  +  +    
Sbjct  32057  VLDGADCVMLSGETAKGQYPLETVDMMNRLCKEAESVLFYPQLFNELRDVTPKPTSTTEA  31878

Query  343    VSSYV---------DGVVICSGTESQIEKIYLSKPETPIIVISESLWILRRFSIYRGIIP  393
              ++S             + + + T      I   +P  P+I ++ +  + R   +YRG  P
Sbjct  31877  IASSAVNAAEE*GAAAICVLTTTGESARLISKYRPHIPVITVTRNAKVAR*CHLYRGCYP  31698

Query  394    -VYGK----GSEDAEAT  405
               VY K    GSE A A+
Sbjct  31697  FVYEKARKQGSESAPAS  31647



Lambda      K        H        a         alpha
   0.319    0.136    0.388    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 2550179151


  Database: X5.fasta
    Posted date:  Dec 25, 2021  2:56 PM
  Number of letters in database: 23,962,143
  Number of sequences in database:  1,868



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40