TBLASTN 2.8.1+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: X5.fasta 1,868 sequences; 23,962,143 total letters Query= SYS_ENCCU Q8SS48 Serine--tRNA ligase (6.1.1.11) (Seryl-tRNA synthetase) (SerRS) (Seryl-tRNA(Ser/Sec) synthetase) Length=429 Score E Sequences producing significant alignments: (Bits) Value scaffold-693 350 3e-108 scaffold-170 349 1e-107 > scaffold-693 Length=1268102 Score = 350 bits (899), Expect = 3e-108, Method: Compositional matrix adjust. Identities = 201/436 (46%), Positives = 275/436 (63%), Gaps = 21/436 (5%) Frame = +1 Query 1 MIDINLIRDPK--TREKVVESEKKRFRDGLAVGKAYELDRKRIEMNFRLDQINTRINQLN 58 M+DIN R K +K+ ES+++R V + LD + +++ F LD+ N +N+L Sbjct 687862 MLDINEFRVEKGGNPDKIRESQRRRNA*VELVDQVIALD*EWVKLKFVLDEKNKDLNKL* 688041 Query 59 REIKSGYRQGKNKEDGDLSEKVAEIKGLSDEAKGLRDDVKAVEDELNKVMKGIGNIISPS 118 +EI G R + DL + E++ D+ K + D+ E E + + IGN++ S Sbjct 688042 KEI--GMRMKAKESAEDLL*QKKEMEKARDQQKAIVDEK---ERERDTSLLLIGNLVHDS 688206 Query 119 VVVSNDEKDNPIVRSYRSSRNMQKNPRPFCVLMKDFTHSVAG------AKVMGHRGYYLS 172 V VS DE +N +V+ + + +K R + S+AG +++ GHRGYYL Sbjct 688207 VPVSADENNNAVVKVHPEGKTPEK--RENILAHDQVLESLAGYDSERGSRIAGHRGYYLM 688380 Query 173 GKMARLAQALTRYAIDFLENKGYTYIQTPVMLRRDVMRKTSQLSDFDDQLYKVE---DDL 229 L AL +Y + FL N+G+ I TP + +DVM KT+QL++FD+ LYKV D+ Sbjct 688381 NDGVDLNLALIQYGLQFLRNRGFNKISTPFFMNKDVMSKTAQLNEFDETLYKVSGDTDEK 688560 Query 230 YLIATSEQSLAALYMDERM-VPQE-VPKKFCGQSLCFRKEAGAHGKDNAGLFRVHQFEKI 287 YLIATSEQ ++A +M+E P E +P K+ G S CFRKEAGAHGKDN GLFRVHQFEKI Sbjct 688561 YLIATSEQPISAFHMNECFDKPAE*LPIKYAGYSTCFRKEAGAHGKDNWGLFRVHQFEKI 688740 Query 288 EQFVICGPEESQKYHEEMIKACEEFYQSLDISYNVVGIVSGELNDAAAIKYDLEAYFPSA 347 EQFV+ P +S + EEMI EEF +SL + Y VV IVSG LN+AAA KYDLEA+FP Sbjct 688741 EQFVLTEPGKSWEMLEEMIGTSEEFMKSLGLQYRVVSIVSGALNNAAAKKYDLEAWFPYQ 688920 Query 348 EKYRELVSCSNCTDYQSRELEIRYGVVKENNR-KIYVHLLNGTMCAVQRALCCIVENYQT 406 YRELVSCSNCTD+QSR +EIR G K N++ K YVH LN T+CA +R +CC++ENYQT Sbjct 688921 GVYRELVSCSNCTDFQSRRMEIRCGAKK*NSQEKKYVHCLNSTLCATERTMCCLLENYQT 689100 Query 407 GDGIAIPDVLQGYFGG 422 DG+ +P+VL+ Y GG Sbjct 689101 EDGVVVPEVLRPYMGG 689148 > scaffold-170 Length=449377 Score = 349 bits (895), Expect = 1e-107, Method: Compositional matrix adjust. Identities = 200/436 (46%), Positives = 277/436 (64%), Gaps = 21/436 (5%) Frame = +1 Query 1 MIDINLIRDPK--TREKVVESEKKRFRDGLAVGKAYELDRKRIEMNFRLDQINTRINQLN 58 M+DIN R K +K+ ES+++R + V + LD++ +++ F LD+ N +N+L Sbjct 113467 MLDINEFRVDKGGNPDKIRESQRRRNANVELVDQVIALDQEWVKLKFILDEKNKDLNKL* 113646 Query 59 REIKSGYRQGKNKEDGDLSEKVAEIKGLSDEAKGLRDDVKAVEDELNKVMKGIGNIISPS 118 +EI G R + DL +++ D+ K + D+ E E + + IGN++ S Sbjct 113647 KEI--GMRMKAKESAEDLL**KKDMEKARDQQKAIVDEK---ERERDTSLLLIGNLVHDS 113811 Query 119 VVVSNDEKDNPIVRSYRSSRNMQKNPRPFCVLMKDFTHSVAG------AKVMGHRGYYLS 172 V +S DE +N +V+ + + +K R + S+AG +++ GHRGYYL Sbjct 113812 VPISADENNNAVVKVHPEGKTPEK--RENILAHDQVLESLAGYDSERGSRIAGHRGYYLM 113985 Query 173 GKMARLAQALTRYAIDFLENKGYTYIQTPVMLRRDVMRKTSQLSDFDDQLYKVE---DDL 229 L AL +Y + FL N+G+T I TP + +DVM KT+QL++FD+ LYKV D+ Sbjct 113986 NDGVDLNLALIQYGL*FLRNRGFTKISTPFFMNKDVMSKTAQLNEFDETLYKVSGDTDEK 114165 Query 230 YLIATSEQSLAALYMDERM-VPQE-VPKKFCGQSLCFRKEAGAHGKDNAGLFRVHQFEKI 287 YLIATSEQ ++A +M+E P E +P K+ G S CFRKEAGAHGKDN GLFRVHQFEKI Sbjct 114166 YLIATSEQPISAFHMNECFDKPAE*LPIKYAGYSTCFRKEAGAHGKDNWGLFRVHQFEKI 114345 Query 288 EQFVICGPEESQKYHEEMIKACEEFYQSLDISYNVVGIVSGELNDAAAIKYDLEAYFPSA 347 EQFV+ P +S + EEMI EEF +SL + Y VV IVSG LN+AAA KYDLEA+FP Sbjct 114346 EQFVLTEPGKSWEMLEEMIGTSEEFMKSLGL*YRVVSIVSGALNNAAAKKYDLEAWFPYQ 114525 Query 348 EKYRELVSCSNCTDYQSRELEIRYGVVKENNR-KIYVHLLNGTMCAVQRALCCIVENYQT 406 YRELVSCSNCTD+QSR +EIR G K N++ K YVH LN T+CA +R +CC++ENYQT Sbjct 114526 GVYRELVSCSNCTDFQSRRMEIRCGAKK*NSQEKKYVHCLNSTLCATERTMCCLLENYQT 114705 Query 407 GDGIAIPDVLQGYFGG 422 DG+ +P+VL+ Y GG Sbjct 114706 EDGVVVPEVLRPYMGG 114753 Lambda K H a alpha 0.318 0.136 0.389 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 2557977864 Database: X5.fasta Posted date: Dec 25, 2021 2:56 PM Number of letters in database: 23,962,143 Number of sequences in database: 1,868 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 13 Window for multiple hits: 40